• Microbiology and Biotechnology Letters
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• v.17 no.3
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• pp.224-230
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• 1989

For the production of catechol from benzene, bacteria capable of assimilating benzene as a sole carbon and energy source were isolated from soils. Among them, newly isolated strain, KY-114 hay-ing the best ability of producing catechol from benzene was selected and a mutant Pseudomonas sp. HW-103 was developed from Pseudomonas sp. KY-114 by using mutagenesis induced by N-methyl - N'- nitro - N -nitrobo guanidine. The catechol reduction from benzone by Pseudomonas sp. HW-103 was investigated under various conditions. The highest catechol concentration (0.61 g/$\ell$) was obtained in the growth medium (pH 6.5) containing 1% sodium citrate, 0.75% (NH$_4$)$_2$SO$_4$, 0.15% benzene and other minerals at 3$0^{\circ}C$ after incubating of 15hrs. In the catechol production through the reaction with resting rolls, 2.5 g/1 of catechol was produced from 4 g/$\ell$ of benzene after incubation of 10 hrs under the optium conditions, which correponds to 45% of theoretical catechol yield.

• Journal of Power System Engineering
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• v.9 no.4
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• pp.96-101
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• 2005

Magnesium alloys have been widely used for many structural components of automobiles and aircraft because of high specific strength and good cast ability in spite of hexagonal closed-packed crystal structure of pure magnesium. In this study, uniaxial tension tests at high temperature and creep tests are done in order to investigate the characteristics of high temperature and mechanisms for creep deformation of AZ31 Mg alloy. Yield stress and ultimate tensile stress decreased with increasing temperature, but elongation increased from results of uniaxial tension test at high temperature. The apparent activation energy Qc, the applied stress exponent n and rupture life have been determined during creep of AZ31 Mg alloy over the temperature range of 473K to 573K and stress range of 23.42 MPa to 93.59 MPa, respectively, in order to investigate the creep behavior. Constant load creep tests were carried out in the equipment including automatic temperature controller, whose data are sent to computer. At around the temperature of $473K{\sim}493K$ and under the stress level of $62.43{\sim}93.59%MPa$, and again at around the temperature of $553K{\sim}573K$ and under the stress level of $23.42{\sim}39.00MPa$, the creep behavior obeyed a simple power-law relating steady state creep rate to applied stress and the activation energy for the creep deformation was nearly equal, respectively, and a little low to that of the self diffusion of Mg alloy including aluminum. Also rupture surfaces at high temperature have had bigger dimples than those at lower temperature by SEM.

• Proceedings of the KWS Conference
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• 2009.11a
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• pp.103-103
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• 2009

As the size of containership increased over 14,000TEU, thick steel plate with high strength has been used. The plate thickness increased over 70mm and yield strength of the steel plate was around $47kg_f/mm^2$. Many researchers reported that the thick welded plate has low crack arrest toughness. They noticed the crack arrest ability is dependent on the plate thickness. In other words, brittle crack propagates straightly along the welded line and make abrupt fracture in the thick plate which causes low $K_{ca}$. In this study, the other factors, especially welding heat input, to cause low crack arrest toughness was investigated for thick steel plate welds. EH grade steel plates were used in this study and 50 to 80 thick plates were tested to confirm thickness sensitivity. Electro gas welding (EGW) and flux cored arc welding (FCAW) were adopted to prepare the welded joints. Temperature gradient ESSO test was performed to measure $K_{ca}$ values with the variation of welding variables. As a result of this study, regardless of plate thickness, welding heat input to cause welding residual stress around crack path is a key factor to control the brittle crack propagation in welded joints.

• Water Engineering Research
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• v.3 no.1
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• pp.57-62
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• 2002

This research is intended to integrate long-term operation rules and real time operation policy for conservation & flood control in a reservoir. The familiar Yield model has been modified and used to provide long-term rule curves. The model employs linear programming technique under given physical conditions, i.e., total capacity, dead storage, spillways, outlet capacity and their respective elevations to find required and desired minimum storage fur different demands. To investigate the system behavior resulting from the above-mentioned operating policy, i.e., the rule curves, the simulation model was used. Results of the simulation model show that the results of the optimization model are indeed valid. After confirmation of the above mentioned rule curves by the simulation models, gate operation procedure was merged with the long term operation rules to determine the optimum reservoir operating policy. In the gate operation procedure, operating policy in downstream flood plain, i.e., determination of damaging and non-damaging discharges in flood plain, peak floods, which could be routed by reservoir, are determined. Also outflow hydrograph and variations of water surface levels for two known hydrographs are determined. To examine efficiency of the above-mentioned models and their ability in determining the optimum operation policy, Esteghlal reservoir in Iran was analyzed as a case study. A numerical model fur the solution of two-dimensional dam break problems using fractional step method is developed on unstructured grid. The model is based on second-order Weighted Averaged Flux(WAF) scheme with HLLC approximate Riemann solver. To control the nonphysical oscillations associated with second-order accuracy, TVD scheme with SUPERBEE limiter is used. The developed model is verified by comparing the computational solutions with analytic solutions in idealized test cases. Very good agreements have been achieved in the verifications.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.1
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• pp.23-27
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• 1998

Inhibition of nodule formation and nitrogen fixation by soil nitrogen, primarily nitrate, is well known in legume plants. The present study was undertaken to evaluate the effect of ${NO_3}^-$ on the nodulation, nitrogenase activity, and growth of supernodulating soybean mutant and its wild type. A greenhouse study was conducted to compare two of supernodulating mutants, 'SS2-2' and 'nts 382', with the normal nodulating cultivar 'Sinpaldalkong 2' when grown in a 1-l styroform cup filled with sand, and fertilized with five levels of ${NO_3}^-$ (0, 2, 4, 8, and 12 mM). During the growth period, each plant was supplied two or three times a week with 50 mL of nutrient solution. Supernodulating soybean mutants, SS2-2 and nts 382, showed more nodules and nodule mass, and greater $C_2\;H_2$ activity than the wild type, Sinpaldalkong 2, regardless of the level of exogeneous nitrogen supply. On the other hand, total dry weight of SS2-2 mutant, which was smaller than Sinpaldalkong 2, did not respond to the various ${NO_3}^-$-N levels. This suggested that supernodulating SS2-2 mutant could maintain fairly high total dry weight at the low ${NO_3}^-$-N level, even in the absence of exogeneous ${NO_3}^-$-N in the nutrient solution. From the reduced top growth and high nitrogen fixing ability of supernodulating mutants, it was surmised that supernodulating mutant could potentially protect agricultural environments from pollution through the reduction in nitrogen fertilization as well as maintain fairly high yield with increasing planting density.

• Microbiology and Biotechnology Letters
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• v.20 no.4
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• pp.477-482
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• 1992

A bacterial strain Acinetobacter calcoaceticus was examined for its ability to degrade fats, oils and hydrocarbons, and tested for the possibility of application in wastewater treatment. All fats and oils tested were degraded by the strain. About 60% of hexadecane, 26% of fish oiL and 40-54% of vegetable oils were consumed respectively in shaking-flask culture. Saturated fatty acid compositions were about 55% in fish oil and 6-12% in vegetable oils. Increases in cell mass were accompanied with decreases in the concentrations of carbon sources. When jar fermentor in place of shaking-flask was used as a culturing vessel. above 80% of all carbon sources was consumed and yield of cell mass was improved to nearly 1.00. Synthetic wastewaters containing 3% of fat, oil, or hydrocarbon as a sale ca,bon source were treated sequentially with A. calcoaceticus first and then exposed to activated sludge. The concentrations of carbon sources were decreased below 0.06% through the process, and the concentrations of suspended solids were lower than 53 mglml. The data imply the potential use of A. calcoaceticus in wastewater treatment.

• Korean Journal of Food Science and Technology
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• v.27 no.6
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• pp.839-844
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• 1995

Appropriate types and concentrations of starch-based fat replacers which are low-pH stable, shear resistant and heat stable were selected to develop reduced-fat mayonnaise without affecting taste, texture and emulsion stability. Four kinds of commercial fat replacers [Neutral DR (DR), Stellar 100X (SX), Sta Slim 150 (SS) and N-Lite L (NL)] were thermally activated after appropriately hydrated and applied to mayonnaise formulation. For each fat replacer, eight recipes having various ratios of soybean oil/fat replacer(95/5 to 60/40) were formulated and compared with standard formulation(100% soybean oil) using rheological and sensory evaluations. All tested starch pastes showed pseudoplastic behavior, time-dependent thixotropic gel characteristics. The increasing order of pseudoplasticity and yield stress was RM, NL, SS and ST. The hardness and adhesiveness of each fat replacer-added mayonnaise was increased in the same order as above. Formulated mayonnaise which contained higher ratio of fat replacer showed lower values in hardness and adhesiveness. ST at up to 25% oil replacement exhibited the highest oil replacing ability among four replacers. ST-added mayonnaise also exhibited the highest sensory score and emulsion stability among all products including standard formula. The high sensory score in spreadability of RM-added mayonnaise well reflected the consistent index of RM paste. Use of fat replacers reduced oily odor and greasy aftertaste of mayonnaise.

• Korean Journal of Food Science and Technology
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• v.32 no.3
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• pp.736-741
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• 2000

The abilities of petroleum ether-extracts prepared from 75 plants to inhibit tyrosinase activity were evaluated in reverse micelles composed of isooctane/AOT(100 mM)/phosphate buffer(20 mM, pH 8.0) containing tyrosinase(105.3 units/mL) and 3,4-dihydroxyphenylalanine(0.18 mM). Compared with control which has no plant extracts, garlic could completely inhibit in vitro melanogenesis by tyrosinase, and Chinese quince, sweet potato, onion, radish bud and apple did more than 60%. Lipophilic extracts of medicinal plants and herbs such as rosemary, coriander, cinnamomi ramulus, crataegii fructus, ramulus biotae folium, mume fructus, menthae herba, eucommiae cortex and clove also inhibited tyrosinase activity more than 60%. When the extraction yield of lipophilic materials was considered together with their inhibition effect on tyrosinase, it was possible to select plants of which tyrosinase inhibitors could be produced in high quantity from unit weight. Using reverse micelles, the analysis of the capacity of lipophilic materials to inhibit tyrosinase activity which was difficult up to present could be possible.

• Journal of the Korean Chemical Society
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• v.36 no.1
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• pp.81-86
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• 1992

The electrophilic and nucleophilic reactions of M(S-S,ph)(N-N,H) (M = Ni(II), Pd(II), Pt(II); (S-S,ph) = 1,2-diphenylethylenedithiolate; (N-N,H)=1,10-phenanthroline) complexes have been investigated. Reaction with norbornadiene depended upon the back donating ability of the central metal ion and produced 2,5-dithia-3,4-diphenyl-tricyclo[4,4,1,0]-undeca-3,8-diene. In the reaction with methyl iodide, the effect of cleavage of (N-N,H) ligand affected the yield of methylated $M(S-S,ph)_2$ product. The structure of the thermolysis product, ${\alpha},{\alpha}{\prime}$-bismethylthiostibene $(CH_3S-SCH_3,ph)$ of methylated complexes indicates that the main product of the nucleophilic reaction is $M(CH_3S-SCH_3,ph)(S-S,ph)$. We have synthesized a new mixed-ligand complex M(S-S,CN)(N-N,H)((S-S,CN) = 1,2-dicyanoethylenedithiolate) through the nucleophilic reaction of ligand.

• Food Science of Animal Resources
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• v.35 no.5
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• pp.585-596
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• 2015

Chicken breast dipped with citric acid (CA) was treated by sous vide processing and stored in a refrigerated state for 0, 3, 6, 9, and 14 d. A non-dipped control group (CON) and three groups dipped in different concentrations of citric acid concentration were analyzed (0.5%, 0.5CIT; 2.0%, 2CIT and 5.0%, 5CIT; w/v). Cooking yield and moisture content increased due to the citric acid. While the redness of the juice and meat in all groups showed significant increase during storage, the redness of the citric acid groups was reduced compared to the control group (p<0.05). The percentage of myoglobin denaturation (PMD) of the CA groups was also increased according to the level of CA during storage. Total aerobic counts, Enterobacteriaceae counts, volatile basic nitrogen and thiobarbituric acid reactive substances (TBARS) were generally lower in the citric acid-treated samples than in untreated ones, indicating extended shelf life of the cooked chicken breast dipped in citric acid solution. The shear force of the 2CIT and 5CIT groups was significantly lower (p<0.05). The findings indicated positive effects in the physicochemical properties and storage ability of sous vide chicken breast at 2% and 5% citric acid concentrations.