• 한국소음진동공학회논문집
• /
• 제22권1호
• /
• pp.81-87
• /
• 2012

This paper establishes a modeling and simulation procedure for structural response and reliability of a cylindrical array sensor on submarines under the shock generated by underwater explosion. The structural reliability of SONAR is important because the submarine could get out of combat ability by the structural damage of the SONAR upon explosion. A cylindrical array sensor was first modeled using the finite element method. Modal analysis was then performed for the check of the reliability of the modeling. The shock resistance simulations were performed for the responses to the structural shock waves and for the responses to the directly applied underwater shock waves, according to BV-043 and MIL-STD-901D, respectively. The stresses of the structure were evaluated with von-Mises scheme. Vulnerable regions were exposed through mapping the maximum stress to the structural model. Maximum stress of the SONAR was compared with the yield stress of the material to examine the structural reliability.

• 미생물과산업
• /
• 제19권2호
• /
• pp.34-41
• /
• 1993

Industrial strain improvement is concerned with developing or modifying microorganisms used in production of commercially important fermentation products. The aim is to reduce the production cost by improving productivity of a strain and manipulating specific characteristics such as the ability to utilize cheaper raw materials or resist bacteriophages. The traditional empirical approach to strain improvement is mutation combined with selection and breeding techniques. It is still used by us to improve the productivity of organisms in amino acids, organic acids and enzymes production. The breeding of high L-lysine-producing strain Au112 is one of the outstanding examples of this approach. It is a homoserine auxotroph with AEC, TA double metabolic analogue resistant markers. The yield reaches 100 g/l. Besides, the citric acid-producing organism Aspergillus niger, Co827, its productivity reaches the advanced level in the world, is also the result of a series mutations especially with $^60Co{\gamma}$-radiation. The thermostable .alpha.-amylase producing strain A 4041 is the third example. By combining physical and chemical mutations, the strain A 4041 becomes an asporogenous, catabolite derepressed mutant with rifamycin resistant and methionine, arginine auxotroph markers. The .alpha.-amylase activity reaches 200 units/ml. The fourth successful example of mutation in strain improvement is the glucoamylase-producing strain Aspergillus niger SP56, its enzyme activity is 20,000 units/ml, 4 times of that of the parental strain UV-11. Recently, recombinant DNA approach provides a worthwhile alternative strategy to industrial strain improvement. This technique had been used by us to increase the thermostable .alpha.-amylase production and on some genetic researches.

• 생약학회지
• /
• 제27권4호
• /
• pp.289-294
• /
• 1996

In previous paper, we described the induced callus of Corydalis remota contains a significant amount of alkaloids. This study describes an optimal condition to maximize alkaloid production. The suspension cultures maintained alkaloid production ability after fifth subculture and a small amount of alkaloid seemed to be released out of cells. The yields of alkaloid by cultured cells was varied depending on the concentrations of NAA, carbon sources and phosphate ion and depending on the vitamin combinations and concentrations. Biosynthetic precursor and an elicitor treatment also affected the total alkaloid yield of the cultures. The optimal conditions for alkaloid production were as follows: 1) MS basal salt containing 30 g/l of glucose, 1.0 mg/l of NAA, and vitamins of LS medium should be used. 2) The culture should be treated with tyrosine 20 mg/l, and yeast extract 1.5 ml/l after the culture reached a stationary phase of growth. Five alkaloids were isolated from the cultures and they were characterized. The spectral data unambiguously revealed that the isolated compounds were dihydrosanguinarine, protopine. tetrahydropalmatine, allocyptopine and ambinine, respectively.

• 전기학회논문지P
• /
• 제64권3호
• /
• pp.153-158
• /
• 2015

Lights from cities have been a grateful outcome of industrialization and a barometer of how developed the city is. However, indiscriminate exposure to artificial lights caused displeasure to people, ecological crisis to the nature, and overuse of energy. for example, excessive light is cause serious diseases such as sleep disorders, cancer in human, declining populations caused by reducing the ability to reproduce for animals and Plant yield is reduced. Therefore, each local governments have came up with ordinances in accordance with the law "Light Pollution Prevention Act". Accordingly, we've analyzed current Chungcheongbuk-do's current states investigation and reduction method proposed for the light pollution act.

• Journal of Microbiology and Biotechnology
• /
• 제19권5호
• /
• pp.474-481
• /
• 2009

A novel microorganism, designated as LG12, was isolated from soil based on its ability to use acrylic acid as the sole carbon source. An electron microscopic analysis of its morphological characteristics and phylogenetic classification by 16S rRNA homology showed that the LG12 strain belongs to Rhodococcus erythropolis. R. erythropolis LG12 was able to metabolize a high concentration of acrylic acid (up to 40 g/l). In addition, R. erythropolis LG12 exhibited the highest acrylic acid-degrading activity among the tested microorganisms, including R. rhodochrous, R. equi, R. rubber, Candida rugosa, and Bacillus cereus. The effect of the culture conditions of R. erythropo/is LG12 on the production of 3-hydroxypropionic acid (3HP) from acrylic acid was also examined. To enhance the production of 3HP, acrylic acid-assimilating activity was induced by adding 1 mM acrylic acid to the culture medium when the cell density reached an $OD_{600}$ of 5. Further cultivation of R. erythropo/is LG 12 with 40 g/l of acrylic acid resulted in the production of 17.5 g/l of 3HP with a molar conversion yield of 44% and productivity of 0.22 g/l/h at $30^{\circ}C$ after 72 h.

• 한국초지조사료학회지
• /
• 제15권4호
• /
• pp.265-273
• /
• 1995

Seeds of chicory were collected and selected with good germination, emergence and productivity. To develop chicory as a new forage crop seeds were primed with polyethylene glycol (PEG 6000) and coated with some chemicals, and tested in laboratory and field. The results obtained are summarized as follows: 1. Among the seed lines collected, PUlgAH, PU21TH, PU37CH and PU30TK were germinated more quickly and took less time for germination than the others. 2. PUI8AH was the best in emergence and growth although PU21TH showed superior germinative ability among the lines collected. 3. Chicory seeds primed with 20~25% PEG solution for three days at $10^{\circ}C$, or the seeds treated with 25% PEG solution at $15^{\circ}C$ regardless the treatment period showed good germination performances. 4. Osmotic priming with PEG accelerated the germination of chicory, but not in the field. 5. Coated seeds geminated more slowly in Petridishes but showed superior emergence and growth to those of primed or untreated seeds. Treatment 3 showed the best performances in pot experiment. 6. Coated seeds showed remarkable increase in establishment and dry matter yield especially in sod sowing compared to the other sowing method. 7. Most nutrient contents except iron and manganese of chicory were much higher than those of mixed pasture .

• 한국대기환경학회지
• /
• 제19권5호
• /
• pp.583-594
• /
• 2003

Ozone is an important atmospheric pollutant that is occurred in tropospheric chemical process and it also affects the human health and plants. For a correct application of abatement strategies for ozone, it is necessary to understand the factors that control atmospheric ozone removal by dry deposition processes. The present study investigates the numerical simulation of the dry deposition velocity (V$^{d}$ ) obtained from PNU/DEM (Pusan National University Deposition Model). PNU/DEM includes seasonal categories, meteorological factors, surface properties and land-use types and proposes for an accurate numerical computation. And, this study examines the ability of the PNU/DEM to compute V$_{d}$ of ozone over water surfaces and evaluates PNU/DEM by comparing its estimated V$_{d}$ to past observed V$_{d}$ over water. The parametrization was found to yield V$_{d}$ values generally in good agreement with the observations for the deciduous forest and the coniferous forest. Ozone is removed slowly at wet surface or water due to its low water solubility. Therefore V$_{d}$ values over water were lower than Vd values over the other surfaces. Comparison of PNU/DEM simulated V d values to observations of ozone V$_{d}$ that have been reported in the literature implies that PNU/DEM produces realistic results.

• Earthquakes and Structures
• /
• 제15권5호
• /
• pp.487-498
• /
• 2018

Buckling-restrained braces are passive control devices with high level of energy dissipation ability. However, they suffer from low post-yield stiffness which makes them vulnerable to severe ground motions, especially near-field earthquakes. Among the several methods proposed to improve resistance of BRB frames, mega-brace configuration can be a solution to increase frame lateral strength and stiffness and improve distribution of forces to prevent large displacement in braces. Due to the limited number of research regarding the performance of such systems, the current paper aims to assess seismic performance of BRB frames with mega-bracing arrangement under near-field earthquakes via a detailed probabilistic framework. For this purpose, a group of multi-story mega-BRB frames were modelled by OpenSEES software platform. In the first part of the paper, simplified procedures including nonlinear pushover and Incremental Dynamic Analysis were conducted for performance evaluation. Two groups of near-fault seismic ground motions (Non-pulse and Pulse-like records) were considered for analyses to take into account the effects of record-to-record uncertainties, as well as forward directivity on the results. In the second part, seismic reliability analyses are conducted in the context of performance based earthquake engineering. Two widely-known EDP-based and IM-based probabilistic frameworks are employed to estimate collapse potential of the structures. Results show that all the structures can successfully tolerate near-field earthquakes with a high level of confidence level. Therefore, mega-bracing configuration can be an effective alternative to conventional BRB bracing to withstand near-field earthquakes.

• 대한의용생체공학회:의공학회지
• /
• 제30권2호
• /
• pp.122-128
• /
• 2009

Cancer serum biomarkers have advanced our ability to more accurately predict tumor classification, prognostic/metastatic potential, and response potential to novel chemotherapies. Serum amyloid A (SAA) and Vascular endothelial growth factor (VEGF) have potential utility as a serum biomarker for lung cancer. Quantum dots, nanometer-sized crystals, have a high quantum yield, sensitivity, and pronounced photostability. The properties of quantum dots can be efficiently applied to the detection of serum biomarkers in immunoassays as fluorescent probe. We used quantum dots as fluorescent probes in immunoassays and attempted to detect serum amyloid A and vascular endothelial growth factor as serum biomarkers of lung cancer. This fluorescence immunoassay based on the properties of quantum dots is applicable to the detection of serum biomarkers for lung cancer. The fluorescence immunoassay with quantum dots should allow the efficient and specific detection of serum amyloid A (SAA) for the possible diagnosis of lung cancer.

• 한국미생물·생명공학회지
• /
• 제14권5호
• /
• pp.365-369
• /
• 1986

새로운 alcohol발효성 효모 균주 개발을 목적으로 S. diastaticus 와 C. tropicalis간의 세포 융합을 하여 얻은 fusant의 성질에 대해서는 제3보$^{(10)}$에서 발표한 바이다. 본 보에서 는 fusant의 glucoamylase와 pullulanase activity와 glucoamylase의 성질 및 발효력을 조사하였다. glucoamylase activity는 parent보다 fusant가 1.5배 높은 활성을 나타내었고 pullulanase activity 역시 두배의 높은 활성을 나타내었다. glucoamylase의 성질을 조사한 바 온도, pH에서 비슷한 경향을 나타내었으며 발효력을 조사하기 위하여 정치법에서는 기질을 soluble starch로 한 것 보다 liquefied potato starch에서 alcohol 생성력이 2배 증가되었으며 발효력 또한 더 나았다. 15% 액화한 potato starch를 기질로하여 jar-fermenter에서 발효시켰을 때 당화율 94%에서 생성된 alcohol이 7.8% (v/v)이고 소비된 당에 대한 발효율은 78%였다.