• Asian Pacific Journal of Cancer Prevention
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• 제16권10호
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• pp.4347-4351
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• 2015

Background: Previous studies showed that genetic polymorphisms of glutathione S-transferase P1 (GSTP1) were involved in glutathione metabolism and genetic polymorphisms of ribonucleotide reductase (RRM1) were correlated with DNA synthesis. Here we explored the effects of these polymorphisms on the chemosensitivity and clinical outcome in Chinese non-small cell lung cancer (NSCLC) patients treated with gemcitabine-cisplatin regimens. Materials and Methods: DNA sequencing was used to evaluate genetic polymorphisms of GSTP1 Ile105Val and RRM1 C37A-T524C in 47 NSCLC patients treated with gemcitabine-cisplatin regimens. Clinical response was evaluated according to RECIST criteria after 2 cycles of chemotherapy and toxicity was assessed by 1979 WHO criteria (acute and subacute toxicity graduation criteria in chemotherapeutic agents). Results: There was no statistical significance between sensitive and non-sensitive groups regarding the genotype frequency distribution of GSTP1 Ile105Val polymorphism (p>0.05). But for RRM1 C37A-T524C genotype, sensitive group had higher proportion of high effective genotype than non-sensitive group (p=0.009). And according to the joint detection of GSTP1 Ile105Val and RRM1 C37A-T524C polymorphisms, the proportion of type A (A/A + high effective genotype) was significantly higher in sensitive group than in non-sensitive group (p=0.009). Toxicity showed no correlation with the genotypes between two groups (p>0.05). Conclusions: Compared with single detection of genetic polymorphisms of GSTP1 Ile105Val or RRM1 C37A-T524C, joint detection of both may be more helpful for patients with NSCLC to receive gemcitabine-cisplatin regimens as the first-line chemotherapy. Especially, genetic polymorphism of RRM1 is more likely to be used as an important biomarker to predict the response and toxicity of gemcitabine-cisplatin combination chemotherapy in NSCLC.

• 동양고전연구
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• 제48호
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• pp.225-252
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• 2012

본 논문은 주자(朱子)의 중화설(中和說)의 변천과정을 살펴보고 후대의 평가에 대해서 살펴본 것이다. 주자(朱子)는 중화구설(中和舊說)의 단계에서 도남학파(道南學派)로 내려오는 이동(李?)의 학설을 무시하고, 장식(張?)을 만나 호상학파(湖湘學派)의 '선찰식후함양(先察識後涵養)'을 따르게 된다. 그는 이동(李?)으로부터 수양법(修養法)을 배웠지만 장식(張?)과 만나 토론하면서 호상학파(湖湘學派)의 '선찰식후함양(先察識後涵養)'의 방법론(方法論)에 매료되게 된다. 이는 미발(未發)은 없고 모든 것이 발(發)한 이발(已發)의 때만을 인정하는 것이었다. 중화신설(中和新說)의 단계에서는 중화구설(中和舊說) 때의 잘못을 깨닫고 다시 미발(未發)의 때를 중시하게 된다. 심(心) 성(性) 정(情)의 관계도 심통성정(心統性情)이라는 것으로 적립하게 된다. 또한 호굉(胡宏)의 '선찰식후함양(先察識後涵養)'으로부터 벗어나 '경(敬)'의 수양법(修養法)을 내세운다. 이러한 수양(修養) 방법은 호상학파(湖湘學派)의 이발(已發)위주의 수양법(修養法)에서 도남학파(道南學派)의 미발(未發)위주의 수양법(修養法)으로 바뀐 것을 의미한다. 이렇듯 완벽해 보이는 주자(朱子)의 중화설(中和說)은 조선(朝鮮)에 들어와서 16세기 율곡(栗谷)과 우계(牛溪)의 논쟁을 통해, 17세기 우암(尤庵)과 우헌(寓軒)의 논쟁을 통해, 18세기 남당(南塘)과 외암(巍巖)의 호락논쟁(湖洛論爭)을 통해 다시 논의된다. 주자(朱子)의 중화설(中和說)은 미비점을 가지고 있었기 때문에 위와 같은 논쟁을 겪게 된 것이다. 이러한 논쟁은 주자(朱子)의 중화설(中和說)이 미비점을 안고 있기 때문에 벌어진 논쟁이다.

• 동양고전연구
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• 제49호
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• pp.7-38
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• 2012

본 연구는 여헌의 "역학도설"이 사실은 위기지학(爲己之學)의 전통을 계승하는 동시에 그것을 강화하고 발전시키는 저술이라는 점을 논증한 것이다. 그 논증의 방식은 공자로부터 송대로 이어지는 위기지학의 개념화 과정, 그리고 특히 남송 시대의 주희와 그의 문인들에 의하여 시도된 위기지학의 체계화 과정을 논하고, 그것을 바탕으로 여헌의 "역학도설"이 그러한 위기지학 전통의 계승과 발전이라는 맥락에서 이루어진 것임을 밝히는 것이었다. 위기지학은 공자 이래로 유학의 맥락에서 유학적 진실을 지키기 위해서 개발하고 전승하면서 체계화하여 온 실학적 방법론이라는 관점을 채택하였고, 여헌의 "역학도설"은 바로 이러한 실학적 방법론을 발전시켜서 더 실현력이 높은 방법론으로 발전시킨 것이라는 관점을 취한 것이 본 연구이다. 특히 그가 "역학도설"에서 성현의 사업 내지 우주 사업을 강조하는 것이 바로 그렇게 볼 수 있는 점이다. 이러한 논의는 아직까지 위기지학에 관한 연구가 본격화되지 않은 상태에서 이루어진 것이므로, 더 많은 구체적이고 상세한 논의를 필요로 하는 것이다. 그럼에도 불구하고 본 연구를 통해서 제시하고자 했던 여헌의 "역학도설"의 저술적 지향성이나 학문적 내용의 특성에 대해서는 기존의 연구들이 주로 역학이라는 경학적 분야에서 이루어진 성과라는 사실에 초점을 맞추었던 것과 차별화된 관점을 제시하는 데에는 작은 기여를 하였다고 생각된다. 여헌의 "역학도설"은 따라서 역학의 원리와 체제에 근거하여 설계한 위기지학의 교과서로서의 성향과 구도를 두드러지게 갖추고 있는 저술이다. 중국 송대부터 이어지면서 발전해온 위기지학이 여헌의 단계에 이르러서 역학의 방식에 의하여 재구성되었다는 점에 중요한 발전이 있는 것이다. 즉 여헌의 "역학도설"은 경학과 위기지학을 통합하고, 비로소 경학의 지혜를 우주 사업의 역량으로 내면화하고 양성할 수 있는 체계적인 방법을 세웠다는 점에 의의가 있다. 이것은 중국의 송대의 유학자들이 위기지학의 중요성을 절감하면서도 경학의 숲에 매몰되어서 경학과 위기지학의 병행상태에 있었던 것과 다르다. 그리고 조선의 유학자들도 성학의 명칭을 사용한 저술로써 위기지학을 체계화하였지만 그것들이 입문의 방법을 제시하는 점에서는 과거 선유들의 언급을 되풀이하는 것과도 다르다. 여헌은 그가 터득한 역(易)의 이치와 그것을 다루는 역학(易學)의 이치(易簡)를 매우 간결하게 요약하였고, 동시에 천지의 도에 두루 통할 수 있도록 인간사의 다양한 과정과 변수들을 통괄하여 섭렵하고 통달할 수 있는 공부의 과정을 설계한 것이라고 판단된다. 그러한 의미에서 여헌의 "역학도설"이 위기지학의 전개과정에서 중국의 전통을 잘 발전시켜서 완결된 체계를 구성했다고 평가된다.

• 한국철학논집
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• 제38호
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• pp.63-96
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• 2013

주희 장식과 더불어 동남삼현(東南三賢)이라 일컬어지는 여조겸(呂祖謙)은 남송시대 대표적인 철학자 가운데 한 사람이다. 여조겸은 심후한 가학(家學)을 바탕으로 당시의 학자들과 폭넓게 교류하며 자신의 학문체계를 건립하였다. 여조겸의 심론(心論)은 기본적으로 맹자의 양심(良心) 본심(本心)에 관한 이론에 기초하고 있다. 여조겸은 초심(初心) 내심(內心)을 통해 이러한 양심의 의미를 설명하였다. 여조겸에 따르면 양심이 외부사물과 접촉했을 때 바로 생기는 마음이 초심(初心)이며, 초심(初心)은 외부사물에 대해 옳은 판단을 내릴 수 있다. 내심(內心)은 도덕적 자각능력을 회복한 양심 본심을 의미한다. 여조겸의 심론(心論)의 중요한 특징은 심외무도(心外無道) 심외무천(心外無天)에 있다. 여조겸은 이를 통해 마음과 천(天) 도(道) 리(理)가 하나임을 강조하였다. 여조겸은 아호사(鵝湖寺)의 모임을 주선하며, 주희와 육구연의 학문적 절충을 위해 노력하였다. 그러나 발명본심(發明本心)을 주장했던 육구연과는 달리 양심의 자각과 더불어 도문학(道問學) 역시 중시하였다. 한편 여조겸은 주희와 마찬가지로 경(敬)을 중시하였다. 그러나 여조겸에게 있어 경(敬)이란 순일불잡(純一不雜)한 도덕심(道德心)의 무간단(無間斷)을 의미하며, 성(誠)과 동일한 함의를 지니고 있다. 여조겸은 독서와 강학을 중시하였지만, 마음의 자각과 반성, 간단(間斷) 없는 지속을 통해 리(理)를 이해하고 실현할 수 있다고 주장하였다. 이와 같이 주희와의 절충과 대립을 통해 드러나는 여조겸의 심학적(心學的) 학풍(學風)은 남송 시대 사상계에서 중요한 의미를 지닌다고 할 수 있다.

• Asian Pacific Journal of Cancer Prevention
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• 제13권11호
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• pp.5653-5657
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• 2012

Objectives: To investigate the effect of glycopeptide-preferring polypeptide GalNAc transferase 1 (ppGalNAc T1 ) targeted RNA interference (RNAi) on the growth and migration of human bladder carcinoma EJ cells in vitro and in vivo. Methods: DNA microarray assays were performed to determine ppGalNAc Ts(ppGalNAc T1-9) expression in human bladder cancer and normal bladder tissues. We transfected the EJ bladder cancer cell line with well-designed ppGalNAc T1 siRNA. Boyden chamber and Wound healing assays were used to investigate changes of shppGalNAc T1-EJ cell migration. Proliferation of shppGalNAc T1-EJ cells in vitro was assessed using [3H]-thymidine incorporation assay and soft agar colony formation assays. Subcutaneous bladder tumors in BALB/c nude mice were induced by inoculation of shppGalNAc T1-EJ cells and after inoculation diameters of tumors were measured every 5 days to determine gross tumor volumes. Results: ppGalNAc T1 mRNA in bladder cancer tissues was 11.2-fold higher than in normal bladder tissues. When ppGalNAc T1 expression in EJ cells was knocked down through transfection by pSUPER-shppGalNAc T1 vector, markedly reduced incorporation of [3H]-thymidine into DNA of EJ cells was observed at all time points compared with the empty vector transfected control cells. However, ppGalNAc T1 knockdown did not significantly inhibited cell migration (only 12.3%). Silenced ppGalNAc T1 expression significantly inhibited subcutaneous tumor growth compared with the control groups injected with empty vector transfected control cells. At the end of observation course (40 days), the inhibitory rate of cancerous growth for ppGalNAc T1 knockdown was 52.5%. Conclusion: ppGalNAc T1 might be a potential novel marker for human bladder cancer. Although ppGalNAc T1 knockdown caused no remarkable change in cell migration, silenced expression significantly inhibited proliferation and tumor growth of the bladder cancer EJ cell line.

• Asian Pacific Journal of Cancer Prevention
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• 제13권11호
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• pp.5777-5783
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• 2012

Published data on the associations between tumor necrosis factor-alpha (TNF-${\alpha}$) promoter -308G>A and -238G>A polymorphisms and cervical cancer risk are inconclusive. To derive a more precise estimation of the relationship, a meta-analysis was performed. Data were collected from MEDLINE and PubMed databases. Crude odds ratios (ORs) with 95% confidence intervals (CIs) were calculated in a fixed/random effect model. 13 separate studies including 3294 cases and 3468 controls were involved in the meta-analysis. We found no association between TNF-${\alpha}$-308G>A polymorphism and cervical cancer in overall population. In subgroup analysis, significantly elevated risks were found in Caucasian population (A vs. G: OR = 1.43, 95% CI = 1.00-2.03; AA vs. GG: OR = 2.09, 95% CI = 1.34-3.25; Recessive model: OR = 2.09, 95% CI = 1.35-3.25) and African population (GA vs. GG: OR = 1.53, 95% CI = 1.02-2.30). An association of TNF-${\alpha}$-238G>A polymorphism with cervical cancer was found (A vs. G: OR = 0.61, 95% CI = 0.47-0.78; GA vs. GG: OR = 0.59, 95% CI = 0.45-0.77; Dominant model: OR = 0.59, 95% CI = 0.46-0.77). When stratified by ethnicity, similar association was observed in Caucasian population (A vs. G: OR = 0.62, 95% CI = 0.46-0.84; GA vs. GG: OR = 0.59, 95% CI = 0.43-0.82; Dominant model: OR = 0.60, 95% CI = 0.44-0.83). In summary, this meta-analysis suggests that TNF-${\alpha}$-238A allele significantly decreased the cervical cancer risk, and the TNF-${\alpha}$-308G>A polymorphism is associated with the susceptibility to cervical cancer in Caucasian and African population.

• Asian Pacific Journal of Cancer Prevention
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• 제14권6호
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• pp.3877-3880
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• 2013

Objective: To explore the expression and significance of tumor specific growth factor (TSGF), carcinoembryonic antigen (CEA) and alpha fetoprotein (AFP) in cancer tissue and serum of patients with colon cancer. Materials and Methods: Radical surgery for colon cancer was performed on 43 patients with laparoscopu under conditions of general anesthesia. The Elisa method was used to detect the levels of serum TSGF, CEA and AFP before and after radical operation, and cancer tissue underwent TSGF, CEA and AFP immunohistochemistry staining after laparoscopic surgery. The decreased conditions of serum TSGF, CEA and AFP in patients with colon cancer at different levels of differentiation and clinical stagings were analyzed, and the relationships of expression rates between histological types, colon cancer morphology, lymph node metastasis and TSGF, CEA as well as AFP in cancer tissue were assessed. Results: Compared with before radical surgery, the levels of serum TSGF, CEA and AFP decreased notably in patients after operations (p<0.01). The decreased degree of TSGF and CEA was the largest in patients with poorly differentiated cancer tissue (p<0.01), while that of AFP was noted in patients with moderately differentiated cancer tissue (p<0.01). The decreased degree of TSGF and AFP was the largest in patients at phase Dukes A (p<0.01), while that of CEA in patients at phase Dukes C (p<0.01). There were no significant differences among the positive expression rates of TSGF, CEA and AFP with different histological types and colon cancer morphologies (p>0.05). The positive expression rates of TSGF and CEA in patients with lymph node metastasis were significantly higher than those without lymph node metastasis (p<0.01). Conclusions: TSGF, CEA and AFP can be used to evaluate the effect of radical operation for colon cancer, and the changed levels of different markers are associated with tumor differentiation, clinical stating and presence or absence of lymph node metastasis.

• 대한한의학회지
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• 제25권2호
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• pp.194-206
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• 2004

Objectives : JiaoTeng-Yuan(교등원) is said to be a prescription for preservation of health in $\ulcorner$HuaTuo.ZhongZangJing (화타.중장경)$\lrcorner$. It is known to have the effect of Bu-Shen (보신: strengthening kidney) and Yi-Shou (익수: prolonging the span of one's life). This study investigates whether JTY is effective on inhibition of oxidation stress. Methods : Sprague-Dawley rats (12-week-old, weight $300{\pm}20g$) were divided into 3 groups. The normal group (n=8) was injected with PBS (1ml/body, s.c) subcutaneously behind the neck. The control group (n=8) was injected with D-galactose (50mg/kg, 1ml PBS/body, s.c) to induce pathological animals. The JTY group was injected with the same treatment as the control group, and fed containing JTY (10%). All groups were treated 1 time per day for 6 weeks. After the rats were sacrificed anti-oxidant enzyme (SOD, CAT, G-px) activity, GSH quantity of RBC and tissue (heart, liver and kidney), and plasma Vit-C quantity were examined. Also, the MDA levels of liver and kidney, lipofuscin of heart and endurance of erythrocyte membrane were measured. Results : In the JTY group, RBC's SOD activity decline was halted by 21 % of the normal level, compared to the control group, G-px activity (unit/g of Hb) increased significantly, compared to the normal group, and the level of Vit-C in plasma increased by 16%. Heart's SOD activity was kept at the same level as that of the normal group; and CAT activity decline was halted by 26%. Kidney's CAT and G-px activities were kept at the same level as that shown in the normal group, implying the existence of a halting effect. Liver also showed a slight halting effect against the decline of anti-oxidant ability, but the effect was not significant (${\alpha}=0.05$). A comparison between the levels of peroxide in SD rats showed that the level of TBARS in plasma increased significantly in the control group and that it was normal in the JTY group. The livers in the JTY group, compared to those in the control group, showed 36% halting effect of the normal level while their kidney's indicated the level significantly lower than the normal level. Heart's lipofuscin increased significantly in the control group, but was alike in both the JTY and the normal groups. Endurance of erythrocyte membrane (%) decreased significantly in the control group while it was kept at the similar level in both the JTY and the normal groups, indicating the halting effect. Conclusions : This study suggests that JTY is effective to defend oxidation stress caused by D-galactose in the animals. It showed that the anti-oxidant ability was maintained and strengthened. On the other hand, it reduced the level of peroxide in animals. In sum, JTY appeared to have the equilibrium normal physiological function in SD rat.

• Journal of Microbiology and Biotechnology
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• 제27권3호
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• pp.561-572
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• 2017

The global commercial cultivation of transgenic crops, including glyphosate-tolerant soybean, has increased widely in recent decades with potential impact on the environment. The bulk of previous studies showed different results on the effects of the release of transgenic plants on the soil microbial community, especially rhizosphere bacteria. In this study, comparative analyses of the bacterial communities in the rhizosphere soils and surrounding soils were performed between the glyphosate-tolerant soybean line NZL06-698 (or simply N698), containing a glyphosate-insensitive EPSPS gene, and its control cultivar Mengdou12 (or simply MD12), by a 16S ribosomal RNA gene (16S rDNA) amplicon sequencing-based Illumina MiSeq platform. No statistically significant difference was found in the overall alpha diversity of the rhizosphere bacterial communities, although the species richness and evenness of the bacteria increased in the rhizosphere of N698 compared with that of MD12. Some influence on phylogenetic diversity of the rhizosphere bacterial communities was found between N698 and MD12 by beta diversity analysis based on weighted UniFrac distance. Furthermore, the relative abundances of part rhizosphere bacterial phyla and genera, which included some nitrogen-fixing bacteria, were significantly different between N698 and MD12. Our present results indicate some impact of the glyphosate-tolerant soybean line N698 on the phylogenetic diversity of rhizosphere bacterial communities together with a significant difference in the relative abundances of part rhizosphere bacteria at different classification levels as compared with its control cultivar MD12, when a comparative analysis of surrounding soils between N698 and MD12 was used as a systematic contrast study.

• The Korean Journal of Physiology and Pharmacology
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• 제22권6호
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• pp.607-616
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• 2018

The effect of melatonin on juveniles with cardio fibrosis is poorly understood. We investigated whether HDACs participate in the anti-fibrotic processes regulated by melatonin during hypertrophic remodeling. Abdominal aortic constriction (AAC) was employed in juvenile rats resulting in pressure overload-induced ventricular hypertrophy and melatonin was subsequently decreased via continuous light exposure for 5 weeks after surgery. AAC rats displayed an increased cross-sectional area of myocardial fibers and significantly elevated collagen deposition compared to sham-operated rats, as measured by HE and Masson Trichrome staining. Continuous light exposure following surgery exacerbated the increase in the cross-sectional area of myocardial fibers. The expression of HDAC1, HDAC2, HDAC3, HDAC4 and HDAC6 genes were all significantly enhanced in AAC rats with light exposure relative to the other rats. Moreover, the protein level of $TNF-{\alpha}$ was also upregulated in the AAC light exposure groups when compared with the sham. However, Smad4 protein expression was unchanged in the juveniles' hearts. In contrast, beginning 5 weeks after the operation, the AAC rats were treated with melatonin (10 mg/kg, intraperitoneal injection every evening) or vehicle 4 weeks, and sham rats were given vehicle. The changes in the histological measures of cardio fibrosis and the gene expressions of HDAC1, HDAC2, HDAC3, HDAC4 and HDAC6 were attenuated by melatonin administration. The results reveal that melatonin plays a role in the development of cardio fibrosis and the expression of HDAC1, HDAC2, HDAC3, HDAC4 and HDAC6 in cardiomyocytes.