• Horticultural Science & Technology
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• v.31 no.6
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• pp.813-820
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• 2013

Monoterpenoids were recently found as main biologically active compounds which is responsible for various physiological effect in goat's beard (Aruncus dioicus). Ethyl acetate extract of A. dioicus (ADE) was treated to B16F10 melanoma cells for the examination of whitening activity. MTT assay was performed to evaluate cell toxicity and the result showed that slight cell toxicity (> 10%) by over $500{\mu}g{\cdot}mL^{-1}$. Thus, 0, 5, 10, or $50{\mu}g{\cdot}mL^{-1}$ ADE was used for further experiments. We found that tyrosinase activity was decreased according to ADE concentration, and the total melanin content was also dramatically reduced. Especially with $50{\mu}g{\cdot}mL^{-1}$ ADE treatment tyrosinase activity was reduced to 35.6%, and 58.8% of melanin content was lowered. In addition, whitening related proteins including tyrosinase, tyrosinase related protein 1 (TRP1), TRP2, microphthalmia associated transcription factor (MITF) and cAMP and protein kinase A (PKA) were reduced by ADE treatment. It caused decreased phosphorylation of cAMP response binding protein (CREB) but increased phosphorylation of extracellular signal related kinase (ERK). Therefore, in this paper we would like to suggest the potent usage of A. dioicus natively grown in Ulleungdo, Korea as materials of functional cosmetics by confirming whitening activity related with melanin content.

• Korean journal of food and cookery science
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• v.20 no.2
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• pp.180-186
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• 2004

Evidence shows there are flavonoid and polyphenol compounds in Ulmus cortex which has excellent antioxidant, anticancer and antimicrobial properties. This study summarizes our findings on effects of Ulmus cortex extracts(UDE) on quality characteristics of bread and the changes of microbial counts during storage. Dough yield and specific loaf volume of bread decreased significantly because the amount of UDE increased. Lightness of bread surface, redness of crumb and yellowness of crust were significantly increased. Texture evaluation showed that hardness was the highest in the bread prepared with 30% UDE. Sensory evaluation showed that odor, taste, texture, and overall acceptability were the best in bread which was made with 10% UDE. At the beginning of the period of storage, there were not significant differences of microbial cell count as increasing UDE, but significant decreasing was observed after passing 6 days. A negative correlation was generally observed between the sensory and mechanical properties. The results of total microbial count show that adding UDE in processing bread extend bread's storage time.

• Korean Journal of Food Science and Technology
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• v.30 no.5
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• pp.1209-1213
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• 1998

The gamma-radiation sensitivity of four kinds of Escherichia coli O157:H7 was investigated in frozen cells $(-18^{\circ}C)$ with 0.1 M phosphate buffer and inoculated cells in beef. The maximum populations were observed at 12 hr when E. coli O157:H7 was incubated in the tryptic soy broth at $37^{\circ}C$. In the case of the frozen cells at logarithmic phase, the $D_{10}$ and $12D_{10}$ values of four kinds of E. coli O157:H7 were $0.09{\sim}0.15\;kGy$ and $1.08{\sim}1.80\;kGy$, and inactivation factors were $13.33{\sim}22.22$ and $20.00{\sim}33.33$ at radiation doses of 2 and 3 kGy, respectively. The radiosensitivity of inoculated E. coli O157:H7 in beef showed the $D_{10}$ value of $0.30{\sim}0.47\;kGy$, the $12D_{10}$ value of $3.60{\sim}5.64\;kGy$, and inactivation factor of $4.26{\sim}10.00$. The radiosensitivity of the frozen cells was higher than that of the inoculated E. coli O157:H7 in beef. Gamma irradiation at doses within the range of 1.5 to 3 kGy is considered to be an effective method to control E. coli O157:H7 in beef.

• Applied Biological Chemistry
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• v.40 no.3
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• pp.189-195
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• 1997

The structural basis of Iysine specificity of Achromobacter protease I (API) was investigated by means of site-directed mutagenesis. The precursor protein in which Glu190, one of the two candidates for determining Iysine specificity, was substituted by glutamine, aspartic acid or leucine was processed autocatalytically to attaln full pretense activity with lysine specificity. The substitution of the other candidate, Asp225, for asparagine or leucine produced no mature active forms of pro-API. The precursor protein of the mutant D225E slowly matured autocatalytically. The lysylendopeptidase activity of the mature D225E was 0.25% of that of native API, and this reduced activity is mainly due to a decrease in the affinity of the enzyme for lysine. These results suggest that Asp225 plays a critical rol in restricted substrate specificity as a lysylendopeptidase. However, D225E exhibited no measurable activity for synthetic ornithine substrate. Since the hydroxyl group of Ser194 in this mutant retained essentially the same reactivity to DFP or PMSF as that in native API, it can be noted that a methylene unit longer side chain of residue 225 is not compensated by a methylene unit shorter side chain at subsite P1 in the bound substrate.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.1
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• pp.13-19
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• 1996

This study was conducted to establish the rapid determination method for major components of maturing covered barley grains, and to improve the efficiency of selection in barley breeding. Near Infrared Reflectance Spectroscopy (NIRS) is an established, economical and nondestructive technique applied widely to the food and feed industry. 34 barley lines were sampled at 5 day-interval from 25 to 35 days after heading. A standard regression analysis for the data obtained by analytical laboratory methods and NIRS method was carried out to get a useful calibration equation. The simple significant correlation between these two methods at 25 days after heading was recognized in starch and $\beta$-glucan contents. At 30 days after heading the data obtained by two methods showed significant correlation in starch, $\beta$-glucan and protein contents. Analyzed data and that from NIRS method at 35 days after heading was significantly correlated in starch and protein contents. It was concluded that the applicability of NIRS method for the components analysis in maturing barley grains was different depending on maturing stages and components.

• International Journal of Industrial Entomology and Biomaterials
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• v.21 no.2
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• pp.180-183
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• 2010

This study was performed to investigate the temperature-dependent development, longevity and oviposition of an indigenous larval parasitoid, Meteorus pulchricornis, on tobacco cutworm, Spodoptera litura. M. pulchricornis were reared at nine constant temperatures between 15 and $35^{\circ}C$. The developmental times of each three developmental stage decreased from 38.7 to 16.3 d between 15 and $30^{\circ}C$. However, M. pulchricornis showed longer developmental time at $32.5^{\circ}C$ (9.5, 7.7 and 17.2 days for each three developmental stage) than at $30^{\circ}C$ (8.9, 7.3 and 16.3 days for each three developmental stage). Immature M. pulchricornis could not develop any more at $35^{\circ}C$. The lower developmental threshold estimated by linear regression equation for the egg to cocoon, cocoon to adult emergence and egg to adult emergence were 5.1, 4.6 and $4.5^{\circ}C$. The thermal constant for each of the three stages were 217.2, 176.2 and 403.8 degree-days, respectively. When no food or 50% honey solution as a food source is provided for M. pulchricornis, the parasitoid survived for 8.3 and 55.9 days at $25^{\circ}C$, respectively. M. pulchricornis females laid 5.2 eggs daily and total of 131.6 eggs at $25^{\circ}C$ until it died. Peak age-specific fecundity was observed on $14^{th}$ day (9.6 cocoons) after parasitoid emergence and gradually decreased thereafter.

• Journal of Chest Surgery
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• v.33 no.8
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• pp.648-654
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• 2000

Background: Cardiopulmonary bypass during open heart surgery causes systemic inflammatory respose. IL-10 is an anti-inflammatory cytokine that inhibits inflammatory process and protects organ function by down regulation of pro-inflammatory cytokine release and maintenance of blood level balance with pro-inflammatory cytokines. Mateial and Method: Plasma IL-10 levels were measured and analyzed in 22 patients who underwent open heart surgery (11 cases of coronary artery bypass graft, 11 cases of valve replacement) under cardiopulmonary bypass since 1988 January to July at Department of Thoracic and Czardiovascular surgery, Yeungnam University Hospital. 1g of methylprednisolone was administrated to thirteen patients randomly. Blood samp.es were taken and collected at the time of induction of anesthesia, 10 min before cardiopulmonary bypass, 10 min after starting of CPB, 10 min aftr aortic cross clamping, 10 min after ACC release, and 10 min, 2 hours, and `5 hours after CPB respectively. The plasma levels of IL-10 were determined by enzyme-linked immunosorbent assays(ELISA). Wilcoxon-Raule Sum test was used for statistical analysis. Result: In all 22 patients, cardiopulmonary bypass time was used for statistical analysis. Result: In all 22 patients, cardiopulmonary bypass time was 171$\pm$41.4 min and aortic cross clamp time was 118$\pm$36.5 min. Peak IL-10 level was achieved at 10 min after ACC(361.0$\pm$52.81pg/ml) and was decreased sharply at 2 hours after CPB. Peak IL-10 level was correlated positively with aortic cross clamp time(p=0.011); however, it did not correlated with bypass time(p=0.181). In valve replacement group, mean IL-10 level at peak point was 567.89$\pm$107.69 pg/ml and was significantly higher than that of coronary artery bypass group(205.67$\pm$192.70 pg/ml)(p<0.001). ACC time in valve replacement group was significantly longer than that of coronary artery bypass group(p<0.01), however, bypass time was not(p=0.212). Thirteen patients with steroid pretreatment before starting of CPB showed relatively higher plasma IL-10 level than in control group, however, no statistical significance was noted(p=0.19). Conclusion: plasma level of IL-10 was increased in association with cardiopulmonary bypass and revealed peak at 10 min after ACC release. IL-10 level was correlated positively with ACC time. Therefore, systemic inflammatory respeonse in association with cardiopulmonary bypass could be decreased by reducing ACC time during cardiac surgery.

• Journal of Korea Water Resources Association
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• v.34 no.4
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• pp.303-316
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• 2001

In this study, Radial Basis Function(RBF) Neural Networks Model, a kind of Hybrid Neural Networks was applied to hydrological forecasting in a small watershed. RBF Neural Networks Model has four kinds of parameters in it and consists of unsupervised and supervised training patterns. And Gaussian Kernel Function(GKF) was used among many kinds of Radial Basis Functions(RBFs). K-Means clustering algorithm was applied to optimize centers and widths which ate the parameters of GKF. The parameters of RBF Neural Networks Model such as centers, widths weights and biases were determined by the training procedures of RBF Neural Networks Model. And, with these parameters the validation procedures of RBF Neural Networks Model were carried out. RBF Neural Networks Model was applied to Wi-Stream basin which is one of the IHP Representative basins in South Korea. 10 rainfall events were selected for training and validation of RBF Neural Networks Model. The results of RBF Neural Networks Model were compared with those of Elman Neural Networks(ENN) Model. ENN Model is composed of One Step Secant BackPropagation(OSSBP) and Resilient BackPropagation(RBP) algorithms. RBF Neural Networks shows better results than ENN Model. RBF Neural Networks Model spent less time for the training of model and can be easily used by the hydrologists with little background knowledge of RBF Neural Networks Model.

• Applied Chemistry for Engineering
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• v.17 no.6
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• pp.638-643
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• 2006

Pyrolytic reaction study of dichloromethane ($CH_{2}Cl_{2}$) in excess hydrogen was performed to investigate pyrolytic reaction pathways at a pressure of 1 atm with residence times of 0.3~2.0 sec in the temperature range of $525{\sim}900^{\circ}C$. A constant feed molar ratio $CH_{2}Cl_{2}$:$H_{2}$ of 4:96 was maintained through the experiment. Reagent loss and product formation were monitored by using an on-line gas chromatograph, where batch samples were analyzed by GC/MS. Complete destruction(99%) of the parent reagent was observed at temperature near $780^{\circ}C$ with residence time over 1 sec. Major products observed were $CH_{3}Cl$, $CH_{4}$, $C_{2}H_{4}$, $C_{2}H_{6}$, and HCl. Minor products included $CHClCCl_{2}$, CHClCHCl, $CH_{2}CHCl$, and $C_{2}H_{2}$. The pyrolytic reaction pathways to describe the important features of intermediate product distributions and reagent loss, based upon thermodynamic and kinetic principles, were suggested. The results of this work provided a better understanding of pyrolytic decomposition processes which occur during the pyrolysis of $CH_{2}Cl_{2}$ and similar chlorinated methanes.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.8
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• pp.5062-5069
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• 2014

In this study, professional oral care was provided and 3 times a day twice a week by a dental hygienist for 3 months from July 24 to October 25, 2011, to 43 long-term patients in a single nursing facility located at the city of D, and the number of mutans strepococci colonies within the saliva and the level of salivation were then analyzed after 3 samplings with an interval of 1 month to compare the intraoral conditions among the subjects and examine the effects of professional oral care on the Streptococcus mutans population in the intraoral region of long-term patients in a nursing facility. The level of salivation was elevated from 5.8 ml to 6.4 ml after one month and to 7.5mL after two months. The population of bacteria decreased from $6{\times}10^8CFU/ml$ to $3{\times}105CFU/ml$. In terms of the change in the bacterial population in accordance with systemic diseases, the patients with hypertension and diabetes showed a meaningful decrease in the population. As more dental charge and prosthesis are present, the number of bacteria decreased significantly. On account of the close relationship between a professional oral care and the population of Streptococcus mutans at the intraoral region of long-term patients in nursing facilities, various dental health programs should be researched and developed to consider the characteristics of long-term patients in nursing facilities and manage them continuously but effectively.