• Title/Summary/Keyword: Ye-Kim

Search Result 3,153, Processing Time 0.032 seconds

Optimal Production Conditions of Streptomyces griseus Trypsin (SGT) in Streptomyces lividans

  • Koo, Bon-Joon;Kim, Joung-Mee;Byun, Si-Myong;Hong, Soon-Kwang
    • BMB Reports
    • /
    • v.32 no.1
    • /
    • pp.86-91
    • /
    • 1999

  • The sprT gene encoding Streptomyces griseus trypsin (SGT) was introduced into Streptomyces lividans TK24 and Streptomyces lividans 1326 to study which strain would be better to overexpress the extracellular proteinase. Various media with different compositions were also used to maximize the productivity of SGT in heterologous hosts. The SGT productivity was best when the transformants of S. lividans TK24 and 1326 were cultivated in R2YE medium, and their relative trypsin activity of the culture broth measured with an artificial chromogenic substrate, N-${\alpha}$-benzoyl-DL-arginine-${\rho}$-nitroanilide, were 382 units/ml and 221 units/ml, respectively. They produced high levels of SGT in GYE medium but relatively lower than those in R2YE medium, and negligible amount of SGT was produced in Ferm, RASF, LIVID, and NDSK media. Considering non-SGT associated activity in Pronase powder, it was estimated that the transformant of S. lividans TK24 can produce SGT in R2YE 3.5 times more than the amount by S. griseus 10137 from which the sprT gene had been originated. The growth of S. lividans reached the maximum level of cell mass at 5 d of culture, but SGT production started in the stationary phase of cell growth and kept increasing until the ninth day of culture in R2YE medium, but in GYE media the productivity reached at the maximum level at 7 d of cultivation.

  • PDF

치질은 고칠수 있나

  • Kim, Ye-Heum
    • 건강소식
    • /
    • v.3 no.5 s.20
    • /
    • pp.24-27
    • /
    • 1975

  • 이른바「질」자가 붙는 병치고 잘 낫는 병이 없다. 그렇다고 죽지도 않는다. 병의 형태도 가지가지 이고, 치료법도 가지가지다-치질도 그런 병의 한 가지. 그러면 치질은 왜 생기며, 완전하게 고칠 수도 있을가.

  • PDF