• Title/Summary/Keyword: Ye-Kim

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Overproduction of Bacterial Trypsin in Streptomyces - Optimization for Streptomyces griseus Trypsin Production by Recombinant Streptomyces (미생물을 이용한 트립신 과대 생산 연구 - Streptomyces용 숙주-벡터계를 이용한 트립신 유전자의 대량발현 최적화 -)

  • Kim, Jong-Hee;Hong, Soon-Kwang
    • Microbiology and Biotechnology Letters
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    • v.36 no.1
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    • pp.28-33
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    • 2008
  • The expression vector (pWHM3-TR1R2) for sprT gene encoding Streptomyces griseus trypsin (SGT) followed by two regulatory genes, sgtR1 and sgtR2, was introduced into Streptomyces lividans TK24 and Streptomyces griseus IFO 13350. Various media with different compositions were used to maximize the productivity of SGT in the recombinant trains. he SGT productivity was best when the transformant of S. lividans TK24 was cultivated in R2YE medium (0.74 unit/mL) at 5 days of cultivation. C5/L (0.66 unit/mL) medium also gave a good productivity, but Livid (0.08 unit/mL) and NDSK (0.06 unit/mL) yielded poor productivities. S. griseus IFO 13350/pWHM3-TR1R2 produced SGT by 1.518 unit/mL (C5/L), 1.284unit/mL (R2YE),0.932 unit/mL (NDSK), and 0.295 unit/mL (Livid) at 7 days of cultivation, which was much higher than those from S. lividans TK24/TR1R2. The SGT protein was purified from the culture broth of S. griseus IFO 13350/pWHM3-TR1R2 in C5/L to homogeneity via ammonium sulfate fractionation, and CM-sepharose and SP-sepharose column chromatographies. The specific activity of purified SGT was 69,252 unit/mg, and the final purification fold and recovery yield were 6.5 and 1.4%, respectively.

Improvement and Systematization of Pre-Study Work for Design Value Engineering in Construction Projects by Quality Function Deployment (품질기능전개(QFD) 기법을 적용한 건설프로젝트 설계VE 준비단계 업무 개선 및 체계화)

  • Yang Jin Kook;Kim Soo-Yong
    • Korean Journal of Construction Engineering and Management
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    • v.6 no.4 s.26
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    • pp.122-132
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    • 2005
  • The importance of design value engineering in construction projects recently is rising, which deal about in reduction of cost and improvement of quality. Especially, From half of this year 2005, It is predicted that the value engineering is necessary to be carried out in the projects having project cost more than 10 billion won. In increasing importance of value engineering, we require a way by which we accomplish value engineering systemically and effectively though it is not common in domestic situation. So, this case study shows a way in carrying out value engineering effectively. As the way is to apply QFD(Quality Function Deployment) in pre-study work of design value engineering, this is expected to contribute as function analysis and reflect the requirement of users or owners.

Pill Identification System Using Image Processing in Smart Phone (스마트 폰의 영상 처리를 통한 알약 인식 시스템)

  • Hwang, Jin-sung;Kim, Kyung-yeon;Jeon, Ye-rin;Choi, Ye-rim;Park, Kwang-Hyun
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2013.10a
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    • pp.1010-1011
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    • 2013
  • In this paper, we have developed a system for identifying pills and providing their information by image processing to assist the health care of the elderly. A wide variety of pills can lead to the difficulty of distinguishing for the elderly at home and results in unusage and abandonment of the pills. To resolve this problem, we obtain images from a camera of a smart phone, extract features such as shape, line and color by image processing, classify pills, and provide information including the name, effect and directions for the usage of the pills.

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Detection of Needle in trimmings or meat offals using DCGAN (DCGAN을 이용한 잡육에서의 바늘 검출)

  • Jang, Won-Jae;Cha, Yun-Seok;Keum, Ye-Eun;Lee, Ye-Jin;Kim, Jeong-Do
    • Journal of Sensor Science and Technology
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    • v.30 no.5
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    • pp.300-308
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    • 2021
  • Usually, during slaughter, the meat is divided into large chunks by part after deboning. The meat chunks are inspected for the presence of needles with an X-ray scanner. Although needles in the meat chunks are easily detectable, they can also be found in trimmings and meat offals, where meat skins, fat chunks, and pieces of meat from different parts get agglomerated. Detection of needles in trimmings and meat offals becomes challenging because of many needle-like patterns that are detected by the X-ray scanner. This problem can be solved by learning the trimmings or meat offals using deep learning. However, it is not easy to collect a large number of learning patterns in trimmings or meat offals. In this study, we demonstrate the use of deep convolutional generative adversarial network (DCGAN) to create fake images of trimmings or meat offals and train them using a convolution neural network (CNN).

The effects of Nintendo games in the face of COVID-19 pandemic. (코로나19 팬데믹시대, 닌텐도 게임의 영향력에 대한 연구)

  • Kim, Mi Kyoung;Kang, Hyosoon
    • Journal of Korea Game Society
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    • v.21 no.2
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    • pp.33-42
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    • 2021
  • Despite people's expectation, COVID-19 has turned out to be a burden people have to carry for a while. The age of "Untact" has come. While many outdoor activites have been restricted, it is crucial to find other ways to maintain physical/mental health ideally indoors. As a mediative gesture, people are now taking notice of 'Console games', through which they can party, travel, and exercise in virtual reality even harder than in real life. In thesis, we will examine Nintendo Switch Consoles in relation to direction Korean game industry has to take to maintain people's physical/mental health.

The couple of netrin-1/α-Synuclein regulates the survival of dopaminergic neurons via α-Synuclein disaggregation

  • Eun Ji Kang;Seung Min Jang;Ye Ji Lee;Ye Ji Jeong;You Jin Kim;Seong Su Kang;Eun Hee Ahn
    • BMB Reports
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    • v.56 no.2
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    • pp.126-131
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    • 2023
  • The abnormal accumulation and aggregation of the misfolded α-synuclein protein is the neuropathological hallmark of all α-synucleinopathies, including Parkinson's disease. The secreted proteins known as netrins (netrin-1, netrin-3, and netrin-4) are related to laminin and have a role in the molecular pathway for axon guidance and cell survival. Interestingly, only netrin-1 is significantly expressed in the substantia nigra (SN) of healthy adult brains and its expression inversely correlates with that of α-synuclein, which prompted us to look into the role of α-synuclein and netrin-1 molecular interaction in the future of dopaminergic neurons. Here, we showed that netrin-1 and α-synuclein directly interacted in pre-formed fibrils (PFFs) generation test, real time binding assay, and co-immunoprecipitation with neurotoxin treated cell lysates. Netrin-1 deficiency appeared to activate the dopaminergic neuronal cell death signal pathway via α-synuclein aggregation and hyperphosphorylation of α-synuclein S129. Taken together, netrin-1 can be a promising therapeutic molecule in Parkinson's disease.

Accuracy Analysis and Comparison in Indoor Localization Utilizing Bluetooth Beacon and UWB (블루투스 비콘과 UWB의 실내측위 정확도 비교 및 분석)

  • Byun, Seok-Ju;Yoo, Ji-Hyeon;Kim, Ye-Bin;Park, Yang-Bae;Lee, Ye Hoon
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • fall
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    • pp.183-186
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    • 2021
  • 현재 IoT의 발달로 인해서 많은 위치기반 서비스들이 개발되고 있고 이러한 서비스들을 위해서는 사용자의 정확한 위치 획득이 요구되고 있다. 현재 실내 환경에서 사용자의 위치 획득을 위한 여러 장치들 중 블루투스 비콘이 널리 사용되고 있는데, 비콘은 저전력으로 수명이 길고 설치하기 용이한 장점이 있지만 비콘으로부터 수신되는 신호 자체의 불안정성과 수신신호에 여러 잡음들이 섞이는 문제점 때문에 측위 결과에 큰 오차가 발생하게 되는 단점도 있다. 한편, 최근에는 보다 원할한 위치기반 서비스 제공을 위하여 UWB 기능이 스마트폰에 내재되어 나오면서 사용자에게 점차 보급되는 추세이다. UWB는 블루투스 비콘에 비해 가격이 비싸고 전력소모가 많지만 측위에서의 높은 정확도를 얻을 수 있다고 알려져 있다. 본 논문에서는 비콘과 UWB의 두 방식을 이용하여 실내측위를 수행할 때 실제 측정을 통해서 실내 전파수신 환경을 분석하고, 측정된 데이터를 바탕으로 실내 전파수신 환경을 모델링하여 시뮬레이션을 수행하였다. 측정 데이터와 시뮬레이션 결과의 정확도를 상호 분석하였으며, 블루투스 비콘과 UWB 방식의 실내측위 수행결과를 측정과 시뮬레이션 결과를 바탕으로 비교, 분석하였다.

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