• 제목/요약/키워드: Ye-Kim

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Dedicated Delivery Platform Based on the User Experience of the Visually Impaired (시각장애인 사용자 경험에 기반한 전용 배달 플랫폼 연구 및 개발)

  • Kim, Ye-Won;Kim, Yun-Ji;Shin, Ye-Jin;Lee, Jae-Hun;Choi, Si-Eun
    • Annual Conference of KIPS
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    • 2022.11a
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    • pp.974-976
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    • 2022

  • 코로나19 확산 이후 온라인 주문 배달 음식 시장 규모가 커짐에 따라 다양한 음식 배달 모바일 플랫폼이 상용화되고 있다. 기존 배달 서비스의 기능 및 사용자 인터페이스(UI)를 분석한 결과, 비시각장애인의 관점에서만 편리성이 확보되고 있다는 것을 파악했다. 또한 시각장애인을 대상으로 한 인터뷰를 통해, 기존 배달 애플리케이션의 복잡한 UI로 인해 많은 시간이 소요된다는 문제점을 알 수 있었다. 이에 본 연구에서는 시각장애인 대상의 인터뷰와 관찰을 기반으로 사용자의 pain point를 극복할 수 있는 사용자 경험 중심의 배달 플랫폼을 개발하고자 한다. 서비스 이용 과정의 간소화, 시각 장애인을 위한 맞춤형 UI 제공, 접근성 강화를 통해 플랫폼 구성을 차별화한다. 이를 통해 시각장애인 사용자의 배달 플랫폼 이용을 수월하게 하고 장애인의 편리한 생활을 보장하기 위한 서비스들이 향후 많이 개발될 수 있도록 동기를 부여한다. 또한, 본 연구를 확장하여 '시각장애인의 키오스크 사용 관련 이슈 해결 방안'을 향후 연구 주제로 제안한다.

  • https://doi.org/10.3745/PKIPS.y2022m11a.974 인용 PDF

Overproduction of Streptomyces griseus Protease A and B Induces Morphological Changes in Streptomyces lividans

  • Chi, Won-Jae;Kim, Jung-Mee;Choi, Si-Sun;Kang, Dae-Kyung;Hong, Soon-Kwang
    • Journal of Microbiology and Biotechnology
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    • v.11 no.6
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    • pp.1077-1086
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    • 2001

  • The sprA and sprB gene encoding chymotrypsin-like proteases Streptomyces griseus protease A (SGPA) and Streptomyces griseus protease B (SGPB) and the sprT gene that encodes Streptomyces griseus trypsin (SGT) were cloned from Streptomyces griseus ATCC10137 and overexpressed in Streptomyces lividans TK24 as a heterologous host. The chymotrypsin activity of tole culture broth measured with the artificial chromogenic substrate , N-succinyl-ala-ala-pro-phe-p-nitroanilide, was 10, 14 and 14 units/mg in the transformants haboring the sprA, sprB and sprD genes, respectively. The growth of S. lividans reached the maximum cell mass after 4 days of culture, yet SGPA and SGPD production started in the stationary phase of cell growth and kept increasing for up to 10 days of culture in an R2YE medium. The trypsin activity of the culture broth measured with the artificial chromogenic substrate , N-${\alpha}$-benzoyl-DL- arginine-p-nitroanilide , was 16 units/mg and SGT production started in the stationary phase of cell growth and kept increasing for up to 10 days of culture in an R2YE medium. The introduction of the sprA gene into S, lividans TK24 triggered the biosynthesis of pigmented antibiotics, actinorhodin and undecylprodigiosin, and induced significant morphological changes in the colonies in Benedict, R2YE, and R1R2 media. In addition, the introduction of the sprT gene also induced morphological changes in the colony shape without affecting the antibiotic production, thereby implying that certain proteases would appear to play very important and specific roles in secondary-metabolites formation and morphological differentiation in Streptomyces.

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Quotations from the ${\ulcorner}$Linzheng zhinan yian${\lrcorner}$ in the ${\ulcorner}$Wenbing tiaobian${\lrcorner}$: How were they transformed and corrected? ("온병조변"에서 "임증지남의안" 의 온병관련 의안을 변형하고 첨삭한 방법)

  • Kim Han-Sung;Lim Jin Seok;Lee Choong Yeol
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.19 no.4
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    • pp.865-871
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    • 2005

  • The Wenbing tiaobian(온병조변) is the first systematic disease monograph on warm factor disease(온병), written by Wu Jutong(오국통). It was most influenced by the Linzheng zhinan yian(임증지남의안), which was a book of gathering medical case records of Ye tianshi(엽천사). Therefore, there are plenty of quotations from this book in the Wenbing tiaobian. This study investigates the quotations from Linzheng zhinan yian, especially focusing on the way of transformation and correction of the original medical case records on the warm factor disease. The results are as follows: About 104 of 265 provisions in the Wenbing tiaobian were directly quoted from the Linzheng zhinan yian. The provisions quoted from Ye's case records were rearranged according to the categories of the triple burners pattern differentiation(삼초변증) and the causes of warm factor disease. And eve case record was transformed into more general descriptive form in order to put it into the book. For example, the specific figures, and some patients' peculiar symptoms, causes, sex and disease names were omitted. On the other hand, the tongue moss, pulse shape and some symptoms, which were necessary for differentiating patterns, were added. In the case of the formula, some formulas originated from Ye's case records were named newly. And the dose of each herb consisting a formula was determined, and therapeutic principle, taking method and detailed explanation was added to every formula.

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Characterization of Carboxymethylcellulase(CMCase) Produced by Recombinant E. coli Containing CMCase Gene for Cellulomonas sp. YE-5

  • Park, Sung-Won;Her, Nam-Yun;Kim, Dong-Seob;Park, Sun-Jin;Lee, Han-Seung;Park, Hak-Jong;Yu, Ju-Hyun
    • Preventive Nutrition and Food Science
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    • v.2 no.2
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    • pp.174-179
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    • 1997

  • CMCase produced by recombinant E. coli JM109 (pCEH#4) containing CMCase gene from Cellulomonas sp. YE-5 was purified to 24.3 fold and 2.6% yield by ammoniumsulfate precipitation, DEAE-cellulose column chromatography and gel filtration on Sephadex G-100. The optimum pH and temperature for CMCase activity were pH 7.0 and 5$0^{\circ}C$. The enzyme was stable between pH 5.0 and 10.0, and up to 6$0^{\circ}C$. The molecular weight of he enzyme was estimated to be approximately 40,000 daltons by SDS-PAGE. Analysis of the amino acid composition showed that the enzyme contained many glycines and acidic amino acids. The enzyme was an endo-type CMCase and the final enzyme reaction product from hydrolysis of Cm-cellulose by the enzyme was cellobiose. {TEX}$K_{M}${/TEX} value determined with CM-cellulose was 1.28mM.

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