• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.7
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• pp.975-982
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• 2015

Piceatannol (trans-3,4,3',5'-trihydroxystilbene), a natural stilbene, is an analogue of resveratrol. In the present study, possible mechanisms by which piceatannol exerts its pro-apoptotic action in cultured human oral cancer YD-15 cells were investigated. To investigate whether or not piceatannol has effects on cancer cell viability, human oral YD-15 cells were treated with piceatannol (0, 50, and $100{\mu}M$). Piceatannol treatment ($100{\mu}M$) showed the strongest inhibition of cell proliferation and reduced cell viability in a dose-dependent manner. Chromatin condensation detected by DAPI staining significantly increased in a concentration-dependent manner, indicating apoptosis. Piceatannol treatment activated initiator Bax (pro-apoptotic) and cPARP in a concentration-dependent manner. Further, piceatannol induced down-regulation of Bcl-2 (anti-apoptotic). We also evaluated the activity of piceatannol against oral cavity cancer tumors in mice. Piceatannol-treated nude mice bearing YD-15 xenograft tumors exhibited significantly reduced tumor volume and weight due to the potent effect of piceatannol on tumor cell apoptosis, as determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay. Immunohistochemistry staining showed elevated expression of cleaved-caspase-3 as well as reduced expression of Ki-67 in the piceatannol-treated group. Therefore, piceatannol can be developed as a cancer preventive medicine due to its growth inhibitory effects and induction of apoptosis in human oral cancer cells.

• Korean Journal of Social Welfare
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• v.65 no.2
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• pp.79-102
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• 2013

This study aimed at empirically analysing the double discrimination on the elderly with disabilities due to both age and disabilities in the labor market. To achieve this research goal, real employment probabilities and hourly wages of the elderly disabled(below "ED") were compared with those of the young disabled(below "YD") and those of the elderly non-disabled(below "END") to calculate empirical differentials between ED's and YD's, and ED's and END's. The differential decompositions between groups were applied by the Oaxaca(1973). The study results were as followings. First, the real gaps of the employment probabilities between ED and YD only appeared at the regular jobs and the gaps due to the characteristics between ED and YD were bigger than those due to the age discrimination. Second, on the contrary, the gaps of the hourly wages were caused by age discrimination more than characteristics between YD and ED. Third, it was not true that the elderly with disabilities take double discriminations in the labor market. It is therefore required to give the additional incentives to ED workers with government subsidies of employment promotion for the disabled provided. Second, it is proposed that the integrations the employment policies for the disabled with those for the aged because the disability as dependent valuable didn't have statistically significant effects on the employment probabilities and the hourly wage levels.

• Bulletin of the Korean Mathematical Society
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• v.50 no.5
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• pp.1651-1657
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• 2013

Let R be a prime ring, I a nonzero ideal of R, $d$ a derivation of R, $m({\geq}1)$, $n({\geq}1)$ two fixed integers and $a{\in}R$. (i) If $a((d(x)y+xd(y)+d(y)x+yd(x))^n-(xy+yx))^m=0$ for all $x,y{\in}I$, then either $a=0$ or R is commutative; (ii) If $char(R){\neq}2$ and $a((d(x)y+xd(y)+d(y)x+yd(x))^n-(xy+yx)){\in}Z(R)$ for all $x,y{\in}I$, then either $a=0$ or R is commutative.

• Journal of Plant Biology
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• v.26 no.2
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• pp.101-117
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• 1983

The phenological phenomena in terms of year day index (YDI) in South Korea were studied. The YDI was proposed here, because the remainer index such as Nuttonson's index is unadequate for the interpretation on the phenological phenomena of early spring season in sourthern coastal area. The YDI was calculated by summing daily mean temperature of the year days (YD) above physical zero degree in centigrade, based on the data of the Monthly Weather Reports from 1967 to 1980 by the Central Meteorological Office. The pattern of YDI increase with the increase of YD was similar to that of the remainder index such as the Nuttonson's index. The some YDI distribution maps were made by Yim and Kira (1975), dividing into 30'$\times$40' meshes, in latitude and longtude, on the topographical map(1 : 500,000) of the Korea Peninsula. According to the year day of different localities flowering dates of Prunus yedoensis and other phenological phenomena in various species delayed about 3.5 day as the increase of 1 degree of latitude, which coincides with the Hopkins bioclimatic law. It was found that the YDI is useful to interprete the phenology of plant and animal species and to select the optimum range of cultivars in South Korea.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2018.11a
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• pp.127-129
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• 2018

RadarBeacons are navigational equipment that helps the navigators avoid dangerous rocks, such as heavy fog, nighttime, etc. when ships operate, or when visibility is bad. The existing antenna was researching the development of the advanced radar eacon (Enhanced Radar Beacon) for improving the development of the next generation racon with the AIS (Automatic Identification System) function.

• Journal of the Korean Mathematical Society
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• v.46 no.5
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• pp.997-1005
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• 2009

Let R be a prime ring, I a nonzero ideal of R, d a derivation of R and n a fixed positive integer. (i) If (d(x)y+xd(y)+d(y)x+$yd(x))^n$ = xy + yx for all x, y $\in$ I, then R is commutative. (ii) If char R $\neq$ = 2 and (d(x)y + xd(y) + d(y)x + $yd(x))^n$ - (xy + yx) is central for all x, y $\in$ I, then R is commutative. We also examine the case where R is a semiprime ring.

• Journal of Microbiology and Biotechnology
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• v.25 no.9
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• pp.1401-1409
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• 2015

The aim of this study was to develop a SYBR Green-based real-time PCR assay for the rapid, specific, and sensitive detection of Acidovorax avenae subsp. citrulli, which causes bacterial fruit blotch (BFB), a serious disease of cucurbit plants. The molecular and serological methods currently available for the detection of this pathogen are insufficiently sensitive and specific. Thus, a novel SYBR Green-based real-time PCR assay targeting the YD-repeat protein gene of A. avenae subsp. citrulli was developed. The specificity of the primer set was evaluated using DNA purified from 6 isolates of A. avenae subsp. citrulli, 7 other Acidovorax species, and 22 of non-targeted strains, including pathogens and non-pathogens. The AC158F/R primer set amplified a single band of the expected size from genomic DNA obtained from the A. avenae subsp. citrulli strains but not from the genomic DNA of other Acidovorax species, including that of other bacterial genera. Using this assay, it was possible to detect at least one genomeequivalents of the cloned amplified target DNA using 5 × 10⁰ fg/µl of purified genomic DNA per reaction or using a calibrated cell suspension, with 6.5 colony-forming units per reaction being employed. In addition, this assay is a highly sensitive and reliable method for identifying and quantifying the target pathogen in infected samples that does not require DNA extraction. Therefore, we suggest that this approach is suitable for the rapid and efficient diagnosis of A. avenae subsp. citrulli contaminations of seed lots and plants.

• Korean Journal of Plant Resources
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• v.31 no.1
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• pp.16-23
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• 2018

Paeonia japonica is a perennial flowering plant used in traditional medicine therapy. The purpose of this study was to investigate the effect of water extract and solvent fractions obtained from P. japonica on anti-oxidative, anti-thrombin, anti-invasive and pro-apoptotic activities in YD-10B cells, human oral squamous carcinoma cell line. Water fraction revealed the highest extraction yield at 11.44% (w/w). Anti-oxidative activity was the highest in ethyl acetate fraction (85.13%). In the thrombin inhibitory activity test, ethyl fraction was the highest, with a value of 87.54%. Release and activation of MMP-2/pro-MMP-2 ratio in thrombin-treated YD-10B cells were significantly inhibited in the ethyl acetate fraction. At a concentration of $120{\mu}g/m{\ell}$ water extract and solvent fractions of P. japonica inhibited cell proliferation in YD-10B cells except water fraction. Pro-apoptotic effect on human oral squamous carcinoma cell using the Bax/Bcl-2 ratio analysis was higher in water extract than other fractions. These findings suggest that the ethyl acetate fraction of P. japonica potentiates a promising antioxidant, anti-thrombin and anti-invasive agents.

• International Journal of Oral Biology
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• v.32 no.2
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• pp.79-84
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• 2007

Oral squamous carcinoma (OSC) is the most common malignant neoplasm of the oral mucosa. Although the etiology of OSC is not fully understood, accumulated evidences indicate that the activation of proto-oncogenes and the inactivation of tumor suppressor genes underlie the disease development. An OSC cell line, YD-9 was newly established and characterized. However, the mutational analysis of p53 gene was not performed. Thus, in this study, the presence of mutation in the p53 gene was examined by amplification of exon-4 to -8 and subsequent DNA sequencing. Two point mutations were found in exon-4 and -6: A to G, resulting in amino acid change Tyr to Cys in exon-4, and C to G, resulting in amino acid change Gly to Arg in exon-6, respectively. Any mutation was not found in the exon-5, -7 and -8. The presented results would contribute to basic research to understand the biological mechanism of OSC using YD-9 cells.

• Journal of Korean society of Dental Hygiene
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• v.20 no.5
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• pp.763-771
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• 2020

Objectives: Erigeron bonariensis is a type of Erigeron found throughout the tropical and subtropical areas as one of the perennial plants or pioneer plants. It is known to show detoxifying, antipyretic, and anticancer effects for various cancers. However, there are no reports on the anticancer effect of E. bonariensis on oral cancer cells. In the present study, we investigated the effects of the methanol extract of Erigeron bonariensis (MEEB) on the inhibition of cell growth and induction of apoptosis in mucoepidermoid carcinoma (MEC) cell lines, including the MC3 and YD15 oral cancer cells. Methods: MC3 Cells were treated by dimethyl sulfoxide (DMSO) or methanol extracts of 20 various natural products 20 ㎍/mL for 48 hours and cell viability were analyzed as Trypan blue exclusion assay. The effects of MEEB treatment on the cell viability of MC3 and YD15 cells, for 48 h, were analyzed by Trypan blue exclusion assay. The anticancer efficacy and apoptosis of oral cancer cell lines were analyzed by western blot analysis. The statistical significance of differences between groups was analyzed by Student's two-tailed t-test. A value of P<0.05 compared to the vehicle control was considered statistically significant. Results: Among 20 different naturally derived products, MEEB significantly inhibited cell viability and increased cleaved poly [ADP-ribose] polymerase 1 (PARP) protein in the MC3 and YD15 cells in a concentration-dependent manner. Conclusions: These results suggest that MEEB can be used as a natural anticancer drug for the treatment of human oral cancer.