• Bulletin of the Korean Chemical Society
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• 제33권1호
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• pp.128-136
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• 2012

We report the solid-phase library construction of 222 number of a novel N-[alkyl sulfonamido-spiro(2H-1-benzopyran-2,4-piperidine)-6-yl] substituted amide 1A and amine 1B derivatives. The polymer-bound N-[alkylsulfonamido-spiro(2H-1-benzopyran-2,4-piperidine)-6-yl] substituted amide 9 and amine 10 derivatives were obtained by first diversity generation with various acid chlorides and alkyl halides. Further reactions on the resins 9 and 10 with substituted sulfonyl chlorides produced the desired N-[alkylsulfonamido-spiro(2H-1-benzopyran-2,4-piperidine)-6-yl] substituted amide 1A and amine 1B analogues.

• The Plant Pathology Journal
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• 제31권2호
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• pp.108-114
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• 2015

Curvularia lunata is an important maize foliar fungal pathogen that distributes widely in maize growing area in China, and several key pathogenic factors have been isolated. An yeast two-hybrid (Y2H) library is a very useful platform to further unravel novel pathogenic factors in C. lunata. To construct a high-quality full length-expression cDNA library from the C. lunata for application to pathogenesis-related protein-protein interaction screening, total RNA was extracted. The SMART (Switching Mechanism At 5' end of the RNA Transcript) technique was used for cDNA synthesis. Double-stranded cDNA was ligated into the pGADT7-Rec vector with Herring Testes Carrier DNA using homologous recombination method. The ligation mixture was transformed into competent yeast AH109 cells to construct the primary cDNA library. Eventually, a high qualitative library was successfully established according to an evaluation on quality. The transformation efficiency was about $6.39{\times}10^5$ transformants/$3{\mu}g$ pGADT7-Rec. The titer of the primary cDNA library was $2.5{\times}10^8cfu/mL$. The numbers for the cDNA library was $2.46{\times}10^5$. Randomly picked clones show that the recombination rate was 88.24%. Gel electrophoresis results indicated that the fragments ranged from 0.4 kb to 3.0 kb. Melanin synthesis protein Brn1 (1,3,8-hydroxynaphthalene reductase) was used as a "bait" to test the sufficiency of the Y2H library. As a result, a cDNA clone encoding VelB protein that was known to be involved in the regulation of diverse cellular processes, including control of secondary metabolism containing melanin and toxin production in many filamentous fungi was identified. Further study on the exact role of the VelB gene is underway.

• Bulletin of the Korean Chemical Society
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• 제35권8호
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• pp.2385-2390
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• 2014

Novel 2,6-difuctionalized 2H-benzopyrans were synthesized and evaluated for a sphingosylphosphorylcholine(SPC) inhibitor. The synthetic 2H-benzopyrans 1c and 3a showed high potency in SPC-induced cell proliferation assay ($IC_{50}$ < 20 nM). Neither hERG $K^+$ channel binding (> $10{\mu}M$) nor CYP inhibitions (> $10{\mu}M$) were observed. Also, the simple structure-activity relationship (SAR) results were obtained from analysis of 2H-benzopyran derivatives 1-3 and the anti-SPC effect of 2H-benzopyran 1c was confirmed by a HUVEC tube formation assay.

• 한국수정란이식학회지
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• 제13권2호
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• pp.159-172
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• 1998

사람 성장호르몬 수용체(hGHR) cDNA는 PCR방법에 의하여 fagment로서 보고되어진 바 있으나, liver cDNA로 부터 전장을 cloning한 보고는 없는 실정으로 본 연구에서는 기능을 가진 약 4.6kbp의 cDNA hGHR을 cloning 하는데 성공하였다. 먼저 cloning하기 위하여 human liver mRNA와 human breast cancer tissue로부터 회수한 mRNA를 RT-PCR방법에 의하여 human cDNA library와 cloning에 필요한 probe를 제작하였다. human library mRNA는 GT-PCR방법에 의하여 증폭하여 증폭되어진 산물은 λZAP Vector를 이용하여 cDNA library를 구축하였고,screeing을 위하여 임 보고 되어진 hGHR fragment native sequence를 기초로 N-terminal부분의 primer를 설계하여 950bp의 probe를 얻는데 성공하였다. 이 probe를 이용하여 준비된 human liver cDNA library로부터 2.5$\times$10 6개의 plaque로부터 6개의 positive clone을 획득하였고, 이들중 poly Asignal인 "AATAAA"를 포함하고 있는 가장 긴 약 3.8kbp의 clone을 sequencing한 결과 open reading frame을 포함하고 있었으나, 5'부분의 결손되어 있었다. 그리하여 이 부분은 human breast cancer tissue로 부터 회수한 mRNA를 RT-PCR에 의하여 증폭하였고, sequencing결과 이미 보고되어진 native hGHR와 비교한 결과 하나의 nucleotide가 silent mutation으로 판명되었다.한편 human liver cDNA library로부터 cloning한 3.8cp의 positive clone의 5'end의 결손된 부분에 silent mutation된 PCR 산물을 연결함으로써 native hGHR와 유사한 cDNA hGHR subcloning에 성공하였다. 이러한 cDNA hGHR의 clone이 function을 가지고 있는지를 검토하기 위하여 eukaryotic 발현 vector인 pCXN2에 의거 ligation한 후 chinese hamster ovary cell[CHO-KI]에 transfect를 실시하였다. Dexamethasone은 첨가하지 않고 hGH만의 존재하에서 이들 cell을 배양시키고 cell menbrane에서 발현 여부를 판정키 위하여 hGHR monocloual antibody를 사용하여 flow cytometery해석을 실시하는 한편 125I-hGH binding assay에 의하여 hGH binding activity를 측정하였다. 최종적으로 GH signal transduction의 target genedf으로 알려져 있는 serine protease inhibitor 2.1(Spi 2.1) gene의 promotor activity를 검토한 결과 hGHR을 transfect한 CHO Cell에 있어서 hGH의 농도에 의존적으로 증가되었다. 따라서 본 실험에서 cloning한 cDNA hGHR는 native hGHR와 같은 기능을 가지는 것으로 판명되었다.것으로 판명되었다.

• Bulletin of the Korean Chemical Society
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• 제32권10호
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• pp.3752-3755
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• 2011

• 한국작물학회:학술대회논문집
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• 한국작물학회 1999년도 춘계 학술대회지
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• pp.22-23
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• 1999

• 한국CDE학회논문집
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• 제11권4호
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• pp.273-288
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• 2006

Information integration of heterogeneous digital parts libraries or electronic pars catalogs is one of issues in B2B procurements. We need to provide an integrated view for multiple information sources. Utilization of ontologies as the metadata descriptions of the information sources can provide an integrated view. However, in order to integrate independently developed ontologies, the mismatches among them should be resolved. In this paper, we propose an ontology of meta-concepts, i.e. meta-ontology. The meta-concepts play the role of vocabulary to describe the parts library ontologies and provide well-established ontological semantics that helps the ontology modelers to consistently identify parts library concepts and systematically structure them. Consequently, the meta-ontology reduces the differences in the way the parts knowledge is interpreted and ensures the mismatches are confined to manageable mis matches, so that a software program can merge automatically. Modeling ontologies of mold and die parts libraries for B2B e-commerce is taken as an example to show how to use the meta-ontology. We also discuss how a parts library mediation system can automatically merge the well-structured parts library ontologies.

• 한국문헌정보학회지
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• 제42권2호
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• pp.371-392
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• 2008

본 논문에서는 H공공도서관의 도서관 온라인 열람목록(OPAC) 시스템에 구축되어 있는 장서와 대출기록 및 이용자 데이터를 수집하여 이를 분석하고 그 결과를 공공도서관 운영에 적용할 수 있는 방안을 제시하였다. 수집된 자료는 H도서관에서 소장하고 있는 대출이 가능한 단행본 총 57,927권의 서지데이타와 11,871명의 이용자 데이터, 대출기록 27,145건이었다. 대출기록 분석 데이터에서 관계변수로 장서유형, 대출빈도, 대출권수와 대출횟수, 출판년도, 이용요인을 추출하여 상관계수와 비모수검증법 등의 다양한 통계적 기법으로 분석하였다.

• 보존과학회지
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• 제28권4호
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• pp.387-394
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• 2012

전문적인 보존시설이 없는 대부분의 도서관에서는 손상된 도서의 간단한 수선 처리 및 응급조치 시 일반시판용 합성수지 접착제를 사용하고 있고, 이는 크게 PVAc 계열(Pa), 아크릴 계열(Ac)과 PVP 계열(Pv)로 나눠진다. 국립중앙도서관 자료보존실에서 사용하고 있는 보존용 합성수지 접착제(Pa-1)와 일반시판용 합성수지 접착제 중 대표적인 접착제를 골라 열화특성을 연구하기 위해 인공열화를 진행한 뒤, 약 한 달 동안 산성도(pH)와 색도를 측정하였다. 산성도 실험 결과에서 Pa-1(pH 7~7.5)과 Ac-2(pH 6.5~8)는 보존처리에 적합한 범위를 나타내었다. 색도실험에서 황변도를 나타내는 ${\Delta}b^*$는 Pa-1

• Journal of Information Science Theory and Practice
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• 제5권2호
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• pp.48-61
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• 2017

This paper uses 601 cited papers of Pakistani LIS researchers with the purpose to examine the individual performance of these Library and Information Science (LIS) researchers in terms of their research output and its impact on the LIS (national/international) literature by using various bibliometric indicators. A list of 139 authors was compiled with the help of the Library, Information Science, and Technology Abstracts (LISTA) and some other sources. Data were collected from Google Scholar and SPSS version 20 was utilized in order to identify the relationship between self-citations and various performance indices of the authors. The average citations received per paper vary from 1.80 to 10.08. About half of the papers were single-authored whereas less than one-fifth were by three or more authors. The authors who worked in collaboration produced more papers and received more citations. The h-index, g-index, hI-index, hI-norm, and e-index were used to determine the rank for each author. The intra-group citations grid revealed the volume of self-citations and a small group who cite each other more due to close academic and social relationships. The correlations between self-citations and the impact indices used revealed significant differences. Findings are useful for concerned institutions regarding award, promotions, etc. Further, future research should seriously consider the self-citations and social networking of authors while examining their citations-based research performance.