통합 검색 | Korea Science

박건용;박창혁;박병기
- 한국염색가공학회지
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- 제13권5호
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- pp.312-319
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- 2001
Kinetics and mechanism for alkaline hydrolysis of C. I. Disperse Blue 79(B-79) which is 4-N, N- diacetoxyethyl -2- acylamino-5-ethos y -2'-bromo-4',6'-dinitroazobenzene were investigated. The color strength of B-79 in acetone/water solutions of various NaOH concentrations decreased continuously. The hydrolysis rate of B-79 increased with increasing alkali concentration and appeared following first order reaction. The observed rate constants for various concentrations of B-79 showed similar values, and B-79 was hydrolyzed by first order reaction for dye concentration. Therefore, it was confirmed that the overall reaction follow second order kinetics and proceed via $S_N2$ reaction. From the study on kinetics and spectrometric analysis, it was proposed that the rate determining step of the hydrolysis reaction of B-79 is the nucleophilic substitution reaction - that is the reaction of the rapid attack of OH- on the carbon atom, which is in acceptor ring, adjacent to auto group to break the C-N bond. And it was also found that the final hydrolysis products of B-79 include both the acceptor ring in the form of sodium salt and the donor ring possessing 4-N,N-dihydroxyethyl group converted from 4-N, N-diacetoxyethyl group.
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최종열;장병호
- 한국작물학회지
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- 제26권1호
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- pp.51-55
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- 1981
한국에서 재배되고 있는 대두에서의 trypsin inhibitor variant의 분포를 조사하기 위하여 51 품종의 종실 단백질을 disc electrophoresis에 의하여 분리했다. 9품종이 Rf 0.79 band를, 그리고 42품종이 Rf 0.83 band를 갖고 있었으며, Rf 0.79의 분리비율은 17.6% 이었다. 금강대입(Rf 0.79 band)과 의두(Rf 0.83band)의 F_1은 Rf 0.79 band와 Rf 0.83 band를 모두 갖고 있었으며, F_2에서의 Rf 0.79:Rf 079/Rf 0.83:Rf 083의 분리는 22:53:21로서 이들 soybean trypsin inhibitor variant가 1쌍의 codominant alleles에 의하여 지배됨을 나타냈다.
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