• Title/Summary/Keyword: Xavier

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Evaluation of Antioxidant Potential and UV Protective Properties of Four Bacterial Pigments

  • Rupali Koshti;Ashish Jagtap;Domnic Noronha;Shivali Patkar;Jennifer Nazareth;Ruby Paulose;Avik Chakraborty;Pampi Chakraborty
    • Microbiology and Biotechnology Letters
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    • v.50 no.3
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    • pp.375-386
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    • 2022
  • In the present study, four distinctly colored bacterial isolates that show intense pigmentation upon brief ultraviolet (UV) light exposure are chosen. The strains are identified as Micrococcus luteus (Milky yellow), Cryseobacterium pallidum (Yellow), Cryseobacterium spp. (Golden yellow), and Kocuria turfanensis (Pink) based on their morphological and 16S rDNA analysis. Moderate salinity (1.25%), 25-37℃ temperature, and pH of 7.2 are found to be the most favorable conditions of growth and pigment production for all the selected isolates. The pigments are extracted using methanol: chloroform (1:1) and the purity of the pigments are confirmed by high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC). Further, Fourier transform infrared (FTIR) and UV-Visible spectroscopy indicate their resemblance with carotenoids and flexirubin family. The antioxidant activities of the pigments are estimated, and, all the pigments have shown significant antioxidant efficacy in 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picryl-hydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) assays. The UV protective property of the pigments is determined by cling-film assay, wherein, at least 25% of UV sensitive Escherichia coli survive with bio-pigments even after 90 seconds of UV exposure compared to control. The pigments also hold a good sun protective factor (SPF) value (1.5-4.9) which is calculated with the Mansur equation. Based on these results, it can be predicted that these bacterial pigments can be further developed into a promising antioxidant and UV-protectant for several biomedical applications.

A mathematical model of the commercial harvest of Palmaria palmata (Palmariales, Rhodophyta) on Digby Neck, Nova Scotia, Canada

  • Lukeman, Ryan J.;Beveridge, Leah F.;Flynn, Andrea D.;Garbary, David J.
    • ALGAE
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    • v.27 no.1
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    • pp.43-54
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    • 2012
  • A mathematical model of the commercial harvest of Palmaria palmata (Dulse) is presented based on a logistic model and field data collected on Digby Neck, Nova Scotia from 14 harvested shores during May to August, 2010. Field observations used to estimate model parameters included cover of Dulse before and after harvest from Dulse dominated boulders for which surface area was estimated, and from which fresh biomass of harvested Dulse was weighed. Over all the surveys the average harvest fraction was about 50%, and the total resource was about $1,600g\;m^{-2}$. With growth rates in excess of 4% per day and a 50% harvest of the standing crop each month, the model suggests that the Dulse resource is sustainable at current harvest levels.

Cell Division in the Absence of Mitosis: The Unusual Case of the Fucoid Ascophyllum nodosum (L.) Le Jolis (Phaeophyceae)

  • Garbary, David J.;Lawson, Greg;Clement, Kelly;Galway, Moira E.
    • ALGAE
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    • v.24 no.4
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    • pp.239-248
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    • 2009
  • Fluorescence and electron microscopy were used to examine epidermal shedding in the fucoid alga, Ascophyllum nodosum. Mature meristoderm cells are ca. 50-100 x 30-40 ${\mu}m$ and highly polarized, with a single nucleus and chloroplasts near the base of the cell. Nuclei in these cells undergo mitosis when they are dividing to form a new cortical cell towards the middle of the frond, or anticlinal divisions as part of frond elongation. However, cytokinesis also occurs regularly in these cells when a new periclinal wall is deposited at about 30% of the cell length from the apical end. The newly formed distal cells are anucleate and without chloroplasts. Following cytokinesis the tangential walls then break at the thinnest point. The whole process is synchronous in adjoining epidermal cells across large areas of the frond surface, and this layer dehisces from the thallus. This is the only known plant or algal system in which cytokinesis regularly occurs in the absence of mitosis. We consider this process a novel form of programmed cell death.

MONOPHONIC PEBBLING NUMBER OF SOME NETWORK-RELATED GRAPHS

  • AROCKIAM LOURDUSAMY;IRUDAYARAJ DHIVVIYANANDAM;SOOSAIMANICKAM KITHER IAMMAL
    • Journal of applied mathematics & informatics
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    • v.42 no.1
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    • pp.77-83
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    • 2024
  • Chung defined a pebbling move on a graph G as the removal of two pebbles from one vertex and the addition of one pebble to an adjacent vertex. The monophonic pebbling number guarantees that a pebble can be shifted in the chordless and the longest path possible if there are any hurdles in the process of the supply chain. For a connected graph G a monophonic path between any two vertices x and y contains no chords. The monophonic pebbling number, µ(G), is the least positive integer n such that for any distribution of µ(G) pebbles it is possible to move on G allowing one pebble to be carried to any specified but arbitrary vertex using monophonic a path by a sequence of pebbling operations. The aim of this study is to find out the monophonic pebbling numbers of the sun graphs, (Cn × P2) + K1 graph, the spherical graph, the anti-prism graphs, and an n-crossed prism graph.

Population ecology of Palmaria palmata (Palmariales, Rhodophyta) from harvested and non-harvested shores on Digby Neck, Nova Scotia, Canada

  • Garbary, David J.;Beveridge, Leah F.;Flynn, Andrea D.;White, Katelyn L.
    • ALGAE
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    • v.27 no.1
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    • pp.33-42
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    • 2012
  • Population ecology of Palmaria palmata is described from the intertidal zone of Digby Neck and adjacent islands of Nova Scotia. The primary objectives were: to evaluate the difference in habitat specialization and population structure of P. palmata between harvest and non-harvest shores, and to characterize differences in thallus structure and frond sizes between epilithic and epiphytic populations. Harvest shores were gently sloping boulder fields with boulders typically about 0.5-1.0 m with dense cover of P. palmata on many of the rocks. Non-harvest shores (with or without P. palmata) consisted of boulders that were smaller or larger than harvest shores, or bedrock; when P. palmata was present on nonharvest sites it was typically epiphytic on other algae (e.g., Fucus spp., Mastocarpus stellatus, Devaleraea ramentacea). Harvestable epiphytic populations occurred only in high current areas. While there was little difference in average cover of P. palmata harvest and non-harvest shores ($31.2{\pm}13.7%$ vs. $19.4{\pm}7.3%$, mean ${\pm}$ standard deviation [SD]), the cover of P. palmata on harvest shores was highly skewed such that individual boulders often had >90% cover while adjacent rocks had little. Frond length of large fronds was greater on harvested shores, and mean frond density ($g\;m^{-2}$) was three times higher than the mean density on the non-harvested shores. Frond lengths of entire epiphytic and epilithic frond complements of 119 thalli from harvest beaches showed no difference in mean size of the largest fronds, and no difference in frond number per holdfast when epiphytic and epilithic thalli were compared.

Temporal and spatial variation in the distribution of life history phases of Chondrus crispus (Gigartinales, Rhodophyta)

  • Garbary, David J.;Tompkins, Elizabeth;White, Katelyn;Corey, Peter;Kim, Jang-K.
    • ALGAE
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    • v.26 no.1
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    • pp.61-71
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    • 2011
  • Thirty populations of Chondrus crispus Stackhouse from Nova Scotia were collected during the years 1993 to 2011. Taken from estuaries, wave exposed open coasts, high intertidal rock pools and shallow subtidal habitats, the populations were evaluated for relative abundance of tetrasporophytic and gametophytic life history phases. Over 2,800 thalli were characterized using the resorcinol-acetal test to distinguish the kappa- and lambda-carrageenan containing fronds of gametophytes and tetrasporophytes, respectively. These populations had $77{\pm}5%$ gametophytes (mean ${\pm}95%$ confidence interval), with most populations having gametophyte : sporophyte ratios ranging from 2 : 1 to 9 : 1. No population had a dominance of tetrasporophytes, although two populations had 1 : 1 ratios. A meta-analysis of our data along with previously published accounts showed no significant changes in gametophyte dominance with respect to hypothesized gradients of wave exposure, salinity, or water depth. Significant changes occurred in ratios at five sites where replicate sampling occurred in different years. We conclude that C. crispus in Maritime Canada has a natural ratio of 3 : 1 or greater in stable conditions, and that lower ratios represent recovery from disturbance in which bare substratum is created that is subsequently colonized by carpospores from remaining gametophytic thalli.

Post Modernism in Xavier Dolan's Movies -With a Focus on "I Killed My Mother(2009)" and "Mommy(2014)"- (자비에돌란 영화의 포스트 모더니즘 <아이킬드마이마더, 마미를 중심으로>)

  • Kim, Loyou
    • The Journal of the Korea Contents Association
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    • v.16 no.3
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    • pp.162-170
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    • 2016
  • Xavier Dolan delves into maternal instinct and sexual identity and the attempt and experiment of his movies are not difficult to understand and not unfamiliar. He communicates with audience through pastiche and self-reflexivity, rather than disassembling and destruction of style and form of existing films. Pastiche is the intertextuality between familiar genres such as film, poem, fashion, music, painting, etc. His films shows the characteristics of postmodernism, which include genre over, de-subjectivity, interest in public culture, and representation of nostalgia. This study recognizes anew the present of postmodernism gradually going out of fashion and analyzes the characteristics of postmodernism and repeated motif in his movies. This study also analyzes Xavier Dolan's interest in social minority and allegorian characteristics through characters appearing in his movies.

PEBBLING ON THE MIDDLE GRAPH OF A COMPLETE BINARY TREE

  • LOURDUSAMY, A.;NELLAINAYAKI, S. SARATHA;STEFFI, J. JENIFER
    • Journal of applied mathematics & informatics
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    • v.37 no.3_4
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    • pp.163-176
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    • 2019
  • Given a distribution of pebbles on the vertices of a connected graph G, a pebbling move is defined as the removal of two pebbles from some vertex and the placement of one of those pebbles at an adjacent vertex. The t-pebbling number, $f_t(G)$, of a connected graph G, is the smallest positive integer such that from every placement of $f_t(G)$ pebbles, t pebbles can be moved to any specified vertex by a sequence of pebbling moves. A graph G has the 2t-pebbling property if for any distribution with more than $2f_t(G)$ - q pebbles, where q is the number of vertices with at least one pebble, it is possible, using the sequence of pebbling moves, to put 2t pebbles on any vertex. In this paper, we determine the t-pebbling number for the middle graph of a complete binary tree $M(B_h)$ and we show that the middle graph of a complete binary tree $M(B_h)$ satisfies the 2t-pebbling property.