• Title/Summary/Keyword: Xanthomonas oryzae pv.

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Studies on Manifestation of Bacterial Leaf Blight Resistant Gene I. Relationship Between the Resistance of Rice to Bacterial Leaf Blight and the Multiplication and Spread of the Xanthomonas campestris pv. oryzae (수도 흰잎마름병 저항성 유전자 발현에 관한 연구 I. 흰잎마름병균의 증식 및 이동과 저항성과의 관계)

  • 김한용;최재을
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.35 no.2
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    • pp.132-136
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    • 1990

  • This experiment was conducted to study the multiplication and spread of bacterial population in water exuded through the hydathode of infected leaf of Xanthomonas campestris pv. oryzae in resistant and susceptible rice cultivars to bacterial leaf blight. The results obtained are summarized as follows. The bacterial multiplication in resistant cultivars was almost constant from three days to twelve days after inoculation with population of 10$^3$-10$^4$cfu/$\textrm{cm}^2$, but the multiplication was increased as days after inoculation extended in susceptible cultivars. The speed of bacterial multiplication and the number of bacteria spread above and below the inoculated position were closely related with the resistance of rice cultivars to bacterial leaf blight. The bacterial multiplication and spread were observed throughout the all growing stages including seedling, maximum tillering and flag leaf stages. The bacterial populations in water exuded through the hydathode were dependent on the multiplication and spread, and the populations were also closely related with the resistance of rice cultivars.

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A Rapid and Simple Detection Assay for Rice Bacterial Leaf Blight by Recombinase Polymerase Amplification (벼 흰잎마름병의 신속하고 간편한 진단을 위한 Recombinase Polymerase Amplification 등온증폭법)

  • Kim, Shinhwa;Lee, Bong Choon;Kim, Hyun Ju;Choi, Soo Yeon;Seo, Su Jwa;Kim, Sang-Min
    • Research in Plant Disease
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    • v.26 no.4
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    • pp.195-201
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    • 2020

  • Rice bacterial leaf blight (BLB) by Xanthomonas oryzae pv. oryzae (Xoo) is considered to be one of the major rice diseases steadily occurring around the rice-producing countries. In this study, we developed a recombinase polymerase amplification (RPA) assay for the rapid, convenient and specific diagnosis of Xoo by targeting Xoo-specific transposase A gene. As the target gene can be amplified in 10 min without DNA extraction process and special equipment for temperature control, RPA for BLB can be useful and practical component for on-site diagnosis.

  • https://doi.org/10.5423/RPD.2020.26.4.195 인용 PDF KSCI