• Title/Summary/Keyword: XaiF

Search Result 5, Processing Time 0.018 seconds

Characterization of the xaiF Gene Encoding a Novel Xylanase-activity- increasing Factor, XaiF

  • Cho, Ssang-Goo;Choi, Yong-Jin
    • Journal of Microbiology and Biotechnology
    • /
    • v.8 no.4
    • /
    • pp.378-387
    • /
    • 1998
  • The DNA sequence immediately following the xynA gene of Bacillus stearothermophilus 236 [about l-kb region downstream from the translational termination codon (TAA) of the xynA gene]was found to have an ability to enhance the xylanase activity of the upstream xynA gene. An 849-bp ORF was identified in the downstream region, and the ORF was confirmed to encode a novel protein of 283 amino acids designated as XaiF (xylanase-activity-increasing factor). From the nucleotide sequence of the xaiF gene, the molecular mass and pI of XaiF were deduced to be 32,006 Da and 4.46, respectively. XaiF was overproduced in the E. coli cells from the cloned xaiF gene by using the T7 expression system. The transcriptional initiation site was determined by primer extension analysis and the putative promoter and ribosome binding regions were also identified. Blast search showed that the xaiF and its protein product had no homology with any gene nor any protein reported so far. Also, in B. subtilis, the xaiF trans-activated the xylanase activity at the same rate as in E. coli. In contrast, xaiF had no activating effect on the co-expressed ${\beta}-xylosidase$ of the xylA gene derived from the same strain of B. stearothermophilus. In addition, the intracellular and extracellular fractions from the E. coli cells carrying the plasmid-borne xaiF gene did not increase the isolated xylanase activity, indicating that the protein-protein interaction between XynA and XaiF was not a causative event for the xylanase activating effect of the xaiF gene.

  • PDF

A Proposal of Sensor-based Time Series Classification Model using Explainable Convolutional Neural Network

  • Jang, Youngjun;Kim, Jiho;Lee, Hongchul
    • Journal of the Korea Society of Computer and Information
    • /
    • v.27 no.5
    • /
    • pp.55-67
    • /
    • 2022
  • Sensor data can provide fault diagnosis for equipment. However, the cause analysis for fault results of equipment is not often provided. In this study, we propose an explainable convolutional neural network framework for the sensor-based time series classification model. We used sensor-based time series dataset, acquired from vehicles equipped with sensors, and the Wafer dataset, acquired from manufacturing process. Moreover, we used Cycle Signal dataset, acquired from real world mechanical equipment, and for Data augmentation methods, scaling and jittering were used to train our deep learning models. In addition, our proposed classification models are convolutional neural network based models, FCN, 1D-CNN, and ResNet, to compare evaluations for each model. Our experimental results show that the ResNet provides promising results in the context of time series classification with accuracy and F1 Score reaching 95%, improved by 3% compared to the previous study. Furthermore, we propose XAI methods, Class Activation Map and Layer Visualization, to interpret the experiment result. XAI methods can visualize the time series interval that shows important factors for sensor data classification.

Anomaly Detection using VGGNet for safety inspection of OPGW (광섬유 복합가공 지선(OPGW) 설비 안전점검을 위한 VGGNet 기반의 이상 탐지)

  • Kang, Gun-Ha;Sohn, Jung-Mo;Son, Do-Hyun;Han, Jeong-Ho
    • Proceedings of the Korean Society of Computer Information Conference
    • /
    • 2022.01a
    • /
    • pp.3-5
    • /
    • 2022
  • 본 연구는 VGGNet을 사용하여 광섬유 복합가공 지선 설비의 양/불량 판별을 수행한다. 광섬유 복합가공 지선이란, 전력선의 보호 및 전력 시설 간 통신을 담당하는 중요 설비로 고장 발생 전, 결함의 조기 발견 및 유지 관리가 중요하다. 현재 한국전력공사에서는 드론에서 촬영된 영상을 점검원이 이상 여부를 점검하는 방식이 주로 사용되고 있으나 이는 점검원의 숙련도, 경험에 따른 정확성 및 비용과 시간 측면에서 한계를 지니고 있다. 본 연구는 드론에서 촬영된 영상으로 VGGNet 기반의 양/불량 판정을 수행했다. 그 결과, 정확도 약 95.15%, 정밀도 약 96%, 재현율 약 95%, f1 score 약 95%의 성능을 확인하였다. 결과 확인 방법으로는 설명 가능한 인공지능(XAI) 알고리즘 중 하나인 Grad-CAM을 적용하였다. 이러한 광섬유 복합가공 지선 설비의 양/불량 판별은 점검원의 단순 작업에 대한 비용 및 점검 시간을 줄이며, 부가가치가 높은 업무에 집중할 수 있게 해준다. 또한, 고장 결함 발견에 있어서 객관적인 점검을 수행하기 때문에 일정한 점검 품질을 유지한다는 점에서 적용 가치가 있다.

  • PDF

Trustworthy AI Framework for Malware Response (악성코드 대응을 위한 신뢰할 수 있는 AI 프레임워크)

  • Shin, Kyounga;Lee, Yunho;Bae, ByeongJu;Lee, Soohang;Hong, Heeju;Choi, Youngjin;Lee, Sangjin
    • Journal of the Korea Institute of Information Security & Cryptology
    • /
    • v.32 no.5
    • /
    • pp.1019-1034
    • /
    • 2022
  • Malware attacks become more prevalent in the hyper-connected society of the 4th industrial revolution. To respond to such malware, automation of malware detection using artificial intelligence technology is attracting attention as a new alternative. However, using artificial intelligence without collateral for its reliability poses greater risks and side effects. The EU and the United States are seeking ways to secure the reliability of artificial intelligence, and the government announced a reliable strategy for realizing artificial intelligence in 2021. The government's AI reliability has five attributes: Safety, Explainability, Transparency, Robustness and Fairness. We develop four elements of safety, explainable, transparent, and fairness, excluding robustness in the malware detection model. In particular, we demonstrated stable generalization performance, which is model accuracy, through the verification of external agencies, and developed focusing on explainability including transparency. The artificial intelligence model, of which learning is determined by changing data, requires life cycle management. As a result, demand for the MLops framework is increasing, which integrates data, model development, and service operations. EXE-executable malware and documented malware response services become data collector as well as service operation at the same time, and connect with data pipelines which obtain information for labeling and purification through external APIs. We have facilitated other security service associations or infrastructure scaling using cloud SaaS and standard APIs.