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Production of the yellow fluorescent silk using the fibroin heavy chain protein expression system in transgenic silkworm (피브로인 H-chain 재조합 단백질 발현시스템을 이용한 황색형광실크의 제작)

  • Kim, Seong Wan;Choi, Kwang-Ho;Kim, Seong Ryul;Yun, Eun Young;Park, Seung Won;Kang, Seok Woo;Goo, Tae Won
    • Journal of Sericultural and Entomological Science
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    • v.52 no.2
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    • pp.102-109
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    • 2014
  • We constructed the fibroin H-chain expression system to produce enhanced yellow fluorescent proteins (EYFP) in the silk of transgenic silkworm. Fluorescent silk could be made by fusing EYFP cDNA to the heavy chain gene and injecting it into a silkworm. The EYFP fusion protein, each with N- and C-terminal sequences of the fibroin H-chain, was designed to be secreted into the lumen of the posterior silk glands. The expression of the EYFP/H-chain fusion gene was regulated by the fibroin H-chain promoter. The yellow fluorescence proving that the fusion protein was present in the silk. Accordingly, we suggest that the EYFP fluorescence silk will enable the production of novel biomaterial based on the transgenic silk.

Development of Cut-in Lane Changing Model Based on Observed Driver's Behavior in Uninterrupted Traffic Flow (연속교통류에서의 끼어들기 행태 분석 및 모형 개발)

  • Kim, Kyung-Hee;Chang, Myung-Soon;Kim, Jin-Tae;Kim, Eung-Cheol
    • Journal of Korean Society of Transportation
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    • v.22 no.6
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    • pp.145-157
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    • 2004
  • Microscopic freeway simulation models project the uncertain values of performance measures for subject traffic conditions by explaining drivers' driving behavior with lane changing and car-following models. However, the existing lane changing models are limited to gap acceptance oriented passive behavior of drivers and not able to capture more-or-less aggressive driving behavior(e.g. cut-in lane changing) ordinarily obseved in field. This paper suggests the definition of cut-inlane changing and presents its characteristics based on the findings from two different freeway on- and off-ramp sections. In addition, this paper proposes a new lane changing model capable of handling both passive and active drivers' driving behavior for better performance of simulations. The proposed lane changing model was tested with Hanyang Simulatin (HYTSIM), a microscopic freeway simulation program developed for this study. The HYTSIM simulation results reflecting the performance of the proposed lane changing model were compared against the field data. The test results showed that the distribution of gaps collected when vehicles change lanes were statistically identical to the field data at 95% confidence level.

사파이어 기판위에 성장된 GaN의 Bow 특성 연구

  • Seo, Yong-Gon;Sin, Seon-Hye;Kim, Du-Su;Yun, Hyeong-Do;Hwang, Seong-Min
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.08a
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    • pp.222-222
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    • 2013
  • GaN 기반 반도체는 넓은 bandgap을 가지고 있어 가시광부터 자외선까지 다양한 광전소자에 유용하게 사용된다. 광전소자중 발광다이오드의 경우 대부분 사파이어 기판위에 성장된다. 하지만 사파이어와 GaN의 격자 불일치 및 열팽창 계수의 차이로 인해 고품질의 GaN를 성장하기가 어렵다. 특히 열팽창 계수의 차이는 GaN 성장 공정이 고온에서 이루어지기 때문에 성장후 상온으로 온도가 떨어질 때 웨이퍼의 bowing을 발생시키고 동시에 dislocation이나 crack과 같은 결함이 생성되 GaN 성장막의 품질을 떨어트린다. 웨이퍼의 크기가 커지면 커질수록 웨이퍼 bowing은 커져 이에 대한 연구는 중요하다. 본 논문에서 2인치 사파이어 기판위에 성장된 GaN의 bow특성을 알아보기 위해 먼저 simulation을 하였고 실제로 성장된 GaN 웨이퍼와 비교를 하였다. c-plane 사파이어 기판위에 성장된 c-plane GaN의 bow특성을 알아보기 위해 성장 온도 $1,100^{\circ}C$에서 GaN두께를 1 ${\mu}m$에서 10 ${\mu}m$까지 1 ${\mu}m$씩 변화시켜 가며 simulation을 하였다. GaN두께가 1 ${\mu}m$일때는 bow가 11 ${\mu}m$, 6 ${\mu}m$ 일때는 54.7 ${\mu}m$, 10 ${\mu}m$ 일때는 108 ${\mu}m$를 얻어 GaN두께가 1 ${\mu}m$씩 증가할 때 마다 bow가 약 10 ${\mu}m$씩 증가하였다. 성장온도에 대한 영향을 알아보기 위해 $700^{\circ}C$에서 $1,200^{\circ}C$까지 $100^{\circ}C$씩 증가시켜며 bow특성 simulation을 하였다. 6 ${\mu}m$성장된 GaN의 경우 성장온도가 $100^{\circ}C$ 씩 증가할 때 bow는 약 6 ${\mu}m$ 증가하였다. 실제 성장된 c-plane GaN웨이퍼와 비교하기 위해 GaN을 각각 3 ${\mu}m$와 6 ${\mu}m$를 성장시켰고 high resolution x-ray diffraction장비를 사용하여 bow를 측정한 결과 각각 28 ${\mu}m$와 61 ${\mu}m$ 였고 simulation결과는 각각 33 ${\mu}m$와 65.5 ${\mu}m$를 얻어 비슷한 결과를 보였다. c-plane 사파이어 기판위에 성장된 c-plane GaN는 방향에 무관하게 동일한 bow 특성을 가지는 반해 r-plane 사파이어 기판위에 성장된 a-plane GaN는 방향에 따라 다른 bow특성을 보인다. a-plane GaN 이방향성적인 bow 특성을 알아보기 위해 simulation을 하였다. $1,100^{\circ}C$에서 a-plane GaN을 성장할 때 두께가 1 ${\mu}m$ 증가할 때마다 bow가 c축 방향으로는 21.7 ${\mu}m$씩 증가하였고 m축 방향으로는 11.8 ${\mu}m$ 씩 증가하여 매우 큰 이방향성적인 bow 특성을 보였다. 실제 r-plane 사파이어 기판위에 성장된 a-plane GaN의 bow를 측정하였고 simulation 결과와 비교해 보았다.

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Direct Imaging of Polarization-induced Charge Distribution and Domain Switching using TEM

  • O, Sang-Ho
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.08a
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    • pp.99-99
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    • 2013
  • In this talk, I will present two research works in progress, which are: i) mapping of piezoelectric polarization and associated charge density distribution in the heteroepitaxial InGaN/GaN multi-quantum well (MQW) structure of a light emitting diode (LED) by using inline electron holography and ii) in-situ observation of the polarization switching process of an ferroelectric Pb(Zr1-x,Tix)O3 (PZT) thin film capacitor under an applied electric field in transmission electron microscope (TEM). In the first part, I will show that strain as well as total charge density distributions can be mapped quantitatively across all the functional layers constituting a LED, including n-type GaN, InGaN/GaN MQWs, and p-type GaN with sub-nm spatial resolution (~0.8 nm) by using inline electron holography. The experimentally obtained strain maps were verified by comparison with finite element method simulations and confirmed that not only InGaN QWs (2.5 nm in thickness) but also GaN QBs (10 nm in thickness) in the MQW structure are strained complementary to accommodate the lattice misfit strain. Because of this complementary strain of GaN QBs, the strain gradient and also (piezoelectric) polarization gradient across the MQW changes more steeply than expected, resulting in more polarization charge density at the MQW interfaces than the typically expected value from the spontaneous polarization mismatch alone. By quantitative and comparative analysis of the total charge density map with the polarization charge map, we can clarify what extent of the polarization charges are compensated by the electrons supplied from the n-doped GaN QBs. Comparison with the simulated energy band diagrams with various screening parameters show that only 60% of the net polarization charges are compensated by the electrons from the GaN QBs, which results in the internal field of ~2.0 MV cm-1 across each pair of GaN/InGaN of the MQW structure. In the second part of my talk, I will present in-situ observations of the polarization switching process of a planar Ni/PZT/SrRuO3 capacitor using TEM. We observed the preferential, but asymmetric, nucleation and forward growth of switched c-domains at the PZT/electrode interfaces arising from the built-in electric field beneath each interface. The subsequent sideways growth was inhibited by the depolarization field due to the imperfect charge compensation at the counter electrode and preexisting a-domain walls, leading to asymmetric switching. It was found that the preexisting a-domains split into fine a- and c-domains constituting a $90^{\circ}$ stripe domain pattern during the $180^{\circ}$ polarization switching process, revealing that these domains also actively participated in the out-of-plane polarization switching. The real-time observations uncovered the origin of the switching asymmetry and further clarified the importance of charged domain walls and the interfaces with electrodes in the ferroelectric switching processes.

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The Changes of CO Gas Sensing Properties of ZnO and $SnO_2$ with Addition $TiO_2$ ($TiO_2$첨가에 의한 ZnO와 $SnO_2$의 일산화탄소 감응특성 변화)

  • Kim, Tae-Won;Choi, U-Sung;Jun, Seon-Taek
    • Korean Journal of Materials Research
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    • v.8 no.4
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    • pp.312-316
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    • 1998
  • ZnO- TiO$_2$, and Sn0$_2$ - Ti0$_2$ ceramic composites doped with TiO$_2$ were prepared and their electrical and 1000ppm CO gas sensing properties were investigated. The phases of samples were analyzed by XRD, and the microsturctures of the fractured surface of samples were observed by SEM. A carbon monoxide gas sensitivity was de¬fined as the ratio of the resistance in dry air atmosphere(R$drt air$) to the resistance in 1000ppm CO gas atmosphere(R$_co$) The CO gas sensitivity of Smol% Ti0$_2$-added ZnO decreased about 1.7 times compared to that of pure ZnO. On the other hand, the maximum CO gas sensitivity of Ti0$_2$-added SnO$_2$ increased about 2.5 times compared to that of pure SnO$_2$. Therefore, the CO gas sensitivies of SnO$_2$-TiO$_2$ composite were better than those of ZnO- Ti0$_2$ and the temper¬ature range showing the maximum sensitivity for Sn0$_2$-TiO$_2$ composite was lower than that for ZnO- Ti0$_2$.

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Human Health Factors and Traffic Accidents among Taxi Drivers in the Seoul Area (서울지역에 있어서 직업운전자의 건강상태가 교통사고에 미치는 영향)

  • Kim, Ihm-Soon;Lee, Kyung-Jong;Roh, Jae-Hoon;Moon, Young-Hahn
    • Journal of Preventive Medicine and Public Health
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    • v.22 no.3 s.27
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    • pp.313-322
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    • 1989
  • The present status of the traffic accident rate in Korea shows that it is the highest in the world with a continuously increasing trend. Human factors account for 90% of the causes of traffic accidents. Therefore, the purpose of this study was to determine some human factors related to traffic accidents by studying the relationship between health status and traffic accidents. To accomplish this purpose, all taxi companies located in the Seoul area were divided in three groups according to the number of taxi possessed, then some companies in each ?roup were randomly selected for study, and a total of 222 drivers in those selected companies were questioned and examined from April 15 to April 22, 1989. Seventy drivers among 222 had experienced a traffic accident. A $x^2$-test was performed on the data, then, factor analysis and discrminant analysis were executed with the following results: 1. The drivers complaining of gastroenteric symptoms numbered 110(49.5%), which was the major symptom among all drivers complaining of poor health. 2. In the primary analysis, variables related to traffic accidents were divided into general, occupational, and health characteristics. Drivers having no traffic accident experience and drivers having that experience were subjected to question about age, educational level, residential status, monthly average income, working hours and days, degree of satisfaction with their profession and homelife, degree of worry about health. degree of fatigue, medication, drunken driving, and illness, but there were no statistical significances. 3. In the factor analysis, the 8 health variables which cause traffic accidents were classified into 3 common factors which were perceived health factor, sleeping and drunken driving, and visual acuity and smoking factor. Perceived health was the factor which contributed most to explaining accidents. 4. In the discriminant analysis, a correct prediction rate of 68.0% was obtained in the factors of all the characteristics. 5. Degree of sttisfaction with their homelife and educational and economic factor in the general characteristics, degree of satisfaction with their profession in the occupational characteristics, and sleeping and drunken driving in the health characteristics were selected as statistically significant factors to discriminant the traffic accident. 6. Among the factors of the general, occupational, and health characteristics, degree of satisfaction with their homelife, driving experience, family factor, perceived factor were selected as the statistically significant factors.

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A Study on the Physical and Mental Health Factors affecting Industrial Accidents (산업재해 발생에 영향을 미치는 건강요인에 관한 연구)

  • Lee, Myung-Sun;Roh, Jae-Hoon;Moon, Young-Hahn
    • Journal of Preventive Medicine and Public Health
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    • v.22 no.3 s.27
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    • pp.355-367
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    • 1989
  • This study examined the physical and mental health factors affecting the industrial accidents of 142 injured and 1,212 uninjured workers in the shipbuilding industry from 1986 to 1988. The results acquired from the Todai Health Index (THI) and from analysis of the health examination were as follows: 1. Among the personal characteristics of the workers, the educational level of injured workers was significantly lower than that of the uninjured workers. 2. Among the physical characteristics, vision and $R\ddot{o}hrer$ Index of the injured workers were lower than those of the uninjured workers, and the difference was statistically significant. On the other hand, the differences in height, weight, hearing function, hematocrit, blood pressure, urine test, and X-ray findings were not statistically significant between the injured and uninjured workers. 3. The score of the THI questionnaire on the physical and mental health of the injured workers was higher than that of the uninjured workers, and the difference was statistically significant. 4. Form the THI score, the industrial workers had complained more about mental health than physical health and there was a statistically singinficant relation with the industrial accidents. 5. The relative risk expressed in terms of the odds ratio was 2.9 for poorer vision, 2.7 for a lower educational level, 2.2 for a higher THI score and 1.6 for overdrinking. 6 Educational level, vision, and the THI score were selected as significant factors influencing industrial accidents based on a log-linear model. According to the results of this model by logistic analysis, the odds ratio of industrial accidents was 1.8 for a lower educational level, 1.7 for poorer vision, and 1.6 for a higher THI score. 7 By event history analysis with the dependent variable as the duration of work at the time of the industrial accident, educational level, age, $R\ddot{o}hrer$ Index and THI score were the statistically significant variables selected, and the hazard rate of industrial accident occurrence was 0.24 for a lower educational level, 0.92 for age, 0.99 for a lower $R\ddot{o}hrer$ Index and 2.72 for a higher THI score. As we have seen, educational level and THI score were the most significant factors affecting the hazard rate of industrial accidents. Vision, $R\ddot{o}hrer$ Index, age, and drinking behavior were also statistically significant variables influencing industrial accidents. Therefore, in order to prevent industrial accidents, it is necessary to establish a health management plan for industry which can objectively evaluate not only the physical but also the mental health of the workers. If we use this type of study as a prospective study design, we can determine the relative risk of physical and mental health factors on industrial accidents. Furthermore, it is expected that this type of study will provide workers at high risk with more precise basic data for a health managment plan for industrial accident prevention.

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Effects of Sweet Bee Venom Pharmacopuncture Combined with Korean Medicine Treatment for Acute Low Back Pain Syndrome Patient: A Case Report (급성 요통 증후군 환자에 대해 봉독약침요법을 병행한 한의학적 치료 효과: 증례보고)

  • Bong, Sung Min;Jang, Woo Seok;Kim, Kyung Ho
    • Korean Journal of Acupuncture
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    • v.37 no.1
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    • pp.54-62
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    • 2020
  • Objectives : Acute low back pain syndrome causes pain and poor quality of life. There are various studies of SBV (Sweet bee venom) pharmacopuncture, but few have identified the therapeutic effects for patients unable to walk due to acute back pain. This case series report three cases of acute low back pain syndrome treated with SBV pharmacopuncture combined with Korean Medicine (KM) treatments. Methods : Three acute low back pain syndrome patients with no other acute abnormalities in the imaging were treated by KM treatments including SBV pharmacopuncture, acupuncture, cupping, herbal medicine and physical therapy. The improvement of symptoms was evaluated using Numerical rating scale (NRS), Oswestry disability Index (ODI), EuroQol-5 dimension index (EQ-5D) and EuroQol-visual analogue scale (EQ-VAS). Results : Patients who were unable to walk due to acute back pain were able to walk. There were significant improvements of NRS, ODI, EQ-5D and EQ-VAS after treatment. The patients experienced an average 90% decrease in low back pain after inpatient KM treatment for about 8 days. NRS decreased by an average of 78.9%, ODI decreased by an average of 49.4%, and EQ-VAS increased by an average of 92.6%. In the case 1, EQ-5D decreased in all categories, but in the case 2, it decreased in all categories except for pain/discomfort and anxiety/depression, but in the case 3, it decreased in all categories except anxiety/depression. There was no serious side effect after treatment of SBV pharmacopuncture. Conclusions : KM treatments including SBV pharmacopuncture may be effective for acute low back pain syndrome patients. However, randomized controlled trials are needed in the future to confirm the clinical effects of these interventions.

Modeling Nutrient Uptake of Cucumber Plant Based on EC and Nutrient Solution Uptake in Closed Perlite Culture (순환식 펄라이트재배에서 EC와 양액흡수량을 이용한 오이 양분흡수 모델링)

  • 김형준;우영회;김완순;조삼증;남윤일
    • Proceedings of the Korean Society for Bio-Environment Control Conference
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    • 2001.04b
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    • pp.75-76
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    • 2001
  • 순환식 펄라이트재배에서 배액 재사용을 위한 양분흡수 모델링을 작성하고자 EC 처리(1.5, 1.8, 2.1, 2.4, 2.7 dSㆍm-1)를 수행하였다. 생육 중기까지 EC 수준에 따른 양액흡수량은 차이가 없었지만 중기 이후 EC가 높을수록 흡수량이 감소되는 경항을 보였다(Fig. 1). NO$_3$-N, P 및 K의 흡수량은 생육기간 동안 처리간 차이를 유지하였는데 N과 K는 생육 중기 이후 일정 수준을 유지하였으나 P는 생육기간 동안 다소 증가되는 경향을 보였다. S의 흡수량은 생육 중기 이후 모든 처리에서 급격한 감소를 보였으며 생육 후기에는 처리간에 차이가 없었다(Fig. 2). 오이의 무기이온 흡수율에서와 같이 흡수량에서도 EC간 차이를 보여 EC를 무기이온 흡수량을 추정하는 요소로 이용할 수 있을 것으로 생각되었다. 무기이온 흡수량은 모든 EC 처리간에 생육 초기에는 차이를 보이지 않았으나 생육중기 이후에는 뚜렷한 차이를 보인 후 생육 후기의 높은 농도에서 그 차이가 다소 감소되는 경향을 보였다. 단위일사량에 따른 양액흡수량과 EC를 주된 변수로 한 오이의 이온 흡수량 예측 회귀식을 작성하였는데 모든 무기이온 흡수량 추정식의 상관계수는 S를 제외한 모든 이온에서 높게 나타났는데 특히 N, P, K 및 Ca에서 높았다. S이온에서의 상관계수는 0.47로 낮게 나타났으나 각 이온들의 회귀식에 대한 상관계수는 모두 1% 수준에서 유의성을 보여 위의 모델식을 순환식 양액재배에서 무기이온 추정식으로 사용이 가능할 것으로 생각되었다(Table 1). 이를 이용한 실측치와의 비교는 신뢰구간 1%내에서 높은 정의상관을 보여 실제적인 적용이 가능할 것으로 생각되었다(Fig 3)..ble 3D)를 바탕으로 MPEG-4 시스템의 특징들을 수용하여 구성되고 BIFS와 일대일로 대응된다. 반면에 XMT-0는 멀티미디어 문서를 웹문서로 표현하는 SMIL 2.0 을 그 기반으로 하였기에 MPEG-4 시스템의 특징보다는 컨텐츠를 저작하는 제작자의 초점에 맞추어 개발된 형태이다. XMT를 이용하여 컨텐츠를 저작하기 위해서는 사용자 인터페이스를 통해 입력되는 저작 정보들을 손쉽게 저장하고 조작할 수 있으며, 또한 XMT 파일 형태로 출력하기 위한 API 가 필요하다. 이에, 본 논문에서는 XMT 형태의 중간 자료형으로의 저장 및 조작을 위하여 XML 에서 표준 인터페이스로 사용하고 있는 DOM(Document Object Model)을 기반으로 하여 XMT 문법에 적합하게 API를 정의하였으며, 또한, XMT 파일을 생성하기 위한 API를 구현하였다. 본 논문에서 제공된 API는 객체기반 제작/편집 도구에 응용되어 다양한 멀티미디어 컨텐츠 제작에 사용되었다.x factorization (NMF), generative topographic mapping (GTM)의 구조와 학습 및 추론알고리즘을소개하고 이를 DNA칩 데이터 분석 평가 대회인 CAMDA-2000과 CAMDA-2001에서 사용된cancer diagnosis 문제와 gene-drug dependency analysis 문제에 적용한 결과를 살펴본다.0$\mu$M이 적당하며, 초기배발달을 유기할 때의 효과적인 cysteamine의 농도는 25~50$\mu$M인 것으로 판단된다.N)A(N)/N을 제시하였다(A(N)=N에 대한 A값). 위의 실험식을 사용하여 헝가리산 Zempleni 시료(15%

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Development of Robotic System based on RFID Scanning for Efficient Inventory Management of Thick Plates (효율적인 후판 재고관리를 위한 RFID 스캐닝 로봇 시스템 개발)

  • Lee, Kwang-Hyoung;Min, So-Yeon;Lee, Jong-Hee
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.10
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    • pp.1-8
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    • 2016
  • Automation of inventory management in a steel plate factory was a difficult problem unresolved for a long time. And now, it is also necessary to work diligently in the steel industry on efficient inventory management of thick plates. So far, the environmental characteristics of stacked thick plates means it is not easy to apply advanced technology for their automatic identification. In this paper, we propose a thick-plate robotic scanning system based on radio-frequency identification (RFID) that can provide quick and accurate inventory management by acquiring plate information after the scanning automatically recognizes the RFID tags under difficult load conditions. This system is equipped with a crane to move the plates in a pulled-up operation. It is equipped with a plate-only linear dipole antenna only for scanning the position of the plate tag. Only the linear dipole antenna, while moving the x-axis and y-axis information, automatically identifies the tag information attached to the plate. The tag information acquired by the system is used for stockpiling and is managed by steel plate inventory control software. The effectiveness of the proposed system is verified through field performance evaluation. As a result, the recognition rate of the plate tags is 99.9% at a maximum distance of 320 cm. The developed thick-plate antenna showed excellent performance compared to an existing commercial antenna.