• Proceedings of the Korean Society of Applied Pharmacology
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• 1992.05a
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• pp.28-28
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• 1992

브라디키닌은 체내에서 강력한 혈관 확장 작용을 일으키는 autacoid(local hormone)로서 혈압의 항상성 유지, 모세혈관 투과성 증진, 염증 및 통증 반응 등에 관여하고 여러 장관 평활근을 수축시킨다. 또한 septic 혹은 endotoxic shock의 여러 원인 물질로도 생각되어진다. 최근 rhinovirus로 인한 감기의 제증상 원인 물질로도 브라디키닌이 주목을 받고있다. 이와같이 브라디키닌온 다양한 질병에 있어 중요한 원인 물질로 여겨지므로 브라디키닌 길항제들은 한두 질병의 치료제로 개발될 가능성이 높음이 강력히 제시되고 있는 실정이다. 이의 개발을 위해서 브라디키닌 수용체에 대한 연구는 필수적이라고 할 수 있다. 본 연구는 두 부분으로 나누어 진행중인데 첫째, 이제까지 보고된 브라디키닌 길항 물질들은 대부분이 브라디키닌의 특정 아미노산 잔기를 치환시킨 펩타이드 유도체로서 이들을 경구 투여시 peptidase어 의하여 쉽게 분해되고 또한 부분적인 효능제 활성을 갖는 불리한 점을 감안하여, 비펩타이드성 브라디키닌 길항제를 개발할 목적으로 한방 및 민간에서 자주 사용되어온 생약중에 브라디키닌 작용에 선택적 길항효과가 있는 물질을 검색한 바 활성을 보인 황금으로부터 작용 성분을 추적중에 있다. 둘째, 브라디키닌 수용체를 순수하게 분리 정제하기 위한 첫 단계로서 이 수용체의 결합시험(binding assay) 방법을 확립하고 더불어 여러 조직내(흰쥐의 여러 기관, 토끼 및 사람의 신장)의 브라디키닌 수용체의 분포를 파악하는 일이다. 횐쥐 조직의 실험 결과로부터 신장에 브라디키닌 수용체가 많이 분포함을 확인되었고 향후 토끼 신장으로부터 동수용체를 분리하고자 한다. 또한 토끼 신장의 근위세뇨관일차배양세포을 이용하여 브라디키닌의 신장에서의 작용기전도 살펴보고 있다.+}$, $Na^{+}$, NH$_{4}$$^{+}$ 및 H$^{+}$) 수용액 메디움에서의 Cd(II), Mg(II) 및 Zn(II)의 Dowex 1-X8, Cl$^{-}$ 수지에 대한 흡착은 역시 어떤 메디움에서도 Cd(II) 흡착이 제일 크며, 다음이 Zn(II) 이고 착이온을 형성않는 Mg(II)이 제일 작았다. 한편 메디움 종류별 D값의 크기순위는 H$^{+}$>K$^{+}$> $Na^{+}$>NH$_{4}$$^{+}$이였다. 메디움의 종류에 따라 D값의 차이가 나는 것은 금속이온의 착이온 형성과 금속이온의 용액내에서의 이온종의 상태와 관련이 있다고 생각된다. 마. MCl(M:K$^{+}$, $Na^{+}$, NH$_{4}$$^{+}$ 및 H$^{+}$)과 MNO$_{3}$ 용리액에 의한 Cd(II), Mg(II) 및 Zn(II)의 용리는 예상한 바와 같이 MCl에서 작은 Dv 값을 갖는데, 이것은 CdCl$_{4}$$^{2-}$ 착이온을 형성하거나 ZnCl$_{4}$$^{2-}$ , ZnCl$_{3}$$^{-}$같은 이온과 MgCl$^{+}$, MgCl$_{2}$같은 이온종을 형성하기 때문인것 같다. 한편 어떠한 용리액에서던지 NH$_{4}$$^{+}$의 경우

• Journal of the Korean Society of School Health
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• v.12 no.2
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• pp.321-337
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• 1999

The purpose of this study was to obtain basic data for the prevention and control of adolescent back pain through analyzing connections between study environments and physical posture. The subjects were 960 male general high school students in the Chonju area and the data were collected by a self-reported questionnaire from Mar. 17 to Mar. 22, 1999. The collected data were analyzed by a frequency, chi-square and t-test using an SPSS program. The results are as follows; 1) The experience rate of back pain perceived by subjects was 67.5% and by each grade: 1st-27.5%, 2nd-35.4%, 3rd-37.1%. The relationship between grades and the experience of back pain didn't show any significant difference. 2) The causes of back pain perceived by subjects such as 'postures are not good' was 56.7%, 'sitting too much time in a chair' was 39.1%, and 'too severe exercise' was 32.4%. 27.8% had back pain first during the 3rd grade of middle school, and 23.9% had it first during the 1st grade of high school. 3) Intensity of subjects' back pain spread from 'moderate' at 49.6%, to 'severe' at 16.4%. Concerning the frequency of back pain, 58.6% said it was 'irregalar'. 4) Among interventions to deal with back pain: 'move by exercising my back or ask friends to beat my back' was 41.0%, 'just bear it' was 23.1%, and at home 'don't have 'any treatment' was ranked first, at 54.9%. 5) The relationship between subjects' general characteristics and back pain experiences: height (t=-1.99, P=.046), sitting/height (t=-2.61, P=.009), self-perceived condition of health (${\chi}^2=23.530$, P=.000), family history (${\chi}^2=43.903$, P=.000) showed significant difference, but the kinds of transportation, sleeping postures, sleeping method and smoking didn't show significant differences. 6) The relationship between subjects' learning environment and back pain experiences, the height of students' desk and chair showed significant difference (${\chi}^2=23.054$, P=.000), but the sitting time didn't show significant difference. 7) The relationship between the characteristics of subjects' physical postures and back pain experiences: standing postures (${\chi}^2=15.105$, P=.001), and sitting postures (${\chi}^2=20.264$X2, P=.001) showed significant difference, but lifting postures didn't show significant difference.

• Journal of The Korean Astronomical Society
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• v.37 no.1
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• pp.41-53
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• 2004

It has been a big mystery what drives filament eruptions and flares. We have studied in detail an X1.8 flare and its associated filament eruption that occurred in NOAA Active Region 9236 on November 24,2000. For this work we have analyzed high temporal (about 1 minute) and spatial (about 1 arcsec) resolution images taken by Michelson Doppler Imager (MDI) onboard the Solar and Heliospheric Observatory, Hoc centerline and blue wing ($-0.6{\AA}$) images from Big Bear Solar Observatory, and 1600 ${\AA}$ UV images by the Transition Region and Corona Explorer (TRACE). We have found that there were several transient brightenings seen in H$\alpha$ and, more noticeably in TRACE 1600 ${\AA}$ images around the preflare phase. A closer look at the UV brightenings in 1600 ${\AA}$ images reveals that they took place near one end of the erupting filament, and are a kind of jets supplying mass into the transient loops seen in 1600 ${\AA}$. These brightenings were also associated with canceling magnetic features (CMFs) as seen in the MDI magnetograms. The flux variations of these CMFs suggest that the flux cancellation may have been driven by the emergence of the new flux. For this event, we have estimated the ejection speeds of the filament ranging from 10 to 160 km $s^{-1}$ for the first twenty minutes. It is noted that the initiation of the filament eruption (as defined by the rise speed less than 20 km $s^{-1}$) coincided with the preflare activity characterized by UV brightenings and CMFs. The speed of the associated LASCO CME can be well extrapolated from the observed filament speed and its direction is consistent with those of the disturbed UV loops associated with the preflare activity. Supposing the H$\alpha$/UV transient brightenings and the canceling magnetic features are due to magnetic reconnect ion in the low atmosphere, our results may be strong observational evidence supporting that the initiation of the filament eruption and the preflare phase of the associated flare may be physically related to low-atmosphere magnetic reconnection.

• KSBB Journal
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• v.11 no.5
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• pp.606-612
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• 1996

A specific ammino auid in a mixture can be crystallized inside an ion exchange column when displacer concentration is high enough to concentrate the amino acid in a pure band beyond its solubility limit. Glutamic acid formpd a discrete crystal layer in a cation exchanger column by operating displacement development mode and using a high concentration of displacer NaOH. The glutamic acid crystal formed was eluded from the column with the effluent stream and collected in a fraction collector. When 1.0 M of NaOH was used as a displacer, more than 60% of the loaded glutamic acid was recovered as crystal. The continuous crystallization and dissolution of crystal occurred, resulting in apparent movement of the crystal along the column without clogging or pressure increase. NaOH was proved a better displacer than NaCl because hydroxide ions neutralized hydrogen ions released from the resin and thus reduced the number of hydrogen ion competing with sodium ion for re-adsorption. The displacement development process coupled with crystallization provided higher concentration and recovery of glutamic acrid than conventional chromatography.

• The Journal of Korean Academy of Prosthodontics
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• v.28 no.1
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• pp.137-163
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• 1990

The properties of a investment material can be described by the consistency at the slurry state, the setting time, the compressive strength and the thermal expansion during the casting. In this study the effect of the production parameters which are included the ratio of quartz and cristobalite, the content of binder, the water powder ratio and the content and concentration of additives on the Properties of the gypsum-bonded investments has been investigated with help of the consistency test, the vicat needle test, the compressive strength test, the thermal expansion test, x-ray diffraction and DTA thermal differential test. The experimental results showed that the constitution of a investment with W/P ratio of 0.34, 30% of gypsum, 0.8% aluminium sulfate, 2% magnesium sulfate, 0.6% sodium phosphate was adapted for the properties of the KDA Spec. No. 13 type I investment. The important experimental results are summarized as follows. 1. The consistency of the investment decreased with increasing amount of aluminium sulfate and decreasing amount of sodium phosphate. An addition of magnesium sulfate up to 2% an increase of the consistency was shown. But 3% magnesium sulfate in investment showed a decrease of the consistency. The consistency did not vary significantly with a variation of the content of gypsum and cristobalite and the W/P ratio. 2. Aluminium sulfate and the magnesium sulfate promoted the hardening and the aluminium phosphate delayed the hardening. The setting time increased with amount of gypsum. The effect of the matrix on the setting time was insignificant. With the W/P ratio of 0.34 the setting time was 14 min. 3. The compressive strength decreased with the amount of aluminium sulfate up to 0.25% and increased with the amount of aluminium sulfate greater than 3%. The compressive strength decreased as decreasing the amount of magnesium sulfate and gypsum and as increasing the W/P ratio. The effect of the refractory on the compressive strength was also not significant. With the W/P ratio of 0.34 the compressive strength was $34Kg/mm^2$. 4. The 1st thermal expansion was found at the temperature near and the steady state or the contraction stage was found at the temperature between $250^{\circ}C$ and $500^{\circ}C$. After this stage the 2nd thermal expansion took place at the temperature near $500^{\circ}C$. The amount of thermal expansion increased with decreasing the content of magnesium sulfate, aluminium sulfate and gypsum and the W/P ratio. And the amount of thermal expansion increased as the content of sodium phosphate and cristobalite. With the W/P ratio of 0.34 the amount of total expansion was 1.2%.

• Women's Health Nursing
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• v.7 no.4
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• pp.642-656
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• 2001

The purpose of this study is to find the difference of the climacteric symptoms and quality of life according to hormone replacement therapy (HRT) in climacteric women. The research design was a descriptive survey with questionaries. A convenience sample of 181 climacteric women aged 45-65 who had climacteric symptoms were selected in Kwangju city, Korea. Sixty-six climacteric women among those subjects were receiving HRT at K university hospital in Kwangju city. Data were collected by the interview with questionaries from Feb 3. 1999 to March 25, 1999. Climacteric symptoms were measured using scores developed by Neugarten(1965) and modified by Park(1989), and the quality of life scale related to climacteric symptoms developed by Hildtich(1996) and modified Kim(1998). Data were analyzed by $x^2$-test, t-test, ANOVA and Pearson's correlation procedures, using SAS/win PC+. The results were as follows ; 1. No statistically significant differences were found between the receiving HRT group and not receiving HRT group in demographic, obstetrical and health related characteristics. 2. There were no significant difference on the climacteric symptoms between the group of women receiving HRT (score 2.4) and the group of women who were not (score 2.6) except psychological area (t= 2.407, p= 0.017). 3. The quality of life of the group of women receiving HRT was significantly higher than that the group of women who were not (t=2.151, p=.032). 4. The quality of life of the group of women receiving HRT were high scores There were significantly high of the quality of life in the vaso-motor change area (t=2.634, p= 0.009), psycho-social change area (t=3.239, p=0.001), and physical change area (t=2.031, p=0,043) in the group of receiving HRT compared to not receiving HRT group. 5. The variables showed significantly differences on the degree of climacteric symptoms of the group of women not receiving HRT were the subjective health state (t=15.81, p=0.000), age (t=7.50, p=0.007), feeling of menopause (t=30.88, p=0,000) and climacteric periods (t=8.66, p=0.003), and receiving HRT were number of para (t=3.95, p=0.050) and feeling of menopause (t=3.94, p=0.050). 6. The variables showed significantly difference on the quality of life of the group of women not receiving HRT were the subjective health state (t=4.14, p=0.044) and feeling of menopause (t=10.86, p=0.001). 7. There were significantly positive correlations between the climacteric symptoms and the quality of life in climacteric women (r=0.512, p=0.000).

• Asian-Australasian Journal of Animal Sciences
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• v.11 no.6
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• pp.692-696
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• 1998

An experiment was conducted with eight growing sheep (average initial weight 20.6 kg and average final weight 23.7 kg) in a $4{\times}4$ Latin square design to study the effect of type of forage supplementation to a basal diet of rice straw (ad libitum) and cassava (Manihot esculanta, approximately 9 g of dry matter $(DM).kg^{-0.75}{\cdot}day^{-1}$) on voluntary intake, digestion, rumen microbial protein synthesis and daily weight gain. Forages used were Leucaena (L, Leucaena leucocephala), Gliricidia (G, Gliricidia maculata) and Tithonia (T, Tithonia diversifolia, wild sunflower) at a DM supplementation level of approximately $13g.kg^{-0.75}.day^{-1}$. Organic matter intake was 40.4, 55.5, 55.0 and $54.9g{\cdot}kg.^{-0.75}{\cdot}day^{-1}$ for control (C, ad libitum straw and cassava), L, G and T. respectively, significantly lower for C than for the supplemented diets. Intake of supplementary forage had also a significantly positive effect on voluntary rice straw intake. All forage supplemented diets showed a significantly higher whole diet organic matter digestion than C ($488g{\cdot}kg^{-1}$), while T ($557g{\cdot}kg^{-1}$) differed significantly from L ($516g{\cdot}kg^{-1}$) but not from G ($526g{\cdot}kg^{-1}$). Daily weight gain was -1.7, 5.2, 5.4 and $4.7g{\cdot}kg^{-0.75}$, for C, L, G and T. respectively, significantly lower for C than for the forage-supplemented diets. Efficiency of microbial protein synthesis estimated from urinary excretion of purine derivatives was lower for C (3.8 g microbial N. (kg digestible organic matter intake $(DOMI))^{-1}$ than for the forage supplemented diets (11.3, 9.0 and 9.4 g microbial $N.(kg\;DOMI)^{-1}$ for L, G and T. respectively).

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.2
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• pp.263-271
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• 2010

This trial was conducted to determine the effects of feeding a diet containing solid-state fermented rapeseed meal on performance, nutrient digestibility, intestinal ecology and intestinal morphology of broiler chickens. A mixed liquid culture, containing approximately 5 log cfu/ml Lactobacillus fermentum, Enterococcus faecium, Saccharomyces cerevisae and Bacillus subtilis was prepared in a 1:1:1:1 ratio. A basal substrate (BS) containing 75% rapeseed, 24% wheat bran and 1% brown sugar was mixed with the liquid culture in a ratio of 10:3. Over the 30-day fermentation, isothiocyanates were reduced from 119.6 to 14.7 mmol/kg. A total of 168, day-old male Arbor Acres broiler chicks were assigned to one of three dietary treatments including a corn-soybean meal based control diet as well as two experimental diets in which the control diet was supplemented with 10% of the BS containing unfermented rapeseed meal or 10% of the BS containing rapeseed meal subjected to solid state fermentation. There were 8 pens per treatment and 7 birds per pen. From days 19-21 and days 40-42, uncontaminated excreta were collected from each pen for digestibility determinations. In addition, digesta from the colon and ceca were collected to determine the number of lactobacilli, enterobacteria and total aerobes. The middle sections of the duodenum, jejunum, and ileum were collected for intestinal morphology. Over the entire experimental period (d 1-42), the weight gain and feed conversion of birds fed fermented rapeseed meal were superior (p<0.05) to that of birds fed nonfermented rapeseed meal and did not differ from the soybean control. On day 42, birds fed fermented rapeseed meal had higher (p<0.05) total tract apparent digestibility coefficients for dry matter, energy, and calcium than birds fed non-fermented rapeseed meal. Colon and ceca digesta from broilers fed the fermented feed had higher (p<0.05) lactobacilli counts than birds fed the control and non-fermented rapeseed meal diets on day 21 and 42. Fermentation also improved (p<0.05) villus height and the villus height:crypt depth ratio in the ileum and jejunum on day 21 and 42. The results indicate that solid-state fermentation of rapeseed meal enhanced performance and improved the intestinal morphology of broilers and may allow greater quantities of rapeseed meal to be fed to broilers potentially reducing the cost of broiler production.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.8
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• pp.1169-1177
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• 2012

An experiment was conducted to determine the effects of different amounts of dietary linoleic acid (LA) on growth performance, serum biochemical traits, meat quality, fatty acids composition of muscle and liver, acetyl-CoA carboxylase (ACC) and carnitine palmitoyl transferase 1 (CPT 1) mRNA expression in the liver of 9 wks old to 13 wks old growing meat rabbits. One hundred and fifty 9 wks old meat rabbits were allocated to individual cages and randomly divided into five groups. Animals in each group were fed with a diet with the following LA addition concentrations: 0, 3, 6, 9 and 12 g/kg diet (as-fed basis) and LA concentrations were 0.84, 1.21, 1.34, 1.61 and 1.80% in the diet, respectively. The results showed as follows: the dietary LA levels significantly affected muscle color of LL included $a^*$ and $b^*$ of experimental rabbits (p<0.05). The linear effect of LA on serum high density lipoprotein cholesterol was obtained (p = 0.0119). The saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs) contents of LL decreased and the polyunsaturated fatty acids (PUFAs) content of LL increased with dietary LA increase (p<0.0001). The PUFA n-6 content and PUFA n-3 content in the LL was significantly affected by the dietary LA levels (p<0.01, p<0.05). The MUFAs content in the liver decreased and the PUFAs contents in the liver increased with dietary LA increase (p<0.0001). The PUFA n-6 content and the PUFA n-6/n-3 ratio in the liver increased and PUFA n-3 content in the liver decreased with dietary LA increase (p<0.01). The linear effect of LA on CPT 1 mRNA expression in the liver was obtained (p = 0.0081). In summary, dietary LA addition had significant effects on liver and muscle fatty acid composition (increased PUFAs) of 9 wks old to 13 wks old growing meat rabbits, but had little effects on growth performance, meat physical traits and mRNA expression of liver relative enzyme of experimental rabbits.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.6
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• pp.856-863
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• 2013

An in vitro gas production technique was used in this study to elucidate the effect of two strains of active live yeast on methane ($CH_4$) production in the large intestinal content of pigs to provide an insight to whether active live yeast could suppress $CH_4$ production in the hindgut of pigs. Treatments used in this study include blank (no substrate and no live yeast cells), control (no live yeast cells) and yeast (YST) supplementation groups (supplemented with live yeast cells, YST1 or YST2). The yeast cultures contained $1.8{\times}10^{10}$ cells per g, which were added at the rates of 0.2 mg and 0.4 mg per ml of the fermented inoculum. Large intestinal contents were collected from 2 Duroc${\times}$Landrace${\times}$Yorkshire pigs, mixed with a phosphate buffer (1:2), and incubated anaerobically at $39^{\circ}C$ for 24 h using 500 mg substrate (dry matter (DM) basis). Total gas and $CH_4$ production decreased (p<0.05) with supplementation of yeast. The methane production reduction potential (MRP) was calculated by assuming net methane concentration for the control as 100%. The MRP of yeast 2 was more than 25%. Compared with the control group, in vitro DM digestibility (IVDMD) and total volatile fatty acids (VFA) concentration increased (p<0.05) in 0.4 mg/ml YST1 and 0.2 mg/ml YST2 supplementation groups. Proportion of propionate, butyrate and valerate increased (p<0.05), but that of acetate decreased (p<0.05), which led to a decreased (p<0.05) acetate: propionate (A: P) ratio in the both YST2 treatments and the 0.4 mg/ml YST 1 supplementation groups. Hydrogen recovery decreased (p<0.05) with yeast supplementation. Quantity of methanogenic archaea per milliliter of inoculum decreased (p<0.05) with yeast supplementation after 24 h of incubation. Our results suggest that live yeast cells suppressed in vitro $CH_4$ production when inoculated into the large intestinal contents of pigs and shifted the fermentation pattern to favor propionate production together with an increased population of acetogenic bacteria, both of which serve as a competitive pathway for the available H2 resulting in the reduction of methanogenic archaea.