• Food Science and Biotechnology
• /
• v.16 no.1
• /
• pp.104-109
• /
• 2007

A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect three varieties of genetically modified (GM) canola. The construct-specific primers were used to distinguish the following three varieties of GM canola; GT73, MS8xRF3, and T45, using multiplex PCR. The FatA (fatty acyl-ACP thioesterase) gene was used as an endogenous canola reference gene in the PCR detection. The primer pair Canendo-FIR containing a 105 bp amplicon was used to amplify the FatA gene and no amplified product was observed in any of the 15 different plants used as templates. The GT73-KHUF1/R1 primer recognized the 3'-flanking region of GT73, resulting in an amplicon of 125 bp. The Barstar-F1/MS8xRF3-R primer recognized the junction region of bars tar and the NOS terminator introduced into MS8xRF3, resulting in a 162 bp amplicon, and the T45-F2/R2 primer recognized the junction region of PAT and the 35S terminator introduced into T45, resulting in an amplicon of 186 bp. This multiplex PCR allowed for the detection of construct-specific targets in a genomic DNA mixture of up to 1% GM canola containing GT73, MS8xRF3, and T45.

• Journal of the Korean Ceramic Society
• /
• v.40 no.5
• /
• pp.455-459
• /
• 2003

Tunneling spectra of B $i_2$S $r_2$Ca(C $u_{1-x}$ N $i_{x}$ )$_2$ $O_{8+}$$\delta$/ film by LPE method have been measured using break junctions. The energy gap 2$\Delta$ and 2$\Delta$/ $k_{B}$ $T_{c}$ $^{zero}$ increased with increase of ft. We obtained the energy gap Parameter 2$\Delta$(4.2 K) = 54.4~64 meV, and corresponding1y $\Delta$/ $k_{B}$ $T_{c}$ $^{zero}$=7.36~10.14, larger than the BCS value. The lattice constant c and critical temperature $T_{c}$ $^{zero}$ decrease with increase of $\chi$$_{L}$.

• Journal of the Korean Chemical Society
• /
• v.42 no.6
• /
• pp.652-656
• /
• 1998

$(Ba_{0.8-x}Sr_{0.2})Y_xTiO_3\;(BSYT)$ powders were prepared by the calcination of metal-oxalate precipitates, which were obtained by wet chemical method using Ba, Sr, Y and Ti-nitrates and oxalic acid. Yttrium content, sintering temperature and cooling rate were taken as experimental factors. Response surface method was applied to modelling of the room temperature resistivity of BSYT. The results indicated that the Yttrium content had larger effect on the room temperature resistivity and minimum room temperature resistivity was at Yttrium content of 0.24 mol%. The validity of a modelling equation was confirmed by comparing the measured room temperature resistivity with the calculated one.

• Korean Journal of Materials Research
• /
• v.2 no.2
• /
• pp.151-156
• /
• 1992

Using $Ba(OH)_2{\cdot}8H_2O, \;Sr(OH)_2{\cdot}8H_2O$ and $Ti(i-OC_3H_7)_4$, fine $(Ba_{1-x}, \;Sr_{x})TiO_3$ powders were synthesized through sol-gel process. The particle size of the powders calcined at $700^{\cric}C$ proved to be 20-40nm by the observation of TEM micrographs and measurement of BET specific surface area. The analysis of XRD patterns showed that the phase of the powders was cubic, and it was identified with the lattice parameters determined through XRD patterns and the shift of (112) peaks that the solid solution powders were synthesized. It was expected through the analysis of relative ratio of cations and the uniformity of compositions in the powders examined by EDAX analysis and relative dielectric constant measurements for sintered body that the distribution of cations was uniform in particle unit.

• Journal of Sensor Science and Technology
• /
• v.7 no.2
• /
• pp.103-110
• /
• 1998

Pyroelectric infrared detectors based on La-modified $PbTiO_{3}$ (PLT) thin films have been fabricated by RF magnetron sputtering and rnicrornachining technology. The detectors form $Pb_{l-x}La_{x}Ti_{1-x/4}O_{3}$ (x=0.05) thin film ferroelectric capacitors epitaxially grown by RF magnetron sputtering on Pt/MgO (100) substrate. The sputtered PLT thin film exhibits highly c-axis oriented crystal structure that no poling treatment for sensing applications is required. This is an essential factor to increase the yield for realization of an infrared image sensor. Micromachining technology is used to lower the thermal mass of the detector by giving maximum sensor efficiency. Polymide is coated on top of the sensing elements to support the fragile structure and the backside of the MgO substrate is selectively etched to reduce the heat loss. The sensing element exhibited a very high detectivity D* of $8.5{\times}10^{8}cm{\cdot}\sqrt{Hz}/W$ at room temperature and it is about 100 times higher than the case of micromachining technology is not used. A sensing system that detects the position as well as the existence of a human body is realized using the array sensor.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.13 no.1
• /
• pp.95-107
• /
• 1985

This study is purposed to investigate the role of grasses as the Green Foundation effect on the visual preference to flowering tree and shrub being the principal elements of natural landscape early in the spring. As the flowering shrub materials, Rhododendron mucronulatum and Forsythia Kreana were adopted. Total 48 slides were photographed at the 8 different lawn areas with the 6 planting combinations of flowering shrub materials, and 10 landscape variables - dimensional and color - were measured and preference scores were taken by slide evaluations. The results were : 1) The visual preference to the landscape of flowering shrub in the lawn area was changed with the different lawn situations. 2) With important 4 variables, multilinear regression model was established, hence Y =40.4 + 9.6($X_1$) -7.8($X_2$) -26.8($X_3$) + 15.2($X_4$) where, Y : estimated preference score $X_1$: perimeter of flower zone $X_2$: value of green covered zone $X_3$: hue of green covered zone $X_4$: chroma of green covered zone 3) Most effective variable was 'hue of green covered zone', hence the more green the lawn area ism the more preferred landscape or the more effective green foundation is.

• Asian-Australasian Journal of Animal Sciences
• /
• v.2 no.2
• /
• pp.59-66
• /
• 1989

Growing and finishing Korean native bulls were used to investigate the effects of different dietary energy levels on performance and energy intake. Experimental observations were made over three weight categories (200 to 250 kg, 250 to 350 kg and 350 to 450 kg). Three diets (2.4, 2.6 and 2.8 Mcal ME/kg DM) were used for each weight category. Crude protein levels of the diets were 12, 11 and 10% for the respective weight groups. Dietary energy level did not significantly affect daily body gain within a weight range but daily body gain during the entire experiment was higher (P<.05) in bulls receiving 2.6 Mcal energy diet than in those fed 2.4 and 2.8 Mcal energy diets. The following equation was developed to predict daily body gain(Y) from energy levels(X) of ration. $Y\;=\;1.3.475X\;-\;2.5949X^{2}\;-\;16.355$ Increasing energy levels significantly (P<.05) decreased daily feed intake. The following equation was developed to predict daily feed intake(Y) from energy levels(X) of ration. $Y\;=\;-30.013X\;+\;5.4401X^{2}\;+\;49.119$ Feed intake per metabolic body size during the entire feeding period ranged from 100.9 to 110.8 g and was lower in bulls fed 2.6 and 2.8 Mcal energy diets than in those fed 2.4 Mcal energy diet. Increasing energy levels significantly (P<.05) improved feed efficiency. The following equation was developed to predict feed efficiency(Y) from energy levels(X) of ration. $Y\;=\;-118.34X\;+\;22.448X^{2}\;+\;162.85$ Daily energy intake during the entire experiment ranged from 18.90 to 19.99 Mcal and there was no significant difference among energy levels. Daily energy intake per metabolic body size during the feeding period ranged from 248.6 to 260.8 kcal and was slightly higher in bulls receiving 2.8 Mcal than in those fed 2.4 and 2.6 Mcal energy diets. Energy required per kg body gain ranged from 17.25 to 19.11 Mcal and was slightly lower in bulls receiving 2.6 Mcal energy diet than in those fed 2.4 and 2.8 Mcal energy diets.

• Journal of the Korean Crystal Growth and Crystal Technology
• /
• v.9 no.2
• /
• pp.245-250
• /
• 1999

The effect of cation substitution on the inonic conductivity was studied in $(Li_{0.5}La_{0.5})_{1-y}Sr_yTi_{1-x}Mn_xO_3$ system. In case of Sr substitution, the ionic conductivity abrubtly decreased over y=0.05. This may be caused by the decrease of lithium contents which contribute to lithium inonic conductivity. Jahn-Teller distortion as well as lattice parameter variation influenced the inonic conductivity in case of Mn substitution. A and B-site cations effects on the conductivities were found to be independent, and the ionic conductivity as high as $2.8{\times}10^{-2}$S/cm was obtained in x=0.0006 and y=0.05.

• Korean Journal of Materials Research
• /
• v.7 no.10
• /
• pp.898-907
• /
• 1997

Fe-22Cr-5AI-X(X=Zr, Y)합금을 1143K, 고온 황화(P$s_{2}$=1.11x$10^{-7}$atm, P$O_{s}$ =3.11x$10^{-20atm}$) 및 황화/산화 (P$s_{2}$=8.31x$10^{-8}$atm, P$O_{s}$ =3.31x$10^{-18atm}$) 환경의 복합가스 분위기에서 1-30시간동안 노출하여 합금표면에 형성된 부식층을 관찰하여 SEM/EDS로 분석하였다. Fe-22Cr-5AI합금은 고온 부식환경에서 부식 생성물의 성장은 포물선법칙을 따르고 주요 성분은 결함이 많고 다공질인 철과 크롬의 황화물[(Fe, Cr)Sx]이므로 고온 내식성이 감소하였다. Zr을 1wt%첨가한 Fe-22Cr-5AI합금의 고온 부식거동은 Y을 1wt%첨가한 합금과 비슷한 거동을 나타내었다. 황화환경에서는 Cr의 선택 황화에 의한 크롬 황화물(CrS)이 생성되고 노출시간의 경과에 따라 (Fe, Cr)Sx나 (Cr, Fe)Sx 등의 황화물의 성장으로 고온 내식성이 감소하였다. 그러나 황화/산화환경에서는 초기에는 알루미늄산화물(A $I_{2}$ $O_{3}$)및 지르코늄산화물(Zr $O_{2}$)등이 생성되어 보호적이었으나 15시간이후 부터 (Fe, Cr)Sx나 (Cr, Fe)Sx 의 황화물의 성장으로 고온 내식성이 감소하였다.

• Korean Journal of Crystallography
• /
• v.12 no.4
• /
• pp.233-238
• /
• 2001

X-ray microdiffraction which uses x-ray beam focused down to a micron size from synchrotron radiation sources allow precision measurements of local orientation and strain variations in polycrystalline materials. Using x-ray microdiffraction setup at Pohang Light Source, we investigated the tex-ture of Cu interconnects with various widths on Si wafer by collecting Laue images and focused to about 2×3㎛ ² in size. Our results show that 1㎛ wide Cu interconnect had grains in rather ran- dom orientation. On the other hand the 20㎛ wide interconnects showed a 〈111〉fiber texture near the center. The grains were 2∼5㎛ long at the 1㎛ wide interconnect and 6∼8㎛ in size at the 20㎛ wide interconnect.