• Experimental and Molecular Medicine
• /
• v.50 no.12
• /
• pp.1.1-1.14
• /
• 2018

DNA methylation is a regulatory mechanism in epigenetics that is frequently altered during human carcinogenesis. To detect critical methylation events associated with gastric cancer (GC), we compared three DNA methylomes from gastric mucosa (GM), intestinal metaplasia (IM), and gastric tumor (GT) cells that were microscopically dissected from an intestinal-type early gastric cancer (EGC) using methylated DNA binding domain sequencing (MBD-seq) and reduced representation bisulfite sequencing (RRBS) analysis. In this study, we focused on differentially methylated promoters (DMPs) that could be directly associated with gene expression. We detected 2,761 and 677 DMPs between the GT and GM by MBD-seq and RRBS, respectively, and for a total of 3,035 DMPs. Then, 514 (17%) of all DMPs were detected in the IM genome, which is a precancer of GC, supporting that some DMPs might represent an early event in gastric carcinogenesis. A pathway analysis of all DMPs demonstrated that 59 G protein-coupled receptor (GPCR) genes linked to the hypermethylated DMPs were significantly enriched in a neuroactive ligand-receptor interaction pathway. Furthermore, among the 59 GPCRs, six GI hormone receptor genes (NPY1R, PPYR1, PTGDR, PTGER2, PTGER3, and SSTR2) that play an inhibitory role in the secretion of gastrin or gastric acid were selected and validated as potential biomarkers for the diagnosis or prognosis of GC patients in two cohorts. These data suggest that the loss of function of gastrointestinal (GI) hormone receptors by promoter methylation may lead to gastric carcinogenesis because gastrin and gastric acid have been known to play a role in cell differentiation and carcinogenesis in the GI tract.

• Journal of The Korea Institute of Healthcare Architecture
• /
• v.27 no.3
• /
• pp.71-78
• /
• 2021

Purpose: It is important to plan the ward module at a time when the size of beds, the floor area, and the construction budget are all set prior to the hospital design. In this context this study aims (1) to derive various factors affecting the ward module, and (2) to analyze the appropriate room module according to the type. Methods: Design factors related to hospital modules are derived through precedential studies, and the types of ward elevation are classified by reviewing the drawings of 18 case hospitals. And the detailed dimensions and area of the derived elements are analyzed. Results: The X-axis modules of the ward are switched to long span structural columns of 9.9 m, 12.6 m and 13.2 m, but the ward modules still represent 6.6 m. The Y-axis module of the ward shows a dimension of 9 to 9.9m in the process of changing a multi-person room into a four-person room. Type A of curtain wall with columns located on the wall of the room and type B of curtain wall located in the center of the room are analyzed due to their variations. The square window type, which forms the elevation of the square window by exposing the columns to the elevation, and the outframe type, which protrudes from the structural columns and beams, have elevation designs limited. There are, however, no obstacles to the interior space of the hospital room, so the wall composition and furniture arrangement are expected to be free. The ward area of Curtain Wall Type A, which can secure an effective area of 5.9m*5.0m, are 52.1m². The Curtain Wall Type A, Square window type, and the outframe type are 49.8m². Implications: As part of the hospital standard module plan for economical and reasonable hospital building planning, a type was proposed in this study in conjunction with the external design. It is hoped that it be a base for standard module research linked together to the Central Treatment department, Outpatient department and underground parking lot.

• Journal of Cadastre & Land InformatiX
• /
• v.48 no.2
• /
• pp.153-170
• /
• 2018

The purpose of this study is to diagnose problems by analyzing the status of system and user in order to activate the Urban Planning Information System(UPIS). The UPIS has been distributed to all local governments through pilot and expansion projects since 2003 to utilize spatial information in urban planning and administration. The original purpose was to use GIS to support planning and to support scientific decision making, but now the main task is to designate and manage zoning districts related to land use. Therefore, the UPIS is responsible for the production (preparation procedure) and management of the local district information for use in the Korea land use information system, which is an upper concept defined by the law, but it is evaluated that its identity is ambiguous and utilization rate is low. This study is conducted to investigate the four aspects (business process, database, system, citizen service) of UPIS, and suggest concrete improvement measures. To summarize, each individual system exists in the Korea land use information system, but they all share similar data and have a linked work flow. Therefore, we suggested that the DB current plan should be established to improve the reliability of data and to redefine the business process around the UPIS.

• The Journal of Korea Assosiation for Disability and Oral Health
• /
• v.15 no.1
• /
• pp.35-39
• /
• 2019

Oculo-cerebro-renal syndrome, known as Lowe syndrome, is an X-linked gene disorder characterized by congenital cataracts, brain abnormalities and renal dysfunction. Tooth crowding, taurodontism, delayed eruption in the permanent dentition and over-retained primary teeth with ectopic eruption of the permanent teeth have been reported as dental findings. Because of the high incidence of poor cooperation, patients with Lowe syndrome have difficulties in maintaining good oral hygiene, which may require dental treatment. We present a case of dental treatment for the uncooperative child with Lowe syndrome under general anesthesia. A 4-year-old, 11.2 kg boy with Lowe syndrome visited Seoul National University Dental Hospital for gingival swelling. The patient had multiple caries requiring dental treatment. Because of his past history of malignant hyperthermia during inhalation induction, anesthesia was induced and maintained with total intravenous anesthesia (TIVA) after medical consultation. Dental restorative treatments were successfully performed and no complications were observed during and after the procedure. Safe and effective dental management of the patients with Lowe syndrome could be performed with the help of general anesthesia and careful monitoring.

• The Journal of Korea Assosiation for Disability and Oral Health
• /
• v.14 no.2
• /
• pp.97-101
• /
• 2018

Lesch-Nyhan syndrome is a rare X-linked recessive genetic disorder. During purine metabolism, the hypoxanthine guanine phosphribosyl transferase (HGPRT) enzyme is deficient causing phosphoribosyl transferase to accumulate and resulting in excessive uric acid. Clinical symptoms include hypercalcemia, choreoathetosis, spasticity, mental retardation, and self-injury to lips, tongue or fingers. This results not only in pain caused by the self-injury but also secondary infection of the wound site and the esthetic damage of the soft tissue defect. Dental treatments include conservative methods using intraoral appliances such as soft mouthguards, fixed lip bumpers, and occlusal guards, and invasive methods such as extraction of all teeth or forming an artificial anterior open bite. We report two cases of Lesch-Nyhan syndrome patients with self-mutilation behavior; one was treated with a preservative method using a soft mouthguard, and the other was treated with extraction of all teeth.

• Journal of Ginseng Research
• /
• v.45 no.2
• /
• pp.228-235
• /
• 2021

Backgroud: Ginsenoside compound K (GK) is a major metabolite of protopanaxadiol-type ginsenosides and has remarkable anticancer activities in vitro and in vivo. This work used an ionic cross-linking method to entrap GK within O-carboxymethyl chitosan (OCMC) nanoparticles (Nps) to form GK-loaded OCMC Nps (GK-OCMC Nps), which enhance the aqueous solubility and stability of GK. Methods: The GK-OCMC Nps were characterized using several physicochemical techniques, including x-ray diffraction, transmission electron microscopy, zeta potential analysis, and particle size analysis via dynamic light scattering. GK was released from GK-OCMC Nps and was conducted using the dialysis bag diffusion method. The effects of GK and GK-OCMC Nps on PC3 cell viability were measured by using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay. Fluorescent technology based on Cy5.5-labeled probes was used to explore the cellular uptake of GK-OCMC Nps. Results: The GK-OCMC NPs had a suitable particle size and zeta potential; they were spherical with good dispersion. In vitro drug release from GK-OCMC NPs was pH dependent. Moreover, the in vitro cytotoxicity study and cellular uptake assays indicated that the GK-OCMC Nps significantly enhanced the cytotoxicity and cellular uptake of GK toward the PC3 cells. GK-OCMC Nps also significantly promoted the activities of both caspase-3 and caspase-9. Conclusion: GK-OCMC Nps are potential nanocarriers for delivering hydrophobic drugs, thereby enhancing water solubility and permeability and improving the antiproliferative effects of GK.

• Nutrition Research and Practice
• /
• v.16 no.6
• /
• pp.685-699
• /
• 2022

BACKGROUND/OBJECTIVES: Platycodon grandiflorum (PG) has long been known as a medicinal herb effective in various diseases, including bronchitis and asthma, but is still more widely used for food. Fermentation methods are being applied to increase the pharmacological composition of PG extracts and commercialize them with high added value. This study examines the hydrolyzed and fermented PG extract (HFPGE) fermented with Lactobacillus casei in RAW 264.7 cells, and investigates the effect of amplifying the immune and the probable molecular mechanism. MATERIALS/METHODS: HFPGE's total phenolic, flavonoid, saponin, and platycodin D contents were analyzed by colorimetric analysis or high-performance liquid chromatography. Cell viability was measured by the MTT assay. Phagocytic activity was analyzed by a phagocytosis assay kit, nitric oxide (NO) production by a Griess reagent system, and cytokines by enzyme-linked immunosorbent assay kits. The mRNA expressions of inducible nitric oxide synthase (iNOS) and cytokines were analyzed by reverse transcription-polymerase chain reaction, whereas MAPK and nuclear factor (NF)-κB activation were analyzed by Western blots. RESULTS: Compared to PGE, HFPGE was determined to contain 13.76 times and 6.69 times higher contents of crude saponin and platycodin D, respectively. HFPGE promoted cell proliferation and phagocytosis in RAW 264.7 cells and regulated the NO production and iNOS expression. Treatment with HFPGE also resulted in increased production of interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, C-X-C motif chemokine ligand10, granulocyte-colony-stimulating factor, granulocyte-macrophage colony-stimulating factor, and monocyte chemoattractant protein-1, and the mRNA expressions of these cytokines. HFPGE also resulted in significantly increasing the phosphorylation of NF-κB p65, extracellular signal-regulated kinase, and c-Jun N-terminal kinase. CONCLUSIONS: Taken together, our results imply that fermentation and hydrolysis result in the extraction of more active ingredients of PG. Furthermore, we determined that HFPGE exerts immunostimulatory activity via the MAPK and NF-κB signaling pathways.

• Journal of Cadastre & Land InformatiX
• /
• v.53 no.1
• /
• pp.37-46
• /
• 2023

As real estate values rise, interest in cadastral resurvey is increasing. Accordingly, a cadastral resurvey project is actively underway for drone operation through securing work efficiency and improving accuracy. The need for utilization and management of cadastral resurvey results (drone images) is being raised, and through this study, a 3D spatial information platform was developed to solve the existing drone image management and utilization limitations and to provide drone image-based 3D cadastral information. It is proposed to build and use. The study area was selected as a district that completed the latest cadastral resurvey project in which the study was organized in February 2023. Afterwards, a web-based 3D platform was applied to the study to solve the user's spatial limitations, and the platform was designed and implemented based on drone images, spatial information, and attribute information. Major functions such as visualization of cadastral resurvey results based on 3D information and comparison of performance between previous cadastral maps and final cadastral maps were implemented. Through the open platform established in this study, anyone can easily utilize the cadastral resurvey results, and it is expected to utilize and share systematic cadastral resurvey results based on 3-dimensional information that reflects the actual business district. In addition, a continuous management plan was proposed by integrating the distributed results into one platform. It is expected that the usability of the 3D platform will be further improved if a platform is established for the whole country in the future and a service linked to the cadastral resurvey administrative system is established.

• Maritime Security
• /
• v.6 no.1
• /
• pp.1-35
• /
• 2023

The purpose of this study is to present the direction of development of our navy's response strategy through analysis at the ends, ways, and means level of the Chinese navy's offensive strategy for the West Sea. As a result of the analysis, at the ends level, the Chinese Navy's offensive strategy for the West Sea strategy is being linked to a grand strategy to protect maritime rights and achieve maritime power between the U.S. and China competition, at the ways level, the Chinese Navy is expected to create a foundation for the international community to recognize the West Sea as China's inland sea through "routine entry" and "exercise authority", and in case of emergency, it will try to secure sea control in the West Sea in a short period of time by blocking Korea's maritime transportation route based on the overwhelming preemptive attack capability of aircraft carriers. At the means level, it is accelerating the construction of aircraft carrier warfare units and improving its ability to engage long-range missiles. As a direction of development of the Korean Navy's response strategy in response to this, first, Establishment and Development of National Maritime Security Strategy in conjunction with the Korean Indo-Pacific Strategy. Second, it proposes the development of the concept of effective security operations for the east sea area of the West Sea intermediate line, and third, the development of the concept of combat performance and capacity building to strengthen survival and lethality.

• Journal of Cadastre & Land InformatiX
• /
• v.53 no.2
• /
• pp.107-122
• /
• 2023

The demand for spatial information in the era of the 4th Industrial Revolution is expanding Additionally, interest in attribute data related to geography or location is increasing. In the field of spatial information, spatial information policies and services tailored to the public can be provided through linkage and integration with new attribute data, and these data are resources for this purpose. In order to meet this expanding and diverse demand for spatial information utilization, it is necessary to develop technologies for linking and utilizing various attribute information such as public data. In this study, we aim to present a technology development strategy for linking and integrating attribute data and spatial information through a review of theories related to data linkage and integration, the current status of data on public data portals, and existing prior research. As a result, it was suggested that the data identifier of the attribute data to be linked should be used to develop linkage technology between spatial information and attribute data, and an attribute data linkage process that can be used when designing a prototype for technology development was presented.