• Food Science of Animal Resources
• /
• v.41 no.6
• /
• pp.936-949
• /
• 2021

This study discriminated fatty acid profile and flavor characteristics of Beijing You Chicken (BYC) as a precious local breed and Dwarf Beijing You Chicken (DBYC) eggs. Fatty acid profile and flavor characteristics were analyzed to identify differences between BYC and DBYC eggs. Four classification algorithms were used to build classification models. Arachidic acid, oleic acid (OA), eicosatrienoic acid, docosapentaenoic acid (DPA), hexadecenoic acid, monounsaturated fatty acids (MUFA), polyunsaturated fatty acids (PUFA), unsaturated fatty acids (UFA) and 35 volatile compounds had significant differences in fatty acids and volatile compounds by gas chromatography-mass spectrometry (GC-MS) (p<0.05). For fatty acid data, k-nearest neighbor (KNN) and support vector machine (SVM) got 91.7% classification accuracy. SPME-GC-MS data failed in classification models. For electronic nose data, classification accuracy of KNN, linear discriminant analysis (LDA), SVM and decision tree was all 100%. The overall results indicated that BYC and DBYC eggs could be discriminated based on electronic nose with suitable classification algorithms. This research compared the differentiation of the fatty acid profile and volatile compounds of various egg yolks. The results could be applied to evaluate egg nutrition and distinguish avian eggs.

• Nuclear Engineering and Technology
• /
• v.53 no.2
• /
• pp.446-454
• /
• 2021

Critical heat flux (CHF) has traditionally been evaluated using look-up tables or empirical correlations for nuclear power plants. However, under complex moving condition, it is necessary to reconsider the CHF characteristics since the conventional CHF prediction methods would no longer be applicable. In this paper, the additional forces caused by motions have been added to the annular film dryout (AFD) mechanistic model to investigate the effect of moving condition on CHF. Moreover, a theoretical model of the natural circulation loop with additional forces is established to reflect the natural circulation characteristics of the loop system. By coupling the system loop with the AFD mechanistic model, a CHF prediction program called NACOM for natural circulation loop under moving condition is developed. The effects of three operating conditions, namely stationary, inclination and rolling, on the CHF of the loop are then analyzed. It can be clearly seen that the moving condition has an adverse effect on the CHF in the natural circulation system. For the calculation parameters in this paper, the CHF can be reduced by 25% compared with the static value, which indicates that it is important to consider the effects of moving condition to retain adequate safety margin in subsequent thermal-hydraulic designs.

• Nuclear Engineering and Technology
• /
• v.53 no.4
• /
• pp.1236-1249
• /
• 2021

Although there are still controversial opinions and uncertainty on application of SiC_f/SiC composite cladding as next-generation cladding material for its great oxidation resistance in high temperature steam environment and other outstanding advantages, it cannot deny that SiC_f/SiC cladding is a potential accident tolerant fuel (ATF) cladding with high research priority and still in the engineering design stage for now. However, considering its disadvantages, such as low irradiated thermal conductivity, ductility that barely not exist, further evaluations of its in-pile behaviors are still necessary. Based on the self-developed code we recently updated, relevant thermohydraulic and mechanical models in FROBA-ATF were applied to simulate the cladding behaviors under normal and accident conditions in this paper. Even through steady-state performance analysis revealed that this kind of cladding material could greatly reduce the oxidation thickness, the thermal performance of UO₂-SiC was poor due to its low inpile thermal conductivity and creep rate. Besides, the risk of failure exists when reactor power decreased. With geometry optimization and dopant addition in pellets, the steady-state performance of UO₂-SiC was enhanced and the failure risk was reduced. The thermal and mechanical performance of the improved UO₂-SiC was further evaluated under Loss of coolant accident (LOCA) and Reactivity Initiated Accident (RIA) conditions. Transient results showed that the optimized ATF had better thermal performance, lower cladding hoop stress, and could provide more coping time under accident conditions.

• Journal of Microbiology and Biotechnology
• /
• v.29 no.3
• /
• pp.454-464
• /
• 2019

Salmonellosis is a highly contagious bacterial disease that threatens both human and poultry health. Tests that can detect Salmonella in the field are urgently required to facilitate disease control and for epidemiological investigations. Here, we combined loop-mediated isothermal amplification (LAMP) with a chromatographic lateral flow dipstick (LFD) to rapidly and accurately detect Salmonella. LAMP primers were designed to target the Salmonella invA gene. LAMP conditions were optimized by adjusting the ratio of inner to outer primers, $MgSO_4$ concentration, dNTP mix concentration, amplification temperature, and amplification time. We evaluated the specificity of our novel LAMP-LFD method using six Salmonella species and six related non-Salmonella strains. All six of the Salmonella strains, but none of the non-Salmonella strains, were amplified. LAMP-LFD was sensitive enough to detect concentrations of Salmonella enterica subsp. enterica serovar Pullorum genomic DNA as low as $89fg/{\mu}l$, which is 1,000 times more sensitive than conventional PCR. When artificially contaminated feed samples were analyzed, LAMP-LFD was also more sensitive than PCR. Finally, LAMP-LFD gave no false positives across 350 chicken anal swabs. Therefore, our novel LAMP-LFD assay was highly sensitive, specific, convenient, and fast, making it a valuable tool for the early diagnosis and monitoring of Salmonella infection in chickens.

• Nuclear Engineering and Technology
• /
• v.53 no.5
• /
• pp.1436-1445
• /
• 2021

To obtain the dynamic characteristics of reactor secondary circuit under transient conditions, the system analysis program was developed in this study, where dynamic models of secondary circuit were established. The heat transfer process and the mechanical energy transfer process are modularized. Models of main equipment were built, including main turbine, condenser, steam pipe and feedwater system. The established models were verified by design value. The simulation of the secondary circuit system was conducted based on the verified models. The system response and characteristics were investigated based on the parameter transients under emergency shutdown and overload. Various operating conditions like turbine emergency shutdown and overspeed, condenser high water level, ejector failures were studied. The secondary circuit system ensures sufficient design margin to withstand the pressure and flow fluctuations. The adjustment of exhaust valve group could maintain the system pressure within a safe range, at the expense of steam quality. The condenser could rapidly take out most heat to avoid overpressure.

• Journal of Microbiology and Biotechnology
• /
• v.31 no.8
• /
• pp.1115-1122
• /
• 2021

Rotavirus (RV), as the main cause of diarrhea in children under 5 years, contributes to various childhood diseases. Valeriana jatamansi Jones is a traditional Chinese herb and possesses antiviral effects. In this study we investigated the potential mechanisms of V. jatamansi Jones in RV-induced diarrhea. MTT assay was performed to evaluate cell proliferation and the diarrhea mice model was constructed using SA11 infection. Mice were administered V. jatamansi Jones and ribavirin. Diarrhea score was used to evaluate the treatment effect. The enzyme-linked immunosorbent assay was performed to detect the level of cytokines. Western blot and quantitative reverse transcription-PCR were used to determine protein and mRNA levels, respectively. Hematoxylin-eosin staining was applied to detect the pathological change of the small intestine. TdT-mediated dUTP nick-end labeling was conducted to determine the apoptosis rate. The results showed V. jatamansi Jones promoted MA104 proliferation. V. jatamansi Jones downregulated phosphatidylinositol 3-kinase (PI3K) and protein kinase B (AKT) in protein level, which was consistent with the immunohistochemistry results. Moreover, V. jatamansi Jones combined with ribavirin regulated interleukin-1β (IL-1β), interferon γ, IL-6, tumor necrosis factor α, and IL-10, and suppressed secretory immunoglobulin A secretion to remove viruses and inhibit dehydration. V. jatamansi Jones + ribavirin facilitated the apoptosis of small intestine cells. In conclusion, V. jatamansi Jones may inhibit RV-induced diarrhea through PI3K/AKT signaling pathway, and could therefore be a potential therapy for diarrhea.

• Nuclear Engineering and Technology
• /
• v.54 no.6
• /
• pp.1947-1953
• /
• 2022

As the plasma facing material in the nuclear fusion reactor, tungsten has to bear the irradiation impact of high energy particles. The surface quality of tungsten may affect its irradiation resistance, and even affect the service life of fusion reactor. In this paper, tungsten samples with different surface quality were polished by mechanical processing, subsequently conducted by D₂⁺ implantation and thermal desorption. D₂⁺ implantation was performed at room temperature (RT) with the irradiation dose of 1 × 10²¹ D₂⁺/m² by 5 keV D₂⁺ ions, and thermal desorption spectroscopy measurements were done from RT to 900 K. In addition, He irradiation was also performed by 50 eV He⁺ ions energy with the fluxes of 5.5 × 10²¹ m^-2s^-1 and 1.5 × 10²² m^-2s^-1, respectively. Results reveal that the hydrogen/helium irradiation behavior are both related to surface quality. Samples with high surface quality has superior D₂⁺ retention behavior with less D₂ retained after implantation. However, such samples are more likely to generate fuzzes on the surface after helium irradiation. Different morphologies (smooth, wavy, pyramids) after helium irradiation also demonstrates that the surface morphology is related to tungsten crystallographic orientation.

• Nuclear Engineering and Technology
• /
• v.54 no.2
• /
• pp.579-588
• /
• 2022

In order to meet the needs of domestic reactor severe accident analysis program, a MIDAC (Module Invessel Degraded severe accident Analysis Code) is developed and maintained by Xi'an Jiaotong University. As the accuracy of the calculation results of the analysis program is of great significance for the formulation of severe accident mitigation measures, the article select three experiments to evaluate the updated severe accident models of MIDAC. Among them, QUENCH-06 is the international standard No.45, QUENCH-16 is a test for the analysis of air oxidation, and FROMA is an out-of-pile fuel rod melting experiment recently carried out by Xi'an Jiaotong University. The heating and melting model with lumped parameter method and the steam oxidation model with Cathcart-Pawel and Volchek-Zvonarev correlations combination in MIDAC could better meet the needs of severe accident analysis. Although the influence of nitrogen still need to be further improved, the air oxidation model with NUREG still has the ability to provide guiding significance for engineering practice.

• Nuclear Engineering and Technology
• /
• v.54 no.10
• /
• pp.3909-3918
• /
• 2022

In the event of a severe accident, the reactor core may melt due to insufficient cooling. the high-temperature core melt will have a strong interaction (FCI) with the coolant, which may lead to steam explosion. Steam explosion would pose a serious threat to the safety of the reactors. Therefore, the study of steam explosion is of great significance to the assessment of severe accidents in nuclear reactors. This research focuses on the development of a two-dimensional steam explosion integrated analysis code called SEINA. Based on the semi-implicit Euler scheme, the three-phase field was considered in this code. Besides, the influence of evaporation drag of melt and the influence of solidified shell during the process of melt droplet fragmentation were also considered. The code was simulated and validated by FARO L-14 and KROTOS KS-2 experiments. The calculation results of SEINA code are in good agreement with the experimental results, and the results show that if the effects of evaporation drag and melt solidification shell are considered, the FCI process can be described more accurately. Therefore, it is proved that SEINA has the potential to be a powerful and effective tool for the analysis of steam explosions in nuclear reactors.

• Nuclear Engineering and Technology
• /
• v.53 no.3
• /
• pp.763-775
• /
• 2021

The thermal hydraulic performances of Steam Generator (SG) under both steady and transient operation conditions are of great importance for the safety and economy in nuclear power plants. In this paper, based on our self-developed SG thermal hydraulic analysis code STAF (Steam-generator Thermalhydraulic Analysis code based on Fluent), an improved new version STAF-CT (fully Coupling and Transient) is developed and introduced. Compared with original STAF, the new version code STAF-CT has two main functional improvements including "Transient" and "Fully Three Dimensional Coupling" features. In STAF-CT, a three dimensional energy transferring module is established which can achieve energy exchange computing function at the corresponding position between two sides of SG. The STAF-CT is validated against the international benchmark experiment data and the results show great agreement. Then the U-shaped SG in AP1000 nuclear power plant is modeled and simulated using STAF-CT. The results show that three dimensional flow fields in the primary side make significant effect on the energy source distribution between two sides. The development of code STAF-CT in this paper can provide an effective method for further SG high fidelity research in the nuclear reactor system.