• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.8
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• pp.5393-5399
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• 2014

To examine the effects of a mycosporine-like amino acids (MAAs) mixture from microalgae, Chlamydomonas sp, on the anti-wrinkle activities, the expression levels of genes that are associated with skin aging, including type I procollagen, elastin and involucrin, were analyzed. Asterina 330+palythine (A+P) and shinorine+palythine (S+P) mixtures were purified from Chlamydomonas sp using the following steps: 80% methanolic extraction, column purification, and HPLC analysis. As a result of the MTT assay, A+P and S+P did not induce cellular cytotoxicity with up to 0.1 mg/mL of both MAAs. In addition, the treatment of UV-exposed fibroblasts with A+P (0.05 mg/mL) and S+P (0.01 mg/mL) increased the levels of the PCOLCE mRNAs by 2.7 and 3.6 fold compared to the control group, respectively, The levels of elastin gene expression were elevated 5.59 and 3.1 fold in the A+P and S+P treated (0.01 mg/mL) cells, respectively. In particular, at a concentration of 0.01 mg /mL, the A+P and S+P expression levels of Involucrin mRNAs were increased 3.5 and 2.5 fold in the UV-exposed cells compared to the control, respectively. In conclusion, the MAAs derived from Chlamydomonas sp can be utilized as functional cosmetic materials with anti-wrinkle effects on the skin.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.30 no.2
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• pp.201-205
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• 2004

In these several years, as many people have been attracted by the functional cosmetics, there are a lot of study to enhance the stability of active ingredients for light, heat, oxygen, etc. in the academic and industrial field. Especially, catechin is well known as strong anti-oxidant, anti-inflammatory and reducing agent for oxidative stress but it is very unstable for light, heat, oxygen. etc. In this study, the stability and skin penetration of catechin are improved by 3-dimensional method. As I-dimension, porous silica is prepared using sol-gel method, and then catechin is adsorbed in pores of silica. As 2-dimension, solid lipid nanoparticles (SLN) are obtained using non-phospholipid vesicles. Finally 3-dimension is completion through lamellar phase self-organization that combines SLN catechin with skin lipid matrix. We used laser light scattering system, cyro-SEM, chromameter, HPLC and image analyzer to analyze our 3-dimentional systems. According to chromameter date, the color stability of 3-dimensional catechin is enhanced by 5-10 times compared with general liposome systems. We also confirmed through HPLC analysis that 3-dimensional catechin is more long lasting. The effect of skin penetration and wrinkle reduction are improved, too.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.36 no.2
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• pp.129-136
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• 2010

In order to evaluate anti-wrinkle activity of Carex humilis extract, free radical scavenging activity, elastase inhibitory activity and reduction of expression Matrix metalloproteinase-1 (MMP-1) mRNA and MMP-1 protein were investigated. The roots of Carex humilis were extracted with 95 % ethanol and successively partitioned with organic solvents with increasing polarity of the solvents. Each fraction of organic solvent were investigated by using free radical scavenging activity and elastase inhibitory activity test. Among them, EtOAc fraction showed antioxidant activity ($SC_{50}$=4.89 ${\mu}g/mL$) and elastase inhibitory activity ($IC_{50}$=23.5 ${\mu}g/mL$). EtOAc fraction was developed on silica gel by open-column chromatography and consecutively re-developed on C18 resin by prep-HPLC to give ${\alpha}$-viniferin as a major component, which was confirmed by spectrometric analysis. In the assay on expression of MMP-1 mRNA by RT-PCR and protein by western-blot, EtOAc layer (10 ~ 100 ${\mu}g/mL$) was reduced about 50 ~ 60 %, 50 ~ 65 % respectively and ${\alpha}$-viniferin (0.5 ~ 2 ${\mu}g/mL$) was inhibited about 60 ~ 75 %, 55 ~ 65 % respectively in human fibroblast. Therefore, our findings suggest that EtOAc layer of Carex humilis containing ${\alpha}$-viniferin can be useful as an active ingredient for cosmeceuticals of anti-wrinkle effects.

• Transactions of Materials Processing
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• v.32 no.6
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• pp.311-320
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• 2023

Recently, lightweight of automotive parts has been required to solve environmental problems caused by global warming. Accordingly, research and development are proceeded on manufacturing of parts using aluminum that can replace steel for lightweight of the automotive parts. In addition, high strength aluminum can be applied to body parts in order to meet both requirements of lightening and improving crash safety of vehicle. In this study, hot blow forming of roof side rail is employed to manufacturing of the automotive parts with high strength aluminum tube. In hot blow forming, longer forming times and excessive thinning can be occurred as compared with conventional manufacturing processes. So optimization of process conditions is required to prevent excessive thinning and to uniformize thickness distribution with fast forming time. Mechanical properties of high strength aluminum are obtained from tensile test at high temperature. These properties are used for finite element(FE) analysis to investigate the effect of strain rate on thinning and thickness distribution. Variation of thickness was firstly investigated from the result of FE analysis according to tube diameter, where the shapes at cross section of roof side rail are compared with allowable dimensional tolerance. Effective tube diameter is determined when fracture and wrinkle are not occurred during hot blow forming. Also FE analysis with various pressure-time profiles is performed to investigate the their effects on thinning and thickness distribution which is quantitatively verified with thinning factor. As a results, optimal process conditions can be determined for the manufacturing of roof side rail using high strength aluminum.

• Transactions of the Korean Society of Automotive Engineers
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• v.13 no.3
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• pp.64-73
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• 2005

This paper is concerned with the simulation-based parameter determination for the tool design in the stamping process of the complicated auto-body member. The CAE procedure of the stamping process is proposed so that troubles such as wrinkle, springback and excess metal be eliminated with changing parameters such as the blank size, the restraining force of the draw-bead and the embossing shape in the die face. The selected indicators of failure during forming are wrinkling. the amount of spring after unloading of the tool, the amount of excess metal developed .The proposed analysis scheme is applied to the tool and process parameter design for the front side member of a RV car. The simulation results show that the scheme can produce sound product from the viewpoint of thickness distribution, the contact condition between tools and the blank, the shape accuracy and so on.

• Transactions of Materials Processing
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• v.28 no.6
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• pp.328-335
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• 2019

Demand for ultra-thin materials is increasing due to their light-weight and versatile properties. In this work, the formability of the ultra-thin stainless steel sheets of various thicknesses in the incremental sheet forming (ISF) process is investigated. The effects of the thickness on formability were evaluated with forming experiments of the truncated cone shape with 10° intervals. As the thickness of the material decreased, the maximum forming angle decreased and wrinkles also occurred quickly. The maximum forming angles in the truncated cone shape without the wrinkles for the thickness of 0.05 mm, 0.08 mm, and 0.1mm were 30°, 40°, and 50°, respectively. Wrinkles occurred in a twisted shape along the moving direction of the tool. As the material thickness increased, the size of the wrinkles increased.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2005.06a
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• pp.1240-1243
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• 2005

The wrinkling in the flange and wall of a part is a predominant failure mode in stamping of sheet metal parts. In many cases this wrinkling may be eliminated by appropriate control of the blank holding force(BHF), but BHF affects the draw depth. Gotoh had studied the wrinkles under $20{\mu}$ in height. In general, the height of wrinkles could be limited under $200{\mu}$ practically. Therefore small BHF can be allowed so that the depth of drawing could be increased. This paper represents the variation of the wrinkles of flange in the part of cup drawing by using aluminium alloy A1050 and A5052. This simulation is used by the explicit finite elements code $PAM-STAMP^{TM}$. The computed results are compared with the experimental results to show the validity of the analysis.

• Journal of Advanced Marine Engineering and Technology
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• v.37 no.1
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• pp.66-71
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• 2013

Soft bending pipe is widely used for freezing equipment and shipbuilding. These pipe have some problems that they cause occasionally outside wrinkle, crack, wall thinning phenomena. However, vending machines which have been made to solve the problems completely, are not yet. In this study, to settle these problems, we proved the effectiveness of the self-made mandrel by results of simulation as the basic for the development of precision high-quality vending machine ; presents the solution method of the wall thinning phenomena by analyzing circular deformation and damage of bending pipe.

• Journal of Society of Preventive Korean Medicine
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• v.19 no.2
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• pp.83-101
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• 2015

Objective : In order to establish the scientific research methods to prove the effects of korean traditional medicine about ultraviolet induced skin damage, we investigated the research on ultraviolet induced skin damage in the korean traditional medicine field. Method : We searched the papers about ultraviolet induced skin damage published in the journals of korean traditional medicine. The searching end date was on June 29, 2015. Results : Thirty papers about ultraviolet induced skin damage were found. Twenty nine papers were experimental research, and one paper was clinical research. Sixty-three percent of papers were written the years after 2011. Fifty-seven percent of papers were published in the korean journal of oriental physiology & pathology and the journal of korean oriental medical ophthalmology & otolaryngology & dermatology. Main outcome measures of experimental research were anti-wrinkle effects, anti-oxidant capacity, anti-apoptosis effects, whitening effects and anti-inflammatory effects. Conclusion : We need more systematic research in order to prove the effects of korean traditional medicine, and use extensively.

• Transactions of Materials Processing
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• v.16 no.8
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• pp.621-628
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• 2007

Nowadays, Exhaust Gas Recirculation(EGR) Cooler is one of the most favorite systems for reducing the generation amount of $NO_x$ and other particle materials from vehicles burning diesel as fuel. Efficiency of the system is mainly dependent on its heat transfer efficiency and this ability is affected by net heat transferring area of the system. For that reason, several types of heat transfer tube such as dimple, wrinkle and spiral types that have large net area are used. However, it is difficult to manufacture the rectangular tube with dimpled type structure because it experiences too much strain around the rectangular tube surface during the forming process. For that reason, in this study, numerical simulation for forming process of non-symmetric dimple shape on a thin sheet metal was carried out. Furthermore, theoretical forming limit curves(forming limit diagram, forming limit stress diagram) were proposed as criteria of formability evaluation. From the results of finite element simulation in view of stress and strain distribution, it is found that the designed process has robustness and feasibility to safely manufacture the dimpled rectangular tube.