• 한국광학회지
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• 제34권2호
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• pp.45-52
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• 2023

본 연구에서는 다양한 파장의 저출력 light-emitting diode (LED)를 이용한 photobiomodulation (PBM)이 각막 상처 치유에 미치는 영향을 분석하였다. 각막상피세포에 623 nm에서 940 nm 범위의 파장의 LED를 조사한 결과, 유의미한 세포독성 영향을 미치지 않는 것을 확인하였다. PBM의 세포이동 촉진 효과를 세포 이동능 평가 시험을 통해 분석한 결과 623 nm 파장의 광조사에 의한 PBM이 세포이동을 크게 증가시키고 상처 치유를 촉진하는 것으로 나타났다. 또한, 세포이동 및 상처 치유와 관련된 유전자의 발현을 분석한 결과, 623 nm 파장의 광조사에 의한 PBM이 세포 증식과 세포 외 기질 분해를 촉진하는 것으로 알려진 FGF-1과 MMP2 유전자의 발현을 상향 조절한다는 사실을 발견했다. 이러한 연구 결과는 특정 파장, 특히 623 nm 파장의 저출력 빛을 이용한 PBM이 각막 손상 치료에 활용될 수 있는 가능성을 시사한다.

• 치위생과학회지
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• 제23권4호
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• pp.369-377
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• 2023

Background: In this study, we investigated the potential use of keratin for oral tissue regeneration. Keratin is well-known for its effectiveness in skin regeneration by promoting keratinization and enhancing the elasticity and activity of fibroblasts. Because of its structural stability, high storability, biocompatibility, and safety in humans, existing research has predominantly focused on its role in skin wound healing. Herein, we propose using keratin proteins as biocompatible materials for dental applications. Methods: To assess the suitability of alpha-keratin protein as a substrate for cell culture, keratin was extracted from human hair via PEGylation. Viabilities of primary human gingival fibroblasts (HGFs) and human oral keratinocytes (HOKs) were assessed. Fluorescence immunostaining and migration assays were conducted using a fluorescence microscope and confocal laser scanning microscope. Wound healing and migration assays were performed using automated software to analyze the experimental readout and gap closure rate. Results: We confirmed the extraction of alpha-keratin and formation of the PEG-g-keratin complex. Treatment of HGFs with keratin protein at a concentration of 5 mg/ml promoted proliferation and maintained cell viability in the test group compared to the control group. HOKs treated with 5 mg/ml keratin exhibited a slight decrease in cell proliferation and activity after 48 hours compared to the untreated group, followed by an increase after 72 hours. Wound healing and migration assays revealed rapid closure of the area covered by HOKs over time following keratin treatment. Additionally, HOKs exhibited changes in cell morphology and increased the expression of the mesenchymal marker vimentin. Conclusion: Our study demonstrated the potential of hair keratin for soft tissue regeneration, with potential future applications in clinical settings for wound healing.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2010년도 춘계학술대회 논문집
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• pp.1409-1410
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• 2010

• Animal cells and systems
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• 제15권2호
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• pp.139-146
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• 2011

Secretory leukocyte protease inhibitor (SLPI) plays an important role in promoting the invasion and metastasis of a range of cancer cells. However, there are no reports of the expression and function of SLPI in oral carcinoma cells. In this study, the oral carcinoma cell line KB was used to determine whether SLPI affects the proliferation, migration and invasion of oral carcinoma cells. RT-PCR and Western blotting revealed high levels of endogenous SLPI expression in KB cells as well as a strong increase in SLPI secretion after wounding compared to immortalized normal oral keratinocytes (INOK). The wound healing assay revealed more migration of KB cells than INOK cells, and the SLPI treatment increased the migration of KB cells. KB cell proliferation was increased significantly by the SLPI protein but decreased by SLPI-siRNA. SLPI strongly increased the migration and invasion of KB cells. On the other hand, SLPI-siRNA decreased the migration and invasion of KB cells. This suggests that SLPI plays an important role in the metastasis of oral carcinoma cells.

• Restorative Dentistry and Endodontics
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• 제45권1호
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• pp.2.1-2.7
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• 2020

Objectives: This study aimed to evaluate the cell viability and migration of Endosequence Bioceramic Root Canal Sealer (BC Sealer) compared to MTA Fillapex and AH Plus. Materials and Methods: BC Sealer, MTA Fillapex, and AH Plus were placed in contact with culture medium to obtain sealers extracts in dilution 1:1, 1:2 and 1:4. 3T3 cells were plated and exposed to the extracts. Cell viability and migration were assessed by 3-(4,5-dimethylthiazoyl)-2,5-diphenyl-tetrazolium bromide (MTT) and Scratch assay, respectively. Data were analyzed by Kruskal-Wallis and Dunn's test (p < 0.05). Results: The MTT assay revealed greater cytotoxicity for AH Plus and MTA Fillapex at 1:1 dilution when compared to control (p < 0.05). At 1:2 and 1:4 dilutions, all sealers were similar to control (p > 0.05) and MTA Fillapex was more cytotoxic than BC Sealer (p < 0.05). Scratch assay demonstrated the continuous closure of the wound according to time. At 30 hours, the control group presented closure of the wound (p < 0.05). At 36 hours, only BC Sealer presented the closure when compared to AH Plus and MTA Fillapex (p < 0.05). At 42 hours, AH Plus and MTA Fillapex showed a wound healing (p > 0.05). Conclusions: All tested sealers demonstrated cell viability highlighting BC Sealer, which showed increased cell migration capacity suggesting that this sealer may achieve better tissue repair when compared to other tested sealers.

• 한방안이비인후피부과학회지
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• 제14권2호
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• pp.89-111
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• 2001

In order to investigate the effects of GamiTakliSodocyum(GTS) on wound healing, migration of epidermis, formation of granulation tissue and number of capillary within the granulation tissue were measured in diabetic mice by local application and NZW rabbits by local application and prescription of medicine in vivo, and proliferation of human epidermal keratinocytes and human dermal fibroblasts and composition of extracellular matrix were measured in vitro. The results were summerized as follows. 1. $2\%,\;10\%$ GTS remarkably increased migration of epidermis in diabetic mice by local application. 2. $2\%,\;10\%$ GTS remarkably increased formation of granulation tissue, number of neovascularization within the granulation tissue in diabetic mice by local application. 3. 5\%,\;10\%$ GTS remarkably increased migration of epidermis in NZW rabbits by local application. 4. $5\%,\;10\%$ GTS remarkably increased fonnation of granulation tissue, number of neovascularization within the granulation tissue in NZW rabbits by local application. 5. $5\%,\;10\%$ GTS increased migration of epidennis in NZW rabbits by prescription of medicine. 6. $5\%,\;10\%$ GTS increased formation of granulation tissue, number of neovascularization within the granulation tissue in NZW rabbits by prescription of medicine. 7. GTS didn't show effect on the proliferation of human epidermal keratinocytes. 8. GTS increased the proliferation of cultured human dermal fibroblasts. 9. GTS increased the expression of procoliagen ${\alpha}1(I) mRNA in cultured human dermal fibroblasts. 10. GTS increased the expression of fibronectin mRNA in cultured human dennal fibroblasts according to dosage of GTS using northern blot hybridization but didn't increase, using RT-PCR. From the above results, it is conclude that GTS might use on wound healing.

• 대한의생명과학회지
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• 제7권1호
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• pp.17-25
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• 2001

This study was performed to assess the effects of exogenous collagen gel for the re-epithelialization of partial thickness skin wound healing in rabbits. Adult male rabbits (New Zealand White Rabbit) 1.5~2 kg, were used for experimental animals. Skin wounds (1.5$\times$2 cm length) were created bilaterally on the flank of 10 rabbits and then treated a periods of 9 days. Wounds on the experimental site were treated with exogenous collagen gel as well as fabric material gauze dressing. Control site wounds were covered with fabric material gauze dressing alone. Histological findings indicated that the epithelial migration of the experimental site of rabbits was far more rapid than that in the other control wound sites. Moreover, exogenous collagen gel provided a moist environment to keep wound clean, and facilitate keratinocyte proliferation. The wound dressed with exogenous collagen gel demonstrated a significant increase in the healing rate and re-epithelialization.

• 한국임상수의학회지
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• 제11권1호
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• pp.427-436
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• 1994

To investigate the effect of yeast glucan on wound healing in rabbits, histopathological findings and wound contraction after topical application of glucan on the open wound were observed. Formalin fixation and embedding of tissues at wound were prepared for histopathological findings at the other day after wounding. Wound size was measured for contraction. Macrophages appeared in the deeper site under the scab at day 1 after wounding in the glucan treated group, but not in the control. A number of macrophages, fibroblasts and a small number of neutrophils were observed at day 3 after wounding In the glucan treated group as compared with the control. Histopathological findings at day 5 after wounding in control were similar with those at day 3 in the glucan treated group. Glucan like granules were scattered in the cytoplasm of macrophages at day 5 in the glucan treated group. A great portion of wound reepithelialized at day 5 in the glucan treated group but a small portion of wound in the control. The Present study indicated that the treatment of 5,000$\mu\textrm{g}$/$m\ell$ of glucan has an effect on wound heal. ins in respect to migration of macrophage into the wound site, granulation tissue formation and epithelialization.

• Journal of Ginseng Research
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• 제47권5호
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• pp.654-661
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• 2023

Background: Ginseng has been used as a traditional medicine and functional cosmetic ingredients for many years. Recent studies have focused on the potential biological effects of the ginseng berry and its ingredients. (+)-Syringaresinol (SYR) is enriched in ginseng berry and its beneficial effects on the skin have been recently reported. However, little is known about the its effects on the wound healing process of skin. Methods: Here, we evaluated the skin wound healing effect of (+)-SYR using the human fibroblast Hs68 cell and ex vivo pig and human skin tissue model. Scratch wound test and hydrogen peroxide (HPO) induce chemical wound model were employed. Results: (+)-SYR promoted the migration and proliferation of Hs68 cells without significant cytotoxicity at the tested concentrations. Especially, in ex vivo pig and human skin tissue, HPO-induced chemical wound was recovered almost completely by (+)-SYR. In line with the finding in Hs68, the protein expression levels of TGF-β and PCNA, a proliferation marker were increased, demonstrating the beneficial effects of (+)-SYR on skin wound repair. Conclusion: Collectively, we demonstrated that (+)-SYR from ginseng berry, can enhance the wound healing effect by accelerating cell proliferation and skin regeneration, suggesting the potential utility of (+)-SYR for skin wound repair.

• Journal of Microbiology and Biotechnology
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• 제26권6호
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• pp.1140-1147
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• 2016

The plasma and serum of Crocodylus siamensis have previously been reported to exhibit potent antimicrobial, antioxidant, and anti-inflammatory activities. During wound healing, these biological properties play a crucial role for supporting the formation of new tissue around the injured skin in the recovery process. Thus, this study aimed to evaluate the wound healing properties of C. siamensis plasma and serum. The collected data demonstrate that crocodile plasma and serum were able to activate in vitro proliferation and migration of HaCaT, a human keratinocyte cell line, which represents an essential phase in the wound healing process. With respect to investigating cell migration, a scratch wound experiment was performed which revealed the ability of plasma and serum to decrease the gap of wounds in a dose-dependent manner. Consistent with the in vitro results, remarkably enhanced wound repair was also observed in a mouse excisional skin wound model after treatment with plasma or serum. The effects of C. siamensis plasma and serum on wound healing were further elucidated by treating wound infections by Staphylococcus aureus ATCC 25923 on mice skin coupled with a histological method. The results indicate that crocodile plasma and serum promote the prevention of wound infection and boost the re-epithelialization necessary for the formation of new skin. Therefore, this work represents the first study to demonstrate the efficiency of C. siamensis plasma and serum with respect to their wound healing properties and strongly supports the utilization of C. siamensis plasma and serum as therapeutic products for injured skin treatment.