• Parasites, Hosts and Diseases
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• v.57 no.1
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• pp.49-53
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• 2019

Contaminated liver fluke egg in the environment has led to the high prevalence of human opisthorchiasis associated with cholangiocarcinoma in Southeast Asia. To find the effective lessening methods of Opisthorchis viverrini eggs in the contaminated environment, we investigated the temperature conditions for killing of these trematode eggs in vitro. Numerous O. viverrini eggs were obtained in the proximal part of uteri of adult worms from experimental hamsters. Mature eggs with miracidium were allocated by experimental groups (2 control: positive and negative and 4 treatment: 50, 60, 70, and $80^{\circ}C$) with 0.85% saline, and treated by the experimental plan. Eggs in each experimental groups were observed under the confocal microscope after stain with Propidium Iodide (PI) to evaluate the effect of temperatures. Eggs in 70 and $80^{\circ}C$ groups were all killed after over 10 min heated. Majority of eggs in $60^{\circ}C$ (10, 15, and 30 min heated), 70 and $80^{\circ}C$ (5 min heated) groups were inactivated. However in $50^{\circ}C$ group, below half of eggs were to be killed in all time lapse (10, 15 and 30 min). In order to prevent O. viverrini infection and cholangiocarcinoma, direct treatment of sewage by heating at 70 or $80^{\circ}C$ at least 10 min is essential. Therefore, treatment of O. viverrini eggs at a high temperature is a potential method for controlling egg contamination in sewage.

• Molecules and Cells
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• v.41 no.12
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• pp.1052-1060
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• 2018

Triclosan (TCS) is a phenolic antimicrobial chemical used in consumer products and medical devices. Evidence from in vitro and in vivo animal studies has linked TCS to numerous health problems, including allergic, cardiovascular, and neurodegenerative disease. Using Caenorhabditis elegans as a model system, we here show that short-term TCS treatment ($LC_{50}$: ~0.2 mM) significantly induced mortality in a dose-dependent manner. Notably, TCS-induced mortality was dramatically suppressed by co-treatment with non-ionic surfactants (NISs: e.g., Tween 20, Tween 80, NP-40, and Triton X-100), but not with anionic surfactants (e.g., sodium dodecyl sulfate). To identify the range of compounds susceptible to NIS inhibition, other structurally related chemical compounds were also examined. Of the compounds tested, only the toxicity of phenolic compounds (bisphenol A and benzyl 4-hydroxybenzoic acid) was significantly abrogated by NISs. Mechanistic analyses using TCS revealed that NISs appear to interfere with TCS-mediated mortality by micellar solubilization. Once internalized, the TCS-micelle complex is inefficiently exported in worms lacking PMP-3 (encoding an ATP-binding cassette (ABC) transporter) transmembrane protein, resulting in overt toxicity. Since many EDCs and surfactants are extensively used in commercial products, findings from this study provide valuable insights to devise safer pharmaceutical and nutritional preparations.

• Genomics & Informatics
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• v.17 no.1
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• pp.7.1-7.10
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• 2019

Cysticercosis, a parasitic disease caused by Taenia solium metacestode (TsM), has a major global public health impact in terms of disability-adjusted life years. The parasite preferentially infects subcutaneous tissue, but may invade the central nervous system, resulting in neurocysticercosis (NC). NC is an important neglected tropical disease and an emerging disease in industrialized countries due to immigration from endemic areas. The prevalence of taeniasis in Korea declined from 0.3%-12.7% during the 1970s to below 0.02% since the 2000s. A survey conducted from 1993 to 2006 revealed that the percentage of tested samples with high levels of specific anti-TsM antibody declined from 8.3% to 2.2%, suggesting the continuing occurrence of NC in Korea. Modern imaging modalities have substantially improved the diagnostic accuracy of NC, and recent advances in the molecular biochemical characterization of the TsM cyst fluid proteome also significantly strengthened NC serodiagnosis. Two glycoproteins of 150 and 120 kDa that induce strong antibody responses against sera from patients with active-stage NC have been elucidated. The 150 kDa protein showed hydrophobic-ligand binding activities and might be critically involved in the acquisition of host-derived lipid molecules. Fasciclin and endophilin B1, both of which play roles in the homeostatic functions of TsM, showed fairly high antibody responses against calcified NC cases. NC is now controllable and manageable. Further studies should focus on controlling late-onset intractable seizures and serological diagnosis of NC patients infected with few worms. This article briefly overviews diagnostic approaches and discusses current issues relating to NC serodiagnosis.

• Animal Bioscience
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• v.34 no.2
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• pp.185-191
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• 2021

Objective: The objective of this study was to estimate the genetic parameters for worm resistance (WR) and associated characteristics, using the linear-threshold animal model via Bayesian inference in single- and multiple-trait analyses. Methods: Data were collected from a herd of Santa Inês breed sheep. All information was collected with animals submitted to natural contamination conditions. All data (number of eggs per gram of feces [FEC], Famacha score [FS], body condition score [BCS], and hematocrit [HCT]) were collected on the same day. The animals were weighed individually on the day after collection (after 12-h fasting). The WR trait was defined by the multivariate cluster analysis, using the FEC, HCT, BCS, and FS of material collected from naturally infected sheep of the Santa Inês breed. The variance components and genetic parameters for the WR, FEC, HCT, BCS, and FS traits were estimated using the Bayesian inference under the linear and threshold animal model. Results: A low magnitude was obtained for repeatability of worm-related traits. The mean values estimated for heritability were of low-to-high (0.05 to 0.88) magnitude. The FEC, HCT, BCS, FS, and body weight traits showed higher heritability (although low magnitude) in the multiple-trait model due to increased information about traits. All WR characters showed a significant genetic correlation, and heritability estimates ranged from low (0.44; single-trait model) to high (0.88; multiple-trait model). Conclusion: Therefore, we suggest that FS be included as a criterion of ovine genetic selection for endoparasite resistance using the trait defined by multivariate cluster analysis, as it will provide greater genetic gains when compared to any single trait. In addition, its measurement is easy and inexpensive, exhibiting greater heritability and repeatability and a high genetic correlation with the trait of resistance to worms.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.14 no.4
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• pp.97-110
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• 2004

As modem web services become enormously complex, web attacks has become frequent and serious. Existing security solutions such as firewalls or signature-based intrusion detection systems are generally inadequate in securing web services, and analysis of raw web log data is simply impractical for most organizations. Visual display of "interpreted" web logs, with emphasis on anomalous web requests, is essential for an organization to efficiently track web usage patterns and detect possible web attacks. In this paper, we discuss various issues related to effective real-time visualization of web usage patterns and anomalies. We implemented a software tool named SAD (session anomaly detection) Viewer to satisfy such need and conducted an empirical study in which anomalous web traffics such as Misuse attacks, DoS attacks, Code-Red worms and Whisker scans were injected. Our study confirms that SAD Viewer is useful in assisting web security engineers to monitor web usage patterns in general and anomalous web sessions in particular.articular.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.17 no.3
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• pp.43-53
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• 2007

Internet becomes more and more popular, and most companies and institutes use web services for e-business and many other purposes. With the explosion of Internet, the occurrence of cyber terrorism has grown very rapidly. Simulation is one of the most widely used method to study internet worms. But, it is quite challenging to simulate very large-scale worm attacks because of various reasons. In this paper, we propose a hybrid modeling method for RCS(Random Constant Spreading) worm simulation. The proposed hybrid model simulates worm attacks by synchronizing modeling network and packet network. So, this model will be both detailed enough to generate realistic packet traffic, and efficient enough to model a worm spreading through the Internet. Moreover, our model have the capability of dynamic updates of the modeling parameters. Finally, we simulate the hybrid model with the CodeRed worm to show validity of our proposed model for RCS worm simulation.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.18 no.1
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• pp.89-102
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• 2008

Internet becomes more and more popular, and most companies and institutes use web services for e-business and many other purposes. With the explosion of Internet, the attack of internet worm has grown. Simulation is one of the most widely used method to study internet worms. But, it is quite challenging to simulate very large-scale worm attacks because of various reasons. By this reason, we often use the modeling network simulation technique. But, it also has problem that it difficult to apply each worm attacks to simulation. In this paper, we propose worm attack representation and mapping methods for apply worm attack to simulation. The proposed method assist to achieve the simulation efficiency. And we can express each worm attacks more detail. Consequently, the simulation of worm attacks has the time-efficiency and the minuteness.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.16 no.1
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• pp.55-63
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• 2006

Recently, there is much abnormal traffic driven by several worms, such as Nimda, Code Red, SQL Stammer, and so on, making badly severe damage to networks. Meanwhile, diverse prevention schemes for defeating abnormal traffic have been studied in the academic and commercial worlds. In this paper, we present the structure of a stepwise intrusion prevention system that is designed with the feature of putting limitation on the network bandwidth of each network traffic and dropping abnormal traffic, and then compare the proposed scheme with a pre-existing scheme, which is a True/False based an anomaly prevention scheme for several worm-patterns. There are two criteria for comparison of the schemes, which are Normal Traffic Rate (NTR) and False Positive Rate (FPR). Assuming that the abnormal traffic rate of a specific network is $\beta$ during a predefined time window, it is known that the average NTR of our stepwise intrusion prevention scheme increases by the factor of (1+$\beta$)/2 than that of True/False based anomaly prevention scheme and the average FPR of our scheme decrease by the factor of (1+$\beta$)/2.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.19 no.4
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• pp.29-39
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• 2009

In order to respond against worms which are malicious programs automatically spreading across communication networks, worm detection approach by generating signatures resulting from analyzing worm-related packets is being mostly used. However, to avoid such signature-based detection techniques, usage of exploits employing mutated polymorphic types are becoming more prevalent. In this paper, we propose a novel static analysis approach for detecting the decryption routine of polymorphic exploit code, Our approach detects a code routine for performing the decryption of the encrypted original code which are contained with the polymorphic exploit code within the network flows. The experiment results show that our approach can detect polymorphic exploit codes in which the static analysis resistant techniques are used. It is also revealed that our approach is more efficient than the emulation-based approach in the processing performance.

• Parasites, Hosts and Diseases
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• v.60 no.5
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• pp.361-365
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• 2022

We report a species of diplostomid fluke recovered from 3 carcasses of wild Korean raccoon dog, Nyctereutes procyonoides koreensis, in Korea. A total of 107 diplostomid flukes were recovered from the small intestines of Korean raccoon dogs, which were obtained from the Gangwon Wildlife Medical Rescue Center. Worms fixed with 10% neutral formalin were subjected to microscopic observation and those fixed in 70% ethanol were used for molecular genomic analysis. The worm was divided into 2 separate parts, forebody and hindbody, with a total length of 3,020-4,090 (3,855) ㎛ and a width of 1,210-1,770 (1,562) ㎛. The boat-shaped forebody has a pair of characteristic tentacular appendage, 2 suckers, holdfast organ, and vitelline follicles. The oval to cylindrical hindbody has reproductive organs. The ovary was round or elliptical and located in the anterior of the testes. Two large testes were slightly segmented and tandemly arranged, occupying almost half of hindbody. The short uterus contained a relatively small number of unembryonated eggs sized 130-140×85-96 ㎛. The partial sequence of 18S rRNA of this fluke was consistent with Alaria alata. Based on the morphological and molecular characteristics, the diplostomid flukes recovered from the small intestine of Korean raccoon dogs were identified as A. alata (Digenea: Diplostomidae).