• Korean Journal of Food Science and Technology
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• v.36 no.3
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• pp.440-447
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• 2004

Characteristics of human ${\beta}-carotene$ 15,15'-dioxygenase isolated by recombinant DNA technology was elucidated. Optimal pH and temperature were 9.0 and $40^{\circ}C$, respectively. Enzyme activity was temperature-sensitive. Enzyme was stable at pH 6.0-9.0 for 24 hr and under $5^{\circ}C$. Half-life of enzyme at $35^{\circ}C$ was 40 min. Crude preparations of enzyme were inhibited by ferrous ion-chelating agent and sulfhydryl-binding agent. GSH offsets inhibitory effect of PCMB. With increasing substrate concentrations, enzyme activity gave typical Michaelis-Menten curve, Based on Hanes-Woolf plot of data, $K_{m}\;and\;V_{max$ were $3.39{\times}10^{6}\;M\;and\;1.2\;pmol/mg$ protein/min, respectively.

• Microbiology and Biotechnology Letters
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• v.23 no.6
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• pp.710-716
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• 1995

$\beta $-1, 4-D-arabinogalactanase isolated from alkalophilic Bacillus sp. HJ-12, approximate Mw 42 kDa, was generally stable in the range of pH 6-10 and below 50$\circ$C and its highest activity was observed at 60$\circ$C with pH 7-9. The isolated $\beta $-1, 4-D-arabinogalactanase specifically hydrolyzed $\beta $-1, 4-galactosyl linkage that is the major structure of soybean arabinogalactan (SAG) but not $\beta $-1, 3-galactosyl linkage of the other polysaccharides. K. was estimated as 0.67 mg/ml by the method of Hanes-Woolf plot. No metals and chemical reagents inhibited the enzyme activity but urea did. The active site of this enzyme assumed to be tryptophan residue. The hydrolysis products from SAG, assayed by gel chromatography, TLC and HPLC, were predominantly galactotetraose (Gal$_{4}$) and triose (Gal$_{3}$) with a small portion. $\beta $-1, 4-D-arabinogalactanase hydrolyzed ONPG as well as SAG, and the degree of hydrolysis of SAG was 15% which is lower than that by the other $\beta $-1, 4-galactanases from different sources. SAG treated with this enzyme resulted in the reduction of specific viscosity up to 70%.

• Acta Via Serica
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• v.4 no.1
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• pp.71-86
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• 2019

Transnational exchange has been an inseparable part of both the ancient and modern Silk Road. This paper shows how Rumi (1207-1273), a famous Persian Sufi poet, travels along the Silk Road in the $21^{st}$ century. With the birth of a Rumi phenomenon in the West, Silk Road artists have rediscovered and adapted him for different purposes. Elif Shafak, the Turkish-British novelist and women's rights activist, espouses feminist beliefs in her bestseller, The Forty Rules of Love (2010). Benefiting from the views of feminist theorists like Woolf, de Beauvoir and Friedan, this paper reveals how Shafak appropriates Rumi for her feminist purposes. Forty Rules of Love's protagonist, Ella Rubinstein is analyzed, compared and contrasted with her former literary counterparts Pinhan and Zeliha, heroines of Shafak's previous novels. By adapting Rumi's definition of equality, Shafak shows how egalitarianism must pervade the relationship between women and men. The adaptation of Rumi's ideas regarding the equality of sexes finds a different dimension when Shafak reveals that all humanity possesses femininity and masculinity at the same time. By means of ideas prevalent in the ancient Silk Road, the five classical elements theory, and the yin and yang principle, Shafak portrays unity within contradictions. It is concluded that although individuals might belong to different typologies of the five symbolic elements of nature, they can at the same time complement one another's inharmonious personalities peacefully. The process of integration of female and male sexes can be expedited by opening up one's heart to a universal love.

• Cross-Cultural Studies
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• v.53
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• pp.219-249
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• 2018

This essay attempts a historical examination of educational function of the novel. It pays attention to the eighteenth century sentimentalism, and its historical vicissitudes up to early twenties century. The eighteenth century is the period in which debates on the nature of emotion and its moral and aesthetic role have passionately taken place and the modern paradigm of thought on affect has been formed. This is why "affect revival phenomenon" in the late twenties century goes back to this period. This essay finds in Adam Smith the most sophisticated arguments on sympathy in their relation to the development of the novel; it examines the relationship of Smith's argument with modern novel in the tradition of sentimentalism, and its revision in modernist novel. Through this examination, it discusses how cognitive and non-cognitive approaches, the two representative positions in contemporary thinking on emotion/affect, have revised and transformed the eighteenth century sentimentalism.

• Journal of English Language & Literature
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• v.56 no.6
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• pp.1067-1090
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• 2010

The epistolary novel, as developed and refined by Samuel Richardson (1689-1761), is concerned with distinctly private experience and the morality of individuals-Richardson's heroine writers. In contrast to nineteenth-century novels, which explore their subjects through the overview of a narrator with a singular moral outlook, the epistolary narrative allows Richardson to examine the various different ways in which individuals/heroines interpret, mold, and respond to their experiences in writing. In this paper, I argue that the authorial voice of Richardson does not control the narrative but rather is present in the prefaces, character sketches, notes and occasional interjections between letters. Although there is little doubt as to whether Richardson intended to make a particular moral point or attempted to control the effect of his novels on his readers, the heroines and their letters dominate the novels so that they put the authorial suggestions in a different light, reducing the author's to one voice among several. Thus, Pamela's letters are exemplary for the vigor and intelligence with which they appear to be written, rather than for the imposed morality of their ghost writer-Richardson. Although Clarissa is of a different social class from Pamela, both heroines are united in their oppression as victims of a patriarchal society. In Clarissa's letters, the heroine's situation and experience are seen through her own writing in dialogue with that of her confidante Anna Howe, and in contrast to the writing of her oppressors. Clarissa, then, becomes a struggle between different discourses in which their genesis and effect, and the societies and individuals from which they come are implicitly suggested in Richardson's text. While Richardson may or may not be guilty of taking the writing of women and using it for his own ends, his epistolary novels represent a deliberate and bold attempt to shape the novel in a way conducive to his heroines and to women writers.