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Leukocyte-Endothelial Cell Adhesion Induced by Ischemia and Reperfusion Observed with in vivo Videomicroscopy (허혈-재관류에 의해 유도된 백혈구-혈관내피세포 유착에 대한 Videomicroscopy 영상소견)

  • Lee, Young Bae;Kang, Han Sug;Park, Shin Byung
    • Journal of Korean Neurosurgical Society
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    • v.29 no.10
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    • pp.1289-1295
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    • 2000
  • Purpose : Recent evidence suggests a possible role for leukocytes in brain injury following ischemia and reperfusion. This study examined the temporal profile of ischemic tissue damage and leukocyte response after transient middle cerebral artery occlusion(MCAO) with reperfusion in the mouse. Methods : Focal cerebral ischemia was made by temporary occluding of the stem of the proximal MCA. Two groups of the mouse were investigated : (1) sham operation(n=10), and (2)those having the arterial occlusion released after 90 minute(n=20). By 4 hours(n=10) and 24 hours(n=10) after the onset of ischemia-reperfusion, fluorescein videoimages were under-taken in the pial venules of the mouse using a closed cranial window technique. Rhodamine 6G was administered as a $80-100{\mu}l/min$ i.v. loading dose and a $30-40{\mu}l/min$ i.v. maintenance dose in saline to selectively label circulating leukocytes. Neuropathologic evaluation for brain injury was accomplished using the histochemical stain 2,3,5-triphen-yltetrazolium chloride(TTC) and hematoxylin and eosin(H & E) stain. Results : The mean number of adherent leukocytes to cerebral venules in the 90 minutes MCAO and 24 hours reperfusion group were $306{\pm}24$ compared with $72{\pm}8$ in the sham operation group. In the TTC staining method, the cortical infarct affecting 34.8% of hemispheric volume were created in all of animals (n=10) undergoing 90 minute MCAO with 24 hours reperfusion, but the infarcted area were not found in the other(sham operation and 90 minute MCAO with 4 hours reperfusion)groups. In the H & E stain, the brain tissue following 90 minute MCAO with 4 hours reperfusion revealed only a pyknosis of the nuclei with shrunken cytoplasm, but infiltrated leukocytes were not observed. After 24 hours of reperfusion, a many leukocytes were infiltrated within parenchyma and blood vessles. Conclusions : These findings demonstrate the feasiblity of continous in vivo monitoring of leukocyte adherence in cerebral venules and suggest that reperfusion induced leukocyte adherence to venular endothelium may contribute to tissue injury following focal cerebral ischemia.

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Expression of Cytokines in Radiation Injured Brain at Acute Phase

  • Lee, Jang-Bo;Kim, Min-Ho;Chung, Yong-Gu;Park, Jung-Yul
    • Journal of Korean Neurosurgical Society
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    • v.42 no.3
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    • pp.200-204
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    • 2007
  • Objective : Radiation therapy is an important treatment for brain tumor. However, serious complications such as radiation necrosis can occur and it may be secondary to the expression of acute phase genes, like cytokines. In particular, inflammatory cytokines (IL-$1{\beta}$, TNF-${\alpha}$) and other immunomodulatory cytokines (TNF-${\alpha}$, TGF-${\beta}1$) might be changed after irradiation (high single dose irradiation). Although it has been reported that IL-1 level is remarkably elevated within 8 week after the irradiation to the rat brain. the change of cytokines levels at acute phase (within 24 hours) has not been reported. In the present study, we examined TNF-${\alpha}$, TGF-${\beta}1$, and IL-$1{\beta}$ levels in acute phase to clarify the early effect of cytokines on the radiation-induced brain damage. Methods : Fifty Sprague-Dawley rats were used and these were divided into irradiation group and control group. After a burr-hole trephination on the right parietal area using a drill, a single 10Gy was irradiated at the trephined site. Their forebrains were extirpated at 30 min, 2 hr, 8 hr, 12 hr and 24 hr, respectively and examined for the expression of TNF-${\alpha}$, TGF-${\beta}1$, and IL-$1{\beta}$. Results : The expression of TNF-${\alpha}$ and TGF-${\beta}1$ were decreased until 12 hr after irradiation but elevated thereafter. The expression of IL-1 was peak at 8 hr and then decreased until 12 hr but elevated after this time window. The present study indicated that expression of cytokines (TNF-${\alpha}$, TGF-${\beta}1$ and IL-$1{\beta}$) were increased at 24 hr after the irradiation to the rat brain. IL-$1{\beta}$ level, on the other hand. reached peak at 8 hr after radiation injury. Conclusion : These findings indicate that IL-1, among various cytokines, may have a more important role in the inflammatory reaction by radiation injury at acute phase and provide some clues for better understanding of the pathogenesis of radiation injury.

Efficiency analysis of Oriental hospitals according to characteristics (한방병원 특성별 경영효율성 분석)

  • Kim, Young-Sik;Lee, Woo-Chun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.5
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    • pp.59-67
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    • 2017
  • This study analyzes the efficiency of oriental hospitals using DEA(Data Envelopment Analysis). The input variables include the numbers of doctors, nurses, medical technicians, and beds. The output variable iscomprised of the sales account. The analysis tools used are EnPas and IBM SPSS Statistics 19. As a result of efficiency analysis, the private hospitals(establishment), less than 10 years in operation(operating period), containing less than 50 beds (number of the beds), located in the metropolitan area(location) showed high efficiency in the BCC(Banker, Charnes & Cooper) model, but indicated relatively low efficiency in CCR(Charnes, Cooper & Rhodes) model. This contradictory result is caused by inefficiencies in hospital size. The logistic regression analysis conducted to analyze the variables that affect the efficiency of oriental hospitals found that the efficiency decreased by 0.955 with each increase of 1 bed in the hospital.

A Study on the Optimization of the SiNx:H Film for Crystalline Silicon Sloar Cells (결정질 실리콘 태양전지용 SiNx:H 박막 특성의 최적화 연구)

  • Lee, Kyung-Dong;Kim, Young-Do;Dahiwale, Shailendra S.;Boo, Hyun-Pil;Park, Sung-Eun;Tark, Sung-Ju;Kim, Dong-Hwan
    • Journal of the Korean Vacuum Society
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    • v.21 no.1
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    • pp.29-35
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    • 2012
  • The Hydrogenated silicon nitride (SiNx:H) using plasma enhanced chemical vapor deposition is widely used in photovoltaic industry as an antireflection coating and passivation layer. In the high temperature firing process, the $SiN_x:H$ film should not change the properties for its use as high quality surface layer in crystalline silicon solar cells. Initially PECVD-$SiN_x:H$ film trends were investigated by varying the deposition parameters (temperature, electrode gap, RF power, gas flow rate etc.) to optimize the process parameter conditions. Then by varying gas ratios ($NH_3/SiH_4$), the hydrogenated silicon nitride films were analyzed for its optical, electrical, chemical and surface passivation properties. The $SiN_x:H$ films of refractive indices 1.90~2.20 were obtained. The film deposited with the gas ratio of 3.6 (Refractive index=1.98) showed the best properties in after firing process condition. The single crystalline silicon solar cells fabricated according to optimized gas ratio (R=3.6) condition on large area substrate of size $156{\times}156mm$ (Pseudo square) was found to have the conversion efficiency as high as 17.2%. Optimized hydrogenated silicon nitride surface layer and high efficiency crystalline silicon solar cells fabrication sequence has also been explained in this study.

Leveling the Gamma-ray Spectrometric Data using Baseline Survey (Baseline 탐사를 이용한 항공 방사능 탐사 자료 맞추기)

  • Park, Yeong-Sue;Rim, Hyoungrea;Lim, Mutaek;Shin, Young Hong
    • Geophysics and Geophysical Exploration
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    • v.18 no.3
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    • pp.97-104
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    • 2015
  • KIGAM has surveyed most of the Korean territory since 1982 using airborne gamma-ray spectrometry, and complete the nationwide scale map in the near future. However, since the duration of survey is too long and the conditions of survey is not consistent, the data does not have physical consistency. In addition, the window counts (count/sec) were recorded instead of potassium, uranium and thorium radioelement concentrations. Thus, the data could not be registered to the International Atomic Energy Agency (IAEA) radioelement datum. This limits the usefulness of the data and it is not possible to easily combine surveys into regional compilations or make quantitative interpretations between different survey areas. To solve these problems, we undertook a test baseline survey over Jincheon-Eumseong area, to level the different two sets of data and to map radioelement concentrations. This survey confirms to IAEA radioelement baseline. The method and procedures of data leveling prepared by this study improve the usefulness and usability of the radiometric data, and make it enable to compile the nationwide scale radioelement concentration maps.

Development of Video Work Manual for Rock-Drill Data In Fire Service (소방에서의 도상훈련 기초자료 영상화작업 매뉴얼 개발)

  • Cho, Jae-Kwan;Park, Hee-Jin;Hwang, Inn;Kwon, Hayrran
    • The Korean Journal of Emergency Medical Services
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    • v.6 no.1
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    • pp.103-128
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    • 2002
  • As a result of trying the various manufacturing methods considering the reality of manpower and equipments with this manual, the following standardized procedures and contents can be suggested. (1) Since tools presenting Rock-Drill data must formalize the order of explanation although explainers are different, it will be valid that it is configured by existing power point method rather than by web document type. Composition of contents are selected on the basis of defence card and survey and then 8 items including initial screen, peripheral conditions, mobilization route, general conditions, use and structure by floor, department of vehicle consideration in activities and end screen are included. (2) Making methods and cautions of data included and used in power point are as follows ; - It was most effective that objects of fire fighting and location of neighboring fire fighting water were expressed by electronic map and drawing of inner building was made by scanning it after paining general architecture drawing(plan by each floor) rather than using drawing tools of EXCEL program or CAD drawing. And it was helpful to simplify contents of architecture drawing to wall, stairs and gate in understanding them. - Photographing of video data should be taken to show available fire fighting facilities in fire, use of planned space and the whole inner structure of each floor from the inside of fire fighting buildings and to display play time between 10 sec. and 1 min, for obstacles to distance from adjacent buildings or passage of special vehicles and fire fighting water from the outside of the building. - File format of video data taken in this way is most suitable to use wmv(window media video) or asf(advanced streaming format) type in consideration of time required for export, screen quality, file capacity and play type in Rock-Drill through network. - Still screen(photo) is more effective to express the department of fire fighting vehicles or other equipments than using video. (3) In configuration work of power point, hyper link was used most and configured to see any part at any situation like web document and then uniformity of presentation order of power point was complemented. (4) In case of sales facilities with the area of $35.557m^2$, the time of 22 hours and 30 minutes for five days was taken with five persons. Therefore, when eight-hour works a day were calculated, the whole process of video work for Rock-Drill can be finished with three day works.

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A Study on the Purchase of Foreign Brand Clothing according to Fashion Involvement. (유행몰입에 따른 해외 브랜드 의복 구매에 관한 연구)

  • 이선재
    • Journal of the Korean Society of Costume
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    • v.33
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    • pp.105-115
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    • 1997
  • The fashion clothing market is changing due to the appearance of new consumer group. And the import of foreign brand clothing is highly increasing. The purpose of this study is to help the domestic apparel companies set better market-ing strategy by studying the relation between fashion ivolvement and the purchase be-havior of foreign brand clothing. Subjects were 498 new generation women living Seoul metropolitan area. Data were obtained by questionnaire and analyzed by SPSS package. The main findings of this study are as follows; 1. The result of factor-analysis of the fashion involvement's variables were classified into 8 factors. 2. Consumer's main purpose of buying new clothes were To coordinate clothes with each other' 3. Consumers evaluated every clothing factors: color fit etc of foreign brand very highly except the price. 4. The factor that consumers consider most when choosing clothes is color design fit de-tail texture and brand knowledge in order. 5. the advertizing method that consumers care the most was shop and window display. 6. 'Because design and color are good' were the most important factor as motive of buying foreign brand clothing. 7. Most consumers still bought domestic brand clothing. 7. Most consumers still bought domestic brand clothing. 8. Monthly income and purchase of foreign brand were positively related 9. All variables 2of fashion involment were positively related with the purchase of foreign brand 10. Consumers with higher monthly income did not care much about 'Weather or occasion' when buying clothes. And consumers living in Kangnam(compared with consumers linving in Kangbuk) cared more about personality and bought more foreign brand. 'Social and econ-omic status' were highly related with monthly income residence and purchase of foreign brand clothing 11. Older consumers cared more about color figure texture and fit than younger con-sumer. Monthly income were positively re-lated with design color and figure. Residence were highly related with color and figure. Pur-chase of foreign brand clothing were positively related with design color figure and fit. 12. Younger consumers cared more about brand knowledge. And the purchase of foreign brand clothing were positively related with de-sign detail and brand knowledge. 13. Consumers with foreign brand's purchase experience showed negative relation between ;foreign brand's purchasing experience' and 'Weather or occasion' 14. Consumers with no foreign brand's pur-chasing experience showed negative relation between fashion innovativeness and figure. 15. Consumers with no foreign brand's pur-chasing experience showed positive relation between fit and fashion opinion exchange. The study shows that colors is most import-ant factor in purchasing clothes. And imported brands are very strong in terms of proposing various and unique colors. not all brands succeeded in Korea. Those brands that failed to group consumers have the following problems. Therefore it is critical to review the above factors when importing the foreign brand.

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Characteristics of InGaAs/GaAs/AlGaAs Double Barrier Quantum Well Infrared Photodetectors

  • Park, Min-Su;Kim, Ho-Seong;Yang, Hyeon-Deok;Song, Jin-Dong;Kim, Sang-Hyeok;Yun, Ye-Seul;Choe, Won-Jun
    • Proceedings of the Korean Vacuum Society Conference
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    • 2014.02a
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    • pp.324-325
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    • 2014
  • Quantum wells infrared photodetectors (QWIPs) have been used to detect infrared radiations through the principle based on the localized stated in quantum wells (QWs) [1]. The mature III-V compound semiconductor technology used to fabricate these devices results in much lower costs, larger array sizes, higher pixel operability, and better uniformity than those achievable with competing technologies such as HgCdTe. Especially, GaAs/AlGaAs QWIPs have been extensively used for large focal plane arrays (FPAs) of infrared imaging system. However, the research efforts for increasing sensitivity and operating temperature of the QWIPs still have pursued. The modification of heterostructures [2] and the various fabrications for preventing polarization selection rule [3] were suggested. In order to enhance optical performances of the QWIPs, double barrier quantum well (DBQW) structures will be introduced as the absorption layers for the suggested QWIPs. The DBWQ structure is an adequate solution for photodetectors working in the mid-wavelength infrared (MWIR) region and broadens the responsivity spectrum [4]. In this study, InGaAs/GaAs/AlGaAs double barrier quantum well infrared photodetectors (DB-QWIPs) are successfully fabricated and characterized. The heterostructures of the InGaAs/GaAs/AlGaAs DB-QWIPs are grown by molecular beam epitaxy (MBE) system. Photoluminescence (PL) spectroscopy is used to examine the heterostructures of the InGaAs/GaAs/AlGaAs DB-QWIP. The mesa-type DB-QWIPs (Area : $2mm{\times}2mm$) are fabricated by conventional optical lithography and wet etching process and Ni/Ge/Au ohmic contacts were evaporated onto the top and bottom layers. The dark current are measured at different temperatures and the temperature and applied bias dependence of the intersubband photocurrents are studied by using Fourier transform infrared spectrometer (FTIR) system equipped with cryostat. The photovoltaic behavior of the DB-QWIPs can be observed up to 120 K due to the generated built-in electric field caused from the asymmetric heterostructures of the DB-QWIPs. The fabricated DB-QWIPs exhibit spectral photoresponses at wavelengths range from 3 to $7{\mu}m$. Grating structure formed on the window surface of the DB-QWIP will induce the enhancement of optical responses.

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Study on Application of Ultrasonic Propagation Imager for Non-destructive Evaluation of Composite Lattice Structure (복합재 격자 구조 비파괴평가를 위한 초음파전파 영상화 시스템 활용 연구)

  • Park, Jae-Yoon;Shin, Hye-Jin;Lee, Jung-Ryul
    • Composites Research
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    • v.30 no.6
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    • pp.356-364
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    • 2017
  • Composite lattice structures are tried to be used in various fields because of its benefit in physical properties. With increase of demand of the composite lattice structure, nondestructive testing technology is also required to certificate the quality of the manufactured structures. Recently, research on the development of the composite lattice structure in Republic of Korea was started and accordingly, fast and accurate non-destructive evaluation technology was needed to finalize the manufacturing process. This paper studied non-destructive testing methods for composite lattice structure using laser ultrasonic propagation imaging systems. Pulse-echo ultrasonic propagation imaging system was able to inspect a rib structure wrapped with a skin structure. To reduce the time of inspection, a band divider, which can get signal in different frequency bands at once, was developed. Its performance was proved in an aluminum sandwich panel. In addition, to increase a quality of results, curvature compensating algorithm was developed. On the other hand, guided wave ultrasonic propagation imaging system was applied to inspect delamination in a rib structure. To increase an area of inspection, multi-source ultrasonic wave propagation image was applied, and defects were successfully highlighted with variable time window amplitude mapping algorithm. These imply that ultrasonic propagation imaging systems provides fast and accurate non-destructive testing results for composite lattice structure in a stage of the manufacturing process.

A Study on the Spatial Structure of the rural Dwelling Houses of China's Korean Ethnic - Based on Investigating and analyzing each District's rural Dwelling houses of China's Three Northeastern Provinces - (조선족 농촌주거 공간구성형태의 지역적 특징에 관한 연구 - 중국 동북3성 각 지역의 조선족 농촌주거에 대한 조사연구를 중심으로 -)

  • Jin, Ri-Xue;Park, Yong-Hwan
    • Journal of Korean Society of Rural Planning
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    • v.15 no.4
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    • pp.75-87
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    • 2009
  • This paper tries to explore the local uniqueness and evolutionary characteristics of the spatial structure of the rural dwelling houses of China's Korean Ethnic. For this purpose, the paper selected 51 typical samples in each district, analyzing their ancestral home, building age, size, construction method, family composition etc. Moreover, the paper classified the pattern of the samples and analyzed the characteristic and evolutionary process of each pattern. Conclusions based on the analysis of the ancestral homes are as follows: 1.The house pattern of these China's Korean ethnics who are originally from the Hamkyung province is mainly jeongji- access type (A-type). A-type is mainly distributed along the Tumen River and Yalu River, in the Sino-Russian borders and the inland areas of Heilongjiang province. With the migration of the China's Korean ethnics, in Sino-Russian borders and the inland areas of Heilongjiang province, A-type with the 'badang' space, vestibule-access type (C-type) and living-centered type (D-type) appeared. 2. House patterns of Korean ethnics who are originally from the Pyongan province include three types: A-type, kitchen type (B-type) and C-type. A-type is mainly distributed along the Yalu River while B-type and C-type are mainly in the inland areas of these three Northeast provinces. With the decrease of population, the merger of rooms happened in A-type; while in the B-type and C-type, bathroom and storage came to exist in the north of the room. 3. The house pattern of Korean ethnics who are originally from the Gyeongsang province is mainly B-type, which is distributed in the inland areas of Heilongjiang and Jilin provinces. Besides, C-type and D-type also exist. They are in Jilin and Liaoning provinces. In the process of evolution, storage was set in the north part of the room or the window was cancelled in order to defend the coldness in Heilongjiang area, while in Jilin and Liaoning provinces, living room came into existence, which is gradually developing to D-type.