• 생명과학회지
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• 제28권9호
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• pp.1118-1126
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• 2018

본 논문은 양식 어류에게 많은 경제적 피해를 입히고 있는 다양한 어류질병세균 중에서 연쇄구균증에 대한 일반적인 특성과 종류, 진단 방법에 대하여 기존에 연구 보고된 논문을 기반으로 알아보고자 한다. 대표적인 어류연쇄구균증의 원인체로는 Lactococcus garvieae, Streptococcus iniae, S. parauberis가 있다. 연쇄구균증에 감염 시 나타나는 증상으로는 체색변화, 안구이상, 아가미 퇴색, 출혈, 복부팽만, 신장과 비장의 종대 등의 보이다 폐사가 일어난다. 또한 수온이 상승하는 6월부터 10월까지 주로 일어나며 집단적으로 발병하여 폐사가 일어난다. 현재 연쇄구균증을 진단하기 위한 기술로는 16S rRNA, 16S-23S rRNA intergenic spacer region (ISR), Random Amplified polymorphic DNA (RAPD), Ribotyion (RT), Loop-mediated isothermal amplification (LAMP) 등이 있다. 이중에서 현장에서 적용 가능성이 높은 LAMP법이 각광을 받고 있지만 결과 확인 등의 문제로 인하여 현재는 어려움을 겪고 있는 실정이다. 이에 현장에서 진단부터 결과 확인까지 손쉽게 사용할 수 있도록 문제점을 보완하면 연쇄구균증에 대한 경제적 손실을 최소화 할 것으로 사료된다.

• 한국양식학회지
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• 제20권1호
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• pp.51-55
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• 2007

이 연구는 실용 배합사료를 자체 제작한 기존의 배합사료, 상품사료 및 습사료를 장기간 걸쳐 사육한 넙치에 대해 육질을 평가하기 위해 수행하였다. 실험어는 13개월간 사육한 넙치 성어기(600 g 전후) 대상으로 수행하였으며, 실험사료는 시험사료(EP1 및 EP2) 2종, 상품사료(CEP) 및 습사료(MP)를 공급하였다. 전어체의 성분분석은 조단백질은 EP2가 EP1 및 CEP 사료구 보다 유의적으로 높게 나타났으며, 조지방은 EP2 및 MP 사료구가 CEP 사료구와 비교하여 유의적으로 높게 나타났다. 근육내 유리아미노산의 성분분석은 EP사료구간에서 MP 사료구보다 proline, glycine, alanine 함량이 높게 나타났으며, taurine에 있어서 EP1 사료구가 다른 사료구와 비교하여 높은 경향을 보였다. 핵산 관련물질 성분분석은 ATP 및 IMP의 양에서 배합사료에서 습사료보다 높은 값을 보였으며, 특히 EP2에서 다른 사료구와 비교하여 높은 값을 보였다. 관능검사는 향, 외관, 맛에 있어서 EP 사료구와 MP 사료구가 유의적인 차이를 보이지 않았다(P>0.05). 전반적인 기호도에 있어 EP2가 다른 사료구들보다 유의적으로 높게 나타났다. 물리적 성질의 경도 측정은 EP2는 EP1 및 CEP 사료구와 비교하여 높게 나타났으며, 탄력성과 응집성은 모든 사료구에서 차이를 보이지 않았다. 상기 결과를 토대로 그동안 배합사료를 넙치에 공급한 연구 결과를 고려하였을 때, 생사료를 공급한 넙치에 비해 품질이 결코 떨어지지 않으며, 오히려 배합사료를 공급한 양식산 넙치가 체계적인 관리 하에 앞으로 품질향상을 더 높일 수 있을 것이다.

• 한국수산과학회지
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• 제50권5호
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• pp.547-552
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• 2017

The viral hemorrhagic septicemia virus (VHSV) has an extensive host range, and infects farmed and wild fish inhabiting both freshwater and marine ecosystems. Enzyme-linked immunosorbent assay (ELISA) is highly useful in diagnosing viral hemorrhagic septicemia. However, ELISA shows high, non-specific background reaction with fish antibodies. In this study, we optimized the antigen and antibody concentrations used for detecting specific antibodies in VHSV-infected olive flounder to reduce non-specific binding, and improve the sensitivity of ELISA. The results suggested that OD (optical Density) values were valid when ELISA was performed with $0.1{\mu}g/well$ of virus, involving blocking with blocking buffer (Roth, Roti-Block), 1:300-1:600 dilution with flounder antisera, and 1:1000 dilution with anti-flounder IgM and HRP-conjugated goat anti-mouse IgG for detecting the VHSV antibody in flounder sera. Furthermore, 11 different VHSV strains isolated in Korea from 2012 to 2016 were used to infect the fish. The results showed no correlation between viral pathogenicity and antibody production. This research is a basic study on the application of antibody detection in the diagnosis of viral hemorrhagic septicemia in the olive flounder.

• 한국수산과학회지
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• 제50권3호
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• pp.287-295
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• 2017

Egg development and morphological change of larvae of the Sakhalin sole Limanda sakhalinensis were studied by observing specimens obtained in a rearing experiment from fertilized eggs to the juvenile stage. The wild broodstock was collected in January 2010 and kept in a circular water tank (${\O}1.5{\times}1m$) at a temperature of $14.5{\pm}0.5^{\circ}C$. Fertilized eggs ranged from 0.72 to 0.82 mm ($0.77{\pm}0.07mm$, $mean{\pm}SD$) in diameter. The eggs were spherical, transparent and adhesive demersal. The egg yolk was divided from the oocyte 10 min after fertilization (AF), and an embryo was formed in 36 h AF. More than 50% of the eggs hatched within 133 h AF. The mouth and anus did not open until $3.5{\pm}0.25mm$ total length (TL). At 4, days after hatching (AH), the fish became larvae 3.7 to 4.2 mm ($4.0{\pm}0.36mm\;TL$), yolk absorption was completed and the mouth began to open. The left eye moved upward and the nostril moved to the right at 39 days AH. These post-larvae ranged from 8.0 to 9.9 mm TL ($8.9{\pm}1.33mm\;TL$). At 50 days AH, the fish became juveniles ($12.4{\pm}1.20mm\;TL$) There were 70-72 dorsal fin rays, 55-56 anal fin rays, 11 pectoral fin rays, and 6 ventral fin rays and the juveniles adopted a benthic life.

• 환경영향평가
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• 제20권5호
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• pp.685-695
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• 2011

Through the analyses of the distribution characteristics and management practices of the wildlife protection areas, this study intends to present basic data for improving the management of protected areas. 1) Based on the aggregate results of the protected areas, the average ratio of the metro-city and provincial areas to the designated protected areas was 1.49%; except for Chungcheongbuk-do (8.83%) and Gyeongsangnam-do (3.43%), most regions showed specified percentages of about 1%, whereby the analysis suggested that the designated protected areas were too small. 2) Considering the fact that most (86.16%) of the wildlife protection areas are in the regions that are of ecological nature level of grade 1, green nature level of grade 8 or better, and of legal conservation regions and districts, it was shown that these reserves are distributed in regions with excellent natural environmental conditions. 3) Designation of protected areas for the protection of wildlife and fish is deemed to be necessary. Although there are 588 wildlife & plant reserves, there are absolutely no designated areas for protecting plant and fish species. 4) Based on the results of an analysis of the current distribution by types, 92.47% of protected areas were concentrated in the mountains, 0.24% in the agricultural lands, and 7.29% distributed in the lakes/rivers/costal areas. 5) As major improvement projects, proposed were: advancement of management systems for protected areas, expanded designation of protected areas, overhaul of managed areas, designation of protected areas for the protection of wild plants and fish, need for management measures for private property and the surrounding areas, need to secure appropriate management personnel and collaboration, locally-adapted management, eco-information-based management, total management of protected areas, etc.

• 현장농수산연구지
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• 제25권3호
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• pp.35-46
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• 2023

It was in the 1982 that artificial seed production research for olive flounder (Paralichthys olivaceus) farming was first conducted in Korea (Currently, National Institute of Fisheries Science, Fish Breeding Research Center). In 1985, fertilized eggs were obtained from natural olive flounder adapted to land tanks, and artificial seed production technology was established and fertilized eggs were distributed. In the late 1980s, halibut aquaculture began to prosper in land-based tank farming in Jeju Island and Busan's Gijang region, where water temperatures are relatively high in winter. Currently, aquaculture is being carried out all over the country, centering on Jeju Island and Wando, Jeollanam-do. However, olive flounder farming, which started with a small group in the 1980s, reduced genetic diversity through inbreeding over generations, resulting in side effects such as slow growth, reduced resistance to disease and environmental conditions. In order to solve these genetic problems of farmed olive flounder in Korea, the Fish Breeding Research Center of the National Institute of Fisheries Science introduced a wild-caught parent fish group to the existing aquaculture group from 2003 to 2004. Genetic diversity was secured and KingNupchi with fast growth and improved body shape was developed. In this study, the current status of breeding technology development of olive flounder, a major aquaculture breed in Korea, is reviewed and future research directions are suggested.

• 생명과학회지
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• 제26권8호
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• pp.955-962
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• 2016

본 연구에서는 연제품용 어육 원료의 안정적인 수급과 고품질 연제품 개발을 위한 방안으로 우리나라에서 주로 양식되고 있는 도미를 이용하여 제조한 연육의 어육 겔 특성을 측정하여 도미의 연제품 소재로서의 가능성을 검토하였다. 현재 우리나라에서는 연제품 제조에 이용되는 어육원료는 주로 수입에 의존하고 있으며 국내에서는 저가의 연육이 일부 생산되고 있다. 수입 연육의 경우 대부분 동남아 및 북아메리카로부터 수입한 냉동 연육이 사용되고 있다. 연육의 품질과 등급은 수분함량, 백색도, 겔 특성 등에 의해 결정되어진다. 따라서 본 연구에서는 양식어종인 도미(Pagrus major)를 원료로 하여 전통 수세법으로 연육을 제조하였으며, 도미 유래 연육을 이용하여 어육겔 형성능 및 품질 특성을 겔 강도, 텍스쳐 실험, 백색도, 수분유출정도 및 SDS-page pattern 측정을 통해 검토하였다. 이들 결과는 FA급과 RA급의 시판용냉동명태연육을 이용하여 제조한 어육 겔 특성과 비교하였다. 겔 특성을 검토하기 위해 연육에 2% NaCl를 첨가하여 전체 수분함량이 80%가 되도록 하여 90℃에서 20분간 가열한 소시지 형태의 어육 겔을 제조하였다. 도미 유래 연육의 어육 겔 특성은 stress-strain curve로부터의 겔강도, cutting strength, breaking force 등의 결과 및 텍스쳐 실험을 통한 springiness, cohesiveness, chewiness, brittleness 등의 결과로부터 확인할 수 있었다. 도미 유래 연육의 어육 겔 특성은 시판용 명태연육(FA급과 RA급)의 어육 겔 특성과 비교하였다. 도미연육의 사용은 RA급의 명태연육에 비해 높은 겔 형성능을 나타내었으며, FA급의 명태연육과는 비슷한 정도의 겔 특성을 나타내었다. 이상의 결과로부터 도미를 이용한 고품질 연제품의 개발 가능성을 확인할 수 있었다.

• 한국환경과학회지
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• 제17권8호
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• pp.827-831
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• 2008

We investigated the education process of an environmental experiment in middle and high school. The major objective was that students understand about biological toxicity of endocrine disruptor. We used bisphenol A in environmental experiment to educate them about the effects of endocrine disruptor. The test animal was zebrafish (Danio rerio, wild type) which is very easy to use in the school experiment class. We educated the high school students (n=25) in Suwon through this teaching program. Finally we analyzed the effect of research education between two groups, education students (test) and non-education students (control). This experiment showed there were some positive effects on environmental reorganization of students. They understood the affect of endocrine disruptors in fish and man. Specifically, understanding the importance of preservation of ecosystem increased in the test-education group.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2003년도 춘계 수산관련학회 공동학술대회발표요지집
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• pp.315-317
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• 2003

Penaeid shrimp (Penaeus chinensis), economically important aquacultural species, belonging to family Penaeidae including genus Fenneropenaeus, widely inhabit the West Sea and South Sea in the Korean Peninsula and the Gulf of Pohai in China under the natural ecosystem. Consequent of the rapid increase in seed production, there is a need to understand the genetic traits and composition of this fish species in order to evaluate exactly the patent genetic effects induced by seed production operations. (omitted)

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2003년도 춘계 수산관련학회 공동학술대회발표요지집
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• pp.158-159
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• 2003

Although the sub-chronic toxic effects of metal on fishes are well documented, that is mostly in fishes exposed to waterborne metal but few studied have been conducted on the effects of dietary metal (Handy, 1996). The realization that dietary uptake of metal is a major cause of long-term contamination in wild fish has renewed interest in the nutritional and toxicological effects of metal in the food of fishes (Handy, 1996). (omitted)