• 한국작물학회지
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• 제47권2호
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• pp.116-122
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• 2002

Dehydrins (LEA Dll proteins) are one of the typical families of plant proteins that accumulate in response to dehydration, cold stress, abscisic acid, or during seed maturation. A 1.3-kb cDNA was cloned from a cDNA expression library of 5-day-old germinating maize scutellums under drought stress. The deduced protein sequence indicated a dehydrin gene encoding SK$_3$ LEA protein typically expressed during cold acclimation, but not by drought stress in barley and wheat. Thus, it was named maize DEHYDRIN2 (ZmDhn2). It accumulates rapidly and highly in drought-stressed scutellum and leaf tissues at any stage, but not under cold stress. ZmDhn2 gene was transformed into Arabidopsis thaliana for functional analysis under drought condition. From electrolyte leakage test, no significant difference showed between wild type and transformants under normal growth condition, but the leakage level of electrolyte in wild type plants was about 3 times as high as that in the transformed plants under drought stress. It suggests that ZmDHN2 playa role in increasing drought tolerance.

• Journal of Applied Biological Chemistry
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• 제43권1호
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• pp.12-17
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• 2000

To understand the signal transduction pathway that leads to the activation of the wound-inducible proteinase inhibitor II (pin2) promoter. $F_2$ progenies of wound (-) mutant crossed with wild-type Arabidopsis plants were biochemically and genetically characterized. Wound (-) mutant was derived from transgenic Arabidopsis plants containing bacterial cytosine deaminase gene under the control of pin2 promoter. The cytosine deaminase assays indicated that wound (-) mutant is a dominant inhibitor of wound-inducibility as only 3 of the $20F_2$ progenies showed cytosine deaminase (CDase) activity, To construct a structural map of the wound (-) mutant chromosomal regions, cleaved, amplified polymorphic sequences (CAPS) markers that cover all Chromosomes were used. Chromosomal regions covered by three different CAPS markers could be candidates for further fine mapping of the location of the wound (-) mutation. g4026, RGA1 and ASA1 located at 84.9 on recombinant inbred (RI) map of chromosome I, at 1.75 on RI map of chromosome II, and 18.35 on RI map of chromosome V, respectively.

• Journal of Plant Biotechnology
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• 제5권4호
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• pp.201-207
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• 2003

Tissue culture techniques arguably are an important approach for ex situ conservation of rare and endangered plant species. However, there is utmost importance on maintaining the genetic integrity of the introduced plants especially in tree species. To examine the genetic integrity of the micropropagated plants, we randomly screened few hardened plants of Syzygium travancoricum, a critically endangered tree taxon, using Randomly Amplified Polymorphic DNA (RAPD) markers. Twenty-three random. primers were tried and twenty-five polymorphic loci were identified. The dendrogram based on the Unweighted Pair-Group Method Arithmetic Average and Nei's similarity index depicted about 97% homology between the mother plants and micropropagated plants. Further, an attempt was made to reintroduce the micropropagated plants in the wild. Over three hundred small trees could be successfully established.

• 한국조경학회지
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• 제18권3호통권39호
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• pp.57-70
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• 1990

This study is on system building and model case study to establish a new framework of "The Wild and Scenic Rivers System" and to apply this to Korean rivers. The Wild and Scenic Rivers System of the U. S. , which had become law in 1968 for establishing wild scenic and recreational river areas, was investigated. The techniques of two American case studies about the National Wild and Scenic Rivers System designation were investigated and synthesized, and study process was adjusted to Korean river system. Additionally, pilot study was carried out by the method and results were as follows : 1) In the American Wild and Scenic Rivers System, each river was evaluated based on eligibility criteria for designation, such as remarkable scenic, recreational, fish and wildlife, cultural or other ecological values. Segmented river areas were classified into three or four grades and management plans were prepared accordingly. 2) The management of rivers in Korea has emphasized on the flood control and water resources management up to the present, and now takes a growing interest in water quality. But it has been concerned very little with wild and scenic resources conservation of river corridors. It is strongly recommended to build Wild and Scenic Rivers System in Korea for rational management and conservation of the valuable natural resource. 3) Suggested evaluation criteria for the Korean Wild and Scenic Rivers System were wildness, wildlife and plants, hydrology, scenic quality, and historical and cultural resources. The river areas may be graded into four : Positive conservation area(I), Negative conservation area(II), Negative development area(III), Positive development area(IV). Management guidelines were proposed for each grade. 4) To test the applicability of the approach, one of the major tributary of the Han-River was selected for pilot study. The result showed that the evaluation and grading system worked well. Finally, it is recommended to incorporate the Wild and Scenic Rivers System into Korean national park system, and to amend the Korean National Park Act to enable this.

• 식물병과 농업
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• 제5권1호
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• pp.58-60
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• 1999

Cultivation of a native wild vegetable, Ligularia fischeri, is hampered by Phytophthora sp. Infected plants showed wilt and eventual death due to rots on the root and basal portion. Eight isolates collected were all identified as P. drechsleri based on their mycological characteristics. The fungi showed relatively strong pathogenicity to L. Fischeri, mild to Aster scaber and Codonoposis lanceolata, and none to Circium setidens and Pimpinella brachycarpa. This is the first report of Phytophthora root rot on wild vegetables in Korea.

• 한국환경복원기술학회지
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• 제4권3호
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• pp.84-95
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• 2001

This study was conducted to suggest the ecological restoration methods for the decomposed granite roadside cutslopes by Natural Topsoil Restoration Methods(NTRM). Four woody species, 3 shrub species, 4 native grasses, 8 wild flowers and 2 cool-season grasses were used. The experiment was conducted with the purpose of developing ecological restoration techniques by achieving following objectives for restoration by using native woody plants, shrubs, native plants and wild flowers; (1) prevention or reduction of wind and water erosion, (2) provision of food and cover for variety of animal species, (3) improvement of the visual or aesthetic quality of disturbed sites. The main results were summarized as follows. 1. Restoring naturalness to human-altered landscapes are important to protecting the worlds biodiversity. In this study, to restore or create an ecological community use as full a complement of native species as possible. Ecological restoration means returning a specific sites to its predisturbance condition. 2. Some species which are introduced first may inhibit the germination and growth of slower-growing species, or they may prevent the growth of other species whose propagules arrive later. In this study, cool season grasses was used small amount than native plants and woody species, because and Indigofera pseudo-tintoria grows well. Tall fescue and perennial ryegrass shows quick germination, but they could not inhibit the other species growing relatively slow. Pinus thunbergii used at Gonam near the ocean was suppressed by the other plants, so it can be concluded that using container seedlings is effective than seeding. 4. Ailanthus altissima, Albizzia julibrissin, Rhus chinensis Lespedeza cyrtobotrya, and Indigofera pseudo-tinctoria were germinated. In wild flowers, Coreopsis lanceolata, Dianthus sinensis, Coreopsis tinctoria, and Lotus corniculatus var. japonicus grew well and show enough plant height which will not suppressed by shrub and woody plants. After one year, native grasses such as Arundinella hirta, Miscanthus sinensis var. purpurascens and Imperata cylindrica var. koenigii cover the slopes well. 5. 25 herbaceous plants such as Trifolium repens, Arthraxon hispidus, Barbarea orthocerae, Potentilla fragarioides, Lepidium apetalum, Chenopodium album var. centrorubrum, Glycine soja, Rubus crataegifolius and 4 woody species such as Lespedeza maximowiczii, Robinia pseudoacacia, Symplocos Chinensis, Quercus acutissma were invaded. 6. Ecological restoration works by Natural Topsoil Restoration Methods for revegetation of the decomposed granite roadside cutslopes were successfully accomplished. It prevents surface water erosion and shows diverse vegetation structure. It will be successful to restore decomposed granite cutslopes.

• 한국자원식물학회지
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• 제22권1호
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• pp.13-25
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• 2009

본 연구는 속리산국립공원 내 쌍곡계곡에 분포하는 식물상을 조사하기 위해 수행되었고 조사기간은 2006년 3월부터 2007년 6월까지 조사하였다. 식물상을 조사한 결과, 88과 242속 342종 52변종 7품종 등 총 401분류군이 확인되었다. 한국특산식물은 개비자나무, 키버들, 개족도리, 할미밀망, 민산초나무, 청괴불나무, 병꽃나무, 분취 등 총 8분류군이 조사되었다. 환경부 지정 멸종위기식물은 망개나무 등 1분류군이며, 산림청 지정 희귀식물은 개족도리, 고란초, 망개나무, 백작약, 태백제비꽃, 꼬리진달래, 말나리 등 7분류군이 조사되었다. 이들의 보전을 위해서는 향후 희귀식물에 대한 구체적인 보전방안과 번식방법 등이 논의되어야 할 것으로 판단된다. 자원식물은 관상용 식물 220분류군, 식용 식물 239분류군, 약용 식물 281분류군, 기타용 식물 206분류군으로 나타났다. 귀화식물은 오리새, 소리쟁이, 취명아주, 아까시나무, 붉은토끼풀, 토끼풀, 큰달맞이꽃, 비짜루국화, 붉은서나물, 개망초, 망초, 뚱딴지, 서양민들레 등 13분류군이 관찰되었으며, 귀화율은 약 3.24%, 자연파괴도는 약 4.53%로 나타났다.

• 한국자원식물학회지
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• 제35권1호
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• pp.44-70
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• 2022

본 연구는 서산시 팔봉산의 식물자원 분포 및 특성을 파악하고, 선행연구와의 비교를 통해 팔봉산 식물자원 보전을 위한 기초자료로 제공하기 위해 수행되었다. 조사는 2018년 4월부터 2021년 5월까지 11회에 걸쳐 경로를 구분해 관속식물의 분포를 파악하였다. 그 결과 확인된 관속식물은 101과 298속 405종 11아종 28변종 6품종으로 총 450 분류군이었다. 이 중 특산식물은 8종류였고, 희귀식물은 4 분류군이 관찰되었다. 식물구계학적 특정식물은 총 30 분류군으로 IV등급에 2 분류군, III등급에 6 분류군, II등급에 7 분류군, I등급에 15 분류군이 포함되어 있었다. 귀화식물은 39 분류군이 관찰되었고, 귀화율은 8.67%로 산출되었으며, 도시화지수는 12.15%로 계산되었다. 생태계교란 야생식물은 5 종류가 확인되었다. 귀화식물과 생태계교란 야생식물은 주로 임도와 밭경작지, 숲 가장자리에서 발견되므로 분포현황에 대한 지속적인 모니터링과 주기적인 식생 관리가 이뤄져야 할 것으로 판단된다.

• 한국환경복원기술학회지
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• 제16권2호
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• pp.75-92
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• 2013

The vascular plants of Thuja orientalis community in Seokmun-bong, Danyang were listed 222 taxa (4.5% of all 4,881 taxa of vascular plants); 67 families, 150 genera, 163 species, 2 subspecies, 43 varieties and 14 forms. Limestone plants, 29 taxa; Asplenium rutamuraria, Celtis koraiensis, Goodyera coreana, etc. So, Based on the legal protection species, endangered wild plants II, were recorded; Thalictrum coreanum (EN) and rare plants, 8 taxa; Thuja orientalis (LC), Exochorda serratifolia var. serratifolia (LC), Diarthron linifolium (EN), Bupleurum falcatum (VU), Lithospermum erythrorhizon (LC), Dracocephalum argunense (EN), Polygonatum robustum (EN), etc. and endemic plants, 3 taxa; Populus tomentiglandulosa (Planted plant), Thalictrum actaefolium var. brevistylum, Asperula lasiantha, etc. Based on the list of specific plants, 29 taxa; Thalictrum coreanum in class V, 7 taxa (Neillia uyekii for. uyekii, Prunus sibirica, Abelia coreana, etc.) in class IV, 8 taxa (Spiraea trichocarpa, Polygala tatarinowi, Asparagus oligoclonos, etc.) in class III, 2 taxa (Potentilla dickinsii var. dickinsii, Weigela florida) in class II, 11 taxa (Morus cathayana, Buxus microphylla var. koreana, Zizyphus jujuba var. jujuba, etc.) in class I. Based on the list of naturalized plants, 10 taxa (Robinia pseudoacacia, Oenothera biennis, Festuca arundinacea, etc.), naturalization rate (NR) was 4.5% of all 222 taxa of vascular plants and urbanization index (UI) was 3.1% of all 321 taxa of naturalized plants.

• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 2000년도 추계심포지움 및 학술발표회:바이오모니터링 기법을 이용한 환경위해성 평가
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• pp.65-65
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• 2000