• Journal of Radiation Protection and Research
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• 제25권4호
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• pp.217-221
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• 2000

소화기계 암의 효과적인 치료중의 하나로 방사선 치료법을 들 수 있으나 이 방법은 장 점막과 같이 빠르게 증식하는 조직에는 특히 부작용을 초래할 수 있다. 생쥐의 공장 융모가 감마선 전신 조사에 의해 형태학적으로 변화하는 정도를 관찰하였다. 생후 4주된 ICR계 생쥐를 6.5Gy와 12Gy를 전신 조사 3일 후에 공장을 채취하여 탈수 과정을 거쳐 파라핀을 입힌 조직을 절편기로 잘라 슬라이드에 부착시키고 일반적인 hematoxylin & Eosin 염색과정을 거쳐 염색후 광학현미경으로 관찰한 결과, 6.5Gy와 12Gy를 조사 받은 생쥐의 융모는 대체로 정상생쥐와 비교 할 때 융모의 길이가 짧아졌으며 융모의 두께가 비후되었고 내강은 넓어지는 경향을 나타내었다. 따라서 융모가 소화과정에 중요한 역할을 담당하며 방사선에 비교적 민감함을 나타내므로 방사선에 의한 생체영향 평가 뿐만아니라 방사선 방어제를 검색하는데 하나의 실험 파라메타가 충분히 될 수 있음을 인지하였다.

• 대한수의학회지
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• 제34권4호
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• pp.665-678
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• 1994

This study was undertaken to investigate histological changes according to the radiosensitivity in the spermatogenic cells in Korean native pheasants. During spermatogenetic period, testes wete collected from male adult Korean native pheasant and they were used as experimental and control birds. The experimental group was divided into a single-dose whole body irradiation group(400, 600, 800 and 1000 rads) and a split-dose whole body irradiation groups(400/2, 600/2, 800/2 and 1000/2 rads). A Henseky's $^{60}Co$ ${\gamma}$-radiotherapy machine was used for this experiment and the dose rate of $^{60}Co$ ${\gamma}$-ray was 104 rads/min. The experimental birds were sacrificed at 24 and 72 hrs after irradiation and the control pheasants were sacrificed at the same time. General histological changes of seminiferous epithelial cells were observed by hematoxylin-eosin stain with light microscope. The results obtained are summarized as follows ; 1. In the single-dose and the split-dose irradiation groups, the average diameter of the seminiferous tubule was decreased compared with control group. 2. Seminiferous epithelial cells were more severely damaged after 72 hrs than after 24 hrs of single-dose irradiation of 400, 600 and 800 rads but the difference of cell injury was almost not observable with the elapsed time in the group of the single-dose irradiation of 1000 rads. 3. The damage of spermatogenic cells were more severe after 24 hrs than after 72 hrs of the split-dose irradiation of 400 rads but the split-dose irradiation of 600, 800 and 1000 rads were more severe after 72 hrs than after 24 hrs.

• Journal of Microbiology and Biotechnology
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• 제27권12호
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• pp.2237-2240
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• 2017

In the present study, we investigated the effect of tyndallized HY7712 (tHY7712) on the expression of Th cell specific transcription factors and cytokines in whole-body ${\gamma}$-irradiated mice. Oral administration of tHY7712 strongly recovered the ${\gamma}$-irradiation-suppressed expression of helper T (Th) cell- and regulatory T cell-related transcription factors and cytokines, such as T-bet, Foxp3, IFN-${\gamma}$, TNF-${\alpha}$, and IL-10, and suppressed Th2 cell-associated transcription factor and cytokine GATA3 and IL-5, respectively. Furthermore, compared with the control, tHY7712 treatment also restored ${\gamma}$-irradiation-impaired natural killer and cytotoxic T cell activities against YAC-1 tumor cells to 97.8% and 98.6%, respectively.

• 대한수의학회지
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• 제42권2호
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• pp.183-190
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• 2002

The effects of ionizing radiation on the peripheral blood elements of ICR mouse were examined after varying doses of whole-body gamma-irradiation. ICR mice (n=50) were exposed to 0, 2, 4, 6 and 8 Gy gamma-ray ($^{60}Co$) at 10 Gy/min. The animals were studied for their hematological response on days, 3, 7, 14, 21, 42 and 56 post irradiation. No significant change was noted in erythrocyte, hemoglobin and hematocrit values after irradiation with dose of 2 Gy. Decreasing erythrocyte, hemglobin and hematocrit values occured after irradiation with doses of more than 4 Gy on day 7 after irradiation followed by a sharp fall on day 14. A recovery in these values was noted after 3 weeks of irradiation. Thrombocyte counts decreased on day 3, reaching minimal values on day 7. The total number of leukocytes was reduced on day 3, mainly because of a decrease in the lymphocyte population. An evident lymphopenia and neutropenia occur almost on the day 3 and last up to the day 28 after irradiation. All of the hematological values decreased in the blood in a dose-dependent manner at the same time.

• Advances in Traditional Medicine
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• 제6권1호
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• pp.12-20
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• 2006

Some natural products are able to inhibit radiation effects and exert an antitumor effect with fewer adverse reactions; however, their antitumor effects are less than those of widely-used synthetic drugs. Propolis is a natural material that has been attracting attention, and we extracted this material with water and investigated the effect of continuous propolis administration on radioactivity-induced reduction of hemocytes, in addition to the antioxidant and antitumor effects of propolis. Following a 1-week adjustment period, water-soluble propolis was administered intraperitoneally to male ICR mice at a dose of 100 mg/kg every other day for 2 weeks. Following administration, 2 Gy whole-body irradiation was performed and the counts of leukocytes, lymphocytes, and granulocytes and monocytes in the peripheral blood were determined 1, 3, 7, 15 and 30 days after irradiation. These cells were considered since they are closely associated with immunity to radioactivity. In a second experiment, water-soluble propolis was similarly administered to the mice for 2 weeks after a 1-week adjustment period, and 2 Gy whole-body irradiation was performed. The antioxidant effects in hemocytes were then investigated using 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH), a radical generator. In a third experiment, $1\;{\times}\;10^6$ Sarcoma-180 cells were inoculated into the right thigh of mice, which were divided into four groups: control, water-soluble propolis-treated, 6 Gy irradiated and water-soluble propolis-treated + 6 Gy irradiated groups, and changes in tumor size were measured for 20 days. Statistical analysis was conducted using ANOVA for multiple groups. In the three experiments, administration of water-soluble propolis inhibited the reduction of hemocytes caused by whole-body irradiation, showed antioxidant effects against radioactivity, and inhibited tumor growth, respectively. In conclusion, our data suggest that the antioxidant effect of watersoluble propolis inhibits hemocyte reduction caused by whole-body irradiation and enhances immunological inhibition of tumor growth.

• 한국원자력학회:학술대회논문집
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• 한국원자력학회 2001년도 춘계학술발표회요약집
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• pp.397-397
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• 2001

• Journal of Radiation Protection and Research
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• 제40권3호
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• pp.187-193
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• 2015

Deleterious effects of high dose radiation exposure with high-dose-rate are unarguable, but they are still controversial in low-dose-rate. The regulator of G-protein signaling (RGS) is a negative regulator of G protein-coupled receptor (GPCR) signaling. In addition, it is reported that irradiation stress led to GPCR-mediated mitogen-activated protein kinase (MAPK) and phosphotidylinositol 3-kinase (PI3-k) signaling. The RGS mRNA expression profiles by whole body radiation with low-dose-rate has not yet been explored. In the present study, we, therefore, examined which RGS was modulated by the whole body radiation with low-dose-rate ($3.49mGy{\cdot}h^{-1}$). Among 16 RGS expression tested, RGS6, RGS13 and RGS16 mRNA were down-regulated by low-dose-rate irradiation. This is the first report that whole body radiation with low-dose-rate can modulate the different RGS expression levels. These results are expected to reveal the potential target and/or the biomarker proteins associated with male testis toxicity induced by low-dose-rate irradiation, which might contribute to understanding the mechanism beyond the testis toxicity.

• Journal of Radiation Protection and Research
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• 제9권2호
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• pp.67-75
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• 1984

As a part of studies on acute effects of high dose irradiation the present report was carried out to evaluate the changes of the leukocyte life span in the Newzealand white male rabbits by a single whole body exposure to gamma rays from $^{60}Co$ teleirradiation unit. The exposure was done in dose levels of 100, 300, 550 and 1,000 rad to each experimental group of 10 rabbits. The life span and apparent half survival time of leukocytes, and the elution rate of leukocytes in the circulating blood were measured by McMillan method using $^{51}Cr$. 1. As a critical indicator of radiation hazards of the Newzealand male rabbits, the LD 50/30 and LD 100/30 after whole body exposure was estimated as 550 and 1,000 rads respectively. 2. The life span of leukocyte in the circulation after irradiation was slightly shortened in the 100 rad irradiated group, as compared with the unirradiated control group, but markedly shortened in the 300, 550 and 1,000 rads irradiated group. 3. After irradiation, decrease of leukocyte half survival time in the circulation showed the same pattern as that of leukocytes life span. 4. As the irradiation doses increased, the elution rate of $^{51}Cr$ loss from $^{51}Cr$ tagged leukocytes in the circulation were markedly increased gradually. 5. The life span shortening of leukocytes in the circulation after irradiation seems to occure by two processes of senescence acceleration and early destruction.

• The Korean Journal of Physiology
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• 제3권2호
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• pp.17-23
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• 1969

Oxygen consumption rate $(QO_2)$ and protein content of liver and kidney of the Ehrlich ascites tumor-bearing mouse were measured from 6th till 14th day after the inoculation of $4{\times}10^6$ Ehrlich ascites tumor cells. The results thus obtained were compared with those of the groups in which; 1) Whole body x-irradiation with 400 r was done to mouse prior to the inoculation of $4{\times}10^6$ Ehrlich ascites tumor cells, 2) Same number of the irradiated tumor cells were inoculated after subjecting the tumor cells to x-irradiation with 400 r or 900 r in vitro, and 3) the normal, and the following results were obtained; 1. $QO_2$ of the liver and kidney of the tumor-bearing mouse were all lower than the normal and a gradual decrease of $QO_2$ in both liver and kidney was noted as the ascites tumor was progressively developing. 2. In the groups where whole body x-irradiation with 400 r was done, or x-irradiation of ascites tumor cells in vitro with either 400 r or 900 r, $QO_2$ of the liver and kidney were lower than the normal, and the pattern of the decrease was similar in the case of the tumor-bearing mouse. 3. Protein contents in all the groups showed lower values than the normal, and the decrease was gradual as the ascites tumor was developing. 4. $QO_2$ and protein levels in the liver were generally lower than those in the kidney. 5. A certain cancerous metabolism was, therefore, noted in the remote organs of Ehrlich ascites tumor-bearing animal.

• Journal of Microbiology and Biotechnology
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• 제24권1호
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• pp.127-131
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• 2014

While searching for lactic acid bacteria that can restore aging-impaired immune responses, we isolated the Toll-like receptor (TLR) 2/NF-${\kappa}B$-activating strain Lactobacillus plantarum HY7712 from kimchi and investigated its immunomodulating effect in whole-body ${\gamma}$-irradiated mice. Exposure to HY7712 strongly activated NF-${\kappa}B$ signaling in RAW264.7 cells, but inhibited lipopolysaccharide-stimulated NF-${\kappa}B$ activation. Moreover, HY7712 protected against the downregulation of interferon (IFN)-${\gamma}$ and upregulation of interleukin (IL)-13 caused by ${\gamma}$-irradiation in mice. In mice, ${\gamma}$-irradiation impaired NK-cell activity against YAC-1 tumor cells, but following HY7712 exposure, the activity of NK cells was restored to 91.5% of the level measured in control mice (p < 0.05). These findings suggest that HY7712 activates the TLR2/NF-${\kappa}B$ signaling pathway and protects against the impairment of NK-cell activity caused by ${\gamma}$-irradiation or aging.