• Title/Summary/Keyword: White spot

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Spot Heating Technology Development for Strawberry Cultivated in a Greenhouse by Using Hot Water Pipe (온수배관을 이용한 시설딸기 부분난방기술 개발)

  • Moon, Jongpil;Kang, Geum-Choon;Kwon, Jin-Kyung;Paek, Yee;Lee, Tae Seok;Oh, Sung-Sik;Nam, Myeong-Hyeon
    • Journal of The Korean Society of Agricultural Engineers
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    • v.58 no.5
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    • pp.71-79
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    • 2016
  • The effects of spot heating for growing the strawberry cultivated in a plastic greenhouse during the winter that were estimated in Nonsan strawberry experiment station located in Chungnam. The temperature of water for heating was controlled by a electric hot water boiler and kept at the range of $22{\sim}24^{\circ}C$. Heating pipes were set up in root zone for root zone heating and very close to crown for crown heating. Spot heating effects were estimated by applying spot heating system in three test factors of heating root zone, crown only and crown plus root zone. The material for crown heating pipe was white low density polyethylene and the nominal diameter of that pipe was 16 mm. The material for root zone heating pipe was flexible stainless steel and the nominal diameter of that pipe was 15A. The flow rate of heating water circulation was 480 L/h and water circulation lasted for all day long. Temperatures, harvest yield by test beds were surveyed from Nov. 10, 2013 to Apr. 29, 2014. The temperature of crown spot for crown heating bed was at the range of $13.0{\sim}17.0^{\circ}C$ during the night and that of crown spot in control bed was at the range of $8.0{\sim}14.0^{\circ}C$. Also, the temperature of root zone for root zone heating bed was at the range of $18{\sim}21.0^{\circ}C$ and that of root zone in control bed was at the range of $13.0{\sim}15.0^{\circ}C$. The cumulative yield growth rate in earlier harvest period (from Dec. 20 to Mar. 15) of crown heating bed was 43% compared with that of control bed and the cumulative yield of crown plus root zone heating bed was 39 % and that of root zone heating bed was 39 %.

Survey of Major Diseases Occurred on Apple in Northern Gyeongbuk from 2013 to 2014 (2013-2014년도 경북 북부지역 사과 주요 병해 발생조사)

  • Cheon, Wonsu;Jeon, Yongho
    • Research in Plant Disease
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    • v.21 no.4
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    • pp.261-267
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    • 2015
  • During the period from 2013 to 2014, disease occurrences by various pathogens in apple cultivars have been investigated in northern Gyeongbuk province of Korea. Anthracnose, white rot, Alternaria leaf spot, Marssonina blotch, and bacterial shoot blight as major diseases have been observed. Pathogens isolated from the symptomatic plants were identified as Colletotrichum gloeosporioides for anthracnose, Botryosphaeria dothidea for white rot, Alternaria alternata for Alternaria leaf spot, Marssonina mali for Marssonina blotch, and Pseudomonas syringae pv. syringae for bacterial shoot blight. Of all diseases, the bacterial shoot blight has been severely increased in chronically infested fields in Gyeongbuk province.

Cloning and Purification of Envelope Proteins (VP19, VP28) and Nucleocapsid Proteins (VP15, VP35) Genes of a Shrimp White Spot Syndrome Virus Isolates in Korea

  • Seok, Seung-hyeok;Park, Jae-hak
    • Proceedings of the Korean Society of Veterinary Pathology Conference
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    • 2003.10a
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    • pp.41-41
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    • 2003
  • White spot syndrome virus (WSSV) is the causative agent of a disease that has led to severe mortalities of cultured shrimps in Korea and many other countries. Since 1993, massive mortalities due to the viral infection have also occurred in the penaeid shrimps cultured in Korea. WSSV is a large, circular, double stranded (ds) DNA virus and an enveloped, ellipsoid virus with a rod-shaped nucleocapsid with flat ends. In order to identify the characteristics of this Korean isolate of WSSV, the genes for four virion proteins, VP15, VP19, VP28 and VP35 were cloned and their sequences were compared with the available pool of WSSV gene sequences in the GenBank/EMBL databases. From these comparisons, we confirm the occurrence of WSSV in Korea and deduce that, VP15, VP28 and VP35 genes are identically conserved among the Korean isolate and geographically different foreign isolates, but VP19 amino acid sequences of the Korean WSSV isolates changed valine of the foreign isolates into aspartate. (omitted)

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Vaccination of Shrimp (Penaeus chinensis) against White Spot Syndrome Virus (WSSV)

  • Ha, Yu-Mi;Gong, Soo-Jung;Nguyen, Thi-Hoai;Ra, Chae-Hun;Kim, Ki-Hong;Nam, Yoon-Kwon;Kim, Sung-Koo
    • Journal of Microbiology and Biotechnology
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    • v.18 no.5
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    • pp.964-967
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    • 2008
  • Two structural protein genes, VP19 and VP466, of white spot syndrome virus (WSSV) were cloned and expressed in Sf21 insect cells using a baculovirus expression system for the development of injection and oral feeding vaccines against WSSV for shrimps. The cumulative mortalities of the shrimps vaccinated by the injection of rVP19 and rVP466 at 15 days after the challenge with WSSV were 50.2% and 51.8%, respectively. For the vaccination by oral feeding of rVP19 and rVP466, the cumulative mortalities were 49.2% and 89.2%, respectively. These results show that protection against WSSV can be generated in the shrimp, using the viral structural protein as a protein vaccine.

First Report of Leaf Spot Disease Caused by Cladosporium pseudocladosporioides on Morus alba in South Korea

  • Heo, Jung-In;Oh, Ji Yeon;Lee, Dong-Hyeon
    • Journal of Forest and Environmental Science
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    • v.37 no.4
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    • pp.338-340
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    • 2021
  • Morus alba, known as White Mulberry, is one of the most common species of mulberry found in South Korea, along with M. australis, known as Korean Mulberry. During a routine survey to investigate fungal diseases on deciduous broad-leaved trees in 2020, leaf spots were consistently observed on the White Mulberry in Sejong-si (36°30'12.8"N 127°17'34.5"E) and Wonju-si (37°15'29.6"N 128°11'37.9"E), South Korea, with a disease incidence of approximately 70 to 80%. Symptoms included circular, tan or necrotic lesions surrounded by a dark margin on leaves, which, in some cases, the lesions coalesced to form relatively large necrotic regions. The pathogen was successfully isolated from M. alba, and was identified as Cladosporium pseudocladosporioides based on the phylogenetic analysis and morphology. To the best of our knowledge, this is the first report of leaf spot disease on M. alba caused by C. pseudocladosporioides in South Korea.

Real-time PCR Quantification of White Spot Syndrome Virus (WSSV) and Hepatopancreatic Parvovirus (HPV) Loads in Shrimp and Seawaters of Shrimp Ponds on the West Coast of South Korea

  • Jang, In-Kwon;Gopalakannan, Ayyaru;Suriakala, Kannan;Kim, Jong-Sheek;Kim, Bong-Rae;Cho, Yeong-Rok;Meng, Xian-Hong;Seo, Hyeong-Chul
    • Fisheries and Aquatic Sciences
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    • v.11 no.4
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    • pp.195-204
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    • 2008
  • Viral diseases are major emerging problems of shrimp that have affected the production, and even complete losses for shrimp farms. In this study, we developed a sensitive TaqMan real-time PCR method to quantify white spot syndrome virus (WSSV) and hepatopancreatic parvovirus (HPV) in the shrimp and pond water in which fleshy shrimp, Fenneropenaeus chinensis, and Pacific white shrimp, Litopenaeus vannamei, are reared. WSSV and HPV in pond seawaters ranged from $1.65{\times}10^3$ to $2.43{\times}10^9$ and from 0 to $4.43{\times}10^5$ copies/L of seawater, respectively. Of 20 ponds analyzed, all pond water and shrimp were positive for WSSv. L. vannamei showed higher susceptibility to WSSV than F chinensis. HPV was detected only in the pond water for F chinensis. In shrimp tissue, however, HPV was found in both species, with 23-times higher infection rate in F chinensis than L. vannamei. The total bacterial counts in the pond water ranged from $2.23{\times}l0^3$ to $1.98{\times}l0^5\;CFU/mL$. The variations in total bacterial count for each pond appeared to correlate to the variations of the WSSV load. Statistical analysis indicated that there was no significant difference (P>0.05) between the WSSV load in pond water and shrimp, and there was no relationship between total bacterial load and viral load in the pond water. However, a significant difference (P<0.01) was found between HPV load and L. vannamei and F chinensis pond water.

Mechanism of Crack Formation in Pulse Nd:YAG Laser Spot Welding of Al Alloys (Al합금 펄스 Nd:YAG 레이저 점 용접부의 균열 발생기구)

  • 하용수;조창현;강정윤;김종도;박화순
    • Journal of Welding and Joining
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    • v.18 no.2
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    • pp.86-94
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    • 2000
  • This study was performed to investigate types and formation mechanism of cracks in two Al alloy welds, A5083 and A7N01 spot-welded by pulse Nd : YAG laser, using SEM, EPMA and Micro-XRD. In the weld zone, three types of crack were observed : center line crack({TEX}$C_{C}${/TEX}), diagonal crack({TEX}$C_{D}${/TEX}), and U shape crack({TEX}$C_{U}${/TEX}). Also, HAZ crack({TEX}$C_{H}${/TEX}) was observed in the HAZ region, furthermore, mixing crack({TEX}$C_{M}${/TEX}) consisting of diagonal crack and HAZ crack was observed. White film was formed at th hot crack region in the fractured surface after it was immersed to 10% NaOH water. In the case of A5083 alloy, white films in {TEX}$C_{C}${/TEX} crack and {TEX}$C_{D}${/TEX} crack region were composed of low melting phases, {TEX}$Fe_{2}SiAl_{8}${/TEX} and eutectic phases, $Mg_2$Al$_3$ and $Mg_2$Si. Such films observed $CuAl_2$, {TEX}$Mg_{32}(Al,Zn)_{3}${/TEX}, MgZn$_2$, $Al_2$CuMg and $Mg_2$Si were observed in the whitely etched films near {TEX}$C_{C}${/TEX} crack and {TEX}$C_{D}${/TEX} crack regions. The formation of liquid films was due to the segregation of Mg, Si, Fe in the case of A5083 alloy and Zn, Mg, Cu, Sim in the case of A7N01 alloy, respectively. The {TEX}$C_{C}${/TEX} and {TEX}$C_{D}${/TEX} cracks were regarded as a result of the occurrence of tensile strain during the welding process. The formation of {TEX}$C_{M}${/TEX} crack is likely to be due to the presence of liquid film at the grain boundary near the fusion line in the base metal as well as in the weld fusion zone during solidification. The {TEX}$C_{U}${/TEX} crack is considered a result of the collapsed keyhole through incomplete closure during rapid solidification.

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Weld Quality Evaluation Method for the Resistance Spot Welds using X-ray Transmission Inspection (X-선 투과검사를 이용한 저항 점용접부 품질평가기법)

  • Lee, Jong-Dae;Lee, So-Jeong;Bang, Jung-Hwan;Yoon, Gil-Sang;Kim, Mok-Soon;Kim, Jun-Ki
    • Journal of Welding and Joining
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    • v.32 no.6
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    • pp.1-7
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    • 2014
  • For the resistance spot welds of CR1180 and GA1180 TRIP steels, the weld quality evaluation method using the digitalized X-ray transmission imaging apparatus was investigated in comparison with the crosssectional examination method. In the case of the resistance spot welding of CR1180, three circular regions, such as WZ(white zone), GZ(grey zone) and DZ(dark zone), appeared on X-ray image and they corresponded to the diameters of indentation mark, nugget and corona bond, respectively. The variation of X-ray transmission thickness due to the thickness variation of the weld seemed to be mainly responsible for the formation of those contrasts. The X-ray image contrast formed from the variation of transmission thickness at the outer border line of DZ could also enable the inspections of the notch shape, nonuniformity of the welding pressure and spatter from its sharpness, concentricity and the normal straight line, respectively. The X-ray image of the resistance spot weld of galvannealed GA1180 TRIP steel was very similar to that of CR1180 TRIP steel except the crown shaped outer border line of DZ which was considered to be due to the melting behavior of zinc having the boiling temperature even lower than the melting temperature of steel.

Black Leaf Spot of Dendrobium phalaenopsis Caused by Fusarium moniliforme (Fusarium moniliforme에 의한 덴파레 검은점무늬병)

  • Lee, Dong-Hyun;Hur, Jae-Seoun;Koh, Young-Jin
    • Research in Plant Disease
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    • v.9 no.3
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    • pp.149-152
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    • 2003
  • Black leaf spot disease occurred on Dendrobium phalaenopsis grown in farmer's fields located in Bonggangmyon, Gwangyang-eup, Jeonnam, Korea. Black small spots occurred on leaves at initial stage of infection and the infected leaves turned yellow from the tip. The yellow leaves were falled, resulting in stem blighting or eventual death of the entire plant. White mycelial colony of the causal fungus grown on potato dextrose agarturned dark violet later and optimum temperature for the mycelial growth was $25^{\circ}C$. The causal fungus isolated from the black leaf spot on D. phalaenopsis was identified as Fusarium moniliforme based on the mycological characteristics and pathogenicity. The fungus also caused same symptoms on leaves of Phalaenopsis sp. and Cymbidium sp. as well sa D. phalaenopsis by wound inoculation. This is the first report on black leaf spot of D. phalaenopsis caused by F.moniliforme in Korea.

Retrieval Spectral Albedo using red and NIR band of SPOT/VGT

  • Lee, Chang Suk;Seo, Min Ji;Han, Kyung-Soo
    • Korean Journal of Remote Sensing
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    • v.30 no.3
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    • pp.367-373
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    • 2014
  • Albedo is one of the critical parameters for understanding global climate change and energy/water balance. In this study, we used red and NIR reflectance from Satellite Pour I'Obervation de la Terre (SPOT)/Vegetation (VGT) S1 product. The product is preprocessed for users that they are atmospherically corrected using Simple Method Atmospheric Correction (SMAC) by Vision on Technology (VITO) for calculating broadband albedo. Roujean's Bi-directional Reflectance Distribution Function (BRDF) model is a semi-empirical method used for BRDF angular integration and inversion. Each kernel of Roujean's model was multi integrated by angle components (i.e., viewing zenith, solar zenith, and relative azimuth angle). Black-sky hemispherical function is integrated by observational angle; whereas, white-sky hemispherical efficient is integrated by incident angle. Estimated spectral albedo of red ($0.61{\sim}0.68{\mu}m$, B2) and near infrared ($0.79{\sim}0.89{\mu}m$, B3) have a good agreement with MODIS albedo products.