• The Plant Pathology Journal
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• v.19 no.4
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• pp.189-194
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• 2003

The effects of prochloraz on membrane permeability and germination of uredospores of Puccinia recondita were investigated to determine its potential mode of action on wheat leaf rust control activity. Disease control activity of ergosterol biosynthesis inhibitors (EBIs) and their activities on uredospore membrane permeability and germination were examined with wheat leaf rust pathogen, both in vitro and in vivo. While wheat leaf rust was not controlled by prochloraz, electrolytic leakage and spore germination of P. recondita uredospore was the highest with the use of prochloraz among the eight fungicides tested. Prochloraz stimulated uredospore of P. recondita to germinate at a higher ratio. Although certain EBIs, such as hexaconazole, showed excellent control activity, their effects on uredospore membrane permeability and germination was much inferior to prochloraz. Therefore, results of this study suggest that effects of EBIs on membrane permeability and germination of uredospore are not always correlated with their disease control activity.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.3
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• pp.386-393
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• 2011

Three trials were conducted to determine the available energy of different wheat screening varieties collected from different locations of Khorasan in Iran. In experiment 1, chemical composition and the nitrogen corrected true metabolisable energy (TMEn) were evaluated. A precision-fed rooster assay was used, in which, each wheat screening sample was tube fed to adult roosters, and the excreta were collected for 48-h. In Exp. 2 and 3, five and two wheat screening verities-based diets with or without xylanase and phytase were fed to 16-day old battery reared chicks respectively, and total feed consumption and excreta were measured during next three days. The variable nature of wheat screening varieties led to significant differences in mean TMEn values (p<0.01). The TMEn values of samples determined with adult roosters varied by ${\pm}5.03%$ of the mean value ($3,097.65{\pm}49.32\;kcal/kg$) and ranged from 2,734.90 to 3,245.12 kcal/kg. There was a significant correlation (p<0.05) between crude fiber (CF), neutral detergent fiber (NDF), and acid detergent fiber (ADF) with TMEn, and the greatest correlation coefficient was observed between NDF and TMEn (r = -0.947; p<0.001). The optimal equation in terms of $R^2$ from using a single chemical analysis was obtained with NDF: TMEn = 4,152.09-27.80 NDF ($R^2$ = 0.90, p<0.0001), and the TME prediction equation was improved by the addition of the crude protein (CP) and ASH content to sequential analysis: TMEn = 3,656.97-28.65 NDF+32.54 CP+38.70 ASH ($R^2$ = 0.98, p<0.0001). The average AMEn values of 5 and 2 wheat screening varieties determined with young broiler chickens were $2,968.41{\pm}25.70\;kcal/kg$ and $2,976.38{\pm}8.34\;kcal/kg$ in Exp. 2 and Exp. 3, respectively. Addition of xylanase and phytase to wheat screenings resulted in significant (p<0.01) improvement in AMEn by 4.21 and 2.92%, respectively.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.1
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• pp.78-85
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• 1999

To investigate kernel hardness, a compression test which is widely used to measure the hardness of individual kernels as a physical testing method was made simultaneously with the measurement of friabilin (15KDa) which is strongly associated with kernel hardness and was recently developed as a biochemical marker for evaluating kernel hardness in 79 Korean wheat varieties and experimental lines. With the scattered diagram based on the principal component analysis from the parameters of the compression test, 79 Korean wheat varieties were classified into three groups based on the principal component analysis. Since conventional methods required large amount of flour samples for analysis of friabilin due to the relatively small amount of friabilin in wheat kernels, those methods had limitations for quality prediction in wheat breeding programs. An extraction of friabilin from the starch of a single kernel through cesium chloride gradient centrifugation was successful in this experiment. Among 79 Korean wheat varieties and experimental lines 50 lines (63.3%) exhibited a friabilin band and 29 lines (36.7%) did not show a friabilin band. In this study, lines that contained high maximum force and the lower ratio of minimum force to maximum force showed the absence of the friabilin band. Identification of friabilin, which is the product of a major gene, could be applied in the screening procedures of kernel hardness. The single kernel analysis system for friabilin was found to be an easy, simple and effective screening method for early generation materials in a wheat breeding program for quality improvement.

• The Plant Pathology Journal
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• v.36 no.6
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• pp.558-569
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• 2020

Fusarium head blight (FHB) is a devastating fungal disease of wheat (Triticum aestivum L.). The lack of genetic resources with stable FHB resistance combined with a reliable and rapid screening method to evaluate FHB resistance is a major limitation to the development of FHB resistant wheat germplasm. The present study utilized an immature wheat spike culture method to screen wheat spike culture derived variants (SCDV) for FHB resistance. Mycotoxin concentrations determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS) correlated significantly (P < 0.01) with FHB severity and disease progression during in vitro spike culture. Selected SCDV lines assessed for FHB resistance in a Fusarium field disease nursery in Carman, Manitoba, Canada in 2016 showed significant (P < 0.01) correlation of disease severity to the in vitro spike culture screening method. Selected resistant SCDV lines were also crossed with an elite cv. CDC Hughes and the progeny of F₂ and BC₁F₂ were screened by high resolution melt curve (HRM) analyses for the wheat UDP-glucosyl transferase gene (TaUGT-3B) single nucleotide polymorphism to identify resistant (T-allele) and susceptible (G-allele) markers. The progeny from the crosses were also screened for FHB severity using the immature spike culture method and identified resistant progeny grouped according to the HRM genotyping data. The results demonstrate a reliable approach using the immature spike culture to screen for FHB resistance in progeny of crosses in early stage of breeding programs.

• Journal of Microbiology and Biotechnology
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• v.14 no.2
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• pp.422-425
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• 2004

To discover a potent strain against wheat leaf rust, soil samples collected from Ilgamho, Seoul, Korea were tested in vivo and a strain belonging to Streptomyces sp. was found to show good antifungal activity when fermented in a broth. The identification of the strain was carried out based on 16S rDNA analysis, and the active compound was separated from the fermented broth. Even though its structure was not determined completely, the authors report the results obtained so far indicate that the fermented broth of the strain showed activity against wheat leaf rust. Therefore, we propose that this may be a potential novel strain showing antifangal activity against Puccinia recondita.

• Korean Journal of Microbiology
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• v.19 no.3
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• pp.108-114
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• 1981

In order to isolate microorganisms which produce antibiotics aganist wheat black rust, some bacteria, molds, and actinomycetes were isolated from soils and screened for the production of antibiptics against wheat black rust. Beacuse wheat black rust-puccinia graminis--is a complete parsitic mold which can't grow in artifical medium, new method for the screening of antibiotic producing microorgsnisms against wheat black rust developed by using live leaves of wheat. With new method, a strain No. $480HS_{20}$ which produces a substnace having strong and Puccinia graminis activity and very narrow antimicrobial spectrum was isolated. the substance produced by the strain No.$480HS_{20}$ had better anti Puccinia graminis activity than any other known antifungal antibotics such as kasurgamycin, balasticidins, actidione, antimycin, ologomycin. And the substance was observed to be very stable at heat and ultraviolet light. The strain was indentified as Bacillus subtilis.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.300-300
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• 2022

Wheat (Triticum spp.) is an important source of food worldwide and the focus of considerable efforts to identify new combinations of genetic diversity for crop improvement. In particular, wheat starch composition is a major target for changes that could benefit human health. Starches with increased levels of amylose are of interest because of the correlation between high amylose content and elevated levels of resistant starch, which has been shown to have beneficial effects on health for combating obesity and diabetes. In this study, high amylose wheat germplasms from other countries were collected and cultivated in Korea, and then the content of amylose was evaluated, we examined amylose content in 614 wheat germplasm. Furthermore, amylose content was validated using several milling processes such as roller, hammer, and grinding mill. As a result, the amylose content distribution was divided into five groups. The range of the amylose levels in whole wheat flour was 18.3% to 29.6%. In addition, the mutant lines were screened for high amylose, and two mutant lines (WX-1046 and WX-1074) exhibited a comparable amylose content to Keumkang whole wheat (19.6%). It has been established that high amylose indicated SS IIa null and necessitate GBSS. Based on these findings, it may be helpful to develop high amylose wheat germplasm and production techniques, particularly in Korea.

• Journal of Microbiology and Biotechnology
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• v.16 no.3
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• pp.465-468
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• 2006

Even though many pesticides are known for barley powdery mildew and wheat leaf rust, alternative controls are necessary, because of consumer rejection of chemical pesticides and the appearance of fungi resistant to fungicides. To discover biopesticides, many broths of microorganisms were screened. Of those, a culture broth of Burkholderia ambifaria showed an excellent antifungal activity against both Erysiphe graminis and Puccinia recondita, which cause barley powdery mildew and wheat leaf rust, respectively.

• The Korean Journal of Pesticide Science
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• v.9 no.2
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• pp.122-131
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• 2005

For development of new agrochemical fungicide, syntheses of pyrrolo[2,1-b]thiazole carboxanilide derivatives 9 and antifungal screening against 6 kinds of plant pathogens were carried out. Functionalization of carboxylic acid on C-2 into carboxanilide in the pyrrolo[2,1-b]thiazole resulted in new 20 candidates, in which are ${\alpha},{\beta}$-unsaturated carboxanilide and methyl groups that are in cis relationship. Treatment of acetoxy-1,4-thiazin with magnesium in refluxing methanol gave pyrrolo[2,1-b]thiazole ethyl ester 4 in higher yield than that of the previous report. Hydrolysis of this compound afforded the corresponding acid 5, which reacted with aniline derivatives in the presence of coupling reagent, DIC to give pyrrolo[2,1-b]thiazole carboxanilides 9. As the result of in vivo antifungal assay of 9 against rice blast, rice sheath blight, cucumber gray mold, tomato late blight, wheat leaf rust, and barley powdery mildew, some compounds showed selectively antifungal activities against the rice blast and wheat leaf rust.

• Proceedings of the Korean Society of Crop Science Conference
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• 2020.11a
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• pp.111-111
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• 2020