• Journal of Periodontal and Implant Science
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• v.49 no.5
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• pp.270-286
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• 2019

Purpose: Despite the well-known anti-inflammatory effects of vitamin D in periodontal health, its mechanism has not been fully elucidated. In the present study, the effect of vitamin D on strengthening E-cadherin junctions (ECJs) was explored in human gingival keratinocytes (HGKs). ECJs are the major type of intercellular junction within the junctional epithelium, where loose intercellular junctions develop and microbial invasion primarily occurs. Methods: HOK-16B cells, an immortalized normal human gingival cell line, were used for the study. To mimic the inflammatory environment, cells were treated with tumor necrosis factor-alpha ($TNF-{\alpha}$). Matrix metalloproteinases (MMPs) in the culture medium were assessed by an MMP antibody microarray and gelatin zymography. The expression of various molecules was investigated using western blotting. The extent of ECJ development was evaluated by comparing the average relative extent of the ECJs around the periphery of each cell after immunocytochemical E-cadherin staining. Vitamin D receptor (VDR) expression was examined via immunohistochemical analysis. Results: $TNF-{\alpha}$ downregulated the development of the ECJs of the HGKs. Dissociation of the ECJs by $TNF-{\alpha}$ was accompanied by the upregulation of MMP-9 production and suppressed by a specific MMP-9 inhibitor, Bay 11-7082. Exogenous MMP-9 decreased the development of ECJs. Vitamin D reduced the production of MMP-9 and attenuated the breakdown of ECJs in the HGKs treated with $TNF-{\alpha}$. In addition, vitamin D downregulated $TNF-{\alpha}$-induced nuclear factor kappa B ($NF-{\kappa}B$) signaling in the HGKs. VDR was expressed in the gingival epithelium, including the junctional epithelium. Conclusions: These results suggest that vitamin D may avert $TNF-{\alpha}$-induced downregulation of the development of ECJs in HGKs by decreasing the production of MMP-9, which was upregulated by $TNF-{\alpha}$. Vitamin D may reinforce ECJs by downregulating $NF-{\kappa}B$ signaling, which is upregulated by $TNF-{\alpha}$. Strengthening the epithelial barrier may be a way for vitamin D to protect the periodontium from bacterial invasion.

• The Plant Pathology Journal
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• v.31 no.2
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• pp.165-175
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• 2015

Anthracnose is a fungal disease caused by Colletotrichum species that is detrimental to numerous plant species. Anthracnose control with fungicides has both human health and environmental safety implications. Despite increasing public concerns, fungicide use will continue in the absence of viable alternatives. There have been relatively less efforts to search antagonistic bacteria from mudflats harboring microbial diversity. A total of 420 bacterial strains were isolated from mudflats near the western sea of South Korea. Five bacterial strains, LB01, LB14, HM03, HM17, and LB15, were characterized as having antifungal properties in the presence of C. acutatum and C. gloeosporioides. The three Bacillus atrophaeus strains, LB14, HM03, and HM17, produced large quantities of chitinase and protease enzymes, whereas the B. amyloliquefaciens strain LB01 produced protease and cellulase enzymes. Two important antagonistic traits, siderophore production and solubilization of insoluble phosphate, were observed in the three B. atrophaeus strains. Analyses of disease suppression revealed that LB14 was most effective for suppressing the incidence of anthracnose symptoms on pepper fruits. LB14 produced antagonistic compounds and suppressed conidial germination of C. acutatum and C. gloeosporioides. The results from the present study will provide a basis for developing a reliable alternative to fungicides for anthracnose control.

• Journal of Industrial Convergence
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• v.21 no.2
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• pp.103-109
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• 2023

In Korea, software education is implemented from elementary school. As a representative software education tool for elementary schools, various chess games reconstructed based on the rules of Western chess games are being used. On the other hand, Yutnori, one of our traditional games, also includes elements of software education, so research on this is needed. Therefore, in this study, a Yutnori program based on computational thinking using an educational programming language, Entry, and a turtle robot was developed and its validity verified. As a result of the validity verification, the CVR value was higher than 0.7 in the degree of agreement with the subject achievement standard (3 questions), the appropriateness of learning materials (4 questions), and the possibility of class application (3 questions). Therefore, it could be judged that the learning program developed in this study has a high level of agreement with the subject achievement standards, appropriate learning materials, and high possibility of being applied to classes. In order to generalize this content in the future, the effectiveness will need to be verified, and experimental research will be needed to understand this.

• The Korea Journal of Herbology
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• v.31 no.5
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• pp.41-46
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• 2016

Objectives : The Skin is composed of multiple layers, including the epidermis, dermis, and hypodermis. It provides a vital barrier structure that protects vertebrates from external environmental antigens, solvents, ultraviolet light, microorganisms, toxins, and weather conditions. Although several biological effects of Mori Follium have been reported, beneficial effects of Mori Follium in skin health remain unclear. In this study, we prepared water extract of Mori Follium (MLE) and evaluated the effects on melanin accumulation and expression levels of skin fibril-related proteins.Methods : The cytotoxicities of MLE in B16F10 melanoma and human skin fibroblasts (HSF) were examined by MTT assay. Inhibitory effect of MLE on the α-MSH- and IBMX-induced melanosis in B16F10 melanoma was examined. The expression levels of fibronectin, collagen 1α2, and CCN2 in MLE-treated HSF were analyzed by reverse transcription-polymer chain reaction (RT-PCR) and western blotting.Results : The MLE treatment for 24 h did not affect to the B16F10 and HSF at concentrations of 1, 10, 50, 100, 200, 400 and 800 ㎍/ml. The MLE treatment for 72 h significantly and dose dependently suppressed melanin accumulation in B16F10 melanoma. In addition, the MLE treatment up-regulated expression levels of skin fibril-related genes such as fibronectin, collagen 1α2, and CCN2 in HSF. Our western blot analysis revealed MLE-induced up-regulation of skin fibril-related genes required the activation of CCN2 protein.Conclusions : In conclusion, these findings suggest that the MLE could be used in development of cosmetic natural material of maintaining healthy skin.

• The Korean Journal of Physiology and Pharmacology
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• v.21 no.4
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• pp.361-370
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• 2017

Previous reports have suggested that physical and psychological stresses may trigger fibromyalgia (FM). Stress is an important risk factor in the development of depression and memory impairments. Antidepressants have been used to prevent stress-induced abnormal pain sensation. Among various antidepressants, tianeptine has been reported to be able to prevent neurodegeneration due to chronic stress and reverse decreases in hippocampal volume. To assess the possible effect of tianeptine on FM symptoms, we constructed a FM animal model induced by restraint stress with intermittent cold stress. All mice underwent nociceptive assays using electronic von Frey anesthesiometer and Hargreaves equipment. To assess the relationship between tianeptine and expression levels of brain-derived neurotrophic factor (BDNF), cAMP response element-binding protein (CREB), and phosphorylated cAMP response element-binding protein (p-CREB), western blotting and immunohistochemistry analyses were performed. In behavioral analysis, nociception tests showed that pain threshold was significantly decreased in the FM group compared to that in the control group. Western blot and immunohistochemical analyses of medial prefrontal cortex (mPFC) and hippocampus showed downregulation of BDNF and p-CREB proteins in the FM group compared to the control group. However, tianeptine recovered these changes in behavioral tests and protein level. Therefore, this FM animal model might be useful for investigating mechanisms linking BDNF-CREB pathway and pain. Our results suggest that tianeptine might potentially have therapeutic efficacy for FM.

• Proceedings of the Korean Institute of Resources Recycling Conference
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• 2004.12a
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• pp.35-42
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• 2004

In spite of economic slump of global IT market, China is showing growth of $25\%$ per a year. After opening economy, the economic growth of the country haven't flagged. Moreover, According to the economic plan of the President HuJinTao who took office in 2002, IT field has been developing quickly. Even though such development is enough to show off the ability of China in the world, it is also a fart that there are many side effects. Among them, the imbalance of development between the east and the west is disturbing the general Chinese development. So the government is making efforts to reduce the gap as a solution, 'Go West campaign.' While progressing the policy, the related method which restricted domestic demand market advance from 2000 is abolished. We also established a bridgehead for lauching to the west of China at the same line with other developed countries. Based ell such reality, we understands the general present state and policy of Chinese IT industrial and the opportunity for abolishing the related method which restricted domestic market advance to also obtain from 2000, and for our country to also advance to the western area in China by the start onboard equal to other advanced nations. The direction to which we have to go by investigating the situation of a western area is investigated.

• Korean journal of food and cookery science
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• v.29 no.4
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• pp.343-351
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• 2013

To help reduce the 'breakfast-skip' rate of the high school students and improve such 'breakfast-skipping' practice this study devised a new covenience menu for school breakfast Program. Dictated by a dietitian the form of cooking was rendered within directly-cookable convenient food; The price of menu ranged from 1,500-2,000won taking into account the parents' survey ; the menu formation drew largely from the student food preference survey and their favorites. The composition of menu - the Korean dish and the western dish put together-were of two folds and was based on the two: main menu, subsidiary menu. While main menu used rice, the subsidiary one used fruits, salads, milk, and juices. Energy intake sufficiency standard was set at a level of between 1/4 and 1/3 of the relevent group(mid&high students, 15-18 yrs old)'s approximated energy need, drawn from the Dietary Reference Intakes For Koreans(2010). 10 disparate, divised menus were tested deliberately- cooked at a site, thereafter qualifed and supplemented to meet the targeted energy amount. The nuturitional facts of each menu set were measured through the usage of CAN-PRO 3.0, Computer Aided Nutritional Analysis Program, for professionals. Completed menu set were then given in photograph. The results of this study were as follows : The results of this study were as follows : the newly developed menu's calories and nuturitional content were as follows in the ratio of Dietary Reference Intakes For Koreans(2010) : Calories 95.7%(4.3% below the targeted intake standard), Protein(131.1%), Calcium(130.2%) the both of which marked higher than the recommended daily intake, iron(71.0%), Potassium(93.1%) both of which figure lower than the recommended daily intake. The cost of ingredients per each person was 1,335won, which was lower than the targeted cost(1500won). When calculated, hypothetically provided breakfast's labor hour needed per 1 meal is shown to be average 1.2 minute. In conslusion : Convenience style school breakfast program would bring an increse in the intake of high school students' breakfast, which may contribute not only to the sound physical growth but to academic performance. Various developments of the school breakfast program is much needed continually.

• Journal of the Korean Professional Engineers Association
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• v.5 no.17
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• pp.3-10
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• 1972

This report is the result of the basic geologic investigation for the purpose of preparing the long-term development program of the U-jeon Consolidated Coal kline. The Consolidated Coal Mine is located at Gujeol-ri, Wangsan-myeon, Myeongju-gun, and Yucheon-ri, Bug-myeon, Jeongseon-gun, Gan-gweon Province (128$^{\circ}$ 43′10.4"-128$^{\circ}$ 46′10. 4"of east long-ititude, 37$^{\circ}$ 30′-37$^{\circ}$ 33′ of north latitude). This region, the western part of Taebaek mountain range, shows a ragged mountinous feature. Formations of the Pyeongan System of Paleozoic Era are distribu ted in the region with the surrounding Great Limestone Series of Joseon System which covers the south-eastern part of the region. The Pyeongan System is divided into four formations, namely, the Hongjeom, the Sadong, the Gobang and the Hongam, in ascending order. The sadong Formation intercalates several coal beds, and two coal beds out of them are minable. The coal beds are variable in thickness, having the repeated swelled or poket and the pinched parts, which suggest all intense disturbance caused by folding. The heat value of the coal is 5, 500cal. on the average. The total amount of coal reserves of the U-jeon consolidated Coal Mine is estimated at about thirty million metric tons.

• Journal of the Korean Society for information Management
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• v.25 no.4
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• pp.269-281
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• 2008

This paper evaluated the collection development of the Korean collection at the A university over the several decade. Compared against the ACRL standard Formula A, the result demonstrated that the Korean library collection is still needed to increase Korean collection to support Korean-related program and Korean community in the area. When we share western language materials in the library as the same area studies resources, we can evaluate Korean studies collection as Level B. Comparison with similar institutions (ARL comparisons) shows that the collection is the middle among 15 universities which provide Korean programs. The situation for Korean resources at the A university is needed to get more support from Korean institutes and Korean societies to keep up with the Korean collection for academics and regional community users as well.

• Fisheries and Aquatic Sciences
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• v.1 no.2
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• pp.276-282
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• 1998

As a part of the long-term ADCP mooring program to measure the mass flux through the Korea Strait, current velocity data were obtained for 39 days in the deepest point of the strait. Near-surface velocity of this observation was compared with Izuhara-Pusan sea level difference (SLD) to investigate the geostrophic relationship. Principal direction of the Tsushima Current at the mooring station is 44.6 degrees to the north from the east. Variability of the tidal current is greater than the nontidal current by a factor of two. Correlation coefficient of tidal current against SLD is 0.46 but the nontidal current is not correlated. The current velocity (U in cm/s) can be estimated from the demeaned SLD (in cm) by the relation U=23.63+0.64SLD where the maximum range of SLD is 52.9 cm. Current is coherent with SLD at semidiurnal, diurnal and 42.7-hour periods. A dominant nontidal variability with about 5-day period is not coherent with SLD.