• Journal of the Korea Society of Computer and Information
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• v.17 no.7
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• pp.45-52
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• 2012

In this paper, an crowd density in elevator estimation method based on multi-class Adaboost classifier is proposed. The SOM (Self-Organizing Map) based conventional methods have shown insufficient performance in practical scenarios and have weakness for low reproducibility. The proposed method estimates the crowd density using multi-class Adaboost classifier with texture features, namely, GLDM(Grey-Level Dependency Matrix) or GGDM(Grey-Gradient Dependency Matrix). In order to classify into multi-label, weak classifier which have better performance is generated by modifying a weight update equation of general Adaboost algorithm. The crowd density is classified into four categories depending on the number of persons in the crowd, which can be 0 person, 1-2 people, 3-4 people, and 5 or more people. The experimental results under indoor environment show the proposed method improves detection rate by about 20% compared to that of the conventional method.

• Journal of Microbiology and Biotechnology
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• v.13 no.4
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• pp.509-516
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• 2003

A model system for the immnunochemical detection of Escherichia coli O157:H7 using a combined immunomagnetic separation (IMS) and test-strip liposome immunoassay (LIA) procedure was developed. Immunomagnetic beads coated with anti-E. coli O157 IgG antibodies were used to separate the E. coli O157 (including the H7 serotype) from culture. Immunoliposomes, whose surface was conjugated to goat anti-E. coli O157:H7 IgG and which encapsulated the marker dye, sulforhodamine B, were used as a detection label. The test strip, onto which antibodies to goat IgG were immobilized, was the immunosensor capturing immunoliposomes that did not bind to E. coli O157:H7 on the immunomagnetic bead-E. coli O157:H7 complexes. In experiments, pure cell culture suspensions of $10^5 E.$ coli O157:H7 organisms per ml produced a measurable signal inhibition, whereas a weak yet detectable signal inhibition occurred with $10^3CFU/ml$. The inhibition signals increased, when the incubation time for IMS was extended to 90 min and higher IgG-tag density (0.4mol%) was used on the liposomes. With 0.2 and 0.4mol% IgG-tagged liposomes, the IMS-LIA procedure showed more improved signal inhibitions than those of a direct (no IMS) LIA. The combined assay, which measures the instantaneous signal from immunoliposomes, can be completed within 90 min, making it significantly faster than conventional plating methods and enzyme-linked immunosorbent assay (ELISA). Accordingly, it is quite feasible to use the combined immunoassay format of IMS and dye-loaded immunoliposomes for the detection of E. coli O157:H7.