• Journal of Pharmaceutical Investigation
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• v.36 no.1
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• pp.45-51
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• 2006

A rapid, selective and sensitive reversed-phase HPLC method for the determination of dipyridamole in human serum was developed, validated, and applied to the pharmacokinetic study of dipyridamole. Dipyridamole and internal standard, loxapine, were extracted from human serum by liquid-liquid extraction with diethyl ether and analyzed on a Nova Pak $C_{I8}$ column with the mobile phase of 40 mM ammonium acetate:methanol:acetonitrile (35:35:30)(v/v/v, pH 7.8). Detection wavelength of 280 nm and flow rate of 1.0 mL/min were fixed for the study. The assay robustness for the changes of mobile phase pH, organic solvent content, and flow rate was confirmed by $3^3$ factorial design using a fixed dipyridamole concentration (50 ng/mL) with respect to its peak area and retention time. And also, the ruggedness of this method was investigated at three different laboratories using same quality control (QC) samples. This method showed linear response over the concentration range of 2-2000 ng/mL with correlation coefficients greater than 0.999. The lower limit of quantification using 0.5 mL of serum was 2 ng/mL, which was sensitive enough for pharmacokinetic studies of dipyridamole. The overall accuracy of the quality control samples ranged from 103.94 to 105.86% for dipyridamole with overall precision (% C.V.) being 4.60-11.49%. The relative mean recovery of dipyridamole for human serum was 97.64%. Stability studies showed that dipyridamole was stable during storage, or during the assay procedure in human serum. The peak area and retention time of dipyridamole were not significantly affected by the changes of mobile phase pH, organic solvent content, and flow rate under the conditions studied. This method showed good ruggedness (within 15% C.V.) and was successfully used for the analysis of dipyridamole in human serum samples for the pharmacokinetic studies of orally administered Dimor tablet (75 mg as dipyridamole) at three different laboratories, demonstrating the suitability of the method.

• Journal of Pharmaceutical Investigation
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• v.36 no.1
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• pp.23-29
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• 2006

A rapid, selective and sensitive reversed-phase HPLC method for the determination of promethazine in human serum was developed, validated, and applied to the pharmacokinetic study of promethazine. Promethazine and internal standard, chlorpromazine, were extracted from human serum by liquid-liquid extraction with n-hexane containing 0.8% isopropanol and analyzed on a Capcell Pak CN column with the mobile phase of acetonitrile-0.2 M potassium dihydrogen phosphate (42:58, v/v, adjusted to pH 6.0 with 1 M NaOH). Detection wavelength of 251 nm and flow rate of 0.9 mL/min were fixed for the study. The assay robustness for the changes of mobile phase pH, organic solvent content, and flow rate was confirmed by $3^{3}$ factorial design using a fixed promethazine concentration (10 ng/mL) with respect to its peak area and retention time. In addition, the ruggedness of this method was investigated at three different laboratories using same quality control (QC) samples. This method showed linear response over the concentration range of 1-40 ng/mL with correlation coefficients greater than 0.999. The lower limit of quantification using 1 mL of serum was 1 ng/mL, which was sensitive enough for pharmacokinetic studies. The overall accuracy of the quality control samples ranged from 96.15 to 105.40% for promethazine with overall precision (% C.V.) being 6.70-11.22%. The relative mean recovery of promethazine for human serum was 63.54%. Stability (freeze-thaw and short-term) studies showed that promethazine was stable during storage, or during the assay procedure in human serum. However, the storage at $-80^{\circ}C$ for 4 weeks showed that promethazine was not stable. Extracted serum sample and stock solution were not allowed to stand at ambient temperature for 12 hr prior to injection. The peak area and retention time of promethazine were not significantly affected by the changes of mobile phase pH, organic solvent content, and flow rate under the conditions studied. This method showed good ruggedness (within 15% C.V.) and was successfully used for the analysis of promethazine in human serum samples for the pharmacokinetic studies of orally administered Himazin tablet (25 mg as promethazine hydrochloride) at three different laboratories, demonstrating the suitability of the method.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.6
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• pp.442-448
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• 2013

There are two approaches in measuring colors (or chromaticity) in water, i.e., visual comparison method and spectrophotometric method. The color of sample was determined by comparing with that of platinum-cobalt standard solution in the visual comparison method. Single or multiple wavelengths are used for the spectrophotometric method. As the accuracy and precision of visual comparison method depend on the eye sight of the analyzer, the results are not so reliable and representative. In addition, it is hard to measure chromaticity less than 5 TCU. Single wavelength approach in spectrophotometric method, can be applied for groundwater or surface water with natural organic matter (i.e., humic substances) while it's hard to measure the color of wastewater which includes anthropogenic chemical compounds. The measurements with multiple wavelengths approach resulted in reliable data regardless of the source of sample water, i.e., surface water and wastewater. As dozens of measurements and complicated calculations for one sample were required for the multiple wavelengths approach, the approach could not be applied for field measurement. In the present study, the authors tested efficient method which could measure the color of water sample accurately and precisely regardless of the source of water. With the colorimeter with multiple wavelengths and calculation program, the colors of water samples could be measured within 3~4 seconds with accuracy and precision.

• Journal of Sensor Science and Technology
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• v.11 no.4
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• pp.209-217
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• 2002

The developing of buried fiber optic sensor with high sensitivity and broad-area detecting intruders is carried out using fiber optic ROTDR(Rayleigh Optical Time Domain Reflectometry). The sensing part was designed to be able to broad-area detect intrusion effect per optical fiber length under ground. The bending light losses in optical fibers are investigated by commercial mini ROTDR with wavelength $1.55{\mu}m$, distance range 5km, pulse width 20ns, SNR=5.7. The sensing fibers are selected as the common telecommunication fibers are the 1.5mm, 3.5 mm outer diameter, 4km each length fiber products. Experiments were investigate the characteristics of signal sensitivity according to applied intrusion weight. The relation between the applied weight and the bending loss was almost linear, and broad-area detect intrusion effects are the 2m resolution and $1.3m^2$ per optical fiber length respectively. The light loss by the applied weight on fiber was 0.17 dB/kg. that the sensitivity of the optical fiber sensor was sufficient to detect intruders passing over the buried optical fiber.

• Journal of The Korean Society of Grassland and Forage Science
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• v.39 no.4
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• pp.292-297
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• 2019

In this study, whole crop rice samples were used to develop near-infrared reflectance (NIR) equations to estimate six forage quality parameters: Moisture, crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), Ash and relative feed value (RFV). A population of 564 whole crop rice representing a wide range in chemical parameters was used in this study. Undried finely chopped whole crop rice samples were scanned at 1 nm intervals over the wavelength range 680-2500 nm and the optical data recorded as log 1/Reflectance (log 1/R). NIRS calibrations were developed by means of partial least-squares (PLS) regression. The correlation coefficients of cross-validation (R²_cv) and standard error of cross-validation (SECV) for whole crop rice calibration were 0.98 (SECV 1.81%) for moisture, 0.89 (SECV 0.50%) for CP, 0.86 (SECV 1.79%) for NDF, 0.89 (SECV 0.86%) for ash, and 0.84 (SECV 5.21%) for RFV on a dry matter (%), respectively. The NIRS calibration equations developed in this study will be useful in predicting whole crop rice quality for these six quality parameters.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.28 no.2
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• pp.217-222
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• 2010

The geoid is the level surface that closely approximates mean sea level and usually used for the origin of vertical datum. For the computation of geoid, various sources of gravity measurements are used in South Korea and, as a consequence, the geoid models may show different results. however, a limited analysis has been performed due to a lack of controlled data, namely the GPS/Leveling data. Therefore, in this study, the gravimetric geoids are compared with the geodetic geoid which is obtained through the GPS/Leveling procedures. The gravimetric geoids are categorized into geoid from airborne gravimetry, geoid from the terrestrial gravimetry, NGII geoid(geoids published by National Geographic Information Institute) and NORI geoid(geoi published by National Oceanographic Research Institute), respectively. For the analysis, the geometric geoid is obtained at each unified national control point and the difference between geodetic and gravimetric geoid is computed. Also, the geoid height data is gridded on a regular $10{\times}10-km$ grid so that the FFT method can be applied to analyze the geoid height differences in frequency domain. The results show that no significant differences in standard deviation are observed when the geoids from the airborne and terrestrial gravimetry are compared with the geomertric geoid while relatively large difference are shown when NGII geoid and NORI geoid are compared with geometric geoid. Also, NGII geoid and NORI geoid are analyzed in frequency domain and the deviations occurs in long-wavelength domain.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.2
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• pp.224-232
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• 2008

The health benefits associated with tea consumption have resulted in the wide inclusion of green tea extracts in botanical dietary supplements, which are widely consumed as adjuvants for complementary and alternative medicines. Tea contains polyphenols such as catechins or flavan-3-ols including (-)-epicatechin (EC), (-)-epigallocatechin (EGC), (-)-epicatechin gallate (ECG), and (-)-epigallocatechin gallate (EGCG), as well as the alkaloid, caffeine. The contents of catechins and caffeine in green tea are considered as a standard of quality evaluation of green tea. Therefor, the purpose of this study was to investigate the most suitable HPLC condition for simultaneous determination of catechins and caffeine in green tea extracts. The efficient HPLC analytical condition of catechins and caffeine contained green tea extracts was developed. The gradient elution employed a $250\;mm\;{\times}\;4.6\;mm$ i.d. YMC-pak ODS-AM 303 column. The gradient system was used two mobile phases. A gradient elution was performed with mobile phase A, consisting of 0.1% aqueous phosphoric acid, and mobile phase B, comprising 100% MeOH, and delivered at a flow rate of 1 mL/min as follows: $0{\sim}25\;min$, 80% A; $26{\sim}50\;min$, $80{\sim}70%$ A; 51 min, 80% A. $51{\sim}55\;min$, 80% A. The UV detection wavelength was set at 280 nm. The limit of detection (LOD) for catechins and caffeine standards were under 50 ng/mL.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.11
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• pp.1682-1686
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• 2015

This study was conducted to establish an HPLC analysis method for determination of marker compounds as part of materials standardization for development of health functional food materials from pear pomace. The quantitative determination method of caffeic acid and chlorogenic acid as marker compounds of pear pomace extract (PPE) was optimized by HPLC analysis using a C18 column ($5{\times}250mm$, $5{\mu}m$) with a 0.2% elution gradient of acetic acid and methanol as the mobile phase at a flow rate of 0.8 mL/min and detection wavelength of 330 nm. The HPLC/UV method was applied successfully to the quantification of marker compounds in PPE after validation of the method with linearity, accuracy, and precision. The method showed high linearity of the calibration curve with a coefficient of correlation ($R^2$) of 0.9999, and limit of detection and limit of quantification were $1.14{\mu}g/mL$ (caffeic acid) and $1.61{\mu}g/mL$ (chlorogenic acid) as well as $4.9{\mu}g/mL$ (caffeic acid) and $4.9{\mu}g/mL$ (chlorogenic acid), respectively. Relative standard deviation values from intra- and inter-day precision were less than 3.1% (caffeic acid) and 4.0% (chlorogenic acid), respectively. Recovery rates of caffeic acid and chlorogenic acid at 12.5, 25, and $50{\mu}g/mL$ were 93.66~106.32% and 97.33~105.68%, respectively. An optimized method for extraction of caffeic acid and chlorogenic acid in PPE was established through diverse extraction conditions, and the validation indicated that the method is very useful for evaluation of marker compounds in PPE to develop a health functional food material.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.21 no.12
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• pp.1343-1351
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• 2010

In this paper, a novel patch antenna based on the metamaterial CRLH(Composite Right- and Left-Handed) structure is designed, implemented, and measured. Contrary to the standard microstrip patch's fundamental resonance mode of half-wavelength or its positive multiple, the proposed antenna shows the in-phase electric field over the entire antenna. The proposed antenna has a desired omni-directional field pattern which is typical characteristic of $\lambda/4$ monopole antenna, and also shows the merit of low profile. HFSS(High Frequency Structure Simulator) of Ansoft which is based on the FEM(Finite Element Method) is used to simulate the proposed antenna. FR-4 substrate of thickness 1.6 mm and relative permitivity 4.4 is used for the proposed antenna implementation. The implemented antenna showed VSWR (Voltage Standarding Wave Ratio)$\leq$2 for the frequency band from 2.63 GHz to 2.655 GHz which is used for S-DMB (Satellite-Digital Multimedia Broadcasting) service. And measured peak gain and efficiency are 2.65 dBi and 81.14 %, respectively.

• The Bulletin of The Korean Astronomical Society
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• v.41 no.1
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• pp.54.1-54.1
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• 2016

Multi-wavelength variability is a staple of active galactic nuclei (AGN). Optical variability probes the nature of the central engine of AGN at smaller linear scales than conventional imaging and spectroscopic techniques. Previous studies have shown that optical variability is more prevalent at longer timescales and at shorter wavelengths. Intra-night variability can be explained through the damped random walk model but small samples and inhomogeneous data have made constraining this model hard. To understand the properties and physical mechanism of intra-night optical variability, we are performing the KMTNet Active Nuclei Variability Survey (KANVaS). Using KMTNet, we aim to study the intra-night variability of ~1000 AGN at a magnitude depth of ~19mag in R band over a total area of ${\sim}24deg^2$ on the sky. Test data in the COSMOS, XMM-LSS, and S82-2 fields was obtained over 4, 6, and 8 nights respectively during 2015, in B, V, R, and I bands. Each night was composed of 5-13 epoch with ~30 min cadence and 80-120 sec exposure times. As a pilot study, we analyzed data in the COSMOS field where we reach a magnitude depth of ~19.5 in R band (at S/N~100) with seeing varying between 1.5-2.0 arcsec. We used the Chandra-COSMOS catalog to identify 166 AGNs among 549 AGNs at B<23. We performed differential photometry between the selected AGN and nearby stars, achieving photometric uncertainty ~0.01mag. We employ various standard time-series analysis tools to identify variable AGN, including the chi-square test. Preliminarily results indicate that intra-night variability is found for ~17%, 17%, 8% and 7% of all X-ray selected AGN in the B, V, R, and I band, respectively. The majority of the identified variable AGN are classified as Type 1 AGN, with only a handful of Type 2 AGN showing evidence for variability. The work done so far confirms there are more variable AGN at shorter wavelengths and that intra-night variability most likely originates in the accretion disk of these objects. We will briefly discuss the quality of the data, challenges we encountered, solutions we employed for this work, and our updated future plans.