• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.289-289
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• 2022

Waterlogging tolerance of corn is one of the important factor for cultivate in paddy soil condition to increase cultivation area and self-sufficiency of corn in Korea. In order to develop elite waterlogging tolerance corn, the new corn lines bred by crossing wild corn, Teosinte, and cultivated corn inbred lines. Five accessions among the 2 species, Zea mays sub spp. mexicana and Zea mays spp. parviglumis, of 81 Teosinte were selected through the waterlogging treatment. The waterlogging treatments were implemented for 7 days at the seedling(V3) stage. The inbred lines were developed by crossing 5 teosinte accessions and cultivated corn lines and they were estimated waterlogging tolerance. It was screened and analyzed the metabolites extracted from roots of 19KT-32(KS141 × teosinte) that was treated waterlogging. We selected 8 of 180 metabolites like as γ-aminobutyric acid(GABA), putrescine, citrulline, Gly, and Ala that expression was remarkably changed over 2.5-times, 7 metabolites increased and 1 metabolite decreased in waterlogging, respectively. Glutamate decarboxylase(GAD) catalyzing GABA accumulation gene have 10 haplotypes, and exon1 was highly conserved, but identified to 135 SNPs after the first intron. Among the 135 SNPs, the number of transversion mutations (52) surpassed the number of transition mutations (38). Most of metabolites were related to abiotic stress in plant that it regulated to pH, osmotic pressure K⁺/Ca⁺⁺ and ATPase activity. We are analyzing the association using these results for increase breeding efficiency.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.229-229
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• 2022

Rapeseed is a crop that is waterlogging sensitive, and it is necessary to breed waterlogging tolerance varieties. Our study presents the comparative transcriptome changes in two rapeseed lines, i.e., waterlogging-tolerant (tJ8634-B-30,) and - sensitive ('EMS26') lines under control and waterlogging stress treatments at the flowering stage. RNA-sequencing analysis revealed 13,279 differentially expressed genes (DEGs) for 'J8634-B-30' and 8,682 DEGs for 'EMS26' under waterlogging stress condition compared to control. Among DEGs of 'J8634-B-30', 6,818 were up-regulated and 6,461 were down-regulated. On the other hand, among the DEGs of 'EMS26', the number of down-regulated genes (5,240) were higher than that of up-regulated genes (3,442). Gene ontology enrichment analysis showed that DEGs related to glucan metabolic, cell wall, and oxidoreductase activity were significantly changed in 'J8634-B-30'. Kyoto Encyclopedia of Genes and Genomes (KEGG)-based analysis in 'J8634-B-30' identified up-regulated DEGs being involved in MAPK signaling pathways. In addition, the DEGs belonging to mechanisms responding to waterlogging stress, i.e., plant hormones, carbon metabolism, Reactive oxygen species (ROS), Nitric oxide (NO) etc. were compared in rapeseed lines. Several DEGs including ethylene-responsive transcription factor (ERF), constitutive triple response (CTR) (in ethylene signaling pathway), monodehydroascorbate Reductase (MDAR), NADPH oxidase (in ROS pathway), cytochrome c oxidase assembly protein (COX) (in NO pathway) up-regulated in 'J8634-B-30'. These outcomes provided the valuable information for further exploring the genetic mechanism of waterlogging tolerance in rapeseed.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.281-281
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• 2017

Recently, global warming by greenhouse gas effect is getting danger and danger for human life and agriculture at present. In Indonesia, according to heavy rain in the agriculture land is often covered by excess water in result crop growth would be affected negative. This water stress triggers roots failure in anaerobic condition for upland crop because of limiting roots respiration. Chili pepper grows in upland sometimes in touch with waterlogging due to rainfall and /or over flow water from river in Indonesia. In this case, roots growing is inhibited by effect of shortage of oxygen at root cap. Therefore, the objective of this study is to observe the plant behavior in waterlogging using mahor local genotypes (Ferosa, Laris, Romario) in Sumatra. The experiment was kept by at 1cm depth water above the soil surface as a waterlogged treatment for ---days. As a result, waterlogging affected plant growth of chili negatively, especially for roots growth. Almost roots were getting bad and changed color for brown during waterlogging. A significant negative effect for nutrient absorption by roots was found in dry weight of all varieties during waterlogging. Dry weight of roots was decreased by 81.4% and 67.6%, and those of aerial part decreased by 74% and 67.2% compared with control in Ferosa and Romario at 1week after treatment. On the other hand, dry weight of roots was decreased only 35% in Laris. Therefore, Laris has a tolerance for waterlogging compared to with other varieties. Also, Laris in SPAD value was kept initial level during waterlogging however those of Ferosa and Romario decreased. Finally, due to impact of waterlogging, it may be the roots become failure because of less aerenchyma formation under anaerobic condition. We need confirm aerenchyma formation morphologically in the future.

• Journal of Crop Science and Biotechnology
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• v.11 no.2
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• pp.111-118
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• 2008

Soybean is sensitive to waterlogging stress, leading to reduce their growth and yield significantly. The objective of this study was to characterize the relative sensitivities of biomass accumulation and specific nodule activity under waterlogging stress between supernoduating mutants, 'SS2-2' and 'Sakukei 4' and their wild-type soybeans, 'Sinpaldalkong 2' and 'Enrei', respectively. Flooding treatment was performed to soybean plants grown in a pot by waterlogging for 15 days from the beginning bloom(R1) stage under natural light. The nodule number and weight were considerably decreased by waterlogging stress. The bleeding sap rate of waterlogging soybean plants was decreased by 78-80% in supernodulating mutants and 65-74% in their wild types compared to control plants. The relative ureide-N content was also decreased by waterlogging and the reduction was high in supernodulating mutants. This may cause the massive reduction of shoot and root dry weight and leaf area in waterlogged soybean plants. There was a varietal difference in response to the waterlogging stress. During the waterlogging, supernodulating mutants maintained higher spad value than their wild types. Particularly, the difference between soybean varieties was clear in low rank leaves from the top. Also, supernodulating mutants showed a weak waterlogging tolerance than their wild types. Under waterlogging conditions, massive nodules were considerably destroyed and specific nodule activity after waterlogging may not be recovered when compared to their wild-type soybeans. Supernodulating mutants showed lower seed yield than their wild types in waterlogging conditions.

• Journal of Plant Biotechnology
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• v.49 no.3
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• pp.240-249
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• 2022

Measuring chlorophyll fluorescence (CF) is a useful tool for assessing a plant's ability to tolerate abiotic stresses such as drought, waterlogging and high temperature. Korean ginseng is highly sensitive to water stress in paddy fields. To evaluate the possibility of non-destructively diagnosing waterlogging stress using chlorophyll fluorescence (CF) imaging techniques, we screened 57 ginseng accessions for waterlogging tolerance. To evaluate waterlogging tolerance among the 2-year-old Korean ginseng accessions, we treated ginseng plants with water stress for 25 days. The physiological disorder rate was characterized through visual assessment (an assigned score of 0-5). The physiological disorder rates of Geumjin, Geumsun and GS00-58 were lower than that of other accessions. In contrast, lines GS97-62, GS97-69 and GS98-1-5 were deemed susceptible. Root traits, chlorophyll content and the reduction rates decreased in most ginseng accessions. Further, these metrics were significantly lower in susceptible genotypes compared to resistant ones. All CF parameters showed a positive or negative response to waterlogging stress, and this response continuously increased over the treatment time among the genotypes. The CF parameter Fv/Fm was used to screen the 57 accessions, and the results showed clear differences in Fv/Fm between the susceptible and resistant genotypes. Susceptible genotypes had an especially low Fv/Fm value of less than 0.8, reflecting damage to the reaction center of photosystem II. It is concluded that Fv/Fm can be used as a CF parameter index for screening waterlogging stress tolerance in ginseng genotypes.

• Journal of The Korean Society of Grassland and Forage Science
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• v.37 no.4
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• pp.271-276
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• 2017

Waterlogging strongly affects maize (Zea mays L.) growth. It is necessary to find the screening method of waterlogging tolerant maize lines. This study was to investigate the growth characters at V3 stage of maize, when is very sensitive to waterlogging. Six Korean maize inbred lines were subjected to waterlogging at V3 stage for 30 days. The 30 days waterlogging treatment significantly reduced plant height, number of expanded leaves, and SPAD value, compared with the control plants. SPAD values were significantly different among the six inbred lines, KS140 was the highest. The dry matter accumulation of aerial and root part were significantly decreased by 30 days waterlogging. KS140 was the weightiest among inbred lines. The dry matter of adventitious root showed same trend. Waterlogging treatment significantly reduced to ear length and thickness, grains filling length, grain number per ear, and maize grain. Plant height, SPAD value, and number of fully-expanded leave showed high correlation with maize grain yield, but number of senescent leaves, dry matter of adventitious root and TR ratio did not, suggesting that the former three traits may be good indicator for evaluating 30-day waterlogging tolerance of maize inbred lines. KS164 was the highest yield by increasing of grains filling length and grain number per ear of among waterlogging inbred lines. According to the results, evaluation of maize waterlogging should be consider both early growth characteristics and resilience in the later growth stages.

• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.1
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• pp.13-18
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• 2009

This experiment was carried out to know adaptability and forage production and quality of corn hybrid for silage at paddy field from 2007 to 2008 at JullaNamdo Agricultural Research and Extension Services (ARES). Among agronomic characteristics, 'Kwangpyongok' hybrid was somewhat strong for waterlogging and good stay green, lodging, disease and insect resistance. The fresh yield and total digestible nutrients (TDN) of 'Kwangpyongok' hybrid were the highest among corn hybrids. Dry yield of 'Kwangpyongok' hybrid was increased to 50.5%, 13.6% due to waterlogging tolerance than those 'P3156' and 'P32P75' hybrids, respectively. The result of this study indicated that 'Kwangpyongok' hybrid could be recommended as having good characters according to production, waterlogging tolerance, stay green at paddy field in southern region of Korea.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.65 no.2
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• pp.104-112
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• 2020

Maize is thought to be an alternative crop to rice in paddy fields for efficient field management and maintenance of rice production at appropriate levels in Korea. Thus efforts to breed waterlogging-tolerant maize cultivars have been ongoing. However, molecular studies related to waterlogging tolerance are limited for developing molecular markers to select waterlogging tolerant maize varieties. In this study, we examined molecular biological changes of B73 in the V3 stage after immersion treatment for 7 days. Overall growth of maize was lower in treated samples compared to non-immersed control samples. The length of leaf and root decreased by 21.3% and 50.6%, respectively and the weight of root reduced by 21.6%. Soil plant analysis development (SPAD) value and chlorophyll content of leaf also decreased by 55.7% and 35.3%, respectively. Reactive oxygen species (ROS) content of root increased by 46.5% at 2 hours in immersion treatment. In addition, immersed roots were 2.5-fold thickened with additional aerenchyma formation in the cortex. In order to identify the causes of these changes, we performed a microarray and found increased expression of genes, such as WIP1, PMIP2, EXPA1, TPS1, and MAS1, in immersed samples. These differentially expressed genes and expression of previously reported genes, including ALDH2, Wusl1032, UP-1, UP-2, and CAT2 were further confirmed with qRT-PCR. Here, we report 7 differentially expressed genes after immersing treatment, which may be utilized as useful resources for breeding waterlogging- tolerant maize.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.56 no.4
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• pp.385-393
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• 2011

Soybean is the most promising crop for substituting rice on the paddy field. Excessive water stress is a common limiting factor in soybean yield under paddy soil condition. This study was carried out to identify changes in leaf total nitrogen and petiole ureide content under excess water conditions for establishing a screening system related to waterlogging tolerance. Waterlogging treatment was conducted by maintaining the water level on the soil surface for 10 days at the early vegetative growth stage ($V_5$) and the flowering stage ($R_2$). Leaf total nitrogen content, SPAD value and ureide content in petiole decreased in all soybean varieties in response to waterlogging, but the degree of decrease was much lesser in Pungsannamulkong and Muhankong than in Jangyeobkong and Myungjunamulkong, at 21 days after waterlogging treatment. This result means that root and nodule recovery rates were much higher in Pungsannamulkong and Muhankong than in Jangyeobkong and Myungjunamulkong after waterlogging treatment. The ureide and leaf nitrogen content showed high positive correlation with SPAD value, regardless of waterlogged stages. In conclusion, leaf nitrogen content, ureide content in petiole and leaf greenness were identified as promising indicator for screening soybeans which are tolerant of excess water.

• Proceedings of the Korean Society of Crop Science Conference
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• 2018.04a
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• pp.165-165
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• 2018