• 한국약용작물학회지
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• 제23권4호
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• pp.292-297
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• 2015

This study was carried out to have the basic and applied informations relating to develop the cultivation methods and to increase the productivity and quality of ginseng. 1 to 6 year old ginsengs of Jakyung cultivar were cultivated and the content and synthetic amount of carbohydrates were investigated with different plant tissues, growth stages, and years old. The concentration of total carbohydrates at six year old ginseng including water soluble and water insoluble carbohydrates was about 18.9%, 42.9%, and 43,6% in leaves, tap roots, and lateral roots, respectively. Water soluble carbohydrate of tap and lateral roots was slightly decreased from August until September, and then increased on November, whereas its water insoluble carbohydrate was increased from August to September and then decreased on November. Comparing with the content of carbohydrates of 1 to 6 year old ginsengs, it was continuously increased from one year old ginseng until five year old ginseng, however it was not increased much in six year old ginseng. The highest content of carbohydrates was at five year-old in all tissues of ginseng. Water soluble and water insoluble carbohydrates were significantly shown different in leaves, stems, tap roots, and lateral root at different growth stages and with different years old. The content of water soluble carbohydrate in the leaves was remarkedly higher compared to that of water insoluble carbohydrate, while in the root the content of water insoluble carbohydrate was clearly higher compared to the water soluble carbohydrate. Comparing with the synthetic amount of carbohydrates, water soluble carbohydrates was higher in the shoot than that in the root, whereas water-insoluble carbohydrates higher in the root than that in the shoot. Carbohydrates which would be utilized in ginseng tissues for short and long-term periods as major energy were appeared differently in between shoot and root, with different growth stages, and years old.

• 한국토양비료학회지
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• 제47권6호
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• pp.563-570
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• 2014

The shortage or surplus of minerals directly affects overall physiological metabolism of plants; especially, it strongly influences carbohydrate metabolism as a primary response. We have studied mineral uptake, synthesis and partitioning of soluble carbohydrates, and the relationship between them in N, P or K-excessive tomato plants, and examined the interaction between soluble carbohydrates and mineral elements. Four-weeks-old tomato plants were grown in a hydroponic growth container adjusted with excessive N ($20.0mmol\;L^{-1}$ $Ca(NO_3)2{\cdot}4H_2O$ and $20.0mmol\;L^{-1}$ $KNO_3$), P ($2.0mmol\;L^{-1}$ $KH_2PO_4$), and K ($20.0mmol\;L^{-1}$ $KNO_3$), respectively, for 30 days. Shoot growth rates were significantly influenced by excessive N or K, but not by excessive P. The concentrations of water soluble N (nitrate and ammonium), P and K were clearly different with each tissue of tomato plants as well as the mineral conditions. The NPK accumulation in all treatments was as follows; fully expanded leaves (48%) > stem (19%) = roots (16%) = petioles (15%) > emerging leaves (1). K-excessive condition extremely contributed to a remarkable increase in the ratio, which ranged from 2.79 to 10.34, and particularly potassium was dominantly accumulated in petioles, stem and roots. Fresh weight-based soluble sugar concentration was the greatest in NPK-sufficient condition ($154.8mg\;g^{-1}$) and followed by K-excessive (141.6), N-excessive (129.2) and P-excessive (127.7); whereas starch was the highest in K-excessive ($167.0mg\;g^{-1}$) and followed by P-excessive (146.1), NPK-sufficient (138.2) and N-excessive (109.7). Soluble sugar showed positive correlation with dry weight-based total N content (p<0.01) whereas was negatively correlated with soluble P (p<0.01) and dry weight-based total P (p<0.01). On the other hand, starch production was negatively influenced by total N (p<0.001), but, it showed positive relation with total K concentration (p<0.05). This study shows that uptake pattern of NPK and production and partitioning of soluble carbohydrate were substantially different from each mineral, and the relationship between water soluble- and dry weight-based-mineral was positive.

• 한국작물학회지
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• 제45권5호
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• pp.288-293
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• 2000

Two rice cultivars (Oryza sativa L.), Hwa-seongbyeo of Japonica type and Taebackbyeo of Indica/Japonica type, were cultivated with hydroponic culture to examine nitrogen effects on the growth responses, contents and utilizations of carbohydrates, and the ripening velocity of grains with three different N levels. Plant height and tiller number were clearly increased to 80 ppm N level compared to 40 ppm N level and then they were slightly decreased in N level of 120 ppm. Higher dry weights were appeared with 80 ppm N level than did with other N levels, showing statistically differences in both cultivars and N levels, while dry weight of roots was heavier with decreasing the N levels. Therefore, T/R ratios were not significantly different among N levels, although there was statistically differences between rice cultivars. After the flowering stage, higher water-soluble carbohydrate (WSC) and water-insoluble carbohydrate (WISC) were contained in stem compared with other parts, showing that WISC of sheath and stem, unlike WSC, was significantly different among N levels. Starch of grain, WISC, was remarkedly increased from 3.0% at just after the flowering to 52.0% and 75.0% at 15 and 30 day after the flowering, respectively, showing that lower N application had faster accumulation of starch in rice grains. N would affect the contents of carbohydrates of each tissue, and starch accumulation in rice grains.

• 미생물학회지
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• 제6권2호
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• pp.41-53
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• 1968

Studies on the carbohydrate metabolism of yeast as influenced by gamma-irradiation from cobalt-60 have been carried, then the mechanisms of radiation effect on respiration and fermentation were discussed under considerations of permeable changes of irradiated cell membrane. The cells of baker's yeast (Saccharomyces cerevisiae) which had been gamma-irradiated of 240 k.r. doses for an hour, then were put into aerobic oxidation and anaerobic fermentation without substrate. Total and fractionated carbohydrates of irradiated yeast cells were determined by calorimetric method with anthrone and orcinol reagents, the amounts of total carbohydrate, trehalose, RNA-ribose, PCA-soluble glycogen, alkali-soluble glycogen, acetic acid-soluble glycogen, mannan and glucan were determined according to the course of aerobic oxidation and anaerobic fermentation. It is found that the carbohydrates of irradiated cells leak out and amount of the losses teaches eleven times more than that of control, the volume of losses are seems to be replaced by water, it can be suggested the damage of gamma-irradiation occurs in the site of passive transport of cell membrane. The endogeneous aerobic respiration of irradiated cells are increased much more than control, the synthesis of reserve glycogen, glucan and RNA-ribose promoted much more than control. The anaerobic fermentation of irradiated cells are also increased than that of control, but the breakdown of carbohydrate is less than endogeneous respiration of irradiated cells. The synthetic rate is also less than that of aerobic oxidation. In irradiated yeast cells, trehalose is revealed to be primary substrate for endogeneous carbohydrate metabolism, so it is proved that the enzymic patterns are not changed but the activities of enzymes relating endogeneous respiration and autofermentation is activated. It is to be considerable to distiguish endogeneous respiration and autofermentation from exogeneous respiration and fermentation on irradiation, for membrane permeability changes and loses out carbohydrate by ionizing radiation.

• 한국임산에너지학회:학술대회논문집
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• 한국산림바이오에너지학회 1999년도 정기총회 및 학술연구발표회
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• pp.41-42
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• 1999

• Asian-Australasian Journal of Animal Sciences
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• 제24권5호
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• pp.636-642
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• 2011

The effect of different rates of ethanol additive on fermentation quality of napiergrass (Pennisetum purpureum) and residual water soluble carbohydrate were studied in the experiment. The addition rate of ethanol was 0%, 1.5%, 2.5%, 3.5%, 4.5% on fresh weight of napiergrass. The laboratory silos were kept in the room, then were opened on 1, 3, 5, 7, 14, 30 days after ensiling and the changes of silage quality were analyzed, respectively. There was a fast and large reduction in pH from the 5th day of ensiling to below 4.2 except for the 4.5% treatment. After five days the pH of silage decreased slowly and the pH of the ethanol additions was lower than the control. Lactic acid content of ethanol treatments increased significantly (p<0.05) from the 5th day of ensiling, reaching the highest value on either the 7th day or 14th day. The ethanol additive inhibited the break down of silage protein and the ammonia nitrogen content of ethanol addition silage was significantly (p<0.05) lower than the control after 30 days of ensiling. Within the initial first day of ensiling the water soluble carbohydrate content declined quickly. The efficiency of water soluble carbohydrate usage was higher in silage with ethanol than in the control. The acetic acid of ethanol treatment was significantly (p<0.05) lower than control on first and 14th day, but there was no significant (p>0.05) difference among the ethanol addition silages. The volatile fatty acids content of silage increased gradually from the first day of ensiling and reached the peak on 14th day or 30th day and the content of ethanol addition treatment was significantly (p<0.05) lower than the control. The experimental results indicated that adding ethanol inhibited the use of protein and water soluble carbohydrate of aerobic bacteria and reduced the silage losses during the early stage of ensiling and thus supplied more fermentation substrate for lactic acid bacteria and improved the fermentation quality of napiergrass.

• 한국환경과학회지
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• 제20권10호
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• pp.1265-1272
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• 2011

In this study, the extraction characteristics of soluble solid from Rumex crispus(Curled dock) was studied from the investigation of the effects of experimental conditions on extraction rate; extraction ratio, composition of extractants, extraction time and pH of extractant, etc. The proximate composition of Rumex crispus was 2.58% crude lipid, 5.59% crude protein, 7.39% crude ash, 6.13% moisture and 78.31% carbohydrate, respectively. Turbidity of extract by distilled water was higher and increased with extraction time and extraction temperature, where as the turbidity didn't increase by ethanol and methanol in 20 folds of extraction ratio. Turbidity was inversely proportional to the extraction ratio for the three extractants at 25$^{\circ}C$ and 1 hour extraction. But turbidity of extract was highest by composition of 50% methanol-water extractant than any other compositions of extractants. Eighteen and fifteen free aminoacids were detected in extracts with distilled water, methanol and ethanol extractant, respectively, and it's contents were order of glutamic acid>proline>aminobutyric acid>alanine. The extraction rate of soluble solid from Rumex crispus was order of distilled water>methanol>ethanol within experimental extraction ratio. In extraction with distilled water, the contents of soluble solid was inversely proportional to the pH of extractant.

• The Korean Journal of Ecology
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• 제13권1호
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• pp.59-66
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• 1990

Changes in water potential, lethal temperature and carbohydrate content in the leaves of wintergreen (Pyrola japonica) during overwintering were investigated. Leaf water potential was kept at -2 bars in the tender stage before October, decreased to -46 bars in the dormancy stage and increased to -2 bars again after dehardening Lethal temperatures of the leaf tissue were $-7^{\circ}C$ in the tender stage and $-7^{\circ}C$ in the dormancy stage, but did not recover up to that of the tender stage during dehardeding. Peak of soluble sugar content coincided with the nadir of the leaf water potential. There were close relationships among daily minimum air-temperature, leaf water potential and lethal temperature in changing patterns during overwintering.

• Toxicological Research
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• 제27권4호
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• pp.261-267
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• 2011

Artemisia iwayomogi, a member of the Compositae, is a perennial herb easily found in Korea and used as a traditional medicine to treat liver disease. AIP1, a water-soluble carbohydrate fraction from Artemisia iwayomogi, showed anti-tumor and immuno-modulating activities in animal studies. A subacute toxicological evaluation of AIP1 was performed for 4 weeks in ICR mice. After administration of AIP1 (0, 20, 100, 500 mg/kg/day), the clinical signs, mortalities, body weight changes, hematology, blood clinical biochemistry, urinalysis, organ histopathology, organ weights and gross finding were examined. The results showed that there were no significant differences in body weight changes, food intakes, water consumptions, or organ weights among different dose groups. Also we observed no death and abnormal clinical signs during the experimental period. Between the groups orally treated with AIP1 and the control group, there was no statistical significance in hematological test or serum biochemical values. Histopathological examination showed no abnormal changes in AIP1 groups. These results suggest that no observed adverse effect level (NOAEL) of the oral administration of AIP1 for 4 weeks was considered to be more than 500 mg/kg/day in mice under the condition investigated in current study.

• 한국작물학회지
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• 제40권5호
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• pp.556-561
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• 1995

등숙기간중 벼의 탄소동화율과 기공전도도 그리고 수용성 탄수화물농도의 내생리듬을 알아보기 위하여 자연상태의 광주기 조건에서 재배된 벼를 일정한 환경조건으로 옮긴 다음 지엽을 대상으로 이들의 반응을 관찰하였다. 자연상태의 광조건에서 성장한 벼의 탄소동화율은 약 24시간을 1주기로 하여 일정한 진폭으로 변화되었으며, 정오에 최고에 달하고 한밤중에 최저에 이르나 72시간이 경화할 때 쯤 변화의 폭은 점차적으로 줄어 들어 120시에는 최고, 최저에 도달하는 시각이 바뀌어 정오에 최저에 달하고 한밤중에 최고에 이르는 현상을 나타내었다. 이와 같은 반응은 기공전도도에서도 찾아볼 수 있어 이들 관계는 매우 밀접함을 보였다. 반면, 개화 후 14일 동안 낮과 밤 모두 일정한 광조건에서 성장한 벼의 탄소동화도율과 기공전도도의 반응은 전자와 같이 주기적인 폭의 변화를 찾아볼 수 없었다. 잎내의 수용성 탄수화물 농도의 변화 또한 탄소동화율과 기공전도도의 변화에서 나타나는 현상과 일치하는 경향을 보였다. 이상에서 탄소동화율과 기공전도도 그리고 수용성 탄수화물의 내생림듬은 작물에 부분적으로 중요한 역할을 하고 있음을 시사하며, 또한 이들은 광주기(photoperiod)에 의존적이기 때문에 광주기가 탄소동화작용 및 수용성 탄수화물 형성에 중요한 조절 요인으로 작용하고 있음을 시사한다.