• Title/Summary/Keyword: Water-saving culture

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Usefulness of microwave to melt rehydrated media and to remove oxygen from anaerobic tube media (Microwave를 이용한 배지 융해와 공기 제거)

  • 정윤섭;이귀녕;이삼열
    • Korean Journal of Microbiology
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    • v.18 no.3
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    • pp.148-152
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    • 1980
  • The microwave of 2450 MHz, generated by a household cooking oven, was evaluated for its applicability to melt various rehydrated media and to remove dissolved oxygen from tubed media for anaerobic culture. The effect on the sterilization of E. coli in selective media was also evaluated. The following results were obtained. 10 The microwave oven was useful in saving time for melting media and in eliminating heat and combustion gas from the laboratory, which were inevitable by-products in the conventional flame method. 2) Dissolved oxygen could be removed without boiling over by exposing the tubes of anaerobic culture medium after putting them in a wire basket in a beaker with water. 30 The count of E. coli during the melting of MacConkey and EMB agar were similar to those treated with open flame. The microwave treatment was not considered a possible mean to replace autoclaving even in these selective media.

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Comparison of Real-Time PCR and Conventional Culture Method for Detection of Cronobacter spp. in Powdered Foods (분말식품에서 Cronobacter spp. 검출을 위한 Real-Time PCR과 배지배양법의 비교검증)

  • Chon, Jung-Whan;Song, Kwang-Young;Kim, Sun-Young;Hyeon, Ji-Yeon;Kim, Yun-Gyeong;Hwang, In-Gyun;Kwak, Hyo-Sun;Seo, Kun-Ho
    • Korean Journal of Microbiology
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    • v.47 no.1
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    • pp.87-91
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    • 2011
  • The aim of this study was to compare the performance of conventional culture and real-time PCR for detection of Cronobacter spp. in powdered foods. Infant formula, baby food and Misugaru inoculated with Cronobacter were enriched in distilled water as first enrichment step, followed by incubating in Enterobacteriaceae enrichment (EE) broth as second enrichment step. A loopful of enriched sample was streaked onto Druggan-Forsythe-Iversen agar, followed by incubating at $37^{\circ}C$ for 24 h. One milliliter of the enriched distilled water and EE broth were used in real-time PCR assay. No statistical differences were observed in the number of positive samples between culture method and real-time PCR (p>0.05) in all types of food samples. The number of positives of real-time PCR was higher in the first enrichment media (distilled water) than the second enrichment media (EE broth), though there was no significant difference (p>0.05). It appears that some components of the second enrichment broth, EE broth, inhibit the reaction of real-time PCR. These results show that real-time PCR using a single enrichment with distilled water could be useful as an effective screening method for detection of Cronobacter while saving much time and labor compared to conventional culture method.

Design for Carbon Neutral Arboretum in Gwangju Metropolitan City (광주광역시 탄소중립 수목원 설계)

  • Kim, Hoon Hee
    • KIEAE Journal
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    • v.9 no.3
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    • pp.61-68
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    • 2009
  • Gwangju Metropolitan Government & Ministry of Environment have signed a model city in response to Climate Change agreement. The agreement calls for Gwangju to cut greenhouse gas emissions 10% below 2005 levels by 2015. Gwangju has seen this agreement as an opportunity to cut pollution and conserve the environment as well as to reinvigorate local economy. According to policy of Gwangju, Gwangju held design competition for Gwangju City Arboretum on march, 2009. The purpose of design competition was to give a wide publicity to Gwangju as Hub City of Asian Culture and construct carbon-neutral arboretum in accordance with the policy of 'Low-Carbon and Green Growth'. First of all, a design concept of arboretum is 'winding, round, overlay 'to reflect the landscape of Nam-do which is surrounded by mountains and river flows through the village. Second, the arboretum has five different places with these themes - Forest of Festivals, Health, Nature, Nostalgia, Education and Future. Each place has a symbolic theme park and different flow planning respectively. Third, the most critical point is that the arboretum is a carbon-neutral park. Gwangju arboretum will soon be developed in metropolitan sanitary landfill and constructed as the O2 arboretum based on low carbon strategy. Fourth, the O2 arboretum suggests specialized issue : 'Energy Saving', 'Recycling System', 'Green Network', 'Water System(rainwater maintenance and wetland development)'. Besides, main buildings(greenhouse, visitor center, Nam-do experience exhibition hall, and forest museum) is designed in consideration of harmony with topography character, surroundings. Also, planting will be a multilayer plant based on native landscape trees in consideration of function and the growth characteristics.

Microbial Differentiation and its Biochemical Bases (미생물의 분화와 그 생화학적 기구)

  • 김종협
    • Korean Journal of Microbiology
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    • v.11 no.2
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    • pp.101-106
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    • 1973
  • The microwave of 2450 MHz, generated by a household cooking oven, was evaluated for its applicability to melt various rehydrated media and to remove dissolved oxygen from tubed media for anaerobic culture. The effect on the sterilization of E. coli in selective media was also evaluated. The following results were obtained. 10 The microwave oven was useful in saving time for melting media and in eliminating heat and combustion gas from the laboratory, which were inevitable by-products in the conventional flame method. 2) Dissolved oxygen could be removed without boiling over by exposing the tubes of anaerobic culture medium after putting them in a wire basket in a beaker with water. 30 The count of E. coli during the melting of MacConkey and EMB agar were similar to those treated with open flame. The microwave treatment was not considered a possible mean to replace autoclaving even in these selective media.

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Mycoherbicidal Potential of Phaeoacremonium italicum, A New Pathogen of Eichhornia crassipes Infesting Harike Wetland, India

  • Singh, Birinderjit;Saxena, Sanjai;Meshram, Vineet;Kumar, Maneek
    • Mycobiology
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    • v.44 no.2
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    • pp.85-92
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    • 2016
  • Mycoherbicides are exclusive biotechnology products which offer a non-chemical solution to control noxious weeds on the land as well as aquatic in systems, viz a viz saving environment from hazardous impact of synthetic chemicals. The present paper highlights the mycobiota associated with Eichhornia crassipes infesting Harike wetland area of Punjab and evaluation of their pathogenic potential for futuristic application as a mycoherbicide. Of the 20 isolates tested by leaf detached assay and whole plant bioassays, only one isolate (#8 BJSSL) caused 100% damage to E. crassipes. Further, the culture filtrate of this isolate also exhibited a similar damage to the leaves in an in vitro detached leaf assay. The potential isolate was identified as Phaeoacremonium italicum using classical and modern molecular methods. This is the first report of P. italicum as a pathogen of E. crassipes and of its potential use as a biological control agent for the management of water hyacinth.

Analysis of Nutrients Balance during Paddy Rice Cultivation (수도재배시 논에서의 영양물질 수지 분석)

  • Hwang, Ha-Sun;Jeon, Ji-Hong;Ham, Jong-Hwa;Yoon, Chun-Gyeong
    • Korean Journal of Ecology and Environment
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    • v.36 no.1 s.102
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    • pp.66-73
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    • 2003
  • Field experimental study was performed to evaluate water and nutrient balances in paddy rice culture. The water balance showed that outflow generally balanced the inflow showing that about half (47${\sim}$54%) of total outflow was lost by surface drainage. No significant effect of fertilization rate was observed from three treatments (150%, 100%, and 70% of standard fertilization) on both rice yield and nutrient outflow from surface drainage. Therefore, reducing nonpoint source nutrient loading by reducing fertilization may not work well in the range of normal paddy rice farming practice, and instead it could be achieved by reducing surface drainage outflow. Water-saving irrigation by reducing pending depth, raising ridge height in rice field, and minimizing forced surface drainage are suggested to reduce surface drainage outflow. These practices can save water and protect water quality, however, deviation from conventional standard practices might affect the rice yield and further investigations are necessary. It was demonstrated that rainfall affects nutrient net outflow and paddy rice culture might be beneficial to wafer quality protection under normal rainfall condition.

Comparison of detergency effectiveness by the type of household washer, detergent and soil - Focused on detergency, rinsing, fabric damage and tanglement - (가정용 세탁기, 세제 및 오염의 종류별 세탁 성능 비교 - 세탁성, 헹굼성, 섬유손상도, 엉킴도를 중심으로 -)

  • Piao, Shujing;Park, Myung-Ja
    • The Research Journal of the Costume Culture
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    • v.21 no.6
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    • pp.950-960
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    • 2013
  • The purpose of this research is to provide accurate information of household washers and detergents for consumers, so that help the producers who make washing machine and detergent to get basic material and also help consumers to choose washing machine. Experiment was proceed with two type of washers to compare energy consumption, washing performance, rinsing effectiveness further, damage caused by entanglement of laundry and fabric was assessed. Detergent P and T were used to compare the performance related to differences of ingredients of detergent. Soiled fabrics of EMPA 108 set were used to evaluate performance of washing by different types of contamination. A summary of experimental results are : First, for the consumption of water, drum-type washer consumed 53% less than pulsator-type washer. On the other hand, the washing time was almost similar for both these machines, but pulsator-type washer showed shorter progress, implying that power saving was more efficient in this case. Second, the drum-type washer showed better performance for contamination with all types of detergent, but the pulsator-type washer showed better rinsing performance. Third, the drum-type washer performed less data of tangle level and fabric damage. Fourth, detergent "P" exhibited better washing performance than did detergent "T", regardless of the type of soil. And with no limit of detergent variety, water-soluble protein soil showed high removal rate, liposoluble soil especially pigment was hardly removed.

A new Type of the Doma (Cutting Board) for Kimchi (김치용 도마의 새로운 형태)

  • Kim, Joong-Man
    • Journal of the Korean Society of Food Culture
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    • v.1 no.3
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    • pp.211-218
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    • 1986
  • The doma (cutting board) is an essential kitchen utensil. The general doma used at present is not sultable for cutting kimchi-like juicy foods, because It is a nat flat structure. Kimchi juice contains vitamins, organle acids, and lactic acid bacteria, and inhibits Oxidation of flavor of kimchi. In addition, if the juice flows down on the doma during cutting the doma work-table and kimchi bottom is contaminated. In this studies a new type of doma for kimchi. was made in order to minimize the Juice Iou during cutting. This new one baa a two-dimensional function of low due to length slope face and slanted gutter of width. No this new type has been developed worldwide, as far as the present writer is aware. The new one has various effectivenesses: minimixing loss of kimchi juice and contamination of doma work-table and kitchen bottom by kimchi juice, saving water and time to water contaminated cutting board, work-table and diahtowel (hangju). preventing round from falling down frorr doma duing cutting, decreasing damage of cutting the user's finger, cutting by the fixed size and shape. Draining and drying is easy and it is convenient to cut all juicy food.

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Productivity and Profitability for Direct Seeding Culture of Rice in Mid and Southern Regions (중부와 남부지역 벼 건답직파재배의 생산성과 수익성)

  • 이호진;김수형;이석순
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.5
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    • pp.512-518
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    • 1994
  • One of most laborious work in rice farming is transplanting of rice seedling which has been required preparation of nursury bed and care of seedling during one month period. In this research, direct seeding in dry paddy(DS) was practiced to compare with traditional tansplanting(TP) in Suwon and Milyang. Growth stages in DS were delayed as its planting time was about 36 days later than TP. Heading stage of DS at Suwon was delayed about 15 days as compared to transplanting culture. Rice yield in DS was decreased 8.2%(Suwon) and 0.11%(Milyang), repectively. Working-hour saving in DS was about 34.0%(Suwon) and 54.0%(Milyang). Production cost of DS was decreased 19%(Suwon) and 29% (Milyang), repectively. Amount of rice production per a unit working-hour in DS could incresed 37%(Suwon) and 113%(Milyang) compared to that of TP, respectively. Therefore, DS was decreased little in rice yield, but it could save working hour and production cost, significantly. Warm region like Milyang had more advantage in DS than Suwon. But, DS needs varietal selection for better emergence in low temperature, and more research in weed control and water management.

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