• Title/Summary/Keyword: Water-in-oil emulsion

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Effects of Succinylation and Partial Proteolysis of Soybean Protein Isolates on Functional Properties and Protein-Protein Interaction (숙시닐화 및 부분가수분해가 대두단백질 분리물의 기능적 특성과 단백질-단백질 상호작용에 미치는 영향)

  • Lee, Jee-Won;Ha, Jung-Uk
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.18 no.4
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    • pp.410-422
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    • 1989
  • Soybean protein isolates were acylated with succinic anhydride and partially hydrolyzed with trypsin. Chemical modification decreased protein contents of samples and, in amino acid composition, tyrosine was increased comparatively. And lysine was increased remarkably by partial proteolysis. Succinylation and trypsin treatment increased the aqueous solubility and shifted the isoelectric potint that showed high pH-dependence of protein solubility. Protein solubility was influenced by salt concentration such as $NaCl,\;CaCl_2,\;NaNO_3$ and $NaH_2PO_4$. Chemical modification increased the absorption of oil and water, emulsification properties and foam capacity, but decreased foam stability, ultraviolet absorbance and bulk density. Protein-protein Interaction between soybean protein isolates and beef protein increased the emulsifying activity, emulsifying activity index and foaming properties, but it didn't have any influence on emulsion stability.

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Characteristics and Application of Defatted Soybean Meal fractions Obtained by Microparticulation/Air-Classification (초미세분쇄/공기분급을 이용한 탈지대두박 분획물의 특성과 응용)

  • Park, Dong-June;Ku, Kyung-Hyung;Kim, Seung-Ho
    • Korean Journal of Food Science and Technology
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    • v.28 no.3
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    • pp.497-505
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    • 1996
  • Defatted soybean meal (DSM) was microparticulated at cut-off whell speed of 9.000 rpm and air-classified into fine and coarse fractions at air classifying wheel speeds (ACWS) of 21,000, 18,000, 15,000 12,000 and 9,000 rpm stepwisely. Protein and ash content increased while lipid, carbohydrates and dietary fiber content decreased with decreasing ACWS. Amino acid composition and amino acid content of defatted soybean meal were similar to those of raw soybeans with aspartic and glutamic acid, the major amino acids. The yield and particle size increased with decreasing ACWS and mean particle size ranged from $4.9{\mu}m$ to $14.2{\mu}m$. The particles were oval-shaped with sharp corners. Water holding capacity, oil holding capacity and emulsion capacity slightly decreased with decreasing ACWS. Soybean curds formed showed different characteristics depending on the coagulant used and on the substitution ratio of full fat soybean flour with microparticulated DSM. Microparticulated DSM could successfully substitute wheat flour up to 10% level without bring no-ticiable beany flavor in the cakes.

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Characteristics and Application of Soybean Hull Fractions Obtained by Microparticulation/Air-Classification (초미세분쇄/공기분급을 이용한 대두피 분획물의 특성과 응용)

  • Ku, Kyung-Hyung;Park, Dong-June;Kim, Seung-Ho
    • Korean Journal of Food Science and Technology
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    • v.28 no.3
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    • pp.506-513
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    • 1996
  • Soybean hull was microparticulated at cut-off wheel speed of 9,000 rpm and air-classified into fine and coarse fraction at air classifying wheel speeds (ACWS) of 10.000, 15,000 and 20,000 rpm. respectively. Proximate analysis of the coarse and fine fractions of the microparticulated/air-classified soybean hull showed that ash, protein and lipid were enriched in the coarse fractions and carbohydrates in the fine fraction. Dietary fiber were enriched in coarse fractions and dietary fiber and dietary fiber content increased with decreasing ACWS. Mean particle size of fine fractions ranged from $3.1{\mu}m\;to\;5.5{\mu}m$ that of coarse fractions ranged from $9.9{\mu}m\;to\;20.0{\mu}m$ and both increased with decreasing ACWS. The particles were mostly oval-shaped regardless of the fractions. Generally the fraction of low ACWS showed higher viscosity. In water holding capacity, oil absorption and emulsion capacity, there was significant difference between coarse and fine fractions while there was no significant difference as a function of different ACWS. Oil absorption of flying batter was decreased significantly in the fraction of ACWS 15,000. when cakes and cooking were prepared partially substituted with soybean hull fraction, there was no significant changes on their physical and sensory properties up to 10% substitution.

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Preparation and Release Behavior of Atorvastatin Calcuim - Encapsulated Polyoxalate Microspheres (아토르바스타틴 칼슘을 함유한 폴리옥살레이트 미립구의 제조 및 방출거동)

  • Lee, Cheon Jung;Kim, Su Young;Lee, Hyun Gu;Yang, Jaewon;Park, Jin Young;Cha, Se Rom;Lim, Dong-Kwon;Lee, Dongwon;Khang, Gilson
    • Polymer(Korea)
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    • v.38 no.5
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    • pp.656-663
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    • 2014
  • Atorvastatin calcium-loaded polyoxalate (POX) microspheres were prepared by an emulsion solvent-evaporation/ extraction method of oil-in-oil-in-water ($O_1/O_2/W$) for sustained release. We investigated the release behavior according to initial drug ratio, molecular weight ($M_w$) and concentration of POX and concentration of emulsifier. The microsphere was characterized on the surface, the cross-section morphology and the behavior of atorvastatin calcium release for 10 days by scanning electron microscopy (SEM) and high performance liquid chromatography (HPLC). The analysis of crystallization was analyzed to use X-ray diffraction (XRD), differential scanning calorimeter (DSC) and Fourier transform infrared (FTIR). These results showed that the release behaviors can be controlled by preparation conditions.

Physicochemical properties of supercritical carbon dioxide defatted mealworm (Tenebrio molitor) powder and protein isolate (초임계이산화탄소 탈지 밀웜(Tenebrio molitor) 분말 및 분리단백의 이화학적 품질 특성)

  • Kim, Yangji;Kim, Seok Joong
    • Korean Journal of Food Science and Technology
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    • v.52 no.5
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    • pp.516-523
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    • 2020
  • Supercritical carbon dioxide (SCO2) extraction was applied for the defatting of mealworm to prepare defatted powder (DP) and protein isolate (PI) and compare the process to press and hexane extraction, with respect to DP and PI physicochemical properties. SCO2 DP was obtained by extracting 34.40% oil at 41.37 MPa, 40℃ for 180 min, and the product contained 71.66% crude protein, which is similar to that of hexane DP and higher than that of press DP. In using alkali protein extraction to prepare PI from DP, SCO2 was as effective as hexane and better than press. SCO2 produced brighter DP and PI than press, but not as much as hexane. Protein solubility was similar in all DP, with minimum values at pH 5. The highest water adsorption capacity was noticeable for SCO2 PI, and SCO2 DP showed an oil adsorption capacity comparable to that of hexane DP. SCO2 DP and PI had better foaming capacity than press DP and PI and showed superior emulsion activity compared to others.

Preparation of Functional Cosmetics Containing $\beta$-carotene Derived from Recombinant Escherichia coli and Evaluation of Anti-wrinkle Efficacy by Clinical Testing (재조합 대장균 유래 베타카로틴이 함유된 기능성 화장품 제조 및 이의 임상실험을 통한 주름개선 효능 평가)

  • Kim, You-Geun;Lee, Young-Hoon;Kang, Moon-Kook;Lee, Byung-Hak;Yun, Jun-Ki;Kim, Sung-Bae;Kim, Chang-Joon
    • Microbiology and Biotechnology Letters
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    • v.37 no.4
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    • pp.399-404
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    • 2009
  • This paper described the formulation of functional cosmetics and evaluation of anti-wrinkle efficacy in clinical test. Cosmetics were formulated with highly purified $\beta$-carotene obtained from the culture broth of recombinant E.coli cells. Edible oil for solubilizing $\beta$-carotene, vitamine E for long-term storage, detergent/stabilizer (2.0%) for the complete formation of oil/water emulsion, dimethicone (0.35%) for good skin care, and sorbitol for skin moisturizer were also added as ingredients. Physical or chemical degradation of formulated products stored at $0^{\circ}C$, $25^{\circ}C$, or $40^{\circ}C$ was not observed for 60-day testing period. In clinical test, 68% of applicants observed wrinkle decrease after 8-week treatment. This result indicates that newly formulated cosmetics have strong potential for improving wrinkle skin care.

A Study of the Transdermal Permeation of Lotion Formulations Containing Angelica gigas Nakai Extracts in Franz Diffusion Cells (Franz diffusion cell을 이용한 참당귀 추출물 함유한 로션제형의 피부 투과 연구)

  • Kim, Kang Min
    • Journal of Life Science
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    • v.31 no.11
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    • pp.1004-1009
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    • 2021
  • Angelica gigas Nakai (AGN) has been used in Korean herbal medicine for various pharmacological activities, such as to create antioxidant and skin whitening effects. Decursin and decursinol angelate of AGN extracts can be used as potential active drugs and cosmetic ingredients. This study investigated the possibility of topical delivery of AGN extracts using a manufactured emulsion system. Lotion was formulated by using Tefose® and paraffin for the oil phase, Kolliphor RH 40 for the surfactant and solubilizing agent-which showed high solubility in water (0.82 mg/ml)-and a water phase with a carbomer. In vitro skin permeation of decursin and decursinol angelate was determined using a Strat-M® membrane in Franz diffusion cells. Lotion samples as the experimental group (248.08±19.72 ug/cm2) significantly increased the permeation of decursin and decursinol angelate for up to 24 hr compared to the control group (119.18±19.23 ug/cm2). The permeability was also characterized by the flux (penetration rates) and Kp (permeability coefficient) values. The experimental group (17.20±1.23 ug/h/cm2 and 5.73±1.39 cm/h*10-3) had higher flux and Kp than the control group (8.22±1.24 ug/h/cm2 and 2.74±0.51 cm/h*10-3). Lotion with decursin and decursinol angelate of AGN extracts could be used for the topical application of drug and cosmetic products.

Field evaluation of conidia of Beauveria bassiana (Balsamo) Vuillemin strain CS-1 against diamondback moth larvae (포장에서 배추좀나방에 대한 백강균 CS-1의 방제효과)

  • Yoon, Cheol-Sik;Yoon, Tae-Joong;Park, Hyun-Soo;Lee, Sang-Guei;Yoo, Jai-Ki;Lee, Jeang-Oon
    • The Korean Journal of Pesticide Science
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    • v.2 no.2
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    • pp.113-118
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    • 1998
  • The efficacy of conidia of Beauveria bassiana (Balsamo) Vuillemin strain CS-1 that showed high mortality in laboratory and net house was examined against diamondback moth larvae in the field. Conidia ($4{\times}10^{7}$ conidia/ml) were applied to larvae on chinese cabbage in a 1.5% emulsifiable oil-emulsion amended with 4% clay at a volume of 0.55 L/3.3 $m^{2}$. There were no significant differences among plants and replicates in the deposition of spray droplets on water-sensitive papers or of conidia on leaves and larvae. Weather conditions were rainy and cool during first few days, and then hot and dry. Persistence of conidia was equally short on both leaves and larvae. Nevertheless, treatment of B. bassiana potentially reduced larval populations. But the field efficacy was lower than the laboratory efficacy. This reduced efficacy was ascribed to unfavorable environmental conditions.

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Effect of Rheological Properties on Mascara by Water-soluble Gelling Agents (점증폴리머의 레올로지 특성이 마스카라 물성에 미치는 영향)

  • Choi, Sun-Kyung;Roh, Young-Hea;Choo, Jeong-Han;Choi, Yeong-Jin;Kang, Hak-Hee;Lee, Ock-Sub
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.33 no.3
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    • pp.159-163
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    • 2007
  • The relationship between texture and rheological properties of mascara(oil-in-water emulsion) was analyzed in this study. The final mascara product and gelling agents(2.0 wt%) used therein, such as hydroxyethylcellulose(HC), carboxymethylcellulose(CMC), hectorite, sodium magnesium silicate (SMS), hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer(HS), and polyacrylate 13/polyisobutene/polysorbate 20 (PPP), were measured for rheological properties. As a result, HS and PPP showed the highest adhesiveness which were related to the volumizing effect of mascara. The viscosities of HC, SMS, and HS were measured at the stress range of $1{\sim}1,000s^{-1}$. SMS, with the lowest storage modulus range of $100{\sim}1,000s^{-1}$, affected the mascara in terms of smooth texture. The results of this study suggest that the rheology of gelling agents used influences the final texture of the mascara.

The Inhibition of UVA-induced Matrix Metalloproteinase-1 in Human Dermal Fibroblasts and the Improvement of Skin Elasticity by Cirsium setidens Extract (고려엉겅퀴 추출물의 사람 섬유아세포에 있어서 자외선으로 유도된 MMP-1발현 저해와 피부 탄력 개선 효과)

  • Sim, Gwan-Sub;Kim, Jin-Hwa;Lee, Dong-Hwan;Lee, Bum-Chun;Lee, Geun-Soo;Pyo, Hyeong-Bae
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.33 no.3
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    • pp.181-187
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    • 2007
  • In this study, we measured the anti-oxidative activity of Cirsium setidens and investigated its effect on UVA-induced MMP-1 expression in human dermal fibroblats. And then we examined possible improvement in skin elasticity by topical treatment with fomular including Cirsium setidens extract. The ethanol extract of C. setidens showed free anion radical scavenging effect(87.47 % at 1 mg/mL) and superoxide anion radical scavenging effect(61.71 % at 1 mg/mL) in the xanthine/xanthine oxidase system, respectively. At the concentration of 100 ${\mu}g/mL$, C. setidens extract showed 95.54% inhibition on lipid peroxidation of linoleic acid. UVA-induced MMP-1 expression in human dermal fibroblasts was reduced to 54.69 % by treatment with 100 ${\mu}g/mL$ of C. setidens extract. A human clinical study, in which oil-in-water emulsion with C. setidens extract was topically applied, showed significant increase in skin elasticity. These results suggest that the C. setidens extract can be effective anti-aging ingredient for cosmetics applications.