• Journal of Wetlands Research
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• v.25 no.2
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• pp.75-82
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• 2023

Data for measuring the flow rate of streams are used as important basic data for the development and maintenance of water resources, and many experts are conducting research to make more accurate measurements. Especially, in Korea, monsoon rains and heavy rains are concentrated in summer due to the nature of the climate, so floods occur frequently. Therefore, it is necessary to measure the flow rate most accurately during a flood to predict and prevent flooding. Thus, the U.S. Geological Survey (USGS) introduces 1, 2, 3 point method using a flow meter as one way to measure the average flow rate. However, it is difficult to calculate the average flow rate with the existing 1, 2, 3 point method alone.This paper proposes a new 1, 2, 3 point method formula, which is more accurate, utilizing one probabilistic entropy concept. This is considered to be a highly empirical study that can supplement the limitations of existing measurement methods. Data and Flume data were used in the number of holesman to demonstrate the utility of the proposed formula. As a result of the analysis, in the case of Flume Data, the existing USGS 1 point method compared to the measured value was 7.6% on average, 8.6% on the 2 point method, and 8.1% on the 3 point method. In the case of Coleman Data, the 1 point method showed an average error rate of 5%, the 2 point method 5.6% and the 3 point method 5.3%. On the other hand, the proposed formula using the concept of entropy reduced the error rate by about 60% compared to the existing method, with the Flume Data averaging 4.7% for the 1 point method, 5.7% for the 2 point method, and 5.2% for the 3 point method. In addition, Coleman Data showed an average error of 2.5% in the 1 point method, 3.1% in the 2 point method, and 2.8% in the 3 point method, reducing the error rate by about 50% compared to the existing method.This study can calculate the average flow rate more accurately than the existing 1, 2, 3 point method, which can be useful in many ways, including future river disaster management, design and administration.

• Ecology and Resilient Infrastructure
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• v.10 no.3
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• pp.85-96
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• 2023

The study involved the categorization of domestic lakes located in South Korea into three groups based on their salinity levels: upstream reservoirs with salinity less than 0.3 psu, estuarine reservoirs with salinity ranging from 0.3 to 2 psu, and brackish lagoons with salinity exceeding 2 psu. Subsequently, the research assessed variations in the concentrations of total nitrogen (T-N) and total phosphorus (T-P) in the sediment of these lakes using statistical analysis, specifically one-way analysis of variance (ANOVA). Additionally, a laboratory core incubation test was conducted to investigate the benthic nutrient fluxes in Songji lagoon (salinity: 11.80 psu), Ganwol reservoir (salinity: 0.73 psu), and Janggun reservoir (salinity: 0.08 psu) under both aerobic and anoxic conditions. The findings revealed statistically significant differences in the concentrations of T-N and T-P among sediments in the lakes with varying salinity levels (p<0.05). Further post-hoc analysis confirmed significant distinctions in T-N between upstream reservoirs and estuarine reservoirs (p<0.001), as well as between upstream reservoirs and brackish lagoons (p<0.01). For T-P, a significant difference was observed between upstream reservoirs and brackish lagoons (p<0.01). Regarding benthic nutrient fluxes, Ganwol Lake exhibited the highest diffusive flux of NH₄⁺-N, primarily due to its physical characteristics and the inhibition of nitrification resulting from its relatively high salinity. The flux of NO₃^--N was lower at higher salinity levels under aerobic conditions but increased under anoxic conditions, attributed to the impact of salinity on nitrification and denitrification. Additionally, the flux of PO₄^3--P was highest in Songji Lake, followed by Ganwol Lake and Janggun Reservoir, indicating that salinity promotes the diffusive flux of phosphate through anion adsorption competition. It's important to consider the influence of salinity on microbial communities, growth rates, oxidation-reduction processes, and nutrient binding forms when studying benthic diffusive nutrient fluxes from lake sediments.

• Journal of the Daesoon Academy of Sciences
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• v.41
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• pp.63-131
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• 2022

In the context of Daesoon Jinrihoe's The Canonical Scripture, 'Saving Lives' consists of one 44 verse-long chapter. The content covers Kang Jeungsan's authority and foreknowledge, and most of the passages are about the treatment of diseases. Other passages are about relief from natural disasters, the resolution of conflicts in personnel affairs, and wisdom as applied to saving lives. The treatment of diseases focuses on solving the ailments faced by lower classes during that time period. Kang Jeungsan relieved the damage suffered by the people from natural phenomena as caused by the three disasters which resulted from water, fire, and wind. He also worked to solve other difficulties experienced in human society. In addition, the definition of 'wisdom' here is one of being high-seeing and far-thinking in Saving Lives. That is the foundation upon which wisdom can save oneself and others. After comparing each chapter of The Canonical Scripture with the parallel verses from the 6th edition of Daesoon Jeongyeong, the results of this study can be summarized as follows: first, saving lives as performed by Kang Jeungsan became an unprecedented and absolute act of relieving the common people during a time when they were suffering from great harms, hardships, and difficulties in their daily lives during the late Joseon Dynasty. Second, the verses were distributed into seven parts to achieve the purpose indicated by the titles of each section which related to specific powers exhibited by Kang Jeungsan in his interactions with Heaven, Earth and Humanity. Third, the second chapter of 'Saving Lives' includes not only treatment of disease, natural disasters, and hardships, but also relief meant to lessen the burdens people experienced in their daily lives while living within the boundaries of society. This is different from the respective section from the 6th edition of Daesoon Jeongyeong which, by way of contrast, only contained verses concerning the treatment of disease. The contents of 'Saving Lives' in The Canonical Scripture deals with the relief in a wide range of applications and is thereby different from the chapter on healing from the Daesoon Jeongyeong, which merely dealt with the healing of diseases. Therefore, this broader range of meanings can be taken to be a unique feature of The Canonical Scripture.

• The Korean Journal of Zoology
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• v.18 no.2
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• pp.51-60
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• 1975

The present study was performed histologically and histochemically to observe the cutaneous mucous glands in the frog, Rana nigromaculata during metamorphosis. The cutaneous thssues including dermal plicae in the dorsal portions of the frog tadpoles at each metamorphosis stage were fixed in 10% buffered formalin at$4^{\circ}C$, embedded in paraffin wax, sectioned 4 $\mu$m thickness and stained with periodic acid-Schiff(PAS) and alcian blue (AB) at both pH 2.5 and pH 1.0. The results of observation were as follows: 1. The developments of cutaneous mucous glands of the frog tadpole were begun with appearance of gland cell nest in the dermis at metamorphosis XV stage and significant numerical increases could be seen at metamorphosis XX, XXIII and XXIV stages. 2. This cutaneous mucous gland of the frog tadpole could be divided into two types; A-type glands showed strong positivities to both PAS and AB at pH 2.5 in the gland body cells and to PAS in the gland neck cells, and B-type glands at AB pH 2.5 in the gland body cells. 3. In the A-type mucous glands, the reactivities of the glandular epithelial cells to both PAS and AB stain could be first seen at the metamorphosis XIX stage of frog tadpole. The reactivities of the glandular epithelial cells to both PAS and AB pH 2.5 were gradually increased according to the process of metamorphosis after XX stage of metamorphosis. 4. The B-type mucous glands were first seen at the XX stage and the reactivity of the glandular epithelial cells to AB at pH 2.5 was gradually increased according to the process of metamorphosis after XX stage. 5. The A-type and the B-type mucous glands were in the ratio of 99 : 1, 7 : 3 and 5.5 : 4.5 for each of metamorphosis XX, XXI-XXII and XXIII-XXV stages. 6. The remarkable development of the cutaneous mucous glands of the frog tadpoles might be needed to maintain water and electrolyte balances according to the change of way from aquatic life to amphibious.

• Journal of the Daesoon Academy of Sciences
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• v.47
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• pp.65-103
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• 2023

In The Canonical Scripture (典經), the core scripture of Daesoon Thought, the Former World and the Later World are divided into the Era of Mutual Contention and the Era of Mutual Beneficence. This concept of the Former World and the Later World appears in diagrams on I-Ching Studies (易學) in the text titled, The Comprehensive Mirror of Taegeukdo (太極道通鑑). In I-Ching Studies, Anterior Heaven (先天) and Posterior Heaven (後天) are the main concepts in Song Dynasty diagram books on I-Ching Studies. Among the diagrams of I-Ching Studies, Fuxi's Diagram of the Sequence of the Eight Trigrams, Fuxi's Diagram of the Positions of the Eight Trigrams, Fuxi's Diagram of the Sequence of the 64 Hexagrams, and Fuxi's Diagram of the Positions of the 64 Hexagrams correspond to the Anterior Heaven, and King Wen's Diagram of the Sequence of the Eight Trigrams and King Wen's Diagram of the Positions of the Eight Trigrams correspond to Posterior Heaven. In The Comprehensive Mirror of Taegeukdo, the diagrams of I-Ching Studies are reinterpreted according to Daesoon Thought. The Diagram of the Eight Trigrams of King Wen's Era corresponds to King Wen's Diagram of the Eight Trigrams in I-Ching Studies. This diagram was drawn according to the text in Chapter Five of the Treatise of Remarks on the Trigrams. This diagram corresponds to "the Era of the Nobility of Earth (地尊時代)" centered on the trigram kun (坤 / ☷ ground). Fuxi's Diagram of the Positions of the Eight Trigrams in I-Ching Studies corresponds to The Diagram of the Positions of the Eight Trigrams of Fuxi's Era in Daesoon Thought. The most significant feature of this diagram is that the trigrams assigned to the directions of north and south match the hexagram indicating the obstruction of Heaven and Earth. This is hexagram 12 (否), meaning "obstruction," and it depicts no exchange or communication between Yin and Yang. Naturally, this symbolizes mutual destruction overtaking Yin and Yang. Daesoon Thought expresses this as "the Era of the Nobility of Heaven (天尊時代)." The most significant feature of The Diagram of the Eight Trigrams of the Corrected Book of Changes in The Comprehensive Mirror of Taegeukdo is that the trigrams assigned to the directions of south and north are indicative of hexagram 11, Peace on Earth and in Heaven (泰). This is a diagram in which mutual destruction is resolved through the Five Phases because the trigrams for water (坎 / ☵) and fire (離 / ☲) are in a corrected orientation. Therefore, this diagram symbolizes a world "free from Mutual Contention" and "the Era of Human Nobility (人尊時代)." According to the contents of The Canonical Scripture, the Supreme God performed the Reordering Works of the Three Realms to correct the Mutual Contention of the Former World, and as a result, the Mutual Contention of the Former World will give way to the implementation of the Dao of Mutual Beneficence. The Supreme God's Reordering Works of the Three Realms have been completed in the realm of divine beings, but in the Later World, they appear as an Earthly Paradise where the Dao of Mutual Beneficence is realized. The diagram depicting the Later World is The Diagram of the Eight Trigrams of the Era of the Corrected Book of Changes in The Comprehensive Mirror of Taegeukdo.

• Journal of Food Hygiene and Safety
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• v.39 no.1
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• pp.35-43
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• 2024

Numerous methods have been applied to assess the antibacterial effectiveness of hand hygiene products. However, the different results obtained through various evaluation methods have complicated our understanding of the real efficacy of the products. Few studies have compared test methods for assessing the efficacy of hand hygiene products. In particular, reports on ex vivo pig skin testing are limited. This study aimed to compare and characterize the methodologies applied for evaluating hand hygiene products, involving in vitro, ex vivo, and in vivo approaches, applicable to both leave-on sanitizers and wash-off products. Our further aim was to enhance the reliability of ex vivo test protocols by identifying influential factors. We performed an in vitro method (EN1276) and an in vivo test (EN1499 and ASTM2755) with at least 20 participants, against Serratia marcescens or Escherichia coli and Staphylococcus aureus. For the ex vivo experiment, we used pig skin squares prepared in the same way as those used in the in vivo test method and determined the optimal treated sample volumes for sanitizers and the amount of water required to wash off the product. The hand sanitizers showed at least a 5-log reduction in bacterial load in the in vitro test, while they showed little antibacterial activity in the in vivo and ex vivo tests, particularly those with a low alcohol content. For the hand wash products, the in vitro test was limited because of bubble formation or the high viscosity of the products and it showed low antibacterial activity of less than a 1-log reduction against E. coli. In contrast, significantly higher log reductions were observed in ex vivo and in vivo tests, consistently demonstrating these results across the two methods. Our findings revealed that the ex vivo and in vivo tests reflect the two different antibacterial mechanisms of leave-on and wash-off products. Our proposed optimized ex vivo test was more rapid and more precise than the in vitro test to evaluate antibacterial results.

• Korean Journal of Heritage: History & Science
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• v.57 no.3
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• pp.90-101
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• 2024

The test answer sheets submitted by examinees in the Joseon Dynasty were called Sigwon (test papers with answers, 試卷), and Siji (blank test papers, 試紙) were generally prepared by the examinees themselves. At that time, paper was not produced as a standard product, so there was no uniformity in size or manufacturing method. Mulberry paper in the Joseon Dynasty was basically transparent, so various paper processing methods were applied for examinees to write answers on both sides. In order for ink lines to be written smoothly, Dochim (hitting paper with a wooden bat on the stone, 搗砧) or surface processing was treated. We found a 19th-century Siji (試紙) that was processed in a unique way, which led to this study. An unusual Sigwon (試卷) is one by Lee Mangi (李晩耆) from 1848 owned by the National Folk Museum of Korea. We found that an opaque white substance was thickly applied between the papers of this Siji (試紙). Through component analysis using infrared spectrophotometry, fluorescence X-ray spectroscopy, optical and polarizing microscopy, and electron microscopy, this white substance was proved to be rice starch. From these analyses, it is presumed that this Siji (試紙) was made by soaking rice flour in water to remove a significant amount of protein, and then applying wet starch containing a small amount of protein between sheets of paper. In addition, with a Siji (試紙) reproduction experiment, we found that the paper reproduced by this processing method was thick and high in whiteness and opacity. This is believed to be a production method designed to produce double-sided paper without using multiple sheets of paper, which was difficult to obtain at that time. In this study, the material processed between the sheets of paper was disclosed only from < Lee Mangi (李晩耆)'s Sigwon (試卷)(Minsok 71745)>, but this appears to be one of several processing methods to treat the paper during the Joseon Dynasty. We hope that more similar Sigwons will be discovered in the future and that extensive research on processing methods will be conducted.

• Journal of Bio-Environment Control
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• v.32 no.4
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• pp.384-395
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• 2023

Since transpiration plays a key role in optimal irrigation management, knowledge of the irrigation demand of crops like tomatoes, which are highly susceptible to water stress, is necessary. One way to determine irrigation demand is to measure transpiration, which is affected by environmental factor or growth stage. This study aimed to estimate the transpiration amount of tomatoes and find a suitable model using mathematical and deep learning models using minute-by-minute data. Pearson correlation revealed that observed environmental variables significantly correlate with crop transpiration. Inside air temperature and outside radiation positively correlated with transpiration, while humidity showed a negative correlation. Multiple Linear Regression (MLR), Polynomial Regression model, Artificial Neural Network (ANN), Long short-term Memory (LSTM), and Gated Recurrent Unit (GRU) models were built and compared their accuracies. All models showed potential in estimating transpiration with R² values ranging from 0.770 to 0.948 and RMSE of 0.495 mm/min to 1.038 mm/min in the test dataset. Deep learning models outperformed the mathematical models; the GRU demonstrated the best performance in the test data with 0.948 R² and 0.495 mm/min RMSE. The LSTM and ANN closely followed with R² values of 0.946 and 0.944, respectively, and RMSE of 0.504 m/min and 0.511, respectively. The GRU model exhibited superior performance in short-term forecasts while LSTM for long-term but requires verification using a large dataset. Compared to the FAO56 Penman-Monteith (PM) equation, PM has a lower RMSE of 0.598 mm/min than MLR and Polynomial models degrees 2 and 3 but performed least among all models in capturing variability in transpiration. Therefore, this study recommended GRU and LSTM models for short-term estimation of tomato transpiration in greenhouses.

• Microbiology and Biotechnology Letters
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• v.52 no.3
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• pp.255-263
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• 2024

In this study, antioxidant and anti-inflammatory activity was studied to confirm the value of kailan (Brassica oleracea L.) as a natural material for cosmetics. For this study measure the antioxidative activity, total polyphenol content was measured, and DPPH and ABTS scavenging activity assays were conducted. As a result of measuring the total polyphenol content of hot water extract of kailan (KRD) and 70% ethanol extract of kailan (KRE), it was found to contain 124.3 mg TAE/100 g and 144.1 mg TAE/100 g, respectively. As a result of DPPH and ABTS radical scavenging ability, it was confirmed that the efficacy was concentration dependent. After treating the cells with LPS, a stimulant, for 2 hours, an experiment was conducted by treating RAW 264.7 cells with KRD and KRE at concentrations of 10, 50, and 100 ㎍/ml. The nitric oxide production inhibitory activity of KRD and KRE showed an inhibitory effect of about 30% at a concentration of 100 ㎍/ml. Cells cultured for 18 hours after stimulant treatment were obtained and used in experiments. The cells obtained in this way were lysed, protein and mRNA were extracted, and the expression of inflammatory mediators' inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) was confirmed. It was confirmed that the protein mRNA expression of iNOS and COX-2, measured through western blot and reverse transcription-PCR, was inhibited in a concentration-dependent manner. Based on this, it is judged that has the potential to be used as a natural material in cosmetics.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.18 no.1
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• pp.40-46
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• 2013

The isotope ratios of $^{13}C/^{12}C$ and $^{18}O/^{16}O$ for a sample in a mass spectrometer are measured relative to those of a pure $CO_2$ reference gas (i.e., laboratory working standard). Thus, the calibration of a laboratory working standard gas to the international isotope scales (Pee Dee Belemnite (PDB) for ${\delta}^{13}C$ and Vienna Standard Mean Ocean Water (V-SMOW) for ${\delta}^{18}O$) is essential for comparisons between data sets obtained by other groups on other mass spectrometers. However, one often finds difficulties in getting well-calibrated standard gases, because of their production time and high price. Additional difficulty is that fractionation processes can occur inside the gas cylinder most likely due to pressure drop in long-term use. Therefore, studies on laboratory production of pure $CO_2$ isotope standard gas from stable solid calcium carbonate standard materials, have been performed. For this study, we propose a method to extract pure $CO_2$ gas without isotope fractionation from a solid calcium carbonate material. The method is similar to that suggested by Coplen et al., (1983), but is better optimized particularly to make a large amount of pure $CO_2$ gas from calcium carbonate material. The $CaCO_3$ releases $CO_2$ in reaction with 100% pure phosphoric acid at $25^{\circ}C$ in a custom designed, evacuated reaction vessel. Here we introduce optimal procedure, reaction conditions, and samples/reactants size for calcium carbonate-phosphoric acid reaction and also provide the details for extracting, purifying and collecting $CO_2$ gas out of the reaction vessel. The measurements for ${\delta}^{18}O$ and ${\delta}^{13}C$ of $CO_2$ were performed at Seoul National University using a stable isotope ratio mass spectrometer (VG Isotech, SIRA Series II) operated in dual-inlet mode. The entire analysis precisions for ${\delta}^{18}O$ and ${\delta}^{13}C$ were evaluated based on the standard deviations of multiple measurements on 15 separate samples of purified $CO_2$. The pure $CO_2$ samples were taken from 100-mg aliquots of a solid calcium carbonate (Solenhofen-ori $CaCO_3$) during 8-day experimental period. The multiple measurements yielded the $1{\sigma}$ precisions of ${\pm}0.01$‰ for ${\delta}^{13}C$ and ${\pm}0.05$‰ for ${\delta}^{18}O$, comparable to the internal instrumental precisions of SIRA. Therefore, we conclude the method proposed in this study can serve as a way to produce an accurate secondary and/or laboratory $CO_2$ standard gas. We hope this study helps resolve difficulties in placing a laboratory working standard onto the international isotope scales and does make accurate comparisons with other data sets from other groups.