• Biomolecules & Therapeutics
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• 제14권3호
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• pp.171-177
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• 2006

Gastritis and gastric ulcer were known to be induced by gastic acid, stress, ethanol, Helicobacter pylori and free radical, etc. This study was performed for the development of a new drug or nutraceutical from medicinal plants or natural products with anti-gastritis, anti-ulcerative and gastroprotective activities. The water extract of Scutellaria baicalensis was exhibited potent inhibition in HCl ethanol-induced gastric lesion, acetic acid-induced and Shay ulcers, indicating the effects on gastric lesion and ulcer in rats. The water extract of Scutellaria baicalensis significantly inhibited HCl ethanol-induced gastric lesions at the oral dose of 300, 500 mg/kg. In pylorus ligated rats, the treatments of the water extract from Scutellaria baicalensis showed decrease in the volume of gastric secretion and acid output and increase pH at oral dose of 300, 500 mg/kg. And significantly reduced acetic acid-induced ulcer at the oral dose of 500 mg/kg for 12 days. In this study, we have found that the water extract from Scutellaria baicalensis had significant improvement in acute gastritis and ulcer at the dose of 300, 500 mg/kg and in chronic gastritis and ulcer at the dose of 500 mg/kg. Also we evaluated the antibacterial activity against Helicobacter pylori treated with Scutellaria baicalensis. Scutellaria baicalensis had a equivalent antibacterial activity with ampicilin against H. pylori at the dose of $100\;{\mu}/ml$. In histological examination, the water extract of Scutellaria baicalensis drastically restored gastric damages induced by HCl ethanol solution, pylorus- ligature and acetic acid. Therefore, we may use the water extract from Scutellaria baicalensis as antigastritic and antiulcerative agent for the purpose of the improvement or treatment of gastritis and gastric ulcer.

• 동의생리병리학회지
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• 제17권2호
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• pp.368-373
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• 2003

This study was conducted to evaluate the effects of Glycyrrhizae Radix water extract, known as depigmenting agent, on melanin biosynthesis in the HM3KO human melanoma cells. The inhibitory effect of Glycyrrhizae Radix water extract on melanogenesis was identified by mushroom tyrosinase assay in vitro. To determine whether Glycyrrhizae Radix water extract suppress melanin synthesis in cellular level, HM3KO cells were cultured in the presence of different concentrations of Glycyrrhizae Radix water extract and the effects on cell proliferation, melanin contents and tyrosinase activity were examined after 3 days. Treatment with Glycyrrhizae Radix at various concentrations did not exhibit any change of cell viability, and increased the cell proliferation. And the water extract of Glycyrrhizae Radix inhibited melanin contents and tyrosinase activity in a dose-dependent manner, compared with untreated group. These results suggest that the inhibitory effect of Glycyrrhizae Radix water extract on melanogenesis is due to the suppression of tyrosinase in HM3KO cells.

• 한국축산식품학회지
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• 제34권3호
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• pp.372-377
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• 2014

This study was conducted to evaluate the effects of oyster shell calcium powder (OSCP) as a substitute for phosphates in curing agent, on the quality of restructured pork ham. Restructured pork ham was processed under six treatment conditions: T1 (no additives), T2 (0.3% sodium tripolyphosphate), T3 (1.5% NaCl+0.5% whey protein), T4 (1.5% NaCl+0.5% whey protein+0.15% OSCP), T5 (1.5% NaCl+0.5% whey protein+0.3% OSCP), and T6 (1.5% NaCl+0.5% whey protein+0.5% OSCP). Addition of OSCP significantly increased the ash content and pH of restructured pork ham (p<0.05), but did not affect the cooking loss and water holding capacity values of restructured pork ham. Addition of OSCP had no effect on Hunter a and b surface color values of restructured pork ham, but did decrease the Hunter L surface color value (p<0.05). The addition of 0.5% OSCP showed significantly higher chewiness and springiness values of restructured pork ham, compared with the addition of phosphates (p<0.05). In conclusion, the addition of OSCP combined with low NaCl and 0.5% whey protein can be considered a viable substitute for phosphates in the curing agent, when processing restructured pork ham.

• 복식문화연구
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• 제19권1호
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• pp.96-103
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• 2011

It is well known that the Petasites japonicus has been used for a long time medicine for the treatment of allergic diseases such as lacquer poisoning. However, the exact components and dyeing properties of its effects is still not known. Therefore, the purpose of this study was to investigate the dyeing property and ultraviolet-cut ability of silk and nylon fabrics that was dyed variously with the Petasites japonicus. The Petasites japonicus extract was done by boiling with distilled water at $100^{\circ}C$ for 1 hour. As mordanting agent, we used Aluminum potassium sulfate ($AlK(SO_4)_2{\cdot}12H_2O$), Copper(II) sulfate pentahydrate ($CuSO_5{\cdot}5H_2O$), Iron(II)Chloride ($FeCl_2{\cdot}4H_2O$). The best K/S value of dyeing temperature and time, all the fabrics were $100^{\circ}C$, 90min. Silk fabric was dyed yellow(0.8Y 7.6/2.2) and nylon fabric was dyed reddish yellow(10.1 YR 7.4/3.0). Silk fabric and nylon fabric was changed greenish yellow on mordanting with $CuSO_5{\cdot}5H_2O$ and $FeCl_2{\cdot}4H_2O$ respectively. And the colorfastness of washing and dry-cleaning was improved by using mordanting agent(4~5 grade). Ultraviolet-cut ability(UV-B) was showed more 90% in dyed nylon fabrics.

• 한국식품저장유통학회지
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• 제25권1호
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• pp.98-106
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• 2018

본 연구에서는 쇠비름의 활용가치를 높이고 기능성 소재로서의 이용 가능성을 타진하기 위해 쇠비름 물 및 에탄올 추출물의 항산화와 항염증 활성을 탐색하였다. DPPH 라디칼 소거능과 FRAP을 이용한 총 항산화 효능 평가 결과 에탄올 추출물이 물 추출물보다 항산화 활성이 우수한 것을 확인하였고, ABTS 라디칼 소거능은 $1,000{\mu}g/mL$의 농도에서만 쇠비름 물 추출물의 활성이 더 높았다. 한편, 세포독성을 살펴보기 위하여 쇠비름 물 및 에탄올 추출물을 이용해 대식세포에 MTT assay를 수행한 결과, $4,000{\mu}g/mL$의 이하 농도까지는 세포의 생존율에 영향을 미치지 않는 것을 확인하였다. $1.0{\mu}g/mL$ LPS로 염증반응을 유도시킨 세포에 대해 쇠비름 물 추출물에 비해 에탄올 추출물이 더 효과적으로 농도의존적인 NO와 ROS 생성 억제 효과가 있었다. 추가적으로 세포의 항염증 효능의 지표 단백질로 알려진 TNF-${\alpha}$, IL-$1{\beta}$, IL-6의 발현을 확인하였을 때 LPS 단독 처리군 대비 쇠비름 에탄올 추출물에서 3가지 단백질 모두의 발현양이 감소하는 것을 확인하였다. 이상의 결과를 종합하여 볼 때 쇠비름 에탄올 추출물은 물 추출물에 비해 높은 항산화와 항염증 활성을 가지는 것으로 확인되었으며, 추후 쇠비름의 유효성분 추출을 통한 항염증 물질의 연구와 염증 억제 성분의 분리 및 그 작용기전 연구에 기초 자료가 될 것으로 사료된다.

• 한국구조물진단유지관리공학회 논문집
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• 제9권4호
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• pp.177-186
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• 2005

최근 콘크리트 구조물은 염해, 중성화 및 동결융해 등에 의한 내구성능 저하의 사례가 증가하고 있다. 이렇게 내구성능이 저하된 콘크리트에 대한 다양한 대책이 강구되고 있으며, 그 중에서도 콘크리트의 표면을 보호하여 내구성능 저하의 요인을 차단하는 표면처리공법이 많이 사용되고 있다. 그러나 에폭시 등 유기계 및 시멘트계 보수재는 콘크리트의 물성의 차이로 인하여 시간이 경과함에 따라 보수층의 파단 및 들뜸 등 문제가 발생하는 사례가 보고되고 있다. 저자들은 콘크리트의 물성과 동일한 무기계를 주성분으로 한 표면 성능 개선제를 콘크리트에 도포하면, 칼슘이온 등과 반응하여 콘크리트의 조직이 치밀해짐으로써 $CO_2$ 가스, 염소이온 및 물 등 내구성능 저하요인을 차단하는 보수재를 개발하고 있다. 본 연구에서는 개발된 표면성능 개선제의 침투성능, 수밀성능, 통기성능, 화학저항성능, 용출저항 성능 및 열화된 콘크리트에 대한 적용성에 대하여 평가하였다. 그 결과, 표면성능 개선제는 콘크리트의 내부로 10mm 이상 침투되며 콘크리트의 수밀성능 및 통기성능 등을 향상시키는 것으로 나타났다. 따라서, 본 연구에서 개발된 표면성능 개선제를 콘크리트 구조물에 도포하면 콘크리트의 내구성능 저하를 방지할 수 있을 것으로 판단된다.

• The Journal of Advanced Prosthodontics
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• 제10권5호
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• pp.374-380
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• 2018

PURPOSE. The effect of silica-based glass-ceramic liners on the tensile bond strength between zirconia and resin-based luting agent was evaluated and compared with the effect of 10-methacryloyloxydecyl dihydrogen phosphate (MDP)-containing primers. MATERIALS AND METHODS. Titanium abutments and zirconia crowns (n = 60) were fabricated, and the adhesive surfaces of the specimens were treated by airborne-particle abrasion. The specimens were divided into 5 groups based on surface treatment: a control group, 2 primer groups (MP: Monobond Plus; ZP: Z Prime Plus), and 2 liner groups (PL: P-containing Liner; PFL: P-free Liner). All specimens were cemented with self-adhesive resin-based luting agent. After 24-hour water storage and thermocycling (5,000 cycles, $5^{\circ}C/55^{\circ}C$), the tensile bond strength was measured using a universal testing machine. Failure mode analysis and elemental analysis on the bonding interface were performed. The data were analyzed using Kruskal-Wallis test, Dunn's post hoc test, and Fisher's exact test. RESULTS. The liner groups and primer groups showed significantly higher tensile bond strengths than that of the control group (P<.05). PFL showed a significantly higher tensile bond strength than the primer groups (P<.05). The percentage of mixed failure was higher in the primer groups than in the control group (P<.001), and all the specimens showed mixed failure in the liner groups (P<.001). A chemical reaction area was observed at the bonding interface between zirconia and liner. CONCLUSION. The application of liner significantly increased the tensile bond strength between zirconia and resin-based luting agent. PFL was more effective than MDP-containing primers in improving the tensile bond strength with the resin-based luting agent.

• 한국식품과학회지
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• 제34권5호
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• pp.805-810
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• 2002

본 연구는 키위연육제 개발의 기초자료로 제공하기 위해 단백분해효과가 있는 국내산 키위를 건조가공한 후 건조방법과 부형제 첨가에 따른 이화학적 특성을 비교분석하였고 12주 동안 저장하면서 품질변화를 관찰하였다. 건조 이전 전처리를 달리한 경우 paste 형태로 전처리를 한 경우가 dice 전처리 처리구보다 2.0배, 일반 과육형태보다는 1.3배 높은 단백불해활성을 나타내었다. 건조과정 중 변화를 보면 열풍 건조는 9시간 동안 동결건조는 46시간 동안 키위 paste의 수분감소가 90% 이상 진행되었으며 이 시간 동안 색의 변화가 주로 관찰되었다. 건조분말은 열풍건조한 경우 동결건조 처리구에 비해 수율이 낮았으나 수분함량은 거의 차이가 나지 않았다. 열풍건조한 처리구의 경우 현저한 단백분해효소 활성의 저하를 나타내었고 동결건조한 처리구는 열풍건조보다 약 6.6배정도 많은 단백분해효과를 나타내었다. 키위 고유의 색은 동결건조를 하는 경우 유지되었다. 건조전 부형제를 첨가한 것에 의한 효과를 보면 동결건조에서는 부형제에 의한 차이가 적으나 열풍건조에서는 dextrin 5%첨가한 키위분말이 높은 건조분말수율과 낮은 수분함량을 보여주었다. 단백분해활성은 부형제를 넣고 열풍건조한 처리구의 경우 부형제 무처리구보다 약 $3.2{\sim}3.6$배 더 높은 효소역가를 보여주었고 이 역가는 동결건조 결과물에 비해 60% 정도 잔존하는 것으로 나타났다. 부형제에 의한 색의 차이는 관찰되지 않았다. 12주 저장하는 동안 모든 처리구에서 건조 전 부형제를 첨가한 분말연육체의 수분함량은 저장 내내 안정된 경향의 수분보유력을 보여주었다. 단백분해효소 활성은 열풍건조의 경우 건조전 부형제를 첨가한 처리구는 저장 4주까지 단백분해효소의 활성 감소를 보이다가 안정화되었다. 따라서 키위 고유의 색을 유지하고 가공 후 분말내 단백분해효소 잔존량을 높이기 위해서는 동결건조가 효율적이나 부형제를 적절히 처리한 후 열풍건조를 하는 방법도 경제성 측면에서 좋은 대안이 될 수 있음을 보여주었다.

• 대한본초학회지
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• 제34권1호
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• pp.51-57
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• 2019

Objectives : Nypa fruticans Wurmb. (NF) have been used as a traditional medicine to treat inflammatory diseases in East-South Asia. However, it is largely undiscovered whether NF water extract could exhibit anti-inflammatory activities against tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$)-induced inflammatory responses on human keratinocytes, HaCaT cells. Therefore, this study was aimed to investigate the anti-inflammatory activity of NF water extract on TNF-${\alpha}$-induced inflammatory responses in HaCaT cells. Methods : To investigate the anti-inflammatory activites of NF water extract in HaCaT cells, the inflammatory model of HaCaT cells was established under a suitable concentration (10 ng/ml) of human TNF-${\alpha}$ (hTNF-${\alpha}$). HaCaT keratinocyte cells were pre-treated with NF water extract for 1 h, and then stimulated with hTNF-${\alpha}$. Then, the cells were harvested to measure the inflammatory mediators such as inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2 and prostaglandin $E_2$ ($PGE_2$), and pro-inflammatory cytokine including TNF-${\alpha}$ and interleukin (IL)-6. In addition, we examined the inhibitory mechanisms of NF, mitogen activated protein kinases (MAPKs) and inhibitory kappa B alpha ($I{\kappa}-B{\alpha}$) Results : The treatment of NF inhibited the hTNF-${\alpha}$-induced elevation of iNOS, COX-2, and $PGE_2$ in HaCaT cells. In addition, NF treatment inhibited the hTNF-${\alpha}$-induced elevation of TNF-${\alpha}$ and IL-6. Furthermore, NF treatment inhibited the activation of MAPKs but not degradation of $I{\kappa}-B{\alpha}$. Conclusions : Taken together, our result suggest that treatment of NF could inhibit the hTNF-${\alpha}$-induced inflammatory responses via deactivation of MAPKs in HaCaT cells. This study could suggest that NF could be a beneficial agent to prevent skin damage or inflammation.

• 동의생리병리학회지
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• 제17권1호
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• pp.213-219
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• 2003

We investigated the effects of Insamsapye-tang (ISSPT) water extract on the cell proliferation of human lung carcinoma A549 cells. ISSPT treatment resulted in the inhibition of cell proliferation in a concentration-dependent manner. This anti-proliferative effect of A549 cells by ISSSPT treatment was associated with morphological changes such as membrane shrinking and cell rounding up. DNA flow cytometric histograms showed that population of G1 phase of the cell cycle was increased by ISSPT treatment in a concentration-dependent manner. ISSPT treatment induced the levels of tumor suppressor p53 protein and cyclin-dependent kinase (Cdk) inhibitor p27 without significant alteration of cyclins and Cdks expression. In addition, ISSPT treatment resulted in down-regulation of phosphorylated retinoblastoma protein (pRB). However, the levels of p130, the pRB family protein, and transcription factors. E2F-1 and E2F-4. were remained unchanged. The present results indicated that ISSPT-induced inhibition of lung cancer cell proliferation is associated with the blockage of G1/S progression and the induction of apoptosis, and we suggest that ISSPT will be an effective therapeutic agent on human lung cancer.