• International Journal of Industrial Entomology and Biomaterials
• /
• 제41권2호
• /
• pp.36-44
• /
• 2020

Mulberry root bark is one of potential plant sources for antioxidant materials which can be used for the relief of oxidative stress. To explore the effects of solvent type and temperature on the structural characteristics and antioxidant activity of the root bark extracts, we prepared various extracts of mulberry root bark (Morus alba L.) using 0 - 100 % ethanol (EtOH) at RT - 100℃. EtOH concentration and temperature critically affected the extraction yields, the content of bioactive components, and antioxidant activity of the extracts. Use of high content of EtOH solvent and low temperature resulted in the low extraction yield. Meanwhile, it was revealed that the extract prepared using absolute EtOH at room temperature contained polyphenols and flavonoids with the highest contents among other extracts. Interestingly, the temperature differently affected the polyphenol and flavonoid contents according to the solvent types. In the case of 30% EtOH solvent, polyphenol and flavonoid contents increased with an increase in temperature, whereas in the case of 70 and 100 % EtOH, these contents decreased. Using the radical scavenging assay, it was confirmed that the 100% EtOH extracts had higher antioxidant activity compared to distilled water (DW) extracts regardless of temperature. Also, heating might extract more antioxidant components from the root bark. Especially, the extract prepared using 30% EtOH solvent at 100℃ showed the highest antioxidant activity. Taken together, these experimental results imply that the extraction parameters should be designed carefully considering the productivity, the extracted bioactive components, and antioxidant activity.

• 한국환경과학회지
• /
• 제11권11호
• /
• pp.1173-1181
• /
• 2002

The present study was undertaken to investigate the effect of low temperature and salicylic acid(SA) on the chilling tolerance of acclimated and nonacclimated cucumber(Cucurmis sativus L.) seedlings. The acclimation phenomenon was characterized in chilling-sensitive cucumber seedlings and found to have a significant effect on the survival and shoot dry weights. The injuries experienced by the acclimated seedlings in the third leaf stage were on average smaller by half than those experienced by the nonacclimated seedlings. Chilling also caused a large increase in the free proline levels, regardless of the acclimation status. Exogenous treatment with SA(0.5mM) resulted in improved growth and survival of the nonacclimated chilled seedlings, indicating that SA induced chilling tolerance and SA and acclimation had common effects. The application of cycloheximide in the presence of SA restored the acclimation-induced chilling tolerance. The elevated proline level observed in the cold-treated and SA-treated plants was more pronounced in the light than in the dark at a chilled temperature, indicating that endogenous proline may play a role in chilling tolerance by stabilizing the water status in response to chilling. From these results it is suggested that SA provided protection against low-temperature stress by increasing the proline accumulation, and pre-treatment with SA may induce antioxidant enzymes leading to increased chilling tolerance.

• 한국세라믹학회지
• /
• 제34권10호
• /
• pp.1021-1026
• /
• 1997

RuO2 thin film has reasonably good conductivity and stiffness and it is thought to substitute for the cantilever beam made up of Pt and Si3N4 double layers in microactuators. Therefore, anisotopic Si etching was performed using RuO2 thin film as a mask layer in 25 wt. % TMAH water solution. In the etching temperature ranging from 6$0^{\circ}C$ to 75$^{\circ}C$, the etch rates of all the crystallographic directions increased linearly as the etching temperature increased. The etch rate ratio(selectivity) of [111]/[100] which varied from 0.08 to 0.14, was not sensitive to temperature. The activation energies for [110] direction, [100] direction and [111] direction were 0.50, 0.66 and 1.04eV, respectively. RuO2 cantilever beam with a clean surface was formed at the etching temperatures of 6$0^{\circ}C$ and $65^{\circ}C$. But the damages due to formation of pin holes on RuO2 surface were observed beyond 7$0^{\circ}C$. The tensile stress of RuO2 thin films caused the cantilever bending upward. As a result, it was demonstrated that the formation of conducting oxide RuO2 cantilever beam which can replace the role of an electrode and supporting layer could be possible by TMAH solution.

• Safety and Health at Work
• /
• 제8권4호
• /
• pp.327-328
• /
• 2017

Despite provision of drinking water as the most common method of occupational heat stress prevention, there remains confusion in hydration messaging to workers. During work site interactions in a hot and humid climate, workers commonly report being informed to consume tepid fluids to accelerate rehydration. When questioned on the evidence supporting such advice, workers typically cite that fluid absorption is delayed by ingestion of chilled beverages. Presumably, delayed absorption would be a product of fluid delivery from the gut to the intestines, otherwise known as gastric emptying. Regulation of gastric emptying is multifactorial, with gastric volume and beverage energy density the primary factors. If gastric emptying is temperature dependent, the impact of cooling is modest in both magnitude and duration (${\leq}5$ minutes) due to the warming of fluids upon ingestion, particularly where workers have elevated core temperature. Given that chilled beverages are most preferred by workers, and result in greater consumption than warm fluids during and following physical activity, the resultant increased consumption of chilled fluids would promote gastric emptying through superior gastric volume. Hence, advising workers to avoid cool/cold fluids during rehydration appears to be a misinterpretation of the research. More appropriate messaging to workers would include the thermal benefits of cool/cold fluid consumption in hot and humid conditions, thereby promoting autonomy to trial chilled beverages and determine personal preference. In doing so, temperature-based palatability would be maximized and increase the likelihood of workers maintaining or restoring hydration status during and after their work shift.

• Journal of Microbiology and Biotechnology
• /
• 제17권4호
• /
• pp.638-643
• /
• 2007

The full encoding sequence for human type II hexokinase (HXK II) was cloned into the E. coli expression vector pET 21b and expressed as a C-terminally hexahistidine-tagged protein in the BL2l (DE3) strain. The IPTG-induced HXK II approximately accounted for 17% of the total E. coli proteins, and 81% of HXK $II_{6{\times}His}$ existed in inclusion bodies. To improve the production of soluble recombinant HXK II protein, in the functionally active form, we used low temperature, and the osmotic stress expression method. When expressed at $18^{\circ}C$, about 83% of HXK $II_{6{\times}His}$ existed in the soluble fraction, which amounted to a 4.1-fold yield over that expressed at $37^{\circ}C$. The soluble form of HXK $II_{6{\times}His}$ was also highly produced in the presence of 1M sorbitol under the standard condition $(37^{\circ}C)$, which indicated that temperature downshift and low water potentials were required to improve the yield of active recombinant HXK II protein. The expressed protein was purified by metal chelate affinity chromatography performed in an IDA Excellose column charged with $Ni^{2+}$ ions, resulting in about 40mg recombinant HXK II protein obtained with purity over 89% from 51 of E. coli culture. The identity of HXK $II_{6{\times}His}$ was confirmed by Western blotting analysis. Taken together, using the stress-governed expression described in this study, human active HXK II can be purified in sufficient amounts for biochemical and biomedical studies.

• 터널과지하공간
• /
• 제25권2호
• /
• pp.168-185
• /
• 2015

본 연구에서는 천부의 암반 공동에 대용량 고온의 열에너지를 저장하는 경우 주변 암반에 야기되는 열-수리-역학적 연계거동을 살펴보고, 이에 지하수위와 암반 투수계수 등 수리적 조건이 미치는 영향을 검토하였다. 해석대상을 투수계수가 비교적 낮은 수준($10^{-17}m^2$)인 결정질 암반으로 가정할 때 열에너지 저장으로 인한 암반 거동에 지하수가 미치는 영향은 크지 않을 것으로 예측되었다. 저장 공동이 지하수위 하부에 위치하는 경우의 온도, 주응력, 변위 분포 등은 저장공동이 불포화대에 위치하는 경우와 거의 동일하게 나타났다. 암반내 열전달 특성은 암반의 투수계수에 매우 큰 영향을 받는 것으로 나타났다. 암반의 투수계수를 $10^{-15}m^2$ 이하로 가정한 경우 열전달은 주로 암반에 전도에 의한 것으로 판단할 수 있었으나, 투수계수를 $10^{-12}m^2$으로 가정하는 경우 지하수 대류에 의한 상향 열유동이 뚜렷이 관찰되었다. 암반 투수계수의 크기에 따라 열수의 대류나 비등으로 인한 상변화 등 복합적인 유동 특성을 나타났으며, 온도, 압력, 포화도 분포가 상이하게 발달하였다.

• 한국수자원학회논문집
• /
• 제56권12호
• /
• pp.993-1003
• /
• 2023

대기온실가스 증가로 전지구 평균기온은 산업화 이전 대비 1.1℃ 상승했고 수자원시스템의 공급능력에 상당한 변화가 예상된다. 본 연구에서는 금강수계 내 여러 중권역의 이수안전도와 기후조건의 관계(기후반응함수)를 단변량 함수로 나타내 기후민감도를 동시에 평가할 수 있는 방법을 제안하였다. 사례연구를 위해 GR6J 모형으로 중권역별 자연유출을 모의했고 이를 Water Evaluation And Planning (WEAP) 최적모형에 입력해 2030년 수요전망에 대한 공급신뢰도를 평가하였다. 여러 중권역의 이수안전도를 동시에 비교하기 위해 평균 강수량과 잠재증발산량의 비율을 독립변수 사용하여 단변량 기후민감도 함수를 개발하였다. 사례연구 결과, 1991-2020 자연유출을 이용해 수계전체 물부족을 최소화시키는 운영을 가정했을 때 공급신뢰도는 19개 중권역 중 보청천유역에서 가장 낮았다. 하천유지유량의 우선순위를 농업용수와 생공용수과 동일하게 조정한 시나리오에서는 보청천유역, 초강유역, 논산천유역의 이수안전도가 크게 감소하는 것으로 나타났다. 보청천유역, 초강유역, 논산천유역의 이수안전도는 모든 기후스트레스 테스테에서 크게 감소한 반면, 미호강유역, 금강공주유역, 금강하구유역은 아주 건조한 기후조건에서만 이수안전도가 감소했다. 대규모 인프라에서의 공급이 원활한 중권역의 기후민감도는 크게 변하지 않았다. 2021-2050 기후전망을 민감도함수에 적용했을 때 금강수계의 공급신뢰도는 대체로 좋아질 가능성이 높지만 하천유지유량 우선순위를 높이게 되면 지형적, 인위적으로 고립된 중권역에서 물부족은 심해질 것으로 분석되었다. 2021-2050기간 금강수계의 이수안전도는 기후스트레스 보다 하천관리정책의 변화에 더 큰 영향을 받을 것으로 판단된다.

• 한국수자원학회:학술대회논문집
• /
• 한국수자원학회 2002년도 학술발표회 논문집(I)
• /
• pp.3-16
• /
• 2002

This article describes some of the recent and on-going research developments of the author at Colorado State University. Advances in the field of sedimentation and river mechanics include basic research and computer modeling on several topics. Only a few selected topics are considered here: (1) analytical determination of velocity profiles, shear stress and sediment concentration profiles in smooth open channels; (2) experiments on bedload particle velocity in smooth and rough channels; (3) field measurements of sediment transport by size fractions in curved flumes. In terms of computer modeling, significant advances have been achieved in: (1) flashflood simulation with raster-based GIOS and radar precipitation data; and (2) physically-based computer modeling of sediment transport at the watershed scale with CASC2D-SED. Field applications, measurements and analysis of hydraulic geometry and sediment transport has been applied to: (1) gravel-bed transport measurements in a cobble-bed stream at Little Granite Creek, Wyoming; (2) sand and gravel transport by size fraction in the sharp meander bends of Fall River, Colorado; (3) changes in sand dune geometry and resistance to flow during major floods of the Rhine River in the Netherlands; (4) changes in hydraulic geometry of the Rio Grande downstream of Cochiti Dam, New Mexico; and (5) analysis of the influence of water temperature and the Coriolis force on flow velocity and sediment transport of the Lower Mississippi River in Louisiana. Recent developments also include two textbooks on "Erosion and Sedimentation" and "River Mechanics" by the author and state-of-the-art papers in the ASCE Journal of Hydraulic Engineering.

• 에너지공학
• /
• 제27권1호
• /
• pp.65-75
• /
• 2018

The emission of greenhouse gases mainly carbon dioxide and methane is the result of rapid industrialization to meet the demands of ever-growing population. This has resulted in an increase of global temperature which in turn is responsible for severe environmental, social, ecological and economic losses, commonly known as to as 'climate change'. This study attempts to highlight the impacts of climate change mainly focussing on water contamination, sanitation and open defecation in India. The requirement for the instantaneous employment of environment friendly technologies along with improved sanitary system has been discussed. Various other issues which are also linked to climate change that need further management like managing water resources, deterioration in human health, economic losses, modification and successful implementation of policies have been pointed out. Furthermore, stress has been made for the urgent adaptation and rethinking for making strategies along with the involvement of women in order to cop up challenges offered by climate change.

• 한국환경과학회지
• /
• 제15권10호
• /
• pp.961-966
• /
• 2006

An algal assay procedure using an indigenous phytoplankton assemblage was tested to estimate the propagation of red tide phytoplankton species and determine the optimal time interval at which to measure growth yield in eutrophic marine waters where red tides frequently occur. Various red tide phytoplankton species were propagated on a large scale by adding nitrogen or phosphorous. This procedure was useful for estimating the limiting nutrient, elucidating the mechanisms underlying red tides, and determining the levels of increases in organic matter in eutrophic coastal waters. The algal assay using indigenous C. polykrikoides showed that this species did not always propagate, apparently because of very low concentrations of trigger elements that are necessary for its growth, rather than as a result of other environmental characteristics, e.g., water temperature or stress from sampling. In the winter, when water temperatures are lower than in spring, summer, or autumn, maximum propagation and the limiting nutrient could be estimated by measuring phytoplankton biomass at 2 - 3-day intervals. However, in the other seasons, when water temperatures are higher, phytoplankton biomass should be measured at 2-day intervals. In particular, daily monitoring will be required to determine precise growth yields in warm seasons.