• Title/Summary/Keyword: Water temperature stress

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Identification of genes related to ER stress in bZIP28 gene transgenic potato plant

  • Kim, Dool Yi;Kim, Kyung Hwa;Choi, Man Soo;Ok, Hyun Choog;Kim, Jae Hyun
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.153-153
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    • 2017
  • Potato (Solanum tubersosum L.) is susceptible to various environmental stresses such as frost, high temperature, and drought. Enhancement of potato drought tolerance can reduce yield loss under drought that has negative effect on potato tuber growth. Genetic engineering can be utilized to achieve this goal, but such approaches using endogenous potato genes have rarely been applied. Since unpredictable global weather changes cause more severe and frequent water limiting conditions, improvement of potato drought tolerance can minimize such adverse effects under drought and can impact on sustainable potato production. Genetic engineering can be utilized to improve potato drought tolerance, but such approaches using endogenous potato genes have rarely been applied. We were obtained AtbZIP28 gene transgenic potato plants. It is identified transcript levels at various stress conditions, polyethylene glycol (PEG), NaCl, abscisic ${\underline{acid}}$ (ABA). Also, For identification to regulate ER stress response genes in AtbZIP28 gene transgenic potato plant, we screened seven potato genes from RNA-seq analysis under TM treatment. Five and two genes were up- and down-regulated by TM, respectively. Their expression patterns were re-examined at stress agents known to elicit TM, DTT, DMSO and salt stress.

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Protective effect of soursop (Annona muricata linn.) juice on oxidative stress in heat stressed rabbits

  • Jimoh, Olatunji Abubakar;Ayedun, Eyanlola Soladoye;Oyelade, Waheed Abimbola;Oloruntola, Olugbenga David;Daramola, Olajumoke Temidayo;Ayodele, Simeon Olugbemiga;Omoniyi, Idowu Samuel
    • Journal of Animal Science and Technology
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    • v.60 no.11
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    • pp.28.1-28.6
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    • 2018
  • Background: Preventing oxidative stress in heat stressed animals may be possible by increasing antioxidant defence via exogenous administration of antioxidant substrate and/or its precursors. The study aimed to investigate the effect of Soursop juice in mitigating oxidative stress induced by heat stress in rabbit. Methods: Sixty mixed breed rabbit bucks aged 12-18 months old with the average weight of $1826{\pm}8.35$ g/rabbit, randomly allotted to experimental treatments of four replicates each, in a completely randomized design during high-temperature humidity index in Ado Ekiti, Southwest Nigeria. Soursop juice (SSJ) was administered via oral drenched daily per kg body weight (BW), to designated treatment 1 to 5; $0.55mlkg^{-1}BW$ distilled water (control), $0.55mlkg^{-1}BW$ SSJ, $1.11mlkg^{-1}BW$ SSJ, $1.67mlkg^{-1}BW$ SSJ and $2.22mlkg^{-1}BW$ SSJ, respectively. Fastened blood samples were collected at days 28 and 56, and assay for serum protein, cholesterol, triglycerides, superoxide dismutase, catalase, reduced glutathione and lipid peroxidation using standard procedures. Result: Result revealed that SSJ demonstrated hypocholesterolemic effect in a dose-dependent manner throughout the study. Effect of chronic administration of SSJ to heat stressed rabbits proved beneficial, as SSJ reduced serum lipid peroxidation and enhanced antioxidant activity over 8 weeks. Conclusion: Administration of soursop juice to heat-stressed bucks at $2.22mlkg^{-1}BW$ offered optimum antioxidant defense against oxidative stress.

Bacterial Exopolysaccharides: Insight into Their Role in Plant Abiotic Stress Tolerance

  • Bhagat, Neeta;Raghav, Meenu;Dubey, Sonali;Bedi, Namita
    • Journal of Microbiology and Biotechnology
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    • v.31 no.8
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    • pp.1045-1059
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    • 2021
  • Various abiotic stressors like drought, salinity, temperature, and heavy metals are major environmental stresses that affect agricultural productivity and crop yields all over the world. Continuous changes in climatic conditions put selective pressure on the microbial ecosystem to produce exopolysaccharides. Apart from soil aggregation, exopolysaccharide (EPS) production also helps in increasing water permeability, nutrient uptake by roots, soil stability, soil fertility, plant biomass, chlorophyll content, root and shoot length, and surface area of leaves while also helping maintain metabolic and physiological activities during drought stress. EPS-producing microbes can impart salt tolerance to plants by binding to sodium ions in the soil and preventing these ions from reaching the stem, thereby decreasing sodium absorption from the soil and increasing nutrient uptake by the roots. Biofilm formation in high-salinity soils increases cell viability, enhances soil fertility, and promotes plant growth and development. The third environmental stressor is presence of heavy metals in the soil due to improper industrial waste disposal practices that are toxic for plants. EPS production by soil bacteria can result in the biomineralization of metal ions, thereby imparting metal stress tolerance to plants. Finally, high temperatures can also affect agricultural productivity by decreasing plant metabolism, seedling growth, and seed germination. The present review discusses the role of exopolysaccharide-producing plant growth-promoting bacteria in modulating plant growth and development in plants and alleviating extreme abiotic stress condition. The review suggests exploring the potential of EPS-producing bacteria for multiple abiotic stress management strategies.

Numerical Analysis of Coupled Thermo-Hydro-Mechanical (THM) Behavior at Korean Reference Disposal System (KRS) Using TOUGH2-MP/FLAC3D Simulator (TOUGH2-MP/FLAC3D를 이용한 한국형 기준 처분시스템에서의 열-수리-역학적 복합거동 특성 평가)

  • Lee, Changsoo;Cho, Won-Jin;Lee, Jaewon;Kim, Geon Young
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.17 no.2
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    • pp.183-202
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    • 2019
  • For design and performance assessment of a high-level radioactive waste (HLW) disposal system, it is necessary to understand the characteristics of coupled thermo-hydro-mechanical (THM) behavior. However, in previous studies for the Korean Reference HLW Disposal System (KRS), thermal analysis was performed to determine the spacing of disposal tunnels and interval of disposition holes without consideration of the coupled THM behavior. Therefore, in this study, TOUGH2-MP/FLAC3D is used to conduct THM modeling for performance assessment of the Korean Reference HLW Disposal System (KRS). The peak temperature remains below the temperature limit of $100^{\circ}C$ for the whole period. A rapid rise of temperature caused by decay heat occurs in the early years, and then temperature begins to decrease as decay heat from the waste decreases. The peak temperature at the bentonite buffer is around $96.2^{\circ}C$ after about 3 years, and peak temperature at the rockmass is $68.2^{\circ}C$ after about 17 years. Saturation of the bentonite block near the canister decreases in the early stage, because water evaporation occurs owing to temperature increase. Then, saturation of the bentonite buffer and backfill increases because of water intake from the rockmass, and bentonite buffer and backfill are fully saturated after about 266 years. The stress is calculated to investigate the effect of thermal stress and swelling pressure on the mechanical behavior of the rockmass. The calculated stress is compared to a spalling criterion and the Mohr-Coulumb criterion for investigation of potential failure. The stress at the rockmass remains below the spalling strength and Mohr-Coulumb criterion for the whole period. The methodology of using the TOUGH2-MP/FLAC3D simulator can be applied to predict the long-term behavior of the KRS under various conditions; these methods will be useful for the design and performance assessment of alternative concepts such as multi-layer and multi-canister concepts for geological spent fuel repositories.

Dynamically Induced Anomalies of the Japan/East Sea Surface Temperature

  • Trusenkova, Olga;Lobanov, Vyacheslav;Kaplunenko, Dmitry
    • Ocean and Polar Research
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    • v.31 no.1
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    • pp.11-29
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    • 2009
  • Variability of sea surface temperature (SST) in the Japan/East Sea (JES) was studied using complex empirical orthogonal function (CEOF) analysis. Two daily data sets were analyzed: (1) New Generation 0.05o-gridded SST from Tohoku University, Japan (July 2002-July 2006), and (2) 0.25o-gridded SST from the Japan Meteorological Agency (October 1993-November 2006). Linkages with wind stress curl were revealed using 6-h 1o-gridded surface zonal and meridional winds from ancillary data of the Sea- WiFS Project, a special National Centers for Environmental Prediction/National Center for Atmospheric Research (NCEP/NCAR) product (1998-2005). SST anomalies (SSTA) were obtained by removing the seasonal signal, estimated as the leading mode of the CEOF decomposition of the original SST. Leading CEOF modes of residual SSTA obtained from both data sets were consistent with each other and were characterized by annual, semiannual, and quasi-biennial time scales estimated with 95% statistical significance. The Semiannual Mode lagged 2 months behind the increased occurrence of the anticyclonic (AC) wind stress curl over the JES. Links to dynamic processes were investigated by numerical simulations using an oceanic model. The suggested dynamic forcings of SSTA are the inflow of subtropical water into the JES through the Korea Strait, divergence in the surface layer induced by Ekman suction, meridional shifts of the Subarctic Front in the western JES, AC eddy formation, and wind-driven strengthening/weakening of large-scale currents. Events of west-east SSTA movement were identified in July-September. The SSTA moved from the northeastern JES towards the continental coast along the path of the westward branch of the Tsushima Current at a speed consistent with the advective scale.

Effects of supplemental different clay minerals in broiler chickens under cyclic heat stress

  • Jaewoo An;Jihwan Lee;Minho Song;Hanjin Oh;Yongju Kim;Seyeon Chang;Youngbin Go;Dongcheol Song;Hyunah Cho;Haeryoung Park;Hyeun Bum Kim;Jinho Cho
    • Journal of Animal Science and Technology
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    • v.65 no.1
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    • pp.113-131
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    • 2023
  • The objective of this study was to investigate the effect of supplementing clay minerals and organic chromium in feed on broiler chicken under heat stress (HS). A total of 90 one-day-old broiler chicken (Arbor Acres) with an initial body weight of 45.0 ± 0.2 g were assigned to five treatment groups (six replications, three birds each cage): 1) NC group, basal diet under room temperature environment; 2) PC group, basal diet under high temperature (HT) environment; 3) ILT group, basal diet + 1% illite + HT; 4) ZLT group, basal diet + 1% zeolite + HT; 5) OC group, basal diet + 400 ppb/kg organic chromium + HT. The ILT and ZLT groups had significantly higher body weight than the PC group in 4 weeks. Apparent total tract digestibility of gross energy was increased in the ILT, ZLT, and OC groups compared to the PC group. The NC group had lower foot-pad dermatitis score than other groups. Escherichia coli population in the cecum and feces was decreased in the ZLT group than in the PC group. Lactobacillus in cecum and feces was significantly increased in the ZLT group than in the PC group. Regarding blood profiles, blood cortisol was decreased in the NC and ILT groups compared to the PC group. Water holding capacity and pH were increased in the ZLT group than the PC group. In conclusion, according to the results of growth performance, nutrients digestibility, bacteria counts, and meat characteristics, supplementation of the ZLT in broiler diet can alleviate HS.

Relation Between Water Content Ratio and Fire Performance of Class 1 Structural Light Weight Aggregate Concrete (1종 경량골재콘크리트의 함수율과 내화특성)

  • Song, Hun
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.2 no.4
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    • pp.321-327
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    • 2014
  • Structural light weight aggregate concrete are made with both coarse and fine light weight aggregates, but it is common with the high strength concrete to replace all or part with normal weight sand be called class 1 structural light weight aggregate concrete. Fire resistance of structural light weight aggregate concrete are determined by properties of high water content ratio and explosive spalling. Especially, structural light weight aggregate concrete is occurred serious fire performance deterioration by explosive spalling stem from thermal stress and water vapor pressure. This study is concerned with experimentally investigating fire resistance of class 1 structural light weight concrete. From the test result, class 1 structural light weight concrete is happened explosive spalling. The decrease of cross section caused by explosive spalling made sharp increasing gradient of inner temperature.

An Experimental Study on the Galling Characteristics of valve Seat Materials for Water Works (밸브시트 재료의 갤링 특성에 관한 실험적 연구)

  • 박성준;김영태;이상조
    • Journal of the Korean Society for Precision Engineering
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    • v.21 no.3
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    • pp.100-108
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    • 2004
  • Contamination of environment induces the shortage of drinkables. In this trend, the leakage of water that occurs by breakage or erosion of rubber valve seats is serious problem. Rubber is apt to cause breakage between two materials when they contact with each other. The possible way to avoid leakage of water by damage and breakdown of rubber is to replace that with metal. Because of this reason, nowadays, rubber is being substituted with metal as valve seat materials for water works. In tribology, a severe from of wear is characterized by local, macroscopic material transfer, removal, or formation of surface protrusions when two solid surfaces experience relative sliding under load. One of the major problems in sliding of metals is galling due to bad surface quality. Experimentally, there are various elements which influence on incipient galling, such as hardness, surface roughness, temperature, load, velocity and external environments. This paper is aimed at verifying the galling tendencies according to hardness, surface roughness, load and velocity and showing how much effect the factors have on the galling tendencies.

A study on the fire resistance method using FR-ECC in long tunnel (고인성내화모르터(FR-ECC)를 사용한 장대터널 내화안전대책에 관한 연구)

  • Kim, Se-Jong;Kim, Dong-Jun;Kwon, Young-Jin
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.13 no.1
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    • pp.9-18
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    • 2011
  • The spalling phenomenon occurs in high-strength concrete when several factors such as sharp temperature increase, high water content, low water/cement ratio and local stress concentration in material combine in the concrete material. On the basis of the factors, the preventing methods from the spalling are known as reduction of temperature increase, preventing of concrete fragmentation and fast drying of internal moisture. In this study, the reduction of temperature increase was proposed as the most effective spalling-preventing method among the spalling-preventing methods. Engineered cementitious composite for fireproof and repair materials was developed in order to protect the new and existing RC structures form exterior deterioration factors such as fire, cloride ion, etc. This study was carried out to estimate the fire-resisting performance of high strength concrete slab or tunnel lining by repaired engineered cementitious composite (ECC) or fiber reinforcement cemetitious composite (FRCC) under fire temperature curve. and them we will descrike the result of HIDA tunnel in Japan.

Evaluation of the Mechanical Characteristics of Frozen Sand, Considering Temperature and Confining Pressure Effects, in a Cryogenic Triaxial Compression Test (동결 삼축압축시험을 통한 동결 사질토의 온도 및 구속압력에 따른 역학적 특성 평가)

  • Park, Sangyeong;Jung, Sanghoon;Hwang, Chaemin;Choi, Hangseok
    • Journal of the Korean Geotechnical Society
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    • v.38 no.8
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    • pp.7-15
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    • 2022
  • Most studies have conducted cryogenic triaxial compression tests with frozen specimens prepared in a separate mold by one-directional freezing. This method has the potential to generate residual stress in a frozen specimen and cannot be adopted to simulate the application of the artificial ground freezing method in the field. Therefore, in this study, novel equipment and procedure for the cryogenic triaxial compression test were proposed to overcome the limitations of existing test methods. Therefore, the mechanical characteristics of frozen sand, considering the effect of temperature and confining pressure, were evaluated. As the freezing temperature decreased, the brittleness of frozen sand increased, and the strength increased due to a decrease in the unfrozen water content and an increase in the ice strength. A higher confining pressure resulted in an increase in interparticle friction and the pressure melting phenomenon, which caused strength reduction. Thus, it was found that the mechanical behaviors of frozen sand were simultaneously affected by both temperature and confining pressure.