Park Chul Soo;Kim Yang-Kil;Han Ouk-Kyu;Lee Mi Ja;Park Jong-Chul;Seo Jae-Hwan;Hwang Jong-Jin;Kim Jung-Gon;Kim Tae Wan
KOREAN JOURNAL OF CROP SCIENCE
/
v.50
no.5
/
pp.346-355
/
2005
To investigate the application of biochemical markers' and small-sample methods using whole-wheat flours for screening in early generation in Korean wheat breeding system, 74 Korean wheats, including cultivars, local breeding lines and experimental lines, were analyzed. Seed storage protein and amylose contents of grains were evaluated. Biochemical makers, including granule bound starch synthase (GBSS), high molecular weigh glutenin subunits (HMW-GS) and friabilin were also evaluated by using one-dimensional sodium dodecyl sulfate-polyacryla-mide gel electrophoresis with a single kernel. The smallsample methods, including modified SDS-sedimentation test (MST), micro-alkaline water retention capacity (AWRC) and whole-wheat flour swelling volume (WSV) were also tested in this study. Protein content, MST and AWRC was $11.0 - 15.8\%$, 2.7 - 26.2 ml and $71.9 - 109.7\%$, respectively. Apparent and total amylose content and WSV was $20.6 - 25.0\%$, $26.1 - 32.4\%$ and 9.0 - 16.9 ml, respectively. There were highly significant correlations between MST and AWRC (r=0.592, P<0.001), but Korean wheats showed no significant difference in protein content, amylose content and small-sample methods. In the biochemical markers, Korean wheats contained all three GBSS encoded by Wx loci, except for Suwon 252. Korean wheats showed the high frequency ($58.1\%$) of 1Dx2.2 + 1Dy12 subunits of HMW-GS. Friabilin band was present in 46 lines ($62.2\%$) and absent in 28 lines ($37.8\%$). Friabilin-absence lines showed the higher MST (14.9 ml) and AWRC ($92.1\%$) value than friabilin-presence lines (8.5 ml and $82.4\%$, respectively).
Fermentation characteristics, nutrient retention and aerobic stability of barley silages prepared using 6 commercial inoculants were evaluated using 126 mini-silos (3-L) in a completely randomized design. Whole barley forage was chopped, wilted to 39% DM and treated with water (control, S) or one of six inoculants: A (containing Lactobacillus plantarum); B (L. plantarum and Enterococcus faecium); C (L. plantarum and Pediococcus cerevisiae); D (L. plantarum, Pediococcus pentosaceus and Propionibacterium freudenreichii, plus hydrolytic enzymes); E (Lactobacillus buchneri plus hydrolytic enzymes); F (L. buchneri and P. pentosaceus plus hydrolytic enzymes). Samples of treated forage were collected for analysis at the time of ensiling, and then 18 silos of each treatment were filled, capped and weighed. Triplicate silos were weighed and opened after 1, 3, 5, 7, 33, and 61 d. On d 61, $400{\pm}5g$ of material from each silo was placed in 1-L styrofoam containers, covered with cheesecloth and held at room temperature. Silage temperature was recorded hourly for 14 d via implanted thermocouple probes. Chemical composition of the forage at ensiling was consistent with previously reported values. At d 61, pH was lowest (p<0.01) in silage S. Ammonia-N was lower (p<0.05) in silage A than in silages S, B, E, or F. Compared to pre-ensiling values, water soluble carbohydrate concentrations were elevated in silages S, A, B, C and D, and decreased in E and F. Lactic acid concentrations were similar (p>0.10) across treatments. Acetic acid levels were highest (p<0.01) in silage E and lowest (p<0.01) in silage D. Recovery of DM was lower (p<0.01) in silage F than in silages S, A, B, C, or D. On d 61, yeasts were most numerous (p<0.01) in silage D, which was the only silage in which temperature rose more than $2^{\circ}C$ above ambient during aerobic exposure. Silage D also had the highest (p<0.01) pH and ADIN content after aerobic exposure. Lactic acid and WSC content of silage D decreased dramatically during the 14-d aerobic exposure period. Yeast counts (at d 14 of exposure) were lowest (p<0.01) in silages E and F. In general, the commercial inoculants did not appear to enhance the fermentation of barley silage to any appreciable extent in laboratory silos.
The effect of sodium hydroxide treatment on some physicochemical properties of zeolite mordenite mineral was studied with chemical analyses, powder X-ray diffraction, thermal analyses, infrared analysis, measurement of carbon dioxide adsorption and gas chromatography. Mordenite mineral from tuffaceous rocks in Yeongil and Wolsung area was used as a starting material and treated with 0.1-5N NaOH aqueous solution at about $95^{\circ}C$ in the water bath for three hours.At the concentration of sodium hydroxide below 0.5N, all chemical compositions in the tuff were virtually insoluble and the mordenite structure did not change. At the concentration above 1N, the chemical compositions such as silica, alumina, etc., were dissolved. The dissolution ratio of silica was lager than that of alumina, and the ratio of silica to alumina in the tuff decreased sharply in the concentration range of 2 to 3N. Intensity of X-ray diffraction peak of mordenite (202) plane and the adsorbed amount of carbon dioxide also decreased with the increasing concentration of sodium hydroxide above 1N. These decreases corresponded to the degree of mordenite structure collapsed.The separation of gas chromatography of nitrogen, oxygen and carbon monoxide was not affected by the sodium hydroxide treatment, but elution peaks of methane and krypton tended to be broadened and their retention time was shortened. The elution peaks of both methane and krypton tended to be overlapped with those of nitrogen and oxygen.
Park, Chul-Soo;Kang, Chon-Sik;Park, Jong-Chul;Jung, Youn-Joo;Cheong, Young-Keun;Woo, Sun-Hee
KOREAN JOURNAL OF CROP SCIENCE
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v.54
no.3
/
pp.249-259
/
2009
Puroindoline alleles, grain characteristics, milling performances and physical properties of flour of 22 Korean wheat cultivars were evaluated to determine the influence of puroindolines genotypes on grain and flour characteristics and to provide useful information for improving milling and end-use quality in Korean wheat breeding programs. Nine Korean wheat cultivars carried with Pina-D1a/Pinb-D1a, 11 cultivars had Pina-D1a/Pinb-D1b and 2 cultivars were Pina-D1b/Pinb-D1a. Korean wheats carrying with Pina-D1a/Pinb-D1a genotypes showed lower test weight and thousand kernel weight, area and roundness of grain and hardness index in grain characteristics, lower flour yield and higher proportion of break flour in milling performances and lower average particle size of flour, ash and damaged starch content, water retention capacity, yellowness-blueness and higher lightness of flour than wheats with Pina-D1a/Pinb-D1b or Pina-D1b/Pina-D1a genotypes. Pina-D1a/Pinb-D1b genotypes showed lower 1000-kernel weight, grain area, higher average of particle size of flour, higher ash and damaged starch content than Pina-D1b/Pina-D1a genotypes. There was no difference in hardness index of grain, milling performances, flour color between Pina-D1a/Pinb-D1b and Pina-D1b/Pina-D1a genotypes. These results could present the information to improve milling quality and physical properties of flour in Korean wheat breeding programs.
In-vessel retention through external reactor vessel cooling (IVR-ERVC) is a severe accident management (SAM) strategy that has been adopted and used in many nuclear reactors such as AP1000, APR1400, and light water reactor etc. Some reactor accidents have raised concerns about nuclear reactors among residents, leading to a decrease in residents' acceptability and many studies on SAM are being conducted. Experiments on IVR-ERVC are almost impossible due to its specificity, so fluid characteristics are analyzed through BALI experiments with similar condition. In this study, computational fluid dynamics (CFD) via Reynolds-averaged Navier-Stokes (RANS) and large eddy simulation (LES) for BALI experiments were performed. Steady-state CFD analysis was performed on three turbulence models, and SST k-ω model was in good agreement with the experimental measurement temperature within the maximum error range of 1.9%. LES CFD analysis was performed based on the RANS analysis results and it was confirmed that the temperature and wall heat flux for depth was consistent within an error range of 1.0% with BALI experiment. The LES CFD analysis results were compared with those of the Lagrangian-based solver. LES matched the temperature distribution better than SOPHIA, but SOPHIA calculated the position of boundary between stratified layer and convective layer more accurately. On the other hand, Lagrangian-based solver predicted several small eddy behaviors of the convective layer and LES predicted large vortex behavior. The vibration characteristics near the cooling part of the BALI experimental device were confirmed through Fast Fourier Transform (FFT) investigation. It was found that the power spectral density for pressure at least 10 times higher near the side cooling than near the top cooling.
The zooplankton community and environmental factor were investigated on a weekly basis from March to November 2015 in Lake Paldang, Korea. The seasonal succession of zooplankton community structure was influenced by hydraulic and hydrological factors such as inflow, outflow and rainfall. However, the hydraulic retention time in 2015 (16.3 day) was affected by the periods of water shortage that had continued since 2014 and increased substantially compared to 2013 (7.3 day). Therefore, the inflow and outflow discharge were decreased, and the water quality (COD, BOD, TOC, TP, Chl-a) of Lake Paldang (St.1) was the same characteristics as the river type Bukhan river (St.3), compared with the lake type Namhan river (St.2) and Gyeongan stream (St.4). Zooplankton community dominated by rotifers (Keratella cochlearis, Synchaeta oblonga) in spring (March to May). However, Copepod (Nauplius) and Cladoceran (Bosmina longirostris) dominated in St.4. In summer (June to August), there was a few strong rainfall event and the highest number of individuals dominated by Keratella cochlearis (Rotifera) and Difflugia corona (Protozoa) were shown during the study period. In autumn (October to November), the water temperature was decreased with decrease in the total number of individuals showing Nauplius (Copepoda) as the dominant species. As a result of the statistical analysis about zooplankton variation in environmental factors, the continuous periods of water shortage increased the hydraulic retention time and showed different characteristic for each site. St.1, St.3 and St.2, St.4 are shown in the same group (p<0.05), showing the each characteristics of river type and lake type. Therefore, the water quality of catchment area and distribution of zooplankton community would be attributed to hydraulic and hydrological factors.
Journal of the Korean Society of Food Science and Nutrition
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v.29
no.4
/
pp.586-591
/
2000
This study was conducted to investigate the effect on quality of low fat pork patties containing various hydrocoloids such as sodium alginate, carboxymethy1 cellulose (CMC) and zanthan gum. Moisture contents of raw, cooked and reheated patties of control patty (20% fat) were lower than those of the hydrocolloids-added patties, and fat content patties was higher than other patties. Colors of raw and reheated patties were not different among four kinds of patties, but L-value of cooked patty adding sodium alginate and b-value of control patty were low significantly in comparison with other patties. Cooking and final yield of hydrocolloids-added patties was high significantly in comparison with control patty. Fat retention of cooked control patty was lower than hydrocolloids-added patties, and fat retentions of retention four kinds of patties were 89.7~93.1%. Hardness of cooked and reheated patties adding xanthan gum was low significantly in comparison with other patties, and chewiness was high in theorder of control patty, CMC, sodium alginate and xanthan gum. In case of water holding capacity, cooked and reheated hydrocolloids-added patties, and juiciness was superior in the order of xanthan gum, sodium alginate, CMC and control patty. On the whole, textural and sensory characteristics of patties containing hydrocolloids were superior to control patty.
Two experiments were conducted to determine the effect of alkaline hydrogen peroxide (AHP) treatment (1% NaOH+1.5% $H_2O_2$; 1 AHPMS, 2% NaOH+1.5% $H_2O_2$; 2AHPMS) on rate and extent of degradation of mustard straw (MS) in sacco in sheep, and its in vivo digestion and ruminal fermentation characteristics when fed to sheep with concentrate (200 g per sheep daily). The treatment of straw with 1 and 2% AHP increased its sodium content by 148 and 296% to that of untreated straw (UMS). There was significant decrease in NDF and hemicellulose contents of AHP treated straw and increase in cellulose and lignin contents. Phenolic acids like ferrulic, $\rho$-coumaric and o-coumaric significantly (p<0.001) reduced by AHP treatment of mustard straw. In first experiment the in sacco degradation of DM, OM and NDF was significantly (p<0.01) greater for 2 AHPMS than for UMS at all incubation periods. The disappearance of nutrient from 1 AHPMS and 2 AHPMS treated straws continue to increase up to 96 h whereas in UMS the peak disappearance was found at 48 h. By using the equation {(y=a+b) ($1-e^{-ct}$)} the degradation rates (c) for DM, OM, and NDF were significantly higher for UMS than AHP treated straws. Level of alkali (1 and 2%) had significant effect on degradation characteristics (a, b, c and $P_{0.05}$) of DM and NDF fraction of MS. However, the effect was not pronounced on OM fraction of MS. In feeding experiment, the intake of nutrients for DM, OM, cell wall constituents and energy was higher on 2 AHPMS, whereas no effect on the digestibility of these nutrients was observed. The apparent nitrogen retention was higher (p<0.05) both in 1 and 2 AHPMS groups. Water intake by animals was significantly increased due to AHP treated mustard straw feeding. Rumen liquor pH was higher in 2 AHPMS fed animals. The $NH_3-N$ of rumen liquor was not affected by feeding of AHP treated MS based diets. Total VFA concentration was significantly (p<0.01) higher in UMS fed group. The fractional out flow rate of DM was higher (p<0.05) in animals fed on 2 AHPMS diets compared to UMS and lAHPMS fed groups. The population of large holotrichs was higher (p<0.05) on AHP treated MS fed diets compared to UMS. The study indicated that treatment of mustard straw with AHP changed its chemical composition towards a better feed. The nutritive value of 2% AHP treated mustard straw was better in terms of dry matter intake and apparent nitrogen retention. The higher in sacco DM, OM and NDF disappearance however, was not confirmed by in vivo data in this study.
Blue-green algae blooms occurred during early winter in the North-Han River, Korea. Among blue-green algae, Anabaena spiroides were observed for approximately 33 consecutive days, between 28 November and 30 December, 2011. A. spiroides emerged from Lake Uiam to Lake Paldang, depending on the flow of the river has spread downstream. Changes of physical water environment like rising water temperature and increasing hydraulic retention time influenced the A. spiroides bloom. The A. spiroides bloom showed a very rapid increase in cell density, and a slow decrease: the cell density increased to a maximum of $11,325cells\;mL^{-1}$ in Lake Paldang (st. 5), and was completely disappeared after the water temperature dropped below $4^{\circ}C$. A decrease in water temperature was the most influential factor among all environmental parameters, for the reduction of A. spiroides cell density. The A. spiroides bloom was accompanied with the occurrence of very high concentrations of the odor metabolite geosmin. Geosmin reached the peak value of $1,640ng\;L^{-1}$ in Lake Paldang (st. 4). The geosmin concentration was very strongly correlated with cell numbers of A. spiroides.
The purpose of this study was to determine the effects of using different amounts of potato puree (PP) (10 or 20%) and 10% bread crumbs (BC) as an extender and also control samples (C) with no added extender on chemical composition, energy values, cooking analyses, colour measurements, water holding capacity (WHC), penetration values, thiobarbituric acid value (TBA) and sensory analyses of meatballs. Meatball samples were cooked in a pre-heated $180^{\circ}C$ electric oven. Uncooked meatballs formulated with 20% PP had the highest moisture content. No significant differences were recorded for protein contents of uncooked samples. The highest cooking yield was found in samples extended with 10% BC. Increasing PP from 10% to 20% increased cooking yield of meatballs. 20% PP increased moisture and fat retention values and water holding capacity of meatballs. Meatballs with 10% BC had the lowest (the hardness in the texture) and meatballs with the 20% PP had the highest (the softness in the texture) penetration values. Formulating meatballs at a level of 20% resulted lower $L^*$ values. TBA values of control samples were higher than in PP added samples at the end of the storage period. Flavour scores for meatballs formulated with PP were higher than control and meatballs formulated with BC. Meatballs formulated with 10% PP had similar overall acceptability with meatballs added with 10% BC.
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