• Korean Journal of Acupuncture
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• 제39권4호
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• pp.152-171
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• 2022

Objectives : This study was designed to investigate the effects of Sunbanghwalmyung-eum gamibang on Monosodium iodoacetate-induced osteoarthritis rats. Methods : Forty Sprague-Dawley (SD) rats were divided into 5 groups of 8 rats each. Osteoarthritis (OA) was induced by injecting MIA (2 mg/50 µl) into the joint cavity of the left knee of SD rats belonging to the experimental group, and normal saline was injected into the joint cavity of the left knee instead of MIA in the normal group. To the normal group and the controlled group (OA group), 2 ml of distilled water was orally administered. To the positive control group (Indomethacin group), indomethacin 2 ml at a concentration of 2 mg/kg, to the low concentration group of SHG (Low group), 2 ml of SHG at a concentration of 2 mg/kg, and to the high concentration group of SHG (High group), 2 ml of SHG at a concentration of 4 mg/kg ml was orally administered. The drug was administered for a total of 4 weeks, and histological changes were analyzed by Hematoxylin-Eosin staining and Safranin-O staining. In addition, inflammatory cytokines such as TNF-α, IL-1β, and IL-6, and MMP-13, TIMP-1, and GAGs were immunohistochemically analyzed. Finally, hematological examination, blood biochemical examination, and liver and kidney biopsy were performed. Results : SHG groups (Low and High) inhibited the matrix destruction and damage of the knee joint cartilage in SD rat model, and significantly prevented the reduction in cartilage thickness. In SHG groups, the expressions of TNF-α, IL-1β, IL-6 and MMP-13 were significantly decreased, and the expressions of TIMP-1, GAGs were significantly increased compared with OA group. The safety indicators had no significant differences among five groups. Conclusions : These results show that SHG has cartilage protection capacity, anti-inflammatory effect.

• 한국구조물진단유지관리공학회 논문집
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• 제27권4호
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• pp.94-103
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• 2023

도심지에 건설되는 지하터널의 경우 터널 지상부 환경의 고려 뿐만 아니라 터널이 건설되는 지반 내부 환경의 변화를 정확히 파악하여 설계·시공해야한다. 하지만 현재 터널 설계에 적용되는 프로그램의 제한적 기능과 활용의 어려움으로 인해 지반응력, 지하수위 변화 등을 연계한 수치해석에 어려움이 있다. 이는 과다 설계로 이어져 공사비를 증가시키기 요인이 될 수 도 있고 부실 설계로 인해 시공 중 사고로 이어질 수 있는 문제가 발생할 수도 있다. 특히, 저토피 구간에 설치되는 도심지 터널의 경우 터널 상부 지층에 지하수위가 존재하며, 지하 공사 중 지하수위 저감으로 인해 상부지층의 유효응력이 증가되어 지반이 침하하는 현상이 발생할 수도 있으므로 지하수위 변화를 정확히 평가 후 설계를 해야한다. 따라서 본 연구에서는 터널 상부에 지하수위 층이 있는 도심지 지하터널을 대상으로 배수형태에 따른 응력-침투 연계해석을 통해 터널의 거동특성을 분석하였다.

• 대한본초학회지
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• 제38권5호
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• pp.9-19
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• 2023

Objectives : Osteoporosis is a systemic skeletal disorder characterized by reduced bone mineral density and increased risk of fractures. Bisphosphonates and selective estrogen receptors, which are bone resorption inhibitors that are currently widely used as osteoporosis treatments, show serious side effects when administered for a long time. Research on bone resorption inhibitors that complement the problems of existing treatments is needed. The purpose of this study was to investigate the effect of inhibiting osteoclast differentiation and activity on the tuberous root of Ampelopsis japonica (Thunb.) Makino (AM). Methods : After extracting AM using distilled water and ethanol, the inhibitory effects of the two solvents on osteoclast differentiation were compared using the RANKL-induced in vitro experimental model and the TRAP assay kit. The impact of AM on bone resorption was investigated through the pit formation assay, and its effect on F-actin formation was assessed through fluorescent staining. Additionally, protein and mRNA expression levels of osteoclast differentiation markers (NFATc1, c-Fos, TRAP and ATP6v0d2) and resorption markers (MMP-9, CTK, and CA2) were analyzed via western blot and RT-PCR. Results : AM treatment significantly decreased the number of TRAP-positive cells and pit formation area. Furthermore, AM suppressed both the protein and mRNA expression of NFATc1 and c-Fos, key transcription factors involved in osteoclast differentiation and it downregulated the expression of osteoclast-associated genes such as TRAP, CTK, MMP-9, CA2, and ATP6v0d2. Conclusions : These results suggest that AM can inhibit bone resorption and osteoclast differentiation, indicating its potential for use in the treatment and prevention of osteoporosis.

• Nutrition Research and Practice
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• 제17권2호
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• pp.371-385
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• 2023

BACKGROUND/OBJECTIVES: Soy isoflavone (SIF) and soy lecithin (SL) have beneficial effects on many chronic diseases, including neurodegenerative diseases. Regretfully, there is little evidence to show the combined effects of these soy extractives on the impairment of cognition and abnormal cerebral blood flow (CBF). This study examined the optimal combination dose of SIF + SL to provide evidence for improving CBF and protecting cerebrovascular endothelial cells. MATERIALS/METHODS: In vivo study, SIF50 + SL40, SIF50 + SL80 and SIF50 + SL160 groups were obtained. Morris water maze, laser speckle contrast imaging (LSCI), and hematoxylin-eosin staining were used to detect learning and memory impairment, CBF, and damage to the cerebrovascular tissue in rat. The 8-hydroxy-2'-deoxyguanosine (8-OHdG) and the oxidized glutathione (GSSG) were detected. The anti-oxidative damage index of superoxide dismutase (SOD) and glutathione (GSH) in the serum of an animal model was also tested. In vitro study, an immortalized mouse brain endothelial cell line (bEND.3 cells) was used to confirm the cerebrovascular endothelial cell protection of SIF + SL. In this study, 50 µM of Gen were used, while the 25, 50, or 100 µM of SL for different incubation times were selected first. The intracellular levels of 8-OHdG, SOD, GSH, and GSSG were also detected in the cells. RESULTS: In vivo study, SIF + SL could increase the target crossing times significantly and shorten the total swimming distance of rats. The CBF in the rats of the SIF50 + SL40 group and SIF50 + SL160 group was enhanced. Pathological changes, such as attenuation of the endothelium in cerebral vessels were much less in the SIF50 + SL40 group and SIF50 + SL160 group. The 8-OHdG was reduced in the SIF50 + SL40 group. The GSSG showed a significant decrease in all SIF + SL pretreatment groups, but the GSH showed an opposite result. SOD was upregulated by SIF + SL pretreatment. Different combinations of Genistein (Gen)+SL, the secondary proof of health benefits found in vivo study, showed they have effective anti-oxidation and less side reaction on protecting cerebrovascular endothelial cell. SIF50 + SL40 in rats experiment and Gen50 + SL25 in cell test were the optimum joint doses on alleviating cognitive impairment and regulating CBF through protecting cerebrovascular tissue by its antioxidant activity. CONCLUSIONS: SIF+SL could significantly prevent cognitive defect induced by β-Amyloid through regulating CBF. This kind of effect might be attributed to its antioxidant activity on protecting cerebral vessels.

• 한국지반환경공학회 논문집
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• 제24권4호
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• pp.5-11
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• 2023

수역 인근에서 산사태로 인해 발생되는 충격파는 인명과 주변 기반시설에 치명적인 피해로 이어질 수 있다. 이러한 충격파는 일반적으로 산사태 충격파라고 하며, 특정지역에 국한되지 않고 발생한다. 최근에는 이상기후의 영향으로 국지성 집중호우가 빈번하게 발생하면서 국내에서 발생하는 산사태의 발생빈도와 발생규모 모두 증가하고 있다. 이에 본 연구에서는 산사태 모형의 질량비 변화에 따른 실험을 수행하였으며, 생성되는 충격파의 특성 중 파의 진폭을 중점으로 관측 및 분석하였다. 본 연구에서는 5가지의 산사태 모형의 질량비, 3가지의 수로경사로 15가지의 케이스에 대하여 5회 반복실험하여 총 75회의 실험을 수행하였다. 산사태 모형의 질량비를 달리하며 실험을 해본 결과, 만약 초기 에너지가 동일한 산사태의 경우 순수한 입상, 순수한 블록 형태의 산사태에 의해서 생성되는 충격파의 크기보다 입상형태와 블록형태가 섞여 함께 거동하며 생성하는 충격파의 크기가 더 클 수 있다고 분석된다.

• 환경영향평가
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• 제32권2호
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• pp.83-93
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• 2023

본 연구는 남생이의 출현에 영향을 주는 환경변수를 파악하고 남생이와 환경변수 간의 관계를 파악하기 위해 전라남도 구례군의 주요 저수지에서 남생이 생태 특성을 고려한 17개의 환경변수를 선정하여 서식지 환경 조사를 실시하고, 서식지와 비서식지의 조사 결과를 통계 분석하였다. 남생이의 서식지 적합성 모델을 개발한 결과, 변수 예측값은 logit (p) = -3.68 + (0.17 × 낙엽층두께) + (1.55 × 육상아교목층 피도량) + (0.71 × 육상초본층 피도량) + (0.96 × 수상초본층 피도량)으로 산출되었다. 남생이는 낙엽층두께가 두꺼울수록, 육상교목층 피도량, 육상관목층 피도량, 수상초본층 피도량이 높을수록 출현확률이 높아지는 것으로 파악되었다. 남생이의 출현에 영향을 주는 이러한 환경요인에 대한 정보는 추후 남생이 분포조사 시 도움이 되며, 남생이의 실질적인 서식지 보전을 위해 필요할 것으로 판단된다.

• 한국지반환경공학회 논문집
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• 제24권7호
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• pp.5-14
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• 2023

최근 국내·외에서 지진이 자주 발생하고 있다. 이러한 지진은 다양한 형태의 자연적 피해와 물리적 피해의 원인이 된다. 특히, 지반이 액체와 같은 거동을 보이는 액상화는 구조물에 큰 피해를 발생시킨다. 이에 다양한 액상화 피해 저감 공법들이 연구 및 개발되고 있다. 이에 본 연구에서는 시트파일 공법을 이용하여 기존 구조물에 적용 가능한 지진 시 액상화 피해 저감 공법에 대해 연구하였다. 1-G 진동대 실험을 수행하였고 주문진 표준사로 지반을 조성하였다. 상사 법칙을 적용하여 2층 규모의 모형 구조물을 제작하였고, 입력파는 가속도 수준 0.6g와 주파수 10Hz의 정현파를 적용하였다. 다양한 근입비에 따른 구조물 피해 저감 효과를 분석하였다. 연구 결과, 시트파일 공법을 적용하여 지반을 보강하였을 때의 구조물 침하가 지반 보강을 하지 않았을 때보다 약 71% 정도 감소하였으며, 최소 침하량을 보인 근입비는 "1"이었다. 그리고 근입비가 증가할수록 시트파일로 인해 지반 내 간극수압의 소산이 지연되는 경향을 보였다. 이러한 결과를 바탕으로 근입비에 따른 구조물 침하와의 관계를 그래프와 관계식으로 제안하였다. 본 연구 결과는 향후 기존 구조물에 적용 가능한 시트파일 공법을 개발하는데 있어 기초자료로 사용될 수 있을 것으로 기대된다.

• Journal of Ginseng Research
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• 제47권3호
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• pp.458-468
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• 2023

Background: As a complication of Type II Diabetes Mellitus (T2DM), the etiology, pathogenesis, and treatment of cognitive dysfunction are still undefined. Recent studies demonstrated that Ginsenoside Rg1 (Rg1) has promising neuroprotective properties, but the effect and mechanism in diabetes-associated cognitive dysfunction (DACD) deserve further investigation. Methods: After establishing the T2DM model with a high-fat diet and STZ intraperitoneal injection, Rg1 was given for 8 weeks. The behavior alterations and neuronal lesions were judged using the open field test (OFT) and Morris water maze (MWM), as well as HE and Nissl staining. The protein or mRNA changes of NOX2, p-PLC, TRPC6, CN, NFAT1, APP, BACE1, NCSTN, and Ab1-42 were investigated by immunoblot, immunofluorescence or qPCR. Commercial kits were used to evaluate the levels of IP3, DAG, and calcium ion (Ca²⁺) in brain tissues. Results: Rg1 therapy improved memory impairment and neuronal injury, decreased ROS, IP3, and DAG levels to revert Ca²⁺ overload, downregulated the expressions of p-PLC, TRPC6, CN, and NFAT1 nuclear translocation, and alleviated Aβ deposition in T2DM mice. In addition, Rg1 therapy elevated the expression of PSD95 and SYN in T2DM mice, which in turn improved synaptic dysfunction. Conclusions: Rg1 therapy may improve neuronal injury and DACD via mediating PLC-CN-NFAT1 signal pathway to reduce Aβ generation in T2DM mice.

• Earthquakes and Structures
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• 제24권6호
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• pp.455-475
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• 2023

The present study investigates the non-linear soil-pile interaction using three-dimensional (3D) non-linear finite element models. The numerical models were validated by using the results of extensive pile load and shaking table tests. The pile performance in liquefiable and non-liquefiable soil has been studied by analyzing the liquefaction ratio, pile lateral displacement (LD), pile bending moment (BM), and frictional resistance (FR) results. The pile models have been developed for the different ground conditions. The study reveals that the results obtained during the pile load test and shaking cycles have good agreement with the predicted pile and soil response. The soil density, peak ground acceleration (PGA), slenderness ratio (L/D), and soil condition (i.e., dry and saturated) are considered during modeling. Four ground motions are used for the non-linear time history analyses. Consequently, design charts are proposed depended on the analysis results to be used for design practice. Eleven models have been used to validate the capability of these charts to capture the soil-pile response under different seismic intensities. The results of the present study demonstrate that L/D ratio slightly affects the lateral displacement when compared with other parameters. Also, it has been observed that the increasing in PGA and decreasing L/D decreases the excess pore water pressure ratio; i.e., increasing PGA from 0.1 g to 0.82 g of loose sand model, decrease the liquefaction ratio by about 50%, and increasing L/D from 15 to 75 of the similar models (under Kobe earthquake), increase this ratio by about 30%. This study reveals that the lateral displacement increases nonlinearly under both dry and saturated conditions as the PGA increases. Similarly, it is observed that the BM increases under both dry and saturated states as the L/D ratio increases. Regarding the acceleration histories, the pile BM was reduced by reducing the acceleration intensity. Hence, the pile BM decreased to about 31% when the applied ground motion switched from Kobe (PGA=0.82 g) to Ali Algharbi (PGA=0.10 g). This study reveals that the soil conditions affect the relationship pattern between the FR and the PGA. Also, this research could be helpful in understanding the threat of earthquakes in different ground characteristics.

• 한방재활의학과학회지
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• 제33권3호
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• pp.1-15
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• 2023

Objectives We aimed to find out the improvement effect of Baekhogainsam-tang (Baihu Jia Renshen-tang, BIT) on metabolic syndrome and alteration of microbiota and gene expression. Methods We used male C57BI/6 mice and randomly assigned them into three groups. Normal control group was fed 10% kcal% fat diet, high-fat diet (HFD) group was fed 45% kcal% fat diet and 10% fructose water. BIT group was fed same diet as HFD group and treated by BIT for once daily, 6 days per week, total 8 weeks. We measured their body weight and food intake every week and performed oral glucose tolerance test 1 week before the end of the study. Then we collected the blood sample to measure triglyceride, total cholesterol, high-density lipoprotein cholesterol, insulin, and hemoglobin A1c. We harvested tissue of liver, muscle, fat, and large intestine for quantitative polymerase chain reaction (qPCR) and histopathological examination. Fresh fecal samples were collected from each animal to verify alterations of gut microbiota and we used RNA from liver tissue for microarray analysis. Results The body weight and fat weight of BIT group were reduced compared to HFD group. The qPCR markers usually up-regulated in metabolic syndrome were decreased in BIT group. Bacteroides were higher in BIT group than other groups. There were also differences in gene expressions between two groups such as Cyp3a11 and Scd1. Conclusions We could find out BIT can ameliorate metabolic syndrome and suggest its effect is related to gut microbiota composition and gene expression pattern.