• Mass Spectrometry Letters
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• 제11권1호
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• pp.10-14
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• 2020

Riboflavin is a water-soluble vitamin, which serves as a precursor to flavin mononucleotide and flavin adenine dinucleotide. This study aimed to develop a simple and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis for the quantification of riboflavin in the Beagle dog plasma. This method utilized simple protein precipitation with acetonitrile and ¹³C₄, ¹⁵N₂-riboflavin was used as an internal standard (IS). For chromatographic separation, a hydrophilic interaction liquid chromatography (HILIC) column was used with gradient elution. The mobile phase consisted of 0.1% (v/v) aqueous formic acid with 10 mM ammonium formate and acetonitrile with 0.1% (v/v) formic acid. Since riboflavin is an endogenous compound, 4% bovine serum albumin in phosphate buffered saline was used as a surrogate matrix to prepare the calibration curve. The quantification limit for riboflavin in the Beagle dog plasma was 5 ng/mL. The method was fully validated for its specificity, sensitivity, accuracy and precision, recovery, and stability according to the US FDA guidance. The developed LC-MS/MS method may be useful for the in vivo pharmacokinetic studies of riboflavin.

• Bulletin of the Korean Chemical Society
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• 제24권9호
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• pp.1319-1323
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• 2003

This paper presents a successful demonstration of the on-line coupling of MEKC (micellar electrokinetic chromatography) to ESIMS (electrospray ionization mass spectrometry) for the quantitative analysis of seven s-triazine herbicides. The on-line MEKC-ESIMS was used to determine the structure of CE-separated peaks of seven triazine herbicides. The mixture of triazine herbicides was separated in a 20 mM sodium borate buffer (pH 8.5) containing 15 mM sodium dodecylsulfate (SDS) by using a bare fused-silica capillary. Electrospray ionization mass spectrometer was operated in the positive-ion mode when the mass spectra of seven triazine herbicides were observed from each peak, and the solution of water-methanol-formic acid (50/49/1 v/v/v) was used as a sheath liquid. The effects of SDS concentration, the run buffer pH, and the electric field on the separation of seven s-triazine herbicides were investigated. The MEKC-ESIMS detection showed 5 to 10 times higher sensitivity compared to the MEKC-UV detection. In addition, it did not need any pretreatment step.

• 한국치위생학회지
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• 제13권1호
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• pp.158-165
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• 2013

Objectives : The purpose of this study was to obtain the community residents' support and approval on the water fluoridation program in Mokpo, Korea. Methods : Questionnaires of this were 1085 adults, over the age of 20, who resided in the Mokpo city for over the 1year. In order to analyze the factors that were related to the approval on administering water fluoridation program, the data analyzed using a chi-squared test and a multiple logistic regression analysis. Results : Respondents approving the implementation of water fluoridation were 50.5%. The general factors of approval were age, education, Monthly household income. The knowledge factors of approval were experience of hearing of this program, the knowledge of the purpose of this program, the knowledge of the some regions in South Korea had been implement water fluoridation. The results of multiple logistic regression analysis were the awareness of oral health and the knowledge level of water fluoridation were related with this program approval. Conclusions : In order to enhance the rate of approval on administrating the water fluoridation, it is necessary to publicize the purpose, effect, and stability of this program, through a diverse mass media and reinforce the health education program to oral health management.

• Journal of Ginseng Research
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• 제32권4호
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• pp.390-396
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• 2008

For evaluating the quality of ginseng, simple and fast analysis methods are needed to determine the ginsenoside content of the ginseng products. The aim of this study was therefore to optimize conditions for fast analysis of the ginsenosides, the active ingredients in extracts of Korean red ginseng. When tandem HPLC mass spectrometry (HPLC-MS/MS) was used, four forms of ginsenoside, Rb1, Rb2, Rc, and Re, were readily separated in seven minutes using a gradient mobile phase (acetonitrile and water containing acetic acid). This is the shortest separation time reported among the studies of major ginsenoside analysis. When gradient HPLC with UV detection was used, the detection limit was high, but separation of these four ginsenosides required 25 minutes using acetonitrile and water containing formic acid as a mobile phase. HPLC-MS/MS was able to separate ginsenoside Rg1 easily regardless of the mobile phase condition, but the HPLC-UV could not separate Rg1 because acetonitrile concentration in the mobile phase had to be maintained below 20%. Ginsenoside peaks were clearer and had more sensitive detection limits when Korean red ginseng extract was analyzed by the HPLC-MS/MS, but the UV detection was useful for chromatographic fingerprinting of all four major ginsenosides of the extract: Rb1, Rb2, Rc, and Re. Extracts were found to contain 2.17 mg, 1.51 mg, 1.29 mg, and 0.46 mg of ginsenoside Rb1, Rb2, Rc, Re, respectively, per gram weight. The ratios of each ginsenoside in the extracts were 1.0 : 0.7 : 0.6 : 0.2, respectively. Taken together, the results indicate that HPLC-MS/MS spectrometry could be the most useful method for rapid analysis of even small amounts of major ginsenosides, while HPLC with UV detection could also be used for rapid analysis of major ginsenosides and for quality control of ginseng products.

• 한국도로학회논문집
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• 제17권5호
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• pp.1-10
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• 2015

PURPOSES : A finite difference model considering snow melting process on porous asphalt pavement was derived on the basis of heat transfer and mass transfer theories. The derived model can be applied to predict the region where black-ice develops, as well as to predict temperature profile of pavement systems where a de-icing system is installed. In addition, the model can be used to determined the minimum energy required to melt the ice formed on the pavement. METHODS : The snow on the porous asphalt pavement, whose porosity must be considered in thermal analysis, is divided into several layers such as dry snow layer, saturated snow layer, water+pavement surface, pavement surface, and sublayer. The mass balance and heat balance equations are derived to describe conductive, convective, radiative, and latent transfer of heat and mass in each layer. The finite differential method is used to implement the derived equations, boundary conditions, and the testing method to determine the thermal properties are suggested for each layer. RESULTS: The finite differential equations that describe the icing and deicing on pavements are derived, and we have presented them in our work. The framework to develop a temperature-forecasting model is successfully created. CONCLUSIONS : We conclude by successfully creating framework for the finite difference model based on the heat and mass transfer theories. To complete implementation, laboratory tests required to be performed.

• Nuclear Engineering and Technology
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• 제56권2호
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• pp.536-545
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• 2024

Radioactive iodine is a representative fission product to be quantified for the safety assessment of nuclear facilities. In integral severe accident analysis codes, the iodine behavior is usually described by a multi-physical model of iodine chemistry in aqueous phase under radiation field and mass transfer through gas-liquid interface. The focus of studies on iodine source term evaluations using the combination approach is usually put on the chemical aspect, but each contribution to the iodine amount released to the environment has not been decomposed so far. In this study, we attempted the decomposition by revising the two-film theory of molecular-iodine mass transfer. The model involves an effective overall mass transfer coefficient to consider the iodine chemistry. The decomposition was performed by regarding the coefficient as a product of two functions of pH and the overall mass transfer coefficient for molecular iodine. The procedure was applied to the EPICUR experiment and suppression chamber in BWR.

• Geomechanics and Engineering
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• 제8권3호
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• pp.323-359
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• 2015

This paper presents an elasto-plastic model for determination of the ground response curve of a circular underwater tunnel excavated in elastic-strain softening rock mass compatible with a nonlinear Hoek-Brown yield criterion. The finite difference method (FDM) was used to propose a new solution to calculate pore water pressure, stress, and strain distributions on periphery of circular tunnels in axisymmetric and plain strain conditions. In the proposed solution, a modified non-radial flow pattern, for the hydraulic analysis, is utilized. To evaluate the effect of gravitational loads and variations of pore water pressure, the equations concerning different directions around the tunnel (crown, wall, and floor) are derived. Regarding the strain-softening behavior of the rock mass, the stepwise method is executed for the plastic zone in which parameters of strength, dilatancy, stresses, strains, and deformation are different from their elasto-plastic boundary values as compared to the tunnel boundary values. Besides, the analytical equations are developed for the elastic zone. The accuracy and application of the proposed method is demonstrated by a number of examples. The results present the effects of seepage body forces, gravitational loads and dilatancy angle on ground response curve appropriately.

• 한국안전학회지
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• 제31권2호
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• pp.76-82
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• 2016

A 2-D hydrodynamic model for predicting the movement of debris flow was developed. The developed model was validated against a dam break flow problem conducted in EU CADAM project, and the performance of the model was shown to be satisfactory. In order to suggest structural alternative for hazardous zone vulnerable to debris flow disaster, two types of initial mass distribution and two shapes of defensive structure were considered. It was found that 1) the collapse of debris mass initiated with square pyramid shape induced more damage compared with that of cubic shape; and 2) a defensive structure with semi-circular shape was vulnerable to debris flow disaster in terms of debris control or primary defense compared with that of rectangular-shaped structure.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2013년도 춘계학술대회 논문집
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• pp.83-88
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• 2013

The purpose of this study is to develop the algorithm for dynamic interaction analysis of submerged floating tunnel and vehicles. The dynamic behavior characteristic of submerged floating tunnel is certainly different with general structures, because the submerged floating tunnel is floating in the middle of water, and subjected to constant buoyance. Therefore the analyses in various aspects should be carried out to secure structural stability and practicality of structures. To conduct the dynamic interaction analysis, the structure is modeled by commercial FEM program ABAQUS to investigate modal characteristic. Also the added mass concept is applied to represent the inertial force by a fluid, and then dynamic interaction analyses are conducted with superposition method when the KTX is moving along the submerged floating tunnel. And the time histories are presented for vertical and lateral displacement at the center of the tunnel.

• 한국초전도ㆍ저온공학회논문지
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• 제1권1호
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• pp.48-55
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• 1999

thermodynamic cycle analysis has been performed for the power generation systems to utilize the cold energy of liquefied natural gas (LNG). The power cycle used the air or water at room temperature as a heat source and the LNG at cryogenic temperature as a heat sink. Among manypossible configurations of the cycle. the open Rankine cycle. and the closed Brayton cycle, and the closed Rankine cycle are selected for the basic analysis because of their practical importance. The power output per unit mass of LNG has been analytically calculated for various design parameters such as the pressure ratio. the mass flow rate. the adiabatic efficiency. the heat exchanger effectiveness. or the working fluid. The optimal conditions for the parameters are presented to maximize the power output and the design considerations are discussed. It is concluded that the open Rankine cycle is the most recormmendable both in thermodynamic efficency and in practice.