• Journal of Applied Biological Chemistry
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• v.55 no.4
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• pp.207-215
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• 2012

Phenolic compounds of $17.9{\pm}1.0mg/g$ were extracted from Ulmus pumila with 70% ethanol. The elastase inhibitory activity related with forming wrinkle was shown an excellent wrinkle improvement effect in 70% ethanol extracts as 55.5-69.5% at phenolic concentration of $50-200{\mu}g/mL$. The tyrosinase inhibitory activity related with skin-whitening was 24% in 70% ethanol extracts at phenolic concentration of $200{\mu}g/mL$. The astringent activity of 70% ethanol extracts was shown activity of 71% at phenolic concentration of $200{\mu}g/mL$ therefore it is judged that there is a high effect on pores reduction of the skin. The hyaluronidase inhibitory activity of U. pumila extracts was confirmed anti-inflammation effect of 80% at phenolic concentration of $50{\mu}g/mL$. Antimicrobial activity of U. pumila water extracts was shown each 8.7, 10.0, 11.1 and 11.8 mm clear zones on Propionebacterium acnes at phenolic concentration of $50-200{\mu}g/mL$. The stability of the multi-functional cosmetic (lotion) added U. pumila extracts was very stable for 28 days without changing of pH and viscosity also it's stable on temperature and sun lights. As the concentration of extracts was increased, the color of lotion was getting dark, but the sensory evaluation was high at score of 8.5.

• Journal of Applied Biological Chemistry
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• v.52 no.2
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• pp.65-69
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• 2009

In this study, we tried to find the subject to inhibit H. pylori from Cheongmoknosang mulberry leaves extracts and to purify and identify them. Total phenolic compounds of hot water and 80% ethanol extracts are 17.6 and 16.1 mg/g. The activity of H. pylori inhibition at 80% ethanol extracts was determined as 15mm clear zone. The purification of inhibitory compounds were carried on $C_{18}$ column and MCI-gel CHP-20 column chromatography which were used a gradient procedure as increasing ethanol in $H_2O$. The chemical structure of purified inhibitory compounds on H. pylori were identified chlorogenic acid, caffeic acid, and rosmarinic acid by FAB-MS, $^1H-NMR$, $^{13}C-NMR$ and IR spectrum.

• Journal of Animal Science and Technology
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• v.44 no.5
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• pp.633-642
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• 2002

Enteric colibacillosis has economically become an important disease of young animals as a result of increasing intensification of farrowing management. The objective of this experiment is to isolate fimbrial antigen from enterotoxigenic E. coli F41, to develop specific polyclonal IgY which can effectively neutralize or reduce the proliferation of pathogens in feed or living animal system, and to apply IgY technologies to animal industry. The results obtained were as follows: The molecular weight of the purified F41 antigen was 29,500 dalton on sodium dodecyl sulfate-polyacrylamide gels. Fimbrial antigen was confirmed by the western blot method. It was observed that after immunization the level of serum antibody titer of laying hen was shown in two weeks and gradually increased. The antibody titer in egg yolk appeared two weeks after it was shown in serum antibody. The titers of egg yolk antibody were gradually increased to the maximum level of 320,000 (antigen 50${\mu}g$/$m\ell$), 450,000 (antigen 200${\mu}g$/$m\ell$), and 320,000 (antigen 600${\mu}g$/$m\ell$). According to the results of specificity test by ELISA, the anti-F41 antibodies from chicken serum and egg yolk reacted only with ETEC F41 antigen. There was no cross reaction with other ETEC strains (K88, K99, and 987P). In vitro condition, as a result of antigen binding ability of yolk antibodies, bacterial concentration was rapidly decreased to $10^5$ CFU/$m\ell$ from $10^9$ CFU/$m\ell$ when 2${\sim}$4 mg/$m\ell$ of freeze dried WSF (water soluble fraction) was added.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1996.05a
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• pp.91-115
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• 1996

In the recent years, protected horticultural facilities have been modernized and glasshouses are also propagating in Korea, even most vegetables production are conducted in the traditional plastic houses covered with, for example, PVC film for just temperature keeping. It would limit the productivity and competitivity of the vegetable production industry without automatization and high quality year round production. A plant factory, aimed to produce vegetables in the limited areas, was initiated in Christensen farm, Denmark in 1957, and widely propagated in some developed countries. As it has the automatized system which enables to keep optimized environment conditions, it will be the best facility for high quality products as well as year round planned production. However, we have not even started the plant factory production. Since the plant factory is requiring lots of resources, besides plant cultivation technologies, such as environment control, automatic engineering and robotics, our approach to the development of plant factories should be minded on Practical Plant Factories considering our current farming practices and least capital needs rather than blindly employing the advanced technologies from developed countries. Thus, Korean plant factory development can be initiated with year round leaf vegetables production in NFT or DFT cultivation system instead of the moval bed system, in which aerial environment factors such as light, temperature, humidity and CO$_2$ concentration and root environment ones such as solution concentration, temperature, pH and water soluble oxygen shall be automatically controlled. And the seeding, seedling and transplanting operations shall be accomplished in the house entrance, and the harvesting and grading opreations shall be conducted in the house exit. For practical plant factories, environment control technologies including artificial light source, illumination and air conditioning, automatic management for nutrient solution and automatic production line of moval bed system, transplanting and harvest should be developed along with researches on the cost reduction of factory building construction.

• Journal of the Korean Applied Science and Technology
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• v.35 no.3
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• pp.702-708
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• 2018

Hydrogels are natural polymer networks that can contain huge quantities of water and many cosmetical ingredients. Their hydrophilic functional groups creates a matrix, which allows high efficacy in delivering active ingredients into the skin. In industry, hydrating properties and strength of the hydrogels are of great interest in manufacturing hydrogel mask packs. We have used the cellulose in various forms such as powder, cotton fiber and cellulase treated cotton fiber to investigate the property changes of cellulose/hydrogel sheets. When 0.1% and 0.3% of cellulose powder were added to hydrogels, tensile strength of hydrogel sheets were decreased by 10% and 14% respectively. Vise versa, when 0.5 ~ 2 cm of cotton fibers were added, tensile strength of hydrogel sheets were significantly increased by about 20%. The hydrogels which contain cotton fibers also gave an excellent moisturizing effect. Especially cellolose/hydrogels containing cellulase-treated cotton fibers showed the best effect on retaining moisture content increasing upto 380% in comparison with the one containing untreated cotton as well as excellent dispersibility.

• Journal of the Korean Applied Science and Technology
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• v.37 no.4
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• pp.1041-1051
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• 2020

The technology of microbubbles and nanobubbles originated in Japan and Europe is applicable to various applications and its effects are diverse, attracting attention not only from many researchers but also from industry experts. In particular, nanobubbles have the advantage that they can be applied to products in the form of liquids, such as cosmetics, from the study that they can exist for more than several months in water. In this study, it was carried out the production of nanobubbles using bubble encapsulation technique and the experiment of skin permeability enhancement of active substances in cosmetics using nanobubble techniquethree. Nanobubbles were confirmed to affect the skin permeability increase of active substances, and up to 250% increase in skin permeability compared to non-bubbles-free materials(Caffeic acid, at 8 hour). It is expected that research results and industrial ripple effects can be expected not only in the cosmetic field, but also in fields applicable to the improvement of permeability by nanobubble techniques, such as areas related to drug delivery system.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.6
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• pp.843-851
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• 2003

An experiment was conducted to investigate the effects of pre-wilting Bana grass (Pennisetum purpureum x P. americanum) herbage under sunny conditions for 0, 6, 18, 24, 32 and 48 h and ensiling it with maize meal. Four levels of maize meal(viz., 0, 5, 10 and 15% on fresh weight (Fw) basis) were tested. The experiment had a split-plot design. Wilting increased the concentration of water soluble carbohydrates (WSC) significantly (p<0.001) on a Fw basis, although there were no significant changes on DM basis. Unwilted grass contained $36.1g{\cdot}WSC{\cdot}kg^{-1}{\cdot}Fw$ ($127.6g{\cdot}kg^{-1}{\cdot}DM$) and this increased to $64.1g{\cdot}WSC{\cdot}kg^{-1}{\cdot}Fw$ ($116.7g{\cdot}kg^{-1}{\cdot}DM$) after 48 h of pre-wilting. Wilting also increased the DM content of herbage significantly (p>0.001) from 250 to $620g{\cdot}kg^{-1}$, between 0 and 48 h respectively. The concentration of fermentation end-products decreased (except butyric acid) and pH increased when the period of wilting increased, indicating that fermentation was restricted. In particular, lactic acid content declined from 50.8 to $26.2g{\cdot}kg^{-1}{\cdot}DM$ (p<0.01) and the residual WSC contents of silage increased from 2.7 with fresh herbage to $18.1g{\cdot}kg^{-1}{\cdot}DM$ with 48 h of wilting (p<0.001). Rapid wilting for 24 h, to a DM of $450g{\cdot}kg^{-1}$ was optimum since important increases in pH, residual WSC and DMD occurred at this level of wilting. Acetic acid, butyric acid and ammoniacal-N contents were lowest with 24 h of wilting. There were no significant interactions between length of wilting and the incorporation of maize meal. Wilting had a greater influence on fermentation than the incorporation of maize meal. Addition of maize meal facilitated fermentation by increasing forage DM content and reducing effluent production. In addition, the maize meal increased DMD. It was concluded that maize meal should generally be incorporated at a level of 5% on fresh weight basis.

• Nutrition Research and Practice
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• v.12 no.4
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• pp.336-341
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• 2018

BACKGROUND/OBJECTIVES: Dietary Reference Intakes (DRI) for energy are derived from total energy expenditure (TEE) measured using the doubly labelled water (DLW) method. The objective of this study was to assess the validity of DRI for predicting the energy requirements of elementary school-age children. SUBJECTS/METHODS: The present study involved 25 elementary school-age children aged between 9 and 11 years. TEE was assessed by the DLW method, and the results were compared with the TEE predicted by the DRI equations in order to evaluate accuracy. RESULTS: The subjects' TEE measured by the DLW method was $1,925.2{\pm}380.9kcal/day$ in boys and $1,930.0{\pm}279.4kcal/day$ in girls, whereas resting energy expenditure was $1,220.2{\pm}176.9kcal/day$ in boys and $1,245.9{\pm}171.3kcal/day$ for girls. The physical activity level was $1.58{\pm}0.20$ in boys and $1.55{\pm}0.13$ in girls. The mean bias between the predicted and measured TEE was 12.6% in boys and -1.6% in girls, and the percentage of accurate predictions was 28.6% and 63.6%, respectively. In boys, the equation resulted in underprediction of TEE among the subjects having low TEE values, whereas there was overprediction among subjects having high TEE values as shown by the Bland-Altman plot. On the contrary, this proportional bias was not observed in girls. CONCLUSIONS: The findings of this study suggest that the DRI equation for energy could result in the overestimation of energy requirements in elementary school-age boys. In the case of girls, the equations could be accurate at the group level. However, the DRI appears to be invalid for individual girls, as more than one third of girls had their TEE inaccurately predicted. We recommend more studies for confirmation of these results.

• Journal of the Korean Institute of Landscape Architecture
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• v.18 no.4
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• pp.45-71
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• 1991

The landscape systems in Korean island settlements can be recognized as results of ingabitants' ecological adptation to the isolated environment with the limited natural resources. Both the fishery dominant industry in island society and ecological nature of its environments seem to have influenced on inhabitants' environmental cognition as well as the physical landscape of island villages such as its location, spatial pattern in each village, housing form and so on. This study was done mainly by both refering to the related documents and direct observations in case study areas, and results of the study can be summarized as follows. 1. In general, the landscape of an individual island seems to take more innate characteristics of island's own, corresponding to the degree of isolation from mainland. That is, while the landscape of island in neighboring waters takes both inland-like and island-innate landscape character at the same time, the one in the open sea far from land takes more innate landscape character of all island's own in the aspects of village location, land use and housing density etc. 2. The convex landform of most islands brings about more centrifugal village allocation than centripetal allocation in most inland villages. And thus most villages in each island face extremely diverse directions different from the south facing preference in most inland rural villages. 3. Most island villages tend to be located along the ecologically transitional strip between land and sea, so called 'line of life', rather than between hilly slope and flat land as being in most inland village locations. So they are located with marine ecology bounded fishing ground ahead and land ecology bounded agricultural site at the back of them. 4. The settlement pattern of the island fishing villages shows more compact spatial structure than that of inland agricultural villages, due to the absolute limits of usable land resources and the adaptation to the marine environment with severe sea winds and waves or for the easy accessability to the fishing grounds. And also the managerial patterns of public owned sea weed catching ground, which take each family as the unit of usership rather than an individual, seem to make the villagescape more compact and the size of Individual residence smaller than that of inland agricultural village. 5. The folk shrine('Dand') systems, in persrective of villagescape, represent innate environmental cognition of island inhabitants above all other cultural landscape elements in the island. Usually the kinds and the meanings of island's communal shrine and its allocative patternsin island villagescape are composed of set with binary opposition, for example 'Upper shrine(representing 'earth', 'mountain' or 'fire')' and 'Lower Shrine(representing 'sea', 'dragon' or 'water') are those. They are usually located at contrary positions in villagescape each other. That is, they are located at 'the virtical center or visual terminus(Upper shrine at hillside behind the village)' and 'the border or entrance(Lower Shrine at seashore in front of the village)'. Each of these shirines' divinity coincides with each subsystem of island's natural eco-system(earth sphere vs marine sphere) and they also contribute to ecological conservation, bonded with the 'Sacred Forest(usually with another function of windbreak)' or 'Sacred Natural Fountain' nearby them, which are representatives of island's natural resources.

• Journal of Korean Society for Atmospheric Environment
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• v.29 no.1
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• pp.97-104
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• 2013

Methyl alcohol is one of the basic intermediates in the chemical industry and is also being used as a fuel additive and as a clean burning fuel. In this study, conversion of carbon dioxide to methyl alcohol was investigated using catalytic chemical methods. Ceramic monoliths (M) with $400cell/in^2$ were used as catalyst supports. Monolith-supported CuO-ZnO catalysts were prepared by wash-coat method. The prepared catalysts were characterized by using ICP analysis, TEM images and XRD patterns. The catalytic activity for carbon dioxide hydrogenation to methyl alcohol was investigated using a flow-type reactor under various reaction temperature, pressure and contact time. In the preparation of monolith-supported CuO-ZnO catalysts by wash-coat method, proper concentration of precursors solution was 25.7% (w/v). The mixed crystal of CuO and ZnO was well supported on monolith. And it was known that more CuO component may be supported than ZnO component. Conversion of carbon dioxide was increased with increasing reaction temperature, but methyl alcohol selectivity was decreased. Optimum reaction temperature was about $250^{\circ}C$ under 20 atm because of the reverse water gas shift reaction. Maximum yield of methyl alcohol over CuO-ZnO/M catalyst was 5.1 mol% at $250^{\circ}C$ and 20 atm.