• Title/Summary/Keyword: Water Yield

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Methods for the Extraction of DNA from Water Samples for Polymerase Chain Reaction

  • Jung, Jae-Sung;Lee, Young-Jong
    • Journal of Microbiology
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    • v.35 no.4
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    • pp.354-359
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    • 1997
  • Methods for the extraction of DNA from water sample were approximated. Four different procedures of DNA extraction were carried out with pellets obtained from centrifugation of 4 liter water samples. The recovery efficiency and purity of DNA extracted by each method from different sources were compared. DNA yield varied with extraction methods, Method I, which involves enzymatic and freeze-thaw lysis steps and phenol and phenol-chloroform purification of extracted nucleic acid, showed a significantly higher yield and purity than the other methods. The use of glass beads in the DNA extraction methods improved the purity of DNA suitable for PCR. Bovine serum albumin in the PCR reaction mixture was useful in reducing inhibitory effects of contaminants. The efficiency of an extraction method was determined by the detection of the aer of Aeromonas hydrophila with PCR. The lower limit of detection of A. hydrophila from seeded tap water was 2 CFU/ml in PCR when method I was used for DNA preparation.

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An Evaluation study on Total Nitrogen(T-P) Item of Agricultural Water Standards (농업용수 수질기준 T-P 항목에 대한 검증실험(I))

  • Choi, Sun-Hwa;Kim, Ho-Il;Yoon, Kyung-Seup;Lee, Bong-Hun
    • Proceedings of the Korean Society of Agricultural Engineers Conference
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    • 2003.10a
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    • pp.603-606
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    • 2003
  • This study was carried out to investigate the effects of T-P concentrations in irrigation water on the growth, yield, and grain quality of rice. It acquire fundamental knowledges to set up irrigation water quality standards. The pot experiment was conducted with 5 treatments using irrigation waters. It consisted of various total phosphorus concentrations(control, 1, 2, 5, 10mg/L) and replicated four times with randomized block design. The results of this study showed that as T-P concentrations in irrigation water increases, plant height tended to increase. Dry weight of rice plant at T-P 10mg/L was significantly higher than the control. However, T-P in irrigation water did not affect plant height, tiller number, plant dry weight, yield, and quality of rice.

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Rheological Properties According to the Hardness of Dampening solution for Lithographic Ink (축임물의 경도에 따른 평판인쇄잉크의 유동성 변화)

  • Cho, Jin-Woo;Kim, Sung-Bin;Park, Jeung-Min
    • Journal of the Korean Graphic Arts Communication Society
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    • v.20 no.2
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    • pp.7-18
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    • 2002
  • The emulsification of lithographic printing inks are very important to determine printing quality. In this study, we measured the variation of water pickup according to the hardness of water in dampening solution. In order to observe the rheorogical properties of the emulsified inks, we measured the viscosity, the elastic return, the thixotropy area and yield stress value of the emulsified inks. In this paper, we found that the water pickup of the ink increase the hardness of water with the amount of dampening solution. Also, The viscosity, the yield stress value and the thixotropy area were increase by the water pickup of the ink.

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Physiological Activity of Acai Berry (Euterpe oleracea Mart.) Extracted with Different Solvents (추출용매에 따른 아사이 베리의 생리활성)

  • Chung, Hai-Jung
    • Journal of the Korean Society of Food Culture
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    • v.27 no.1
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    • pp.75-81
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    • 2012
  • This study was conducted to investigate the physiological activity of acai berry (Euterpe oleracea Mart.) extracts from three different solvents (water, methanol, and ethanol). We measured total polyphenol and total flavonoid content, DPPH radical scavenging activity, nitrite scavenging activity, metal chelating effect, and reducing power. The extraction yield from water, methanol, and ethanol was 17.10, 9.50, and 37.51%, respectively. The highest total polyphenol content (10.54 mg/100 g) and total flavonoid contents (1.88 mg/100 g) was observed in water extract. DPPH radical scavenging activity was the highest in both water extract (72.03%) and methanol extract (74.79%) at levels of 5 mg/mL, which was similar to that of BHT (78.90%). Water extract yielded the highest metal chelating effect (92.54%) and reducing power (1.09) at levels of 5 mg/mL. Taken together, these findings suggest that extracts of acai berry can be used as functional food materials with antioxidative and nitrite scavenging activities.

Assessment of Improving SWAT Weather Input Data using Basic Spatial Interpolation Method

  • Felix, Micah Lourdes;Choi, Mikyoung;Zhang, Ning;Jung, Kwansue
    • Proceedings of the Korea Water Resources Association Conference
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    • 2022.05a
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    • pp.368-368
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    • 2022
  • The Soil and Water Assessment Tool (SWAT) has been widely used to simulate the long-term hydrological conditions of a catchment. Two output variables, outflow and sediment yield have been widely investigated in the field of water resources management, especially in determining the conditions of ungauged subbasins. The presence of missing data in weather input data can cause poor representation of the climate conditions in a catchment especially for large or mountainous catchments. Therefore, in this study, a custom module was developed and evaluated to determine the efficiency of utilizing basic spatial interpolation methods in the estimation of weather input data. The module has been written in Python language and can be considered as a pre-processing module prior to using the SWAT model. The results of this study suggests that the utilization of the proposed pre-processing module can improve the simulation results for both outflow and sediment yield in a catchment, even in the presence of missing data.

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Microencapsulation of Water-Soluble Isoflavone and Physico-Chemical Property in Milk

  • Seok, Jin-Seok;Kim, Jae-Soon;Kwak, Hae-Soo
    • Archives of Pharmacal Research
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    • v.26 no.5
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    • pp.426-431
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    • 2003
  • This study was carried out to investigate the addition of water-soluble isoflavone into milk by means of microencapsulation technique. The yield of microencapsulation, sensory attributes, and capsule stability of water-soluble isoflavone microcapsules in milk were measured. Coating materials used was polyglycerol monostearate (PGMS), and core material was water-soluble isoflavone. The encapsulation yield of water-soluble isoflavone with PGMS was 67.2% when the ratio of coating material to core material was 15 : 1. The rate of water-soluble isoflavone release from capsules was 18, 19, and 25% when stored at 4,20, and $30^{\circ}C$ for 12 days in milk, respectively. In sensory evaluation, beany flavor and color of microencapsuled water-soluble isoflavone added milk were significantly different from uncapsuled water-soluble isoflavone added milk, however, bitterness was not significantly different. In vitro study, micro-capsules of water-soluble isoflavone in simulated gastric fluid with the range of 3 to 6 pHs were released 3.0∼15.0%, however, the capsules in simulated intestinal fluid with pH 7 were released 95.7% for 40 min incubation time. In conclusion, this study provided that PGMS as coating materials was suitable for the microencapsulation of water-soluble isoflavone, and the capsule containing milk was almost not affected with sensory attribute.

Bending performance and calculation of reinforced beam with hybrid fiber and CaCO3 whisker

  • Li Li;Yapeng Qin;Mingli Cao;Junfeng Guan;Chaopeng Xie
    • Computers and Concrete
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    • v.31 no.3
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    • pp.197-206
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    • 2023
  • In this paper, the bending performance of a MSFRHPC (containing steel fiber, polyvinyl alcohol (PVA) fiber, and CW)-reinforced beam was studied for the first time. Introducing a multiscale fiber system increased the first crack load (up to 150%), yield load (up to 50%), and peak load (up to 15%) of reinforced beams. The multiscale fiber system delays cracking of the reinforced beam, reduces crack width of the reinforced beam in normal use, and improves the durability of the beam. Considering yield load and peak load, the reinforcing effect of multiscale fiber on the high-reinforcement ratio beam (1.00%) is better than that on the low-reinforcement ratio beam (0.57%). Introducing fibers slowed the development of cracks in the reinforced beam under bending. With the added hybrid fiber, the deformation concentration of reinforced beams after yield was more significant with concentration in 1 or 2 cracks. A model for predicting the flexural capacity of MSFRHPC-reinforced beams was proposed, considering the action of multiscale hybrid fibers. This research is helpful for structure application of MSFRHPC-containing CW.

Influence of Varying Level of Sodium Bicarbonate on Milk Yield and Its Composition in Early Lactating Nili Ravi Buffaloes

  • Sarwar, M.;Shahzad, M. Aasif;Nisa, Marhr-un
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.12
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    • pp.1858-1864
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    • 2007
  • Influence of varying level of sodium bicarbonate (SB) supplementation on milk yield and its composition was examined in a randomized complete block design in early lactating Nili Ravi buffaloes during summer. Four iso-nitrogenous and iso-caloric diets were formulated. The diet 0B contained 0 while LB, MB and HB diets contained 0.50, 1.0 and 1.50% SB levels, respectively. The diets were randomly allotted to twenty buffaloes, five in each group. A linear increase in nutrient and water intake was recorded with increasing SB level. Buffaloes fed MB and HB diets showed higher nitrogen balance than those fed 0B and LB diets. A significant increase in blood pH and serum bicarbonate was noticed with increasing SB level. Urine pH increased significantly with increased SB level. A linear increase in milk yield was also noticed with increasing SB level. Milk fat% increased significantly in buffaloes fed MB and HB diets compared with those fed 0B and LB diets. Buffaloes fed HB diet had higher conception rate and less services per conception than those fed 0B diet. This study indicated that a high SB diet not only increased dry matter and water intake, milk yield, milk fat% but also increased conception rate in early lactating buffaloes during summer.

Simulating Evapotranspiration and Yield Responses of Rice to Climate Change using FAO-AquaCrop (FAO-AquaCrop을 이용한 기후변화가 벼 증발산량 및 수확량에 미치는 영향 모의)

  • Chung, Sang-Ok
    • Journal of The Korean Society of Agricultural Engineers
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    • v.52 no.3
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    • pp.57-64
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    • 2010
  • The impacts of climate change on yield and evapotranspiration of rice have been modeled using AquaCrop model developed by Food and Agriculture Organization (FAO). Climate change scenario downscaled by Mesoscale Model 5 (MM5) regional model from ECHO-G General Circulation Model (GCM) outputs by Korea Meteorological Research Institute (METRI) was used in this study. Monthly average climate data for baseline (1971-2000) and three time periods (2020s, 2050s and 2080s) were used as inputs to the AquaCrop model. The results showed that the evapotranspiration after transplanting was projected to increase by 4 % (2020s), 8 % (2050s) and 14 % (2080s), respectively, from the baseline value of 464 mm. The potential rice yield was 6.4 t/ha and water productivity was 1.4 kg/$m^3$ for the baseline. The potential rice yield was projected to increase by 23 % (2020s), 55 % (2050s), and 98 % (2080s), respectively, by the increased photosynthesis along with the $CO_2$ concentration increases. The water productivity was projected to increase by 19 % (2020s), 44 % (2050s), and 75 % (2080s), respectively.

Sensitivity Analysis of the Groundwater Flow Model Parameters in a Small Rural Watershed (농촌 소유역에서 지하수 유동 모형의 매개변수 민감도 분석)

  • Park, Ki-Jung;Chung, Sang-Ok
    • Journal of Korea Water Resources Association
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    • v.37 no.8
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    • pp.687-693
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    • 2004
  • The MODFLOW simulated results with varying input parameter values were compared and analyzed. To understand the relative importance of the input parameters, sensitivity analysis was carried out. The amount of sustainable yield was analyzed with respect to the hydraulic conductivity, specific yield, specific storage, aquifer thickness and the distance of the wells from the river. The results of sensitivity analysis showed that inflow from the river and the aquifer storage were sensitive to the specific yield and aquifer thickness. Sustainable yield was sensitive to the hydraulic conductivity and aquifer thickness. The results of this study can be used as a basic information for groundwater development and management plannings considering regional characteristics.