• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.15 no.1
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• pp.25-35
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• 2010

The biodiversity of eukaryotic plankton has commonly been used to evaluate the status of aquatic ecosystems. Therefore, an accurate and rapid method for species identification is needed to reveal the biodiversity of environmental water samples. To date, molecular methods have provided a great deal of information that has enabled identification of the hidden biodiversity in environmental samples. In this study, we utilized environmental polymerase chain reaction (PCR) and constructed the 18S nuclear ribosomal RNA clone library from environmental water samples in order to develop more efficient methods for species identification. For the molecular analysis, water samples were collected from the Seonakdong River (Gimhae Bridge) and the coast of Namhae，(Namhaedo). Colony PCR and restriction fragment length polymorphism of PCR (PCR-RFLP) were then adopted to isolate unique clones from the 18S rDNA clone library. Restriction fragment length polymorphism pattern analysis of the Gimhae Bridge sample revealed 44 unique clones from a total of 60 randomly selected clones, while analysis of the Namhae sample revealed 27 unique clones from 150 clones selected at random. A BLAST search and subsequent phylogenetic analysis conducted using the sequences of these clones revealed hidden biodiversity containing a wide range of taxonomic groups (Heterokontophyta (7), Ciliophora (23), Dinophyta (1), Chytridiomycota (1), Rotifera (1) and Arthropoda (11) in the Gimhae Bridge samples Ciliophora (4), Dinophyta (3), Cryptophyta (1), Arthropoda (19) in the Namhae samples). Therefore, the molecular monitoring method developed here can provide additional information regarding the biodiversity and community structure of eukaryotic plankton in environmental samples and helps construct a useful database of biodiversity for aquatic ecosystems.

• Journal of Wetlands Research
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• v.18 no.3
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• pp.218-225
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• 2016

Various strategies and management plans have been established in order to conserve water quality as well as ecosystem, prevent deterioration of scenic area, and more importantly, retain drinking water securely. However, due to the introduction of numerous small-scale development projects on the waterside area outside protection area of source water and reparian area, river water quality and landscape are severely deteriorated. In this study, We analyzed the expected environmental impacts on the ecosystem, topograph, geology, landscape, water quality according to development type, and assigned different environmental points to each development type depend on environmental impacts, which is able to divide small-scale development project into three categories such as projects with rigorous review, projects with general review and projects with simple review. Finally, we suggested the appraisal basement is to avoid or minimize the expected environment impacts of the small-scale development projects in riparian areas so that make them sustainable development by reasonable restriction.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.38 no.3
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• pp.172-183
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• 2005

To assess the macrobenthic community of oligohaline Youngam Lake, which is located at the Youngsan Watershed on the southwest part of Korea, macrobenthic fauna were collected at 45 stations during May, 2002. A total of 16 species of macrofauna were recorded with a mean density of 240 individuals per $m^2$ and a mean biomass of 7.07 g wet weight per $m^2$. Major dominant faunal groups were crustacean arthropods in terms of the number of species and abundance, and polychaete annelids in terms of biomass. The mean grain size was $5.7\;{\phi}$ which was dominated by silt fraction. The hydrological environment of the lake was characterised as an oligohaline environment with a mean surface water temperature of $17.8^{\circ}C$ and a mean salinity of 2.08 psu. The major dominant species were amphipods, Corophium sp. ($31\%$) and Jesogammarus sp. ($25\%$). Lowe. values of species diversity (H') with a mean of 0.81 (less than 1.0 from most stations) reflected the overall poor faunal diversity in this area. Multivariate analysis suggested that this benthic faunal community could be divided into four sub-regions such as the area from lake proper to water channel to the south, the stations located at the entrance and northern water channel, the stations near the dike, and the lake proper area. Freshwater and brackish water species which occurred in each station group were corresponded to the oligohaline salinity regime. Bottom hypoxia appeared in the entrance part of the lake between dyke and lake proper on May, which was resulted from stratification from spring season. These facts imply that marine macrobenthos were severely impacted by low salinity and a consequent hypoxia after embankment of the lake due to the restriction of water circulation.

• Structural Engineering and Mechanics
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• v.62 no.2
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• pp.209-224
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• 2017

Elevated water tanks are considered as important structures due to its post-earthquake requirements. Elevated water tank on reinforced concrete frame staging is widely used in India. Different response reduction factors depending on ductility of frame members are used in seismic design of frame staging. The study on appropriateness of response reduction factor for reinforced concrete tank staging is sparse in literature. In the present paper a systematic study on estimation of key components of response reduction factors is presented. By considering the various combinations of tank capacity, height of staging, seismic design level and design response reduction factors, forty-eight analytical models are developed and designed using relevant Indian codes. The minimum specified design cross section of column as per Indian code is found to be sufficient to accommodate the design steel. The strength factor and ductility factor are estimated using results of nonlinear static pushover analysis. It was observed that for seismic design category 'high' the strength factor has lesser contribution than ductility factor, whereas, opposite trend is observed for seismic design category 'low'. Further, the effects of staging height and tank capacity on strength and ductility factors for two different seismic design categories are studied. For both seismic design categories, the response reduction factors obtained from the nonlinear static analysis is higher than the code specified response reduction factors. The minimum dimension restriction of column is observed as key parameter in achieving the desired performance of the elevated water tank on frame staging.

• Journal of Korean Medical classics
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• v.22 no.4
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• pp.55-66
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• 2009

The formative procedure of five-viscera has been explained for the restriction[相克; 己勝] and the reverse restriction[相侮, 勝己] in five phase theory on the medical literatures from ancient times. In the formative procedure of five-viscera, it would seem that two theories are mutually contradictory. But this problem is solved in the view: As the upward, downward movement and the circulation progress, five-viscera and five-bowels develop. In brief on the formative procedure of human viscera and bowels, first, the circulation of five-viscera and five-bowels forms three phase of upper-middle-lower(fire-earth-water), and these phases generate Triple energizers[Samcho, 三焦], consequently it is to be five-viscera and six-bowels, to be formed 'Meridian and collateral[Gyeongnak, 經絡]' in that order. The triple energizers is situated in middle stage of formation of 'Vicera' and 'Meridian and collateral' on developing stage. It would be thought that Triple energizer meridian[Susoyangsamchogyeong, 手少陽三焦經] is formed in procedure of Meridian and collateral after formation of upper-middle-lower of the triple energizers.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.26 no.2
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• pp.137-145
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• 2000

Carcinogenesis is a multi-stage process that generally consists of at least three steps; initiation, promotion, and progression. If one of these carcinogenic steps were suppressed or delayed, the cancer could be prevented. Cancer chemoprevention is defined to be inhibition or reversal of the carcinogenic process by the specific chemical agents and is a novel approach to cancer management alternative to conventional chemotherapy. Chlorophylln(CHL), a water-soluble derivative of chlorophyll, containing sodium and copper, has been known to be strong antimutagen in several test systems, but its mechanism of antimutagenic action is unknown. In the present experiment, the possibility of CHL as chemopreventive drugs on 7,12-dimethylbenz[a]anthracene(DMBA)-induced hamster buccal pouch carcinogenesis was investigated by mutagenicity test, carcinogenicity test, and frequency or spectrum of H-ras mutations in the both of DMBA-induced and chlorophylln-pretreated-DMBA induced tumor by polymerase chain reaction and non-isotopic restriction fragment length polymorphism. The treatment of CHL reduced the yields and multiplicity of the 0.5% DMBA-induced tumor, 86% to 62.5% and $3.7{\pm}0.6$ to $1.4{\pm}0.3$, respectively. The occurrence of histidine revertant by $20{\mu}mole$ DMBA was inhibited 25.6 to 81.7% by 1 to $5{\mu}M$ CHL in a dose-dependent manner. The mutation rates of H-ras gene in DMBA-induced and CHL-pretreated-DMBA induced tumor were 96%, 94% of which the most mutations were in codon 12/13. These results suggest that CHL inhibits the carcinogenic action of DMBA by the formation of complex between CHL and DMBA or the inhibition of the activation of DMBA in vivo. But CHL did not affect the mutation rates or its spectrum in already formed tumor.

• Journal of Ecology and Environment
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• v.41 no.3
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• pp.99-106
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• 2017

Background: Plants over-expressing Arabidopsis ABF3 (abscisic acid-responsive element-binding factor 3) have enhanced tolerance to various environmental stresses, especially drought. Using terminal restriction fragment length polymorphism (T-RFLP) analysis, we compared the rhizosphere-associated structures of microbial communities for transgenic potato containing this gene and conventional "Jopoong" plants. Results: During a 2-year field experiment, fungal richness, evenness, and diversity varied by year, increasing in 2010 when a moderate water deficit occurred. By contrast, the bacterial richness decreased in 2010 while evenness and diversity were similar in both years. No significant difference was observed in any indices for either sampling time or plant line. Although the composition of the microbial communities (defined as T-RF profiles) changed according to year and sampling time, differences were not significant between the transgenic and control plants. Conclusions: The results in this study suggest that the insertion of ABF3 into potato has no detectable (by current T-RFLP technique) effects on rhizosphere communities, and that any possible influences, if any, can be masked by seasonal or yearly variations.

• Asian-Australasian Journal of Animal Sciences
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• v.4 no.1
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• pp.15-19
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• 1991

As a possible step to evaluate in the mammary gland the role of motherwort (Leonurus sibiricus L), a representative medicinal plant used traditionally for the therapy of gynecologic diseases, the effects of the agent on lactation which was suppressed by preganacy-dependent mammary tumors (PDMT) were studied in GR/A mice. Beginning the day of placing with males at 45-50 days of age, female mice were given 60% methanol-extract of the aerial part of motherwort as drinking water at the concentration of 0.5% throughout the experiment. Mice developing PDMT during pregnancy [PDMT(+)] and given motherwort were similar to mice developing no PDMT [PDMT(_)] with or without motherwort treatment and were significantly higher than PDMT(+) mice given tap water in litter growth and mammary RNA/DMA ratio on day 12 of the 2nd lactation. Mammary DNA and RNA contents were also elevated by motherwort in PDMT(+) mice. The results suggest that motherwort can ameliorate lactation suppressed by PDMT through its stimulation of both growth and function of the mammary glands.

• Proceedings of the Korea Water Resources Association Conference
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• 2007.05a
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• pp.1263-1267
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• 2007

A more complete two-dimensional vertical numerical model has been developed to describe the saltwater intrusion in an estuary. The model is based on the previous studies in order to obtain a better accuracy. The non-linear terms of the governing equations are analyzed and the $\sigma$-coordinate system is employed in the vertical direction with full transformation which is recently issued in several studies because numerical errors can be generated during the coordinate transformation of the diffusion term. The advection terms of the governing equations are discretized by an upwind scheme in second-order of accuracy. By employing an explicit scheme for the longitudinal direction and an implicit scheme for the vertical direction, the numerical model is free from the restriction of temporal step size caused by a relatively small grid ratio. In previous researches, some terms induced from the transformation have been intentionally excluded since they are asked the complicate discretization of the numerical model. However, the lack of these terms introduces significant errors during the numerical simulation of scalar transport problems, such as saltwater intrusion and sediment transport in an estuary. The numerical accuracy attributable to the full transformation is verified by comparing results with a previous model in a simply sloped topography. The numerical model is applied to the Han River estuary. Very reasonable agreements for salinity intrusion are observed.

• Transactions of the Korean Society of Automotive Engineers
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• v.24 no.1
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• pp.10-15
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• 2016

This research was conducted structural analysis in order to analyze the impact of the gear in Urea pump when the water is frozen. Subject of study, gear was designed nine models, this gear is a spur gear, located in pump. Contact conditions and rotation conditions were set the gear's condition of restriction. Given 136 MPa pressure to external gear by water was set to the applied stress. The performing result of structural analysis, maximum stress and strain are appeared between two gears. At the same diameter, strain and stress are decreased gradually thicker. Because of the little part in crevice between gears, this parts of gears could be obtained conclusion to be generated maximum stress and strain.