• Journal of Species Research
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• v.7 no.1
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• pp.50-59
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• 2018

During the course of investigation of indigenous prokaryotic species in Korea, a total of 30 bacterial strains belonging to the phylum Firmicutes were isolated from diverse environmental sites such as soil, avian feces, wastewater treatment plants, fermented vegetables, seawater, algae, sea cucumber, octopus and tidal flat sediment. Phylogenetic analysis based on 16S rRNA gene sequences revealed that each strain showed high sequence similarity (${\geq}98.7%$) to the closest type strain and formed a robust phylogenetic clade with the most closely related species in the phylum Firmicutes. To date, there is no official record of these 30 species in Korea. Therefore, we report 26 species of 12 genera in the order Bacillales and 4 species of 4 genera in the order Lactobacillales which have not been reported in Korea. Morphological and biochemical characteristics, isolation sources and NIBR deposit numbers are described in the species descriptions.

• Journal of the Korea Organic Resources Recycling Association
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• v.24 no.3
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• pp.101-108
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• 2016

Concentrations of Chromium(Cr) and Manganese(Mn) in several kinds of dewatered organic sewage sludges generated from wastewater treatment plants of Pocheon City, Gyeongii Province were examined. In addition, bioaccumulations of Cr and Mn in the body of earthworm Eisenia fetida were also investigated by changing of feed sludge amounts. Cr and Mn concentrations in sludges were in the range of 0.0~0.3 mg/kg and 3.6~17.6 mg/kg, respectively, which would not cause acute toxicities to earthworm population. Cr was bioaccumulated in the earthworm body but bioaccumulation was not proportional to the amount of sludges supplied. The degree of bioaccumulation of Cr decreased in later phase as the supplied amounts of sludges increased. Meanwhile, there were rare bioaccumulation with regard to Mn. In this study, the value ranges of bioaccumulation factor (BAF) for Mn were 0.00~0.12 when 60g of sludges was supplied. In the case of Cr, the value of BAF was 19.33 when 60g of human manure sludge was provided.

• Journal of Korean Society on Water Environment
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• v.33 no.1
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• pp.51-62
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• 2017

The purpose of this study is to evaluate on the applicability of Load Duration Curve (LDC) method using Maintenance of Variance Extension types 2 method and sampling data for efficient total maximum daily loads at the Nakbon-A unit watershed in Korea. The LDC method allows for characterizing water quality data such as BOD, TOC, T-N and T-P in this study at different flow regimes(or quarters). BOD usually exceeded the standard value (exceedance probability 50%) at low flow zone. On the other hand, TOC, T-N, T-P usually exceeded the standard value at dry and low flow zone. Seasonally all water quality variables usually exceeded the standard value at Q1(Jan-Mar) and Q2(Apr-Jun) zones. Improvement of effluent control from wastewater treatment plants are effective to improve BOD and T-P.

• Journal of Korean Society on Water Environment
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• v.33 no.6
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• pp.696-714
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• 2017

Simple material budget models were developed to predict the spring season (March ~ May) water quality for a river-type reservoir Paldang, in the Republic of Korea. These models are available at mixed water bodies whose light intensity is negligible at the bottom. The calculated data from the models fit quite well with field data collected for 30 years, from 1988 to 2017. The apparent settling velocity of total phosphorus was estimated to be $110m\;d^{-1}$. The critical hydraulic load that determines the usability of phosphorus for algal production appeared to be about $2.0m\;d^{-1}$. When a hydraulic load was larger than the critical value, the concentrations of chlorophyll ${\alpha}$ ($Chl.{\alpha}$), chemical oxygen demand (COD), and 5-day biochemical oxygen demand BOD in the reservoir water became insensitive to internal algal reactions. The model analysis showed that the allochthonous COD continued to increase while the allochthonous BOD slightly decreased after 1999. The decrease of allochthonous BOD is due to the expansion of sewage and wastewater treatment plants in the watershed. The increase of allochthonous COD seems to result from the increase in anthropogenic non-point sources as well as the increase in the discharge of natural organic matters due to climate change. Organic matter of algal origin continued to increase until the mid-2000s, but recently it has decreased as the phosphorus concentration has decreased. The COD and BOD of algal origin increased from 35 % and 27 % during 1988 ~ 1994 to 43 % and 40 % during 2000 ~ 2010, respectively, and then decreased to 25 % and 28 % during 2011 ~ 2017.

• Journal of Korean Society on Water Environment
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• v.24 no.1
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• pp.19-29
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• 2008

Hot air sparging is a groundwater remediation technique, in which organic contaminants volatilized into hot air from the saturated to vadose zone. In the laboratory diesel (10,000 mg TPH/kg) was spiked in contaminated saturated aquifer soil. The hot air ($34.9{\pm}2.7^{\circ}C$) was injected in intermittent (Q=1,500 mL/min, 10 minute injection and 10 minute idle) modes. We performed microcosm tests using the groundwater samples to assess TPH reductive remediation activity. For Terminal-Restriction Fragment Length Polymorphism (T-RFLP) analysis of eubacterial communities in sludge of wastewater treatment plants and soil of experiment site, the 16S rDNA was amplified by Polymerase Chain Reaction (PCR) from the sludge and the soil. The obtained 16S rDNA fragments were digested with Msp I and separated by electrophoresis gel. We found various sequence types for hot air sparging experiment with sludge soil samples that were closely related to Bacillus (149 bp, Firmicutes), Methlobacterium (149 bp, Euryarchaeotes), Pseudomonas (492 bp, ${\gamma}$-Proteobacteria), etc., in the clone library. In this study we find that TPH-water was reduced to 78.9% of the initial value in this experiment aquifer. The results of the present study suggests that T-RFLP method may be applied as a useful tool for the monitoring in the TPH contaminated soil fate of microorganisms in natural microbial community.

• Ecology and Resilient Infrastructure
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• v.6 no.2
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• pp.128-135
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• 2019

This study evaluates the effect of dissolved effluent organic matter (SE-dEfOM) from sewage wastewater treatment plants on the adsorption and estrogenic activity of bisphenol A (BPA). Specific ultraviolet absorbance and fluorescence index analyses indicated that SE-dEfOM was mainly microbially derived non-humic substances differed from Suwannee River natural organic matter (SR-NOM) as reference. Both Langmuir and Freundlich models successfully explained the adsorption of BPA onto both SE-dEfOM and SR-NOM. Additionally, the SE-dEfOM showed higher binding capacities and affinities for BPA than those of SR-NOM, resulting in better reduction of the estrogenic activity of BPA. These findings suggest that the binding and toxicity of BPA are largely dependent on the source of organic matters.

• Journal of the Earthquake Engineering Society of Korea
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• v.27 no.4
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• pp.163-170
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• 2023

In the fluid-structure interaction analysis, the finite element formulation is performed for the wave equation for dynamic fluid pressure, and the dynamic pressure is defined as a degree of freedom at the fluid nodes. Therefore, to connect the fluid to the structure, it is necessary to connect the degree of freedom of fluid dynamic pressure and the degree of freedom of structure displacement through an interface element derived from the relationship between dynamic pressure and displacement. The previously proposed fluid-structure interface elements use conformal finite element meshes in which the fluid and structure match. However, it is challenging to construct conformal meshes when complex models, such as water purification plants and wastewater treatment facilities, are models. Therefore, to increase modeling convenience, a method is required to model the fluid and structure domains by independent finite element meshes and then connect them. In this study, two fluid-structure interface elements, one based on constraints and the other based on the integration of nonsmooth functions, are proposed in nonconformal finite element meshes for structures and fluids, and their accuracy is verified.

• Korean Journal of Ecology and Environment
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• v.36 no.3 s.104
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• pp.356-368
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• 2003

Water quality of effluent from wastewater treatment plants (WWTPS) was reviewed to examine the feasibility of agricultural reuse using USEPA and WHO guidelines. It might meet the guidelines for BOD and SS, however, the most critical microbiological concentration was too high and further treatment is required. The pilot study of three treatments were performed to reduce microbiological concentrations. The UV irradiation was proved to be very effective in disinfection of secondary level effluent, and about 30 mW ${\cdot}$ s/$cm^2$ of dose was suggested to meet the even most stringent USEPA guidelines. Slow sand filter demonstrated effective removal of bacteria, and effluent concentration of total coliform (TC), fecal coliform (FC), and E. coli. dropped from about 10,000/100 mL to 300, 200, and 150 MPN/100 mL, respectively, showing over 95% removal. These level of bacterial concentration sufficiently meet the WHO guidelines ($10^3\;{\sim}\;10^5$ FC/100 mL), and could meet the more stringent USEPA guidelines (200 FC/100 mL) if properly applied. Slow sand filter also provided about 50% removal of SS, turbidity, and BOD in addition to bacterial removal. The removal efficiency of pond system was relatively poor, but still showed over 85% removal and effluent concentration of TC, FC, and E. coli was all below 10,000/100 mL. The pond system alone could meet the WHO guidelines, but hardly meet the USEPA guidelines and further treatment might be necessary. Overall, three methods evaluated in the study treat the effluent to meet the WHO microbiological guidelines for agricultural reuse. The UV disinfection and slow sand filter might also could the USEPA guidelines, while the pond system can hardly meet the USEPA guidelines if applied alone. The WHO and USEPA guidelines were made based on data from upland field agricultural system and may not be directly applicable to the paddy field agricultural system. Therefore, national standards for agricultural reuse of reclaimed water should be made considering domestic agricultural systems as well as international guidelines. Also, further investigation is recommended to develop optimum and feasible treatment measures for agricultural reuse of effluent from WWTPs.

• Korean Journal of Environmental Agriculture
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• v.21 no.3
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• pp.178-188
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• 2002

Constructed wetlands typically cost less to build and operate, and require less energy than standard mechanical treatment technology but they have similar performance to centralized wastewater treatment plants. Therefore, they were constructed especially many in rural areas, where are small villages but not industries. Plantless column tests were performed to investigate the possibility on using zeolite as a filter medium of constructed wetland for the wastewater treatment. Removal efficiency was $COD_{Cr}$ 94.63% T-P 41.41% and $NH_4^+-N$ 99.75% at hydraulic load 314 $L/m^2{\cdot}d$ and filtering height 100 cm filled with a zeolite mixture. This zeolite mixture consisted of 1 : 1 by volume of zeolites in the diameter range of 0.5 to 1 mm and 1 to 3 mm. Accordingly, hydraulic load 314 $L/m^2{\cdot}d$ was considered as optimal. Three zeolite mixtures were used to determine the optimal mixing ratio by volume of a zeolite (A) in the diameter range of 0.5 to 1 mm to a zeolite (B) in the diameter range of 1 to 3 mm 1 : 3, 1 : 1 and only B in A to B by volume were tested at hydraulic load 314 $L/m^2{\cdot}d$ and filtering height 100 cm $COD_{Cr}$ removal efficiency was more than 89% at mixing ratios of 1 : 3 and 1 : 1 in A to B. That of T-P ranged 56.42 to 58.72% and, that of T-N and $NH_4^+-N$ was 87% and 99% regardless of mixing ratios of two zeolites. Removal efficiency was lower at the column filled with only B. Removal efficiency was better at Inter medium filled with mixing ratio 1 : 1 in A to B than with the other mixing ratios. Thus, it was found that the mixture of mixing ratio 1 : 1 in A to B was appropriate far Inter medium of constructed wetland Removal efficiency was higher in down-flow than in up-flow, and all contaminants were removed most in 20 cm filter height near feeding area.

• Korean Journal of Ecology and Environment
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• v.39 no.1 s.115
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• pp.62-72
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• 2006

Biological health in aquatic environments has been assessed by several approaches using various bio-taxa. Especially, fish has been identified as one of the best ecological indicators for an integrative health assessments in aquatic environments. In this preliminary study, the necropsy-based approach using the Health Assessment Index (Adams et al., 1993; HAI, Blazer, 2000), was employed for the assessments of aquatic environments influenced by effluent water from wastewater disposal plants (WDP) near a urban stream (i.e., Cap-Stream). For the impact analysis from the effluents, we developed 10 metric necropsy-based model including $M_l\;skin,\;M_2\;fin,\;M_3\;thymus,\;M_4\;spleen,\;M_5\;hindgut,\;M_6\;kidney,\;M_7\;liver.\;M_8\;eyes,\;M_9\;gill,\;and\;M_{10}$ pseudobranch. We set up duplicate controls (natural upstream water plus 5 fishes in each control tank) and duplicate treatments (effluent water from the WDP plus 5 fishes in each treatment tank) in the laboratory. Preliminary results showed that HAI values in the controls were zero, indicating an excellent condition by the criteria of HAI, while the HAI values in the treatments ranged between 40 and 150, indicating a poor condition. Also, various health conditions, based on the index of biological integrity (IBI), species richness, tolerance guilds, and trophic guilds supported the HAI approach. The HAI approach, not applied in Korea, not may be used as an surrogate fer ecological health assessment in lotic ecosystems.