• Journal of Korean Society of Environmental Engineers
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• v.39 no.3
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• pp.155-163
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• 2017

Membrane filtration has become more popular in drinking water treatment recently, since the filtration can control not only particulate matters but also pathogenic microorganisms such as giardia and cryptosporidium very effectively. Pilot-scale ($120m^3/d$ of treatment capacity) and test-bed ($25,000m^3/d$ of treatment capacity) microfiltration experiments were conducted to find optimum operating mode and the critical flux. Optimum operating mode of pilot-test was assessed as inflow 1.0 min, filtration 36.5 min, air backwash 0.9 min, backwash 1.0 min and outflow 1.0 min with 50 LMH ($L/min{\cdot}m3^$) of critical flux. Critical Flux was calculated to be $50L/m^2-h$ (within TMP 0.5 bar) based on the increase formula of the transmembrane pressure difference according to the change of time at Flux 20, 40, 56 and 62 LMH in pilot operation. Chemical cleaning was first acid washed twice, and alkali washing was performed secondarily, and a recovery rate of 95% was obtained in the test-bed plant. The results of operating under these appropriate conditions are as follows. Turbidity of treated water were 0.028, 0.024, 0.026 and 0.028 NTU in spring, summer, autumn and winter time, respectively. Microfiltration has superior treatment capability and performance characteristics in removing suspended solids and colloidal materials, which are the main cause of turbidity and important carrier of metal elements, and it has shown great potential in being an economically substitute to traditional processes (sand filtration).

• Journal of the Korean Home Economics Association
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• v.41 no.10 s.188
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• pp.101-116
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• 2003

The study was conducted to establish hygienic standards of salad items for pizza restaurant located in Seoul by applying HACCP system during the summer of 2000. The study measured temperature, time, pH, Aw and microbial assessments. The hygienic conditions of the kitchen and workers were on the average(1.21, 1.0 out of 3 pts.), but some improvement should be made: separate use of trash can and leftover disposal, separate use of knives and cutting boards, habits for hand washing and wearing hygienic gloves. For salad production, all procedures were peformed under food safety danger zone ($5{\~}60^{\circ}C$). The ingredients were mostly above pH 5.0 and high in Aw($0.94{\~}0.99$). Microbial assessments for salad production revealed that TPC($1.8{\times}10^3{\~}1.0{\times}10^{10}CFU/g$) and coliforms($1.5{\times}10{\~}5.2{\times}10^5 CFU/g$) exceeded the standards by Solberg et al.(TPC: $10^6CFU/g$, coliforms: $10^3CFU/g$). S. aureus was not detected but Salmonella was found in three food items(egg, macaroni and macaroni salad). Moreover, the workers' hands contained 3.1 104 CFU/g of TPC and 4.2 102 CFU/g of S. aureus requiring further remedy since it exceeded the safety standards suggested by Harrigan and McCance (500 CFU/g of TPC per $100cm^2$ and 10 CFU/g of coliforms per $100cm^2$). According to the critical control point(CCP) decision tree analysis, vegetable receiving, vegetable holding, mixing, display on coleslaw, macaroni draining, display on macaroni salad, egg peeling & cutting, apple cutting, and display on salad bar were determined as CCPs. From the findings it would be suggested that purchase of Quality materials, short holding and display time, storing food at right temperature, using sanitary cooking utensils, and improvement of workers' food handing practices are needed to ensure the safe salad production in this specific pizza restaurant.

• Reproductive and Developmental Biology
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• v.35 no.1
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• pp.61-65
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• 2011

The objective of this study was to examine the effect of various discontinuous Percoll washing conditions on motile sperm recovery rate and motion kinematics. Frozen semen samples from 3 bulls (0.5 ml plastic straws, 6% glycerol in egg yolk-Tris-glycerol extender) were thawed in $37^{\circ}C$ water bath for 1 min. After thawing, the mixed semen samples were randomly allocated to 12 treatment groups. Briefly, the spermatozoa were centrifuged for three different time lengths (10, 20, and 30 min) at two gravities ($300{\times}g$ and $700{\times}g$) through two concentrations of discontinuous Percoll density gradient of 1 ml 90%: 1 ml 45% Percoll and 2 ml 90%: 2 ml 45% Percoll to remove extender, debris, and dead spermatozoa. Motile sperm recovery rate and motion kinematics were evaluated by computer assisted sperm analyzer using Makler counting chamber. Sperm motility (%) and motile sperm recovery rate showed similar pattern in all treatment groups. However, sperm motility (%) and motile sperm recovery rate were highest at $700{\times}g$ for 30 min through a discontionous Percoll density gradient of 1 ml 90%: 1 ml 45% Percoll. There were no significant differences in motion kinematics after various Percoll washings. These results suggest that force of centrifugation, centrifugation time, and Percoll volume significantly affect motile sperm recovery rate.

• Journal of Korean Ophthalmic Optics Society
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• v.18 no.2
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• pp.107-115
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• 2013

Purpose: This study was conducted to investigate the change of soft contact lens parameters after eyeliner deposition and compare the cleaning efficiency and the recovery rate of lens parameters by different cleaning methods. Methods: The eyeliner was deposited on three types of soft contact lenses made of different materials for 1 hour, 4 hours and 6 hours and those lenses were washed by the eyeglasses ultrasonic cleaning and by hands. The changes in visible light transmittance, diameter and surface of those lenses were compared before and after cleaning of the lens. Results: The visible light transmittance of soft contact lenses decreased along with the deposition time of eyeliner. The patterns of the eyeliner deposition on soft contact lens appeared differently depending on the physical properties of the lens material. Thus, the largest eyeliner deposition was found on narafilcon A lens. The diameters of hilafilcon B and etafilcon A lenses were decreased, however, the diameter of narafilcon A lens was increased. The cleaning efficiency of the eyeliner deposition was greater with the multipurpose solution by hands than with the eyeglasses ultrasonic cleaner. However, the recovery of lens parameters such as the lens diameter, visible light transmittance and lens surface due to the eyeliner deposition to its original state was limited. Conclusions: From these results, it is suggested to minimize eye makeup when wearing soft contact lens. Furthermore, the reduced wearing time and shorter cycle of lens replacement may be needed by considering a change of lens parameter when the repeat eye makeup is applied.

• Korean Journal of Medicinal Crop Science
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• v.16 no.5
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• pp.320-325
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• 2008

Machilus thunbergii has been showed relation to antimicrobial activity on minimal inhibitoty concentration (MIC) and colony forming inhibitory activity (CFIA) test, so that can be used to food preservatives for green vegetable. Nanoparticles has been made of edible materials. 80% of the nanoparticles has been characterized by image analyser and electron microscopy, showing in the range under 300 nm diameter. The sprayed nanoparticles remained on the surface of chicon even after washing by dilution water, then activate biological activities for storage of chicon with storing and releasement system of extracts. Chicon treated nanoparticle has been kept fresh condition about 2 months longer than 3 weeks of the non-treated control. It can be tell that treatment with nanoparticle of M. thunbergii extracts extends storage time of chicon possibly by inhibition of ethylene production through efficiency control on cell breathing.

• Journal of Yeungnam Medical Science
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• v.29 no.2
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• pp.83-88
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• 2012

Background: This study was conducted to evaluate the usefulness of the BACTEC MGIT (Mycobacterium Growth Indicator Tube) 960 system for mycobacteria culture and immunochromatographic assay to identify Mycobacterium tuberculosis (MTB) in positive MGIT culture. Methods: Mycobacteria-culture-positive cases were retrospectively analyzed from December 2010 to July 2011. The detection rates and the recovery times of the mycobacteria between the Ogawa media and the MGIT were compared. An immunochromatographic assay (ICA) (SD BIO-LINE) was also performed in the positive MGIT culture for identification, and the results were compared with those of the Ogawa media in the Korea National Tuberculosis Association. Results: Among the 261 patients (M:F, 168:93; mean age, $61.6{\pm}17.16$ yrs), 450 specimens (sputa, 365; bronchial washing, 61; and pleural effusion, 24) were found positive with mycobacteria. Mycobacteria were grown both on the MGIT and Ogawa media in 310 cases (68.9%); only on the MGIT in 115 cases (22.6%); and only on the Ogawa media in 25 cases (5.5%) (p<0.05). The recovery time was $28.2{\pm}8.9$ days in the Ogawa media and $11.1{\pm}5.8$ days in the MGIT (p<0.05). Among the 127 cases from the positive MGIT culture, all 92 cases that were confirmed as MTB cases bythe Korea National Tuberculosis Association were identified as MTB by ICA, with 100% sensitivity. Conclusion: MGIT increases the detection rate and shortens the recovery time of mycobacteria in clinical respiratory specimens, and the TB Ag MPT64 kit using ICA is useful in identifying MTB in a positive MGIT culture.

• Membrane Journal
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• v.18 no.1
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• pp.7-25
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• 2008

Sodium dodecyl sulfate, which was anionic surfactant, at a concentration higher than its critical micellar concentration was added to calcium solution for forming micelles. Then aggregates were formed by adsorption or binding of calcium ions on the surface of micelles, and gathering between them, and then rejected by two kinds of ceramic membranes to remove calcium ions. As result, rejection rates of calcium were higher than 99.98%. And in our experimental range the higher TMP trended to increase the resistance of membrane fouling ($R_f$), total permeate volume ($V_T$), dimensionless permeate flux ($J/J_o$) and permeate flux (J) because TMP was driving force. And we investigated effects of $N_2$-back-flushing time and filtration time, that was back-flushing period, during periodic $N_2$-back-flushing on ceramic membranes. As result, optimal BTs for NCMT-623l ($0.07{\mu}m$ pore size) and NCMT-7231 membrane ($0.10{\mu}m$) were 10 sec and 15 sec, respectively. Also, optimal FT was 5 min for both membranes, and the frequent $N_2$-back-flushing could decrease membrane fouling effectively. Then, the optimal conditions resulting from our experiments for synthetic calcium solution were applied to groundwater using as washing process of soymilk package. As result, rejection rates of calcium were higher than 99.98%.

• Journal of Korea Soil Environment Society
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• v.4 no.2
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• pp.63-75
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• 1999

This study was performed to investigate applicabilities of sewage sludge and papermill sludge as carbon sources in biologically treating abandoned acidic mine drainage, and effects of limestone. In spite of ranging average 3.3 in influent pH, SRB(Sulfate Reducing Bacteria) was well grown. because effluent pH was maintained by alkalinity a little under for whole stages. TCODcr was high in effluent with washing out in early stage. but its concentration was low with passed time and did not cause the problem of secondary pollution. Removal rate of Mn was remarkably low, but in case of heavy metals such as Fe, the fixation trends showed high as the volume of ${SO_4}^2$ reduction increased. In case of mixing sewage sludge and papermill sludge, when their mixing ratio were 1 : 1 and 2 : 1 respectively, rate of ${SO_4}^2$ reduction and removal rate of heavy metals were high. The mixing ratio 1 : 1 was considered as the most appropriate, because degradability of swewage sludge under short time was higher than that of papermill sludge. As a result of investigating efficiencies of limestone and biological treatment, biological treatment excelled limestone in neutralization and removal rate of heavy metals.

• Journal of Food Hygiene and Safety
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• v.22 no.1
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• pp.63-75
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• 2007

A survey on sanitary management at 98 food service institutions located in Jeollabuk-Do were conducted. Food service institutions included 13 hospitals, 38 schools, 40 enterprises, and 7 others. The sterilization in boiling water was used for disinfection of tableware, spoon and chopsticks, and dish towel. Cooking kit, refrigerator, chopping board, and knife were sterilized with disinfectant. Frequency of sanitary training program on employees was 56.1% for 1 time/month, 12.2% for 2-3 times/month, and 11.3% for 1 time/week. Preparing ratio of defrosting, warehouse and rest room, and washing and sterilization diary were 73.8, 60.5, and 54.5%, respectively. However, checking ratio of environments (9.5%), personal sanitation (10.8%), and sanitary training program diary(8.4%) were low. Major obstacle factors on the introduction of Hazard analysis critical control point (HACCP) system into food service institutions were lack of equipment and facilities (54.1%) and lack of understanding and data on HACCP system (17.3%). Therefore, in order to reduce the outbreak of food-borne diseases increasing frequency, we estimated that the introduction of sanitary management system and the conversion of dietician' perception on sanitary managements in food service institutions were needed.

• Journal of Korean Society on Water Environment
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• v.31 no.5
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• pp.523-532
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• 2015

Hydrocyclone is widely used in industry, for its simple design, high capacity, low maintenance and low operational cost. The objective of this study is to develop hydrocyclone coagulation and filtration system. The system is made of hydrocyclone ballasted coagulation with polyaluminium chloride silicate (PACS) and upflow filter to treat micro particles in urban storm runoff. Roadside sediment particles (< $200{\mu}m$) was mixed with tap water to make various turbid suspensions to simulate urban storm runoff. The filter cartridge was filled with polyethylene media system and ran 1hr per everyday and total operation time were 8.19hrs and backwashing everyday after end of operation. The operation condition of flowrate was $8.2{\sim}11.9m^3/day$ (mean $10.1m^3/day$) and surface overflow rate (SOR) based on filter surface area was $45.5{\sim}65.9m^3/m^2/day$ (mean $55.7m^3/m^2/day$). The range of PACS dosage concentration was 14.0~31.5 mg/L. As the results of operation, the range of removal efficiency of turbidity, SS were 81.0~95.8% (mean 89.5%) 81.8~99.0% (mean 91.4%), respectively. An increase of filtration basin retention time brought on increased of removal efficiency of turbidity and SS, and increase of SOR brought on decreased of removal efficiency. During the first flush in urban area, storm runoff have an high concentration of SS (200~600 mg/L) and the filtration bed becomes clogged and decreased of removal efficiency. Backwashing begins when the drainage pipe valve at the filtration tank bottom is completely open (backwashing stage 1). Backwashing stage 2 was using air bubbles and water jet washing the media for 5 mins and open the drainage valve. After backwashing stage 1, 2, 61.83~64.04%, 18.53~27.51% of SS loading was discharged from filtration tank, respectively. Discharged SS loading from effluent was 7.12~14.79% and the range of residual SS loading in fliter was 2.26~5.00%. The backwashing effects for turbidity, SS were 89.5%, 91.4%, respectively. The hydrocyclone coagulation and filtration with backwashing system, which came out to solve the problems of the costly exchange filter media, and low efficiency of removing micro particles of filter type nonpoint treatment devices, is considered as an alternative system.