• Title/Summary/Keyword: Washing Media

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A Study on the Compensation Algorithm for Tension of Belt which is used as the Force Transfer Media Induction Motor (유도 전동기의 동력전달매체로 사용되는 벨트의 장력 보상 알고리즘에 관한 연구)

  • 김승환;임무생
    • The Transactions of the Korean Institute of Electrical Engineers A
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    • v.48 no.9
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    • pp.1126-1130
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    • 1999
  • This paper persents an algorithm compensating for the change of tension acting on a belt which is used as a force transfer media of the induction motor that drives a washing machine. The induction motor adopted in a washing machine is operated not only for the power-transmission device, but also for the detection of clothes load. The load of clothes is determined by the duration of inertial rotation which is occurred by the induction motor during a specific time. The tension of belt also affects the determination of clothes load as another load and this change of the tension is a significant disturbance for accurate determination. This paper mentions the algorithm compensating for the amount of change in tension and the application of the algotithm proved to be effectively increasing the washing performance and reducing the noise and the vibration.

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Optimization of in vitro seed germination of Taraxacum platycarpum

  • Lee, Jung-Hwan;Kim, Young-Kwan;Oh, Eun-Yi;Jung, Kuk-Young;Ko, Ki-Sung
    • Korean Journal of Environmental Agriculture
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    • v.28 no.4
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    • pp.403-408
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    • 2009
  • Dandelion (Taraxacum platycarpum) has been widely utilized for medicinal purposes. However, the dandelion seeds are relatively difficult to germinate under cultivation conditions, which hampers seedling propagation of dandelion plants and reduces the opportunity of usage of such a useful medicinal plant. Thus, in this study, in vitro conditions for the dandelion seed germination were optimized to enhance the germination rate. In seed washing steps, the sequential treatments with 20% of ethanol, 20% of NaOCl, and distilled water avoided microbial contamination with the highest in vitro germination rate (67.5%) from seeds sown in germination media. The media supplemented with 1.4 g/L of MS salts and 1% of sucrose significantly enhanced the germination rate compared to the media with 4.4 g/L of MS and 3% of sucrose. Sowing the seeds vertically in the optimized media supplement conditions, 1.4 g/L of MS salts and 1% of sucrose, gave the maximum in vitro germination rate (61%), which was almost three times higher than sowing seeds on a soil pot (23%). Our results indicate that the seed washing and sowing methods including germination medium supplements can be optimized to enhance in vitro seed germination of dandelion.

Remediation of Contaminated Soil with Organic Contaminants using Microemulsion (마이크로이멀젼을 이용한 유기오염물로 오염된 지반의 정화)

  • Park, Ki-Hong;Kwon, Oh-Jung;Park, Jun-Boum
    • Proceedings of the Korean Geotechical Society Conference
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    • 2003.03a
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    • pp.597-604
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    • 2003
  • In the soil washing process, the contaminants are usually removed by abrasion from soil particles using mechanical energy and water However, organic contaminants with low water solubility like polycyclic aromatic hydrocarbons (PAH) are remained on soil particles. Previous studies have shown that surfactant possessing amphipathic activity enhances the solubility of organic materials. For this reason solutions with surfactants have been used to improve removal of organic contaminants on soil washing process. But, in this manner, many problems were found like complete loss of surfactants and additional contamination by surfactant. The remediation method using microemulsion has been introduced to overcome these disadvantages. In this case, surfactants are recycled by phase separation of microemulsion after remediation. In microemulsion process, the surfactant will be recycled by phase separation of the microemulsion into a surfactant-rich aqueous phase and an oil phase after extraction. That is why remediation concept applying microemulsion as washing media has been Introduced. Suitable microemulsion have to be used in order to have the chance of refilling the soil after decontamination and to avoid any risk due to toxicity. The purpose of this research is to evaluate effect of microemulsion to remediation of contaminated soil. We performed test with various organic contaminants like Pyrene and BTEX, also compared efficiency of remediation in microemulsion process with soil washing

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Analysis of Membrane Integrity and Mitochondrial Activity in Fresh and Cryopreserved Boar Sperm Using Flow Cytometry

  • Park C. S.;Li Z. H.;Sung N. D.;Jin D. I.;Cong P. Q.;Kim E. S.;Song E. S.;Yi Y. J.
    • Reproductive and Developmental Biology
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    • v.29 no.4
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    • pp.253-257
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    • 2005
  • This study was carried out to evaluate the effects of washing medium, breed and washing temperature of fresh and frozen-thawed boar sperm on mitochondrial activity and membrane integrity by flow cytometry. More than $80\%$ of fresh sperm washed with mTLP-PVA medium at $20^{\circ}C$ exhibited an intact membrane and a functional mitochondrion. With frozen-thawed samples, a large number of sperm showed both damaged membrane $(36.4\~46.9\%)$ and nonfunctional mitochondrion $(55.1\~71.1\%)$ in the mTLP-PVA and BTS washing media at $20^{\circ}C$. There were no breed effects of fresh and frozen-thawed sperm on mitochondrial activity and membrane integrity. The percentages of damaged membrane of fresh and frozen sperm, respectively, were higher at $4^{\circ}C$ washing temperature than at $20^{\circ}C$ washing temperature in the mTLP-PVA medium. We found that washing medium and washing temperature of fresh and frozen-thawed boar sperm were important for the analyses of mitochondrial activity and membrane integrity by flow cytometry.

In vitro Fertilization and Development of Pig Oocytes Inseminated with Boar Sperm by Different Sperm Washing Media after Thawing of the Frozen Straws

  • Yi, Y.J.;Ko, H.J.;Lee, S.H.;Yang, C.B.;Son, D.S.;Kim, H.K.;Park, C.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.2
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    • pp.164-167
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    • 2004
  • This study was carried out to investigate in vitro fertilization and development of in vitro matured pig oocytes inseminated with the Duroc boar sperm by different sperm washing media after thawing of the 5 ml frozen straws. Immature follicular oocytes (30-40) were transferred into each well of a Nunc 4-well multidish containing $500{\mu}l$ mTCM199 maturation medium. The sperm rich portion of ejaculates was collected into a 250 ml insulated vacuum bottle and gradually cooled 22 to $24^{\circ}C$ over a 2 h period. Semen was centrifuged at 800 g for 10 min and the seminal plasma discarded. Sperm were esuspended in a lactose-egg yolk and N-acetyl-Dglucosamine (LEN) diluent to contain $1{\times}10^{9}$ sperm/ml and cooled to $5^{\circ}C$ over a 2 h period. Immediately before freezing, semen was rediluted with an equal volume of LEN+4% glycerol and packed into 5 ml straws. After thawing of the 5 ml straw, the 5 ml semen was diluted with 20 ml Beltsville thawing solution (BTS) at room temperature. Oocytes were inseminated with untreated (unwashed and nonpreincubated) or treated sperm (washed two times in BTS, mTLP-PVA and mTBM media, respectively and nonpreincubated) with $2{\times}10^{7}$ sperm concentration. Oocytes were coincubated for 6 h in $500{\mu}l$ mTBM fertilization. At 6 h after IVF, oocytes were transferred into $500{\mu}l$ NCSU-23 culture medium for further culture of 6 h. Sperm penetration, polyspermy and male pronuclear formation of oocytes at 12 h after IVF and developmental ability of oocytes at 48 h after IVF were evaluated. Sperm penetration rate, male pronuclear formation and rate of cleaved embryos were higher in the BTS, mTLP-PVA and mTBM treatments than the unwashed treatment (p<0.05). The rate of blastocysts from the cleaved oocytes (2-4 cell stage) were higher in the mTLP-PVA treatment than in the unwashed, BTS and mTBM treatments. In conclusion, we recommend the washing of frozen-thawed sperm with mTLP-PVA medium before in vitro fertilization of oocytes in mTBM medium.

Culture Characteristics of Streptomyces spp. on Improved Polyacrylamide Gel and Agar Media

  • Han, Hong ui;Baek, Ji-Ho;Yang, Moon
    • Journal of Microbiology
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    • v.34 no.4
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    • pp.384-386
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    • 1996
  • Application of polyacrylamide gel (PAG) instead of agar to solid cultures of Streptomyces spp. was studied. The improved media were prepared by 1) gelling 20 ml of 5% acrylamide in a glass petri dish at room temperature, 2) washing by running water for more than 8 hr to remove residual reaction reagents, 3) drying at 5$0^{\circ}C$ for 12 hr to make a gel film, 4) autoclaving at 121$^{\circ}C$ for 15 min, and 5) swelling gel for about 4 hr by adding sterile liquid medium. In PAG media there were no differences from the observation of morphological characteristics showing during the cellular differentiation on agar media, whereas the ability to utilize carbohydrates differed somewhat from agar media. Agar media thus were little favorable for biochemical tests which the growth was determined depending on the formation of colony, but washed PAG was superior to serve as a solidifying agent.

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Fungi-rice bran based Fermentation of Coptis Chinensis and Curcuma Longa Root and its Influence of Silk Dyeing

  • Park, Young Mi;Choi, Jae Hong
    • Fashion & Textile Research Journal
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    • v.15 no.4
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    • pp.635-641
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    • 2013
  • This study examined the dye-properties of natural fabrics dyed with Coptis chinensis and Curcuma longa root fermented with fungi. The optimum culture conditions for the fermentation of microorganisms, the relationship between natural dye color and fermentation conditions were investigated. Two different medical herbs (ground to 80-100 mesh in size) were used as a natural dyeing source. Phellinus linteus (P. linteus), which can grow in different media, such as Agarmedium (only agar containing medium), maltose extract agar (MA) and potato dextrose extract agar (PDA) culture media, were isolated from the medium. P. linteus was confirmed to be the optimum microorganism for the fermentation of Coptis chinensis and Curcuma longa, and the MA medium was confirmed to be the best for culturing. When using the microorganism as the fermenting agent, $32^{\circ}C$ was found to be the optimum fermenting temperature for both natural colorants. Regarding the dyeing property of the fermented natural dye, silk was dyed quite darkly in an appearance by naked eye estimation and the K/S value in the color strength of silk reached a high level of 16 after the fermenting process. The washing fastness of dyed silk after treatment washing was reduced from 4 to under4 and indicates that dyed silk with fermented plant was not unsubstantial. The light fastness was 1 to 2, showing intended to maintain due to the fermentation process.

Removal of Chlorine from Fly Ash in Municipal Solid Waste Incineration Ash by Water Washing (수세에 의한 생활폐기물 소각재 중 비산재로부터 염소성분의 제거)

  • 안지환;한기천;김형석
    • Resources Recycling
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    • v.10 no.5
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    • pp.36-43
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    • 2001
  • The chlorine component in fly ash from municipal solid waste incineration ash was removed by water washing for the purpose of recycling fly ash as a raw material of ordinary portland cement. The samples were a different kind of 리y ashes using $Ca(OH)_2$and NaOH as media of wet scrubber for flue gas cleaning. The content of soluble salts of fly ash using $Ca(OH)_2$and NaOH was 32.8%, 50.1% and the content of chlorine component, 22.9% and 26.0% respectively, which was KCl, NaCl, CaC1OH mainly. When each fly ash was washed using water under conditions of a agitation speed of 300 rpm, a liquid to solid ratio of 10, most soluble salts in fly ash were dissolved within 30 minutes and the content of chlorine component in ash was diminished to the content of 4.4%, 2.O% at $20^{\circ}C$ and 1.7%, 0.8% at $50^{\circ}C$ respectively. And the main compound of residual chlorine component in ash after water washing was friedel`s salt ($3CaO.A1_2$$O_3$.$CaCl_2$.$10H2$O). From analysis results of water quality for wastewater by water washing, the components exceeding discharged wastewater standard were only Pb and Cd. But As pH was controlled to 10 with addition of $CO_2$(g) or $Na_2$$_CO3$in water, the concentration of heavy metals such as Pb and Cd was also under discharged wastewater standard.

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Effect of the Shape and Size of Quorum-Quenching Media on Biofouling Control in Membrane Bioreactors for Wastewater TreatmentS

  • Lee, Seonki;Lee, Sang Hyun;Lee, Kibaek;Kwon, Hyeokpil;Nahm, Chang Hyun;Lee, Chung-Hak;Park, Pyung-Kyu;Choo, Kwang-Ho;Lee, Jung-Kee;Oh, Hyun-Suk
    • Journal of Microbiology and Biotechnology
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    • v.26 no.10
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    • pp.1746-1754
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    • 2016
  • Recently, spherical beads entrapping quorum quenching (QQ) bacteria have been reported as effective moving QQ-media for biofouling control in MBRs for wastewater treatment owing to their combined effects of biological (i.e., quorum quenching) and physical washing. Taking into account both the mass transfer of signal molecules through the QQ-medium and collision efficiencies of the QQ-medium against the filtration membranes in a bioreactor, a cylindrical medium (QQ-cylinder) was developed as a new shape of moving QQ-medium. The QQ-cylinders were compared with previous QQ-beads in terms of the QQ activity and the physical washing effect under identical loading volumes of each medium in batch tests. It was found that the QQ activity of a QQ-medium was highly dependent on its specific surface area, regardless of the shape of the medium. In contrast, the physical washing effect of a QQ-medium was greatly affected by its geometric structure. The enhanced anti-biofouling property of the QQ-cylinders relative to QQ-beads was confirmed in a continuous laboratory-scale MBR with a flat-sheet membrane module.