• Title/Summary/Keyword: Wall-function

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Characterization of an Apple Polygalacturonase-Inhibiting Protein (PGIP) That Specifically Inhibits an Endopolygalacturonase (PG) Purified from Apple Fruits Infected with Botryosphaeria dothidea

  • Lee Dong-Hoon;Bae Han-Hong;Kang In-Kyu;Byun Jae-Kyun;Kang Sang-Gu
    • Journal of Microbiology and Biotechnology
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    • v.16 no.8
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    • pp.1192-1200
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    • 2006
  • An apple polygalacturonase-inhibiting protein (PGIP), which specifically inhibits endopolygalacturonase (PG, EC 3.2.1.15) from Botryosphaeria dothidea, was purified from Botryosphaeria dothidea-infected apple (Malus domestica cv. Fuji) fruits. The purified apple PGIP had a molecular mass of 40 kDa. The N-terminal amino acid sequence of the purified protein showed high homologies to those of PGIP from pear (100%), tomato (70%), and bean (65%). We also purified polygalacturonase (PG) from B. dothidea. The PG hydrolyzes pectic components of plant cell walls. When the extracted apple pectic cell wall material was treated with purified apple PGIP and B. dothidea PG, the amount of uronic acid released was lower than that treated with B. dothidea PG alone. This result demonstrates that PGIP functions specifically by inhibiting cell wall maceration of B. dothidea PG Furthermore, we characterized the de novo function of the PGIP against PG on the solubilization and depolymerization of polyuronides from cell wall of apple fruits inoculated with B. dothidea. This result demonstrated that the PGIP of plants exhibits one of the direct defense mechanisms against pathogen attack by inhibiting PGs that are released from pathogens for hydrolysis of cell wall components of plants.

The Effects of Penicillin on the growth of Bacillus subtilis (Penicillin이 Bacillus subtilis의 생육에 미치는 영향)

  • 홍순덕
    • Microbiology and Biotechnology Letters
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    • v.1 no.1
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    • pp.19-23
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    • 1973
  • This experiment was done to investigate the growth and the fine structural changes of Bacillus subtilis which were influenced by the penicillin with electromicroscope. The results were as follows; 1) The higher the concentration of penicillin the more prominent inhibition of the growth was observed. 2) The septa was not formed, derangements of synthesis of cell wall and cell membrane. 3) Cytoplasm was increased with swelling of cell body because of weakness of cell membrane induced by deranged synthesis of cell membrane. Some of the cells showed disruption of their membrane with loss of cytoplasm, remaining empty space, which suggest loss of cell function. 4) It can be suggested that penicillin had affected on the cell wall of Bacillus subtilis, and inhibited growth of the cell by deranging the formation of the cell wall.

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A CFD Prediction of a Micro Critical Nozzle (마이크로 임계노즐 유동의 CFD 예측)

  • 김재형;김희동;박경암
    • Journal of the Korean Society of Propulsion Engineers
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    • v.7 no.2
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    • pp.7-14
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    • 2003
  • Computational work using the axisymmetric, compressible, Navier-Stokes Equations is carried out to predict the discharge coefficient of mass flow through a micro-critical nozzle. Several kinds of turbulence models and wall functions are employed to validate the computational predictions. The computed results are compared with the previous experimented ones. The present computations predict the experimental discharge coefficients with a reasonable accuracy. It is found that the standard $\kappa$-$\varepsilon$turbulence model with the standard wall function gives a best prediction of the discharge coefficients. The displacement thickness of the nozzle wall boundary layer is evaluated at the nozzle throat and is well compared to a prediction obtained by an empirical equation. The resulting displacement thickness of the wall boundary layer is about 2% to 0.6% of the diameter of the nozzle throat for the Reynolds numbers of 2000 to 20000.

Shear Load Performance Test in Accordance with Sheathing Materials of Shear Wall (전단벽의 덮개재료에 따른 전단저항 성능)

  • Jang, Sang-Sik;Shin, Il-Joong;Kim, Yun-Hui
    • Korean Journal of Agricultural Science
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    • v.37 no.2
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    • pp.271-276
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    • 2010
  • In this study, the light-frame wood shear walls according to the sheathing materials was carried out to investigate the shear load performance. Most common sheathing materials are the structural OSB and gypsum board used to consist wall of wood-frame house. Seven different type of specimens are composed of several sheathing materials and shear test was taken to evaluate shear performance by KS F 2154. As a result, shear walls(G12.5/G12.5 and G12.5/OSB) show that maximum shear strength and shear rigidity modulus are 7316N/mm${\cdot}$118.25 N/mm and 11129 N/mm${\cdot}$184.66 N/mm respectively. The shear wall using gypsum board 15mm improve maximum shear strength and shear rigidity modulus about 30%. The shear wall using 15mm gypsum board showed intermediate value in one side specimens. Different types of shear walls could be compared with the shear load performance. Also, nailed joint failure aspects are different to sheathing material and installing method.

A Study on the Interior Design and Wall Performance Optimizing Method by Using GA and AHP (GA와 AHP를 이용한 실내 디자인과 벽체 성능 최적화 방법에 관한 연구)

  • 진경일;이경회
    • Korean Institute of Interior Design Journal
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    • no.29
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    • pp.86-93
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    • 2001
  • This study presents about the method of alternatives selection by considering wall performance and interior design. Wall is selected fur the object and 3 items of cost, performance, and design as the objective function for optimizing are determined. Thus the wall performance selected problems, which are improvement of insulation performance, sweaty prevention, sound insulation performance and design selected problems, which is satisfactory Improvement of users about Interior design. It is important to select alternatives that can satisfy the performance and design on the capital given as much as possible. But quantitative problem such as performance or expanses and qualitative problem such as design are not in the same dimension. Therefore this problem is a multi-criteria optimization problem and also has used AHP method as the method to solve these. Moreover GA is used to solve a problem of the alternatives occurrence, which is the characteristic of multi-criteria problem. This study presents the solution method on multi-criteria problem that has been mix loaded of quantitative problem and qualitative problem by using AHP(Analytic Hierarchy Process) and GA(Genetic Algorithm).

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Experimental investigation of a method for diagnosing wall thinning in an artificially thinned carbon steel elbow based on changes in modal characteristics

  • Byunyoung Chung ;Jonghwan Kim ;Daesic Jang;Sunjin Kim;Youngchul Choi
    • Nuclear Engineering and Technology
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    • v.55 no.3
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    • pp.947-957
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    • 2023
  • Curved cylindrical structures such as elbows have a non-uniform thickness distribution due to their fabrication process, and as a result have a number of complex mode shapes, including circumferential and axial nodal patterns. In nuclear power plants, material degradation is induced in pipes by flow accelerated erosion and corrosion, causing the wall thickness of carbon steel elbows to gradually thin. The corresponding frequencies of each mode shape vary according to the wall thinning state. Therefore, the thinning state can be estimated by monitoring the varying modal characteristics of the elbow. This study investigated the varying modal characteristics of artificially thinned carbon steel elbows for each thinning state using numerical simulation and experimental methods (MRIT, Multiple Reference Impact Test). The natural frequencies of specified mode shapes were extracted, and results confirmed they linearly decreased with increasing thinning. In addition, by comparing single FRF (Frequency Response Function) data with the results of MRIT, a concise and cost effective thinning estimation method was suggested.

Analyzing the factors that contribute to the development of embryological classical type of bladder exstrophy

  • Ria Margiana;Widya Juwita;Khoirul Ima;Zakiyatul Faizah;Supardi Supardi
    • Anatomy and Cell Biology
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    • v.56 no.4
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    • pp.421-427
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    • 2023
  • Bladder exstrophy is a rare congenital condition of the pelvis, bladder, and lower abdomen that opens the bladder against the abdominal wall, produces aberrant growth, short penis, upward curvature during erection, wide penis, and undescended testes. Exstrophy affects 1/30,000 newborns. The bladder opens against the abdominal wall in bladder exstrophy, a rare genitourinary condition. This study is vital to provide appropriate therapy choices as a basis to improve patient outcomes. This study may explain bladder exstrophy and provide treatment. Epispadias, secretory placenta, cloacal exstrophy, and other embryonic abnormalities comprise the exstrophy-spades complex. The mesenchymal layer does not migrate from the ectoderm and endoderm layers in the first trimester, affecting the cloacal membrane. Embryological problems define the exstrophy-aspidistra complex, which resembles epimedium, classic bladder, cloacal exstrophy, and other diseases. Urogenital ventral body wall anomalies expose the bladder mucosa, causing bladder exstrophy. Genetic mutations in the Hedgehog cascade pathway, Wnt signal, FGF, BMP4, Alx4, Gli3, and ISL1 cause ventral body wall closure and urinary bladder failure. External factors such as high maternal age, smoking moms, and high maternal body mass index have also been associated to bladder exstrophy. Valproic acid increases bladder exstrophy risk; chemicals and pollutants during pregnancy may increase bladder exstrophy risk. Bladder exstrophy has no identified cause despite these risk factors. Exstrophy reconstruction seals the bladder, improves bowel function, reconstructs the vaginal region, and restores urination.

Wall Cuckoo: A Method for Reducing Memory Access Using Hash Function Categorization (월 쿠쿠: 해시 함수 분류를 이용한 메모리 접근 감소 방법)

  • Moon, Seong-kwang;Min, Dae-hong;Jang, Rhong-ho;Jung, Chang-hun;NYang, Dae-hun;Lee, Kyung-hee
    • KIPS Transactions on Computer and Communication Systems
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    • v.8 no.6
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    • pp.127-138
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    • 2019
  • The data response speed is a critical issue of cloud services because it directly related to the user experience. As such, the in-memory database is widely adopted in many cloud-based applications for achieving fast data response. However, the current implementation of the in-memory database is mostly based on the linked list-based hash table which cannot guarantee the constant data response time. Thus, cuckoo hashing was introduced as an alternative solution, however, there is a disadvantage that only half of the allocated memory can be used for storing data. Subsequently, bucketized cuckoo hashing (BCH) improved the performance of cuckoo hashing in terms of memory efficiency but still cannot overcome the limitation that the insert overhead. In this paper, we propose a data management solution called Wall Cuckoo which aims to improve not only the insert performance but also lookup performance of BCH. The key idea of Wall Cuckoo is that separates the data among a bucket according to the different hash function be used. By doing so, the searching range among the bucket is narrowed down, thereby the amount of slot accesses required for the data lookup can be reduced. At the same time, the insert performance will be improved because the insert is following up the operation of the lookup. According to analysis, the expected value of slot access required for our Wall Cuckoo is less than that of BCH. We conducted experiments to show that Wall Cuckoo outperforms the BCH and Sorting Cuckoo in terms of the amount of slot access in lookup and insert operations and in different load factor (i.e., 10%-95%).

Finite Element Analysis of Natural Convection of Fluids with Low Prandtl Number in a Square Enclosure (유한요소법을 이용한 정방형 밀폐용기내의 플란틀수가 낮은 유체의 자연대류에 관한 연구)

  • 김무현;이진호;강신형;손영석
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.12 no.3
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    • pp.541-550
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    • 1988
  • Natural convection in a square enclosure was investigated numerically for low prandtl number fluids using Finite Element Method. In case of Ra=10$^{4}$, 10$^{5}$ and 10$^{6}$ the temperature gradient decreases gradually at the lower end of the hot wall(or at the upper end of the cold wall) as prandtl number decreases in the range of 0.01 .leq. Pr .leq. 10. Maximum heat transfer occurs at a somewhat higher point from the lower end of hot wall(or at somewhat lower point from the upper end of the cold wall) and it draws near to the lower end of the hot wall(or draws near to the upper end of the cold wall) with increasing prandtl number. The flow in the enclosure appears as an Unicell Pattern for Ra .leq. 10$^{4}$ and secondarily flows(or tertiary flows) appears in the core region for Ra .geq. 10$^{5}$ . The line joining the center of secondary cells skewes in a clockwise direction as the Prandtl number decreases.

Seismic response and damage development analyses of an RC structural wall building using macro-element

  • Hemsas, Miloud;Elachachi, Sidi-Mohammed;Breysse, Denys
    • Structural Engineering and Mechanics
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    • v.51 no.3
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    • pp.447-470
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    • 2014
  • Numerical simulation of the non-linear behavior of (RC) structural walls subjected to severe earthquake ground motions requires a reliable modeling approach that includes important material characteristics and behavioral response features. The objective of this paper is to optimize a simplified method for the assessment of the seismic response and damage development analyses of an RC structural wall building using macro-element model. The first stage of this study investigates effectiveness and ability of the macro-element model in predicting the flexural nonlinear response of the specimen based on previous experimental test results conducted in UCLA. The sensitivity of the predicted wall responses to changes in model parameters is also assessed. The macro-element model is next used to examine the dynamic behavior of the structural wall building-all the way from elastic behavior to global instability, by applying an approximate Incremental Dynamic Analysis (IDA), based on Uncoupled Modal Response History Analysis (UMRHA), setting up nonlinear single degree of freedom systems. Finally, the identification of the global stiffness decrease as a function of a damage variable is carried out by means of this simplified methodology. Responses are compared at various locations on the structural wall by conducting static and dynamic pushover analyses for accurate estimation of seismic performance of the structure using macro-element model. Results obtained with the numerical model for rectangular wall cross sections compare favorably with experimental responses for flexural capacity, stiffness, and deformability. Overall, the model is qualified for safety assessment and design of earthquake resistant structures with structural walls.