• Title/Summary/Keyword: WD40

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Development of Screw-Type Handy Earth Auger for an Improved Digging Efficiency(I) - Design and Manufacture - (토양굴취력이 향상된 스크류형 경량 식혈기 개발(I) - 설계 및 제작 -)

  • Kim, Jin Hyun;Lee, Jae Hyun;Kim, Ki Dong;Ko, Chi Woong;Kim, Dong Geun
    • Journal of agriculture & life science
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    • v.50 no.3
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    • pp.31-41
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    • 2016
  • This study was conducted to develop a handy earth auger for use in sloppy and rugged forest terrains in order to reduce labor cost which comprises a major part of the production costs in forest afforestation projects. The first prototype is developed consist of two parts, the soil-digging screw and the battery power source. The specifications of the first prototype screw are: length of 170mm, a top diameter of 60mm, bottom diameter of 47mm, 23° angle for each helix, and a 50mm awl-head tip. The use of a single line of screw was selected for reduced weight. In addition, a power source of rotary DC Motor(WD-6G2425, WONILL, Korea) with a maximum torque of 30kgf-cm, rotation of 20-30rpm, K6G30C decelerator with a reduction ratio of 30:1 which could be used with no load for 48 was operated. In consideration of its weight, a lithium battery was utilized in line with the goal of developing a lightweight auger. In order to evaluate the performance of the first prototype, test sites were selected as 6 areas. The rotational force was found to be highest in area A(Solid area), followed by areas F(Mounted slope 40° area) and E(Mounted slope 30° area). It was also observed that in general, the rotational force increased along with the increase in soil depth with the maximum rotational force recorded at 10cm.

Biochemical and molecular features of LRRK2 and its pathophysiological roles in Parkinson's disease

  • Seol, Won-Gi
    • BMB Reports
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    • v.43 no.4
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    • pp.233-244
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    • 2010
  • Parkinson's disease (PD) is the second most common neurodegenerative disease, and 5-10% of the PD cases are genetically inherited as familial PD (FPD). LRRK2 (leucine-rich repeat kinase 2) was first reported in 2004 as a gene corresponding to PARK8, an autosomal gene whose dominant mutations cause familial PD. LRRK2 contains both active kinase and GTPase domains as well as protein-protein interaction motifs such as LRR (leucine-rich repeat) and WD40. Most pathogenic LRRK2 mutations are located in either the GTPase or kinase domain, implying important roles for the enzymatic activities in PD pathogenic mechanisms. In comparison to other PD causative genes such as parkin and PINK1, LRRK2 exhibits two important features. One is that LRRK2's mutations (especially the G2019S mutation) were observed in sporadic as well as familial PD patients. Another is that, among the various PD-causing genes, pathological characteristics observed in patients carrying LRRK2 mutations are the most similar to patients with sporadic PD. Because of these two observations, LRRK2 has been intensively investigated for its pathogenic mechanism (s) and as a target gene for PD therapeutics. In this review, the general biochemical and molecular features of LRRK2, the recent results of LRRK2 studies and LRRK2's therapeutic potential as a PD target gene will be discussed.

Identification of WDR7 as a Novel Downstream Target of the EphA8-Odin Signaling Complex

  • Park, Eun-Jeong;Park, Soo-Chul
    • Animal cells and systems
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    • v.13 no.1
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    • pp.9-15
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    • 2009
  • Eph receptors and their ephrin ligands have been implicated in a variety of cellular processes such as cellular morphogenesis and motility. Our previous studies demonstrated that Odin, one of the Anks family proteins, functions as a scaffolding protein of the EphA8 signaling pathway leading to modulation of cell migration or axonal outgrowth. Here we show that WDR7 is associated with Odin and that it is possibly implicated in the EphA8 signaling pathway. WD40 repeats present in the COOH-terminal region of WDR7 appear to be crucial for its association with Odin, whereas the binding motif of Odin is located in between ankyrin repeats and PTB domain. Co-immunoprecipitation experiments revealed that association of WDR7 with Odin is enhanced by ephrin ligand treatment, possibly through forming large protein complexes including both EphA8 and ephrin-A5. Consistently, immunofluorescence staining experiments suggested that WDR7 constitute a component of the large protein complexes containing Odin, EphA8 and ephrin-A5. Taken together, our results suggest the WDR7-Odin complexes might be involved in the signaling pathway downstream of the EphA8 receptor.

The Deformation and Breaking Load of the Fishing Hook by the Tensile Test (인장시험에 의한 낚시의 변형과 파단하중)

  • KO Kwan-Soh;KIM Yong-Hae
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.14 no.4
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    • pp.269-275
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    • 1981
  • The fishing hooks were tested for breaking and unbending due to plastic deformation of the material. Study of tensile test is not complicated, but has not even worked out fully enough, especially when the test specimen is subjected to plastic deformation. The fishing hook is subjected to unbending stress and the critical section is a Point which is furthest from the line of action of the forces. The dynamic force of fish during jerks depends on their speed of movement and body weight, the kinetic energy corresponding to it and also on the rlastic displacement of the rigging which absorb the energy. Six kinds of hook were tested by the dynamometer under tensile speed 290mm/min (subscript s) and 780mm/min (subscript f). According to their results, the breaking load(B: kg) can be induced with the formula $B={\alpha}wd^2+\beta$ where w(mm) is the distance between the barb base and the lower shank and d(mm) is diameter. The coefficients of the formula for the round hooks(R) and the angular hooks(A) are approximately as follows: $$R:\;\alpha_{s}=0.5,\;\beta_{s}=1.6,\;\alpha_{f}=0.4,\;\beta_{f}=1.4$$ $$A:\;\alpha_{s}=1.1,\;\beta_{s}=2.0,\;\alpha_{f}=1.0,\;\beta_{f}=0.9$$ The ratio of $B_{f}\;to\;B_{s}$ is corresponding to 0.8. The ratio of deformation(X) that is moved distance of barb base at break to the distance(H) between head base and barb base is about $50\%$. Further study should be carried out on the subject of impact and fatigue test under the same condition which is exerted force by the hooked fish.

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Analysis of Power Shuttle Characteristics of Agricultural Tractor (농업용 트랙터의 전후진 파워시프트 변속 특성 해석)

  • 김대철;이호상;정병학;김경욱
    • Journal of Biosystems Engineering
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    • v.27 no.6
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    • pp.479-490
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    • 2002
  • A dynamic model of a power shuttle transmission was developed and its validity was verified using the experimental data obtained from a transmission test bench. A 40㎾, 4WD tractor was also modeled using an application software EASY5 to investigate parameters and their effects on the power shifting performance. For a tractor model, the manual reverse gear was replaced by a power shuttle transmission. The tractor model also included an engine, main-gears for transmission, wheels, differentials and planet gears. Using the tractor model, the effects of the parameters such as modulating pressure and time, engine speed, tractor speed. tractor weight. reverse to forward speed ratio and torsional damper on the transient characteristics at starting and shuttle shifting were investigated by the computer simulation. The transient characteristics were represented by variations in clutch pressure, torque transmitted to input shaft and driving wheels, and power transmission capacity of the clutch. It was found that the modulating pressure and time affected most significantly the torque transmission and shifting time. The input torque, axle torque, power transmission capacity of the clutch and transmission time all increased with increase in engine speed, tractor speed. tractor weight and ratio of reverse to forward speeds. However, the axle torque decreased with tractor speed. Both the axle torque and power transmission capacity of the clutch also decreased with the ratio of reverse to forward speeds.

Cloning and Expression Characteristics of Pharbitis nil COP1 (PnCOP1) During the Floral Induction

  • Kim, Yun-Hee;Kim, Seong-Ryong;Heo, Yoon-Kang
    • Journal of Photoscience
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    • v.12 no.1
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    • pp.1-9
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    • 2005
  • The ubiquitin E3 ligase COP1 (Constitutive Photomorphogenesis 1) is a protein repressor of photomorphogenesis in Arabidopsisplants, and it found in various organisms, including animals. The COP1 protein regulates the stability of many of the light-signaling components that are involved in photomorphogenesis and in the developmental processes. To study the effect of COP1 on flowering in a short day plant, we have cloned a full-length of PnCOP1 (Pharbitis nil COP1) cDNA from Pharbitis nil Choisy cv. Violet, and we examined its transcript levels under various conditions. A full-length PnCOP1 cDNA consists of 2,280 bp nucleotidesthat contain 47 bp of 5'-UTR, 232 bp of 3'-UTR including the poly (A) tail, and 1,998 bp of the coding sequence. The deduced amino acid sequence contains 666 amino acids, giving it a theoretical molecular weight of 75 kD and a isolectric point of 6.2. The PnCOP1 contains three distinct domains, an N-terminal $Zn^2+$-binding RING-finger domain, a coiled-coil structure, and WD40 repeats at the C-terminal, implying that the protein plays a role in protein-protein interactions. The PnCOP1 transcript was detected in the cotyledon, hypocotyls and leaves, but not in root. The levels of the PnCOP1 transcript were reduced in leaves that were a farther distance away from the cotyledons. The expression level of the PnCOP1 gene was inhibited by light, while the expression was increased in the dark. During the floral inductive 16 hour-dark period for Pharbitis nil, the expression was increased and it reached its maximum at the 12th hour of the dark period. The levels of PnCOP1 mRNA were dramatically reduced upon light illumination. These results suggest that PnCOP1 may play an important function in the floral induction of Pharbitis nil.

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Type-I Hypersensitivity to Malassezia pachydermatis Extracts in Healthy Dogs and Dogs with Malasseza Otitis Externa (정상개와 Malassezia 외이염을 가진 개에 있어서 Malassezia pachydermatis 추출물의 즉시형 과민반응)

  • Kim, Young-Sub;Lee, Keun-Woo;Oh, Tae-Ho
    • Journal of Veterinary Clinics
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    • v.24 no.2
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    • pp.88-93
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    • 2007
  • The purpose of the study reported here was to test the hypotheses that clinically healthy dogs will not manifest immediate hypersensitivity responses to intradermal injection of Malassezia pachydermatis extracts but that affected dogs with Malassesia otitis will manifest such hypersensitivity. Wd desired to identify approximate molecular mass of any allergenic components of the yeast by use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The protein profile of Malassezia pachydermatis extracts showed between 16 and 110 kDa. Especially, the intensity was strongest between 25 and 80 kDa. Mean wheal diameters in the affected groups of 20, 2, 0.2, and $0.02{\mu}g/ml$ were $13.36{\pm}0.67,\;5.33{\pm}0.67,\;5.47{\pm}0.82,\;and\;5.07{\pm}0.64$, respectively. Mean wheal thickness in the affected groups of 20, 2, 0.2, and $0.02{\mu}g/ml$ was $6.44{\pm}0.40,\;3.86{\pm}0.35,\;2.64{\pm}0.36,\;and\;2.60{\pm}0.44$, respectively. The difference of wheal diameters and thickness between healthy and affected groups was significant (p<0.05). In conclusion, the observations confirm that Malassezia pachydermatis-derived antigens may induce an immediate wheal response when intradermal injected in dogs. It seems reasonable to suggest that hypersensitivity to yeast may contribute to the development of clinical signs in dogs with immediate skin test reactivity, especially in dogs with Malassezia otitis extema.

Full-Length Enriched cDNA Library Construction from Tissues Related to Energy Metabolism in Pigs

  • Lee, Kyung-Tai;Byun, Mi-Jeong;Lim, Dajeong;Kang, Kyung-Soo;Kim, Nam-Soon;Oh, Jung-Hwa;Chung, Chung-Soo;Park, Hae-Suk;Shin, Younhee;Kim, Tae-Hun
    • Molecules and Cells
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    • v.28 no.6
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    • pp.529-536
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    • 2009
  • Genome sequencing of the pig is being accelerated because of its importance as an evolutionary and biomedical model animal as well as a major livestock animal. However, information on expressed porcine genes is insufficient to allow annotation and use of the genomic information. A series of expressed sequence tags of 5' ends of five full-length enriched cDNA libraries (SUSFLECKs) were functionally characterized. SUSFLECKs were constructed from porcine abdominal fat, induced fat cells, loin muscle, liver, and pituitary gland, and were composed of non-normalized and normalized libraries. A total of 55,658 ESTs that were sequenced once from the 5′ ends of clones were produced and assembled into 17,684 unique sequences with 7,736 contigs and 9,948 singletons. In Gene Ontology analysis, two significant biological process leaf nodes were found: gluconeogenesis and translation elongation. In functional domain analysis based on the Pfam database, the beta transducin repeat domain of WD40 protein was the most frequently occurring domain. Twelve genes, including SLC25A6, EEF1G, EEF1A1, COX1, ACTA1, SLA, and ANXA2, were significantly more abundant in fat tissues than in loin muscle, liver, and pituitary gland in the SUSFLECKs. These characteristics of SUSFLECKs determined by EST analysis can provide important insight to discover the functional pathways in gene networks and to expand our understanding of energy metabolism in the pig.